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NFATC4 (NM_001198966) Human Tagged ORF Clone
CAT＃: RC230880
- TrueORF®
NFATC4 (Myc-DDK-tagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (NFATC4), transcript variant 4
ORF Plasmid: tGFP
"NM_001198966" in other vectors (3)
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CNY 8,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NF-AT3; NF-ATC4; NFAT3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230880 representing NM_001198966
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATTCGCCACCGCCGCGACCAGCCCCCTCACCTGGCACCTGGGAGAGCCAGCCCGCCAGGTCGGTGA GGCTGGGAGGACCAGGAGGGGGTGCTGGGGGTGCTGGGGGTGGCCGTGTTCTCGAGTGTCCCAGCATCCG CATCACCTCCATCTCTCCCACGCCGGAGCCGCCAGCAGCGCTGGAGGACAACCCTGATGCCTGGGGGGAC GGCTCTCCTAGAGATTACCCCCCACCAGAAGGCTTTGGGGGCTACAGAGAAGCAGGGGGCCAGGGTGGGG GGGCCTTCTTCAGCCCAAGCCCTGGCAGCAGCAGCCTGTCCTCGTGGAGCTTCTTCTCCGATGCCTCTGA CGAGGCAGCCCTGTATGCAGCCTGCGACGAGGTGGAGTCTGAGCTAAATGAGGCGGCCTCCCGCTTTGGC CTGGGCTCCCCGCTGCCCTCGCCCCGGGCCTCCCCTCGGCCATGGACCCCCGAAGATCCCTGGAGCCTGT ATGGTCCAAGCCCCGGAGGCCGAGGGCCAGAGGATAGCTGGCTACTCCTCAGTGCTCCTGGGCCCACCCC AGCCTCCCCGCGGCCTGCCTCTCCATGTGGCAAGCGGCGCTATTCCAGCTCGGGAACCCCATCTTCAGCC TCCCCAGCTCTGTCCCGCCGTGGCAGCCTGGGGGAAGAGGGGTCTGAGCCACCTCCACCACCCCCATTGC CTCTGGCCCGGGACCCGGGCTCCCCTGGTCCCTTTGACTATGTGGGGGCCCCACCAGCTGAGAGCATCCC TCAGAAGACACGGCGGACTTCCAGCGAGCAGGCAGTGGCTCTGCCTCGGTCTGAGGAGCCTGCCTCATGC AATGGGAAGCTGCCCTTGGGAGCAGAGGAGTCTGTGGCTCCTCCAGGAGGTTCCCGGAAGGAGGTGGCTG GCATGGACTACCTGGCAGTGCCCTCCCCACTCGCTTGGTCCAAGGCCCGGATTGGGGGACACAGCCCTAT CTTCAGGACCTCTGCCCTACCCCCACTGGACTGGCCTCTGCCCAGCCAATATGAGCAGCTGGAGCTGAGG ATCGAGGTACAGCCTAGAGCCCACCACCGGGCCCACTATGAGACAGAAGGCAGCCGTGGAGCTGTCAAAG CTGCCCCTGGCGGTCACCCCGTAGTCAAGCTCCTAGGCTACAGTGAGAAGCCACTGACCCTACAGATGTT CATCGGCACTGCAGATGAAAGGAACCTGCGGCCTCATGCCTTCTATCAGGTGCACCGTATCACAGGCAAG ATGGTGGCCACGGCCAGCTATGAAGCCGTAGTCAGTGGCACCAAGGTGTTGGAGATGACTCTGCTGCCTG AGAACAACATGGCGGCCAACATTGACTGCGCGGGAATCCTGAAGCTTCGGAATTCAGACATTGAGCTTCG GAAGGGTGAGACGGACATCGGGCGCAAAAACACACGTGTACGGCTGGTGTTCCGGGTACACGTGCCCCAG GGCGGCGGGAAGGTCGTCTCAGTACAGGCAGCATCGGTGCCCATCGAGTGCTCCCAGCGCTCAGCCCAGG AGCTGCCCCAGGTGGAGGCCTACAGCCCCAGTGCCTGCTCTGTGAGAGGAGGCGAGGAACTGGTACTGAC TGGCTCCAACTTCCTGCCAGACTCCAAGGTGGTGTTCATTGAGAGGGGTCCTGATGGGAAGCTGCAATGG GAGGAGGAGGCCACAGTGAACCGACTGCAGAGCAACGAGGTGACGCTGACCCTGACTGTCCCCGAGTACA GCAACAAGAGGGTTTCCCGGCCAGTCCAGGTCTACTTTTATGTCTCCAATGGGCGGAGGAAACGCAGTCC TACCCAGAGTTTCAGGTTTCTGCCTGTGATCTGCAAAGAGGAGCCCCTACCGGACTCATCTCTGCGGGGT TTCCCTTCAGCATCGGCAACCCCCTTTGGCACTGACATGGACTTCTCACCACCCAGGCCCCCCTACCCCT CCTATCCCCATGAAGACCCTGCTTGCGAAACTCCTTACCTATCAGAAGGCTTCGGCTATGGCATGCCCCC TCTGTACCCCCAGACGGGGCCCCCACCATCCTACAGACCGGGCCTGCGGATGTTCCCTGAGACTAGGGGT ACCACAGGTTGTGCCCAACCACCTGCAGTTTCCTTCCTTCCCCGCCCCTTCCCTAGTGACCCGTATGGAG GGCGGGGCTCCTCTTTCTCCCTGGGGCTGCCATTCTCTCCGCCAGCCCCCTTTCGGCCGCCTCCTCTTCC TGCATCCCCACCGCTTGAAGGCCCCTTCCCTTCCCAGAGTGATGTGCATCCCCTACCTGCTGAGGGATAC AATAAGGTAGGGCCAGGCTATGGCCCTGGGGAGGGGGCTCCGGAGCAGGAGAAATCCAGGGGTGGCTACA GCAGCGGCTTCCGAGACAGTGTCCCTATCCAGGGTATCACGCTGGAGGAAGTGAGTGAGATCATTGGCCG AGACCTGAGTGGCTTCCCTGCACCTCCTGGAGAAGAGCCTCCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230880 representing NM_001198966
Red=Cloning site Green=Tags(s) MHSPPPRPAPSPGTWESQPARSVRLGGPGGGAGGAGGGRVLECPSIRITSISPTPEPPAALEDNPDAWGD GSPRDYPPPEGFGGYREAGGQGGGAFFSPSPGSSSLSSWSFFSDASDEAALYAACDEVESELNEAASRFG LGSPLPSPRASPRPWTPEDPWSLYGPSPGGRGPEDSWLLLSAPGPTPASPRPASPCGKRRYSSSGTPSSA SPALSRRGSLGEEGSEPPPPPPLPLARDPGSPGPFDYVGAPPAESIPQKTRRTSSEQAVALPRSEEPASC NGKLPLGAEESVAPPGGSRKEVAGMDYLAVPSPLAWSKARIGGHSPIFRTSALPPLDWPLPSQYEQLELR IEVQPRAHHRAHYETEGSRGAVKAAPGGHPVVKLLGYSEKPLTLQMFIGTADERNLRPHAFYQVHRITGK MVATASYEAVVSGTKVLEMTLLPENNMAANIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHVPQ GGGKVVSVQAASVPIECSQRSAQELPQVEAYSPSACSVRGGEELVLTGSNFLPDSKVVFIERGPDGKLQW EEEATVNRLQSNEVTLTLTVPEYSNKRVSRPVQVYFYVSNGRRKRSPTQSFRFLPVICKEEPLPDSSLRG FPSASATPFGTDMDFSPPRPPYPSYPHEDPACETPYLSEGFGYGMPPLYPQTGPPPSYRPGLRMFPETRG TTGCAQPPAVSFLPRPFPSDPYGGRGSSFSLGLPFSPPAPFRPPPLPASPPLEGPFPSQSDVHPLPAEGY NKVGPGYGPGEGAPEQEKSRGGYSSGFRDSVPIQGITLEEVSEIIGRDLSGFPAPPGEEPPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001198966 |
| ORF Size | 2496 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001198966.2, NP_001185895.1 |
| RefSeq Size | 4630 bp |
| RefSeq ORF | 2499 bp |
| Locus ID | 4776 |
| UniProt ID | Q14934 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Axon guidance, B cell receptor signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway |
| MW | 88.3 kDa |
| Gene Summary | This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230880L3 | Lenti-ORF clone of NFATC4 (Myc-DDK-tagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (NFATC4), transcript variant 4 |
CNY 10,170.00 |
|
| RC230880L4 | Lenti-ORF clone of NFATC4 (mGFP-tagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (NFATC4), transcript variant 4 |
CNY 10,170.00 |
|
| RG230880 | NFATC4 (tGFP-tagged) - Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (NFATC4), transcript variant 4 |
CNY 9,120.00 |
