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PLK4 (NM_001190801) Human Tagged ORF Clone
CAT＃: RC230885
- TrueORF®
PLK4 (Myc-DDK-tagged)-Human polo-like kinase 4 (PLK4), transcript variant 3
ORF Plasmid: tGFP
"NM_001190801" in other vectors (3)
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CNY 9,690.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MCCRP2; SAK; STK18 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230885 representing NM_001190801
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCTGCATCGGGGAGAAGATCGAGGATTTTAAAGTTGGAAATCTGCTTGGTAAAGGATCATTTG CTGGTGTCTACAGAGCTGAGTCCATTCACACTGGTTTGGAAGTTGCAATCAAAATGATAGATAAGAAAGC CATGTACAAAGCAGGAATGGTACAGAGAGTCCAAAATGAGGTGAAAATACATTGCCAATTGAAACATCCT TCTATCTTGGAGCTTTATAACTATTTTGAAGATAGCAATTATGTGTATCTGGTATTAGAAATGTGCCATA ATGGAGAAATGAACAGGTATCTAAAGAATAGAGTGAAACCCTTCTCAGAAAATGAAGCTCGACACTTCAT GCACCAGATCATCACAGGGATGTTGTATCTTCATTCTCATGGTATACTACACCGGGACCTCACACTTTCT AACCTCCTACTGACTCGTAATATGAACATCAAGATTGCTGATTTTGGGCTGGCAACTCAACTGAAAATGC CACATGAAAAGCACTATACATTATGTGGAACTCCTAACTACATTTCACCAGAAATTGCCACTCGAAGTGC ACATGGCCTTGAATCTGATGTTTGGTCCCTGGGCTGTATGTTTTATACATTACTTATCGGGAGACCACCC TTCGACACTGACACAGTCAAGAACACATTAAATAAAGTAGTATTGGCAGATTATGAAATGCCATCTTTTT TGTCAATAGAGGCCAAGGACCTTATTCACCAGTTACTTCGTAGAAATCCAGCAGATCGTTTAAGTCTGTC TTCAGTATTGGACCATCCTTTTATGTCCCGAAATTCTTCAACAAAAAGTAAAGATTTAGGAACTGTGGAA GACTCAATTGATAGTGGGCATGCCACAATTTCTACTGCAATTACAGCTTCTTCCAGTACCAGTATAAGTG GTAGTTTATTTGACAAAAGAAGACTTTTGATTGGTCAGCCACTCCCAAATAAAATGACTGTATTTCCAAA GAATAAAAGTTCAACTGATTTTTCTTCTTCAGGAGATGGAAACAGTTTTTATACTCAGTGGGGAAATCAA GAAACCAGTAATAGTGGAAGGGGAAGAGTAATTCAAGATGCAGAAGAAAGGCCACATTCTCGATACCTTC GTAGAGCTTATTCCTCTGATAGATCTGGCACTTCTAATAGTCAGTCTCAAGCAAAAACATATACAATGGA ACGATGTCACTCAGCAGAAATGCTTTCAGTGTCCAAAAGATCAGGAGGAGGTGAAAATGAAGAGAGGTAC TCACCCACAGACAACAATGCCAACATTTTTAACTTCTTTAAAGAAAAGACATCCAGTAGTTCTGGATCTT TTGAAAGACCTGATAACAATCAAGCACTCTCCAATCATCTTTGTCCAGGAAAAACTCCTTTTCCATTTGC AGACCCGACACCTCAGACTGAAACCGTACAACAGTGGTTTGGGAATCTGCAAATAAATGCTCATTTAAGA AAAACTACTGAATATGACAGCATCAGCCCAAACCGGGACTTCCAGGGCCATCCAGATTTGCAGAAGGACA CATCAAAAAATGCCTGGACTGATACAAAAGTCAAAAAGAACTCTGATGCTTCTGATAATGCACATTCTGT AAAACAGCAAAATACCATGAAATATATGACTGCACTTCACAGTAAACCTGAGATAATCCAACAAGAATGT GTTTTTGGCTCAGATCCTCTTTCTGAACAGAGCAAGACTAGGGGTATGGAGCCACCATGGGGTTATCAGA ATCGTACATTAAGAAGCATTACATCTCCGTTGGTTGCTCACAGGTTAAAACCAATCAGACAGAAAACCAA AAAGGCTGTGGTGAGCATACTTGATTCAGAGGAGGTGTGTGTGGAGCTTGTAAAGGAGTATGCATCTCAA GAATATGTGAAAGAAGTTCTTCAGATATCTAGTGATGGAAATACGATCACTATTTATTATCCAAATGGTG GTAGAGGTTTTCCTCTTGCTGATAGACCACCCTCACCTACTGACAACATCAGTAGGTACAGCTTTGACAA TTTACCAGAAAAATACTGGCGAAAATATCAATATGCTTCCAGGTTTGTACAGCTTGTAAGATCTAAATCT CCCAAAATCACTTATTTTACAAGATATGCTAAATGCATTTTGATGGAGAATTCTCCTGGTGCTGATTTTG AGGTTTGGTTTTATGATGGGGTAAAAATACACAAAACAGAAGATTTCATTCAGGTGATTGAAAAGACAGG GAAGTCTTACACTTTAAAAAGTGAAAGTGAAGTTAATAGCTTGAAAGAGGAGATAAAAATGTATATGGAC CATGCTAATGAGGGTCATCGTATTTGTTTAGCACTGGAATCCATAATTTCAGAAGAGGAAAGGAAAACTA GGAGTGCTCCCTTTTTCCCAATAATCATAGGAAGAAAACCTGGTAGTACTAGTTCACCTAAGGCCTTATC ACCTCCTCCTTCTGTGGATTCAAATTACCCAACGAGAGAGAGAGCATCTTTCAACAGAATGGTCATGCAT AGTGCTGCTTCTCCAACACAGGCACCAATCCTTAATCCCTCTATGGTTACAAATGAAGGACTTGGTCTTA CAACTACAGCTTCTGGAACAGACATCTCTTCTAATAGTCTAAAAGATTGTCTTCCTAAATCAGCACAACT TTTGAAATCTGTTTTTGTGAAAAATGTTGGTTGGGCTACACAGTTAACTAGTGGAGCTGTGTGGGTTCAG TTTAATGATGGGTCCCAGTTGGTTGTGCAGGCAGGAGTGTCTTCTATCAGTTATACCTCACCAAATGGTC AAACAACTAGGTATGGAGAAAATGAAAAATTACCAGACTACATCAAACAGAAATTACAGTGTCTGTCTTC CATCCTTTTGATGTTTTCTAATCCGACTCCTAATTTTCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230885 representing NM_001190801
Red=Cloning site Green=Tags(s) MATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHP SILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLS NLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPP FDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVE DSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQ ETSNSGRGRVIQDAEERPHSRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERY SPTDNNANIFNFFKEKTSSSSGSFERPDNNQALSNHLCPGKTPFPFADPTPQTETVQQWFGNLQINAHLR KTTEYDSISPNRDFQGHPDLQKDTSKNAWTDTKVKKNSDASDNAHSVKQQNTMKYMTALHSKPEIIQQEC VFGSDPLSEQSKTRGMEPPWGYQNRTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELVKEYASQ EYVKEVLQISSDGNTITIYYPNGGRGFPLADRPPSPTDNISRYSFDNLPEKYWRKYQYASRFVQLVRSKS PKITYFTRYAKCILMENSPGADFEVWFYDGVKIHKTEDFIQVIEKTGKSYTLKSESEVNSLKEEIKMYMD HANEGHRICLALESIISEEERKTRSAPFFPIIIGRKPGSTSSPKALSPPPSVDSNYPTRERASFNRMVMH SAASPTQAPILNPSMVTNEGLGLTTTASGTDISSNSLKDCLPKSAQLLKSVFVKNVGWATQLTSGAVWVQ FNDGSQLVVQAGVSSISYTSPNGQTTRYGENEKLPDYIKQKLQCLSSILLMFSNPTPNFH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001190801 |
| ORF Size | 2913 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001190801.1, NP_001177730.1 |
| RefSeq Size | 3693 bp |
| RefSeq ORF | 2790 bp |
| Locus ID | 10733 |
| UniProt ID | O00444 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 109 kDa |
| Gene Summary | This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230885L3 | Lenti-ORF clone of PLK4 (Myc-DDK-tagged)-Human polo-like kinase 4 (PLK4), transcript variant 3 |
CNY 11,590.00 |
|
| RC230885L4 | Lenti-ORF clone of PLK4 (mGFP-tagged)-Human polo-like kinase 4 (PLK4), transcript variant 3 |
CNY 11,590.00 |
|
| RG230885 | PLK4 (tGFP-tagged) - Human polo-like kinase 4 (PLK4), transcript variant 3 |
CNY 10,640.00 |
