LTBP3 (NM_001164266) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DASS; GPHYSD3; LTBP-3; LTBP2; pp6425; STHAG6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC229066 representing NM_001164266 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGCGGCCAGTGCTCCTCGCGAAACCAGTGCCTGTGTCCCCCGGACTTCACTGGGCGCTTCTGCC AGGTGCCCGCAGGAGGAGCCGGTGGGGGTACCGGCGGCTCAGGCCCCGGCCTGAGCAGGACAGGGGCCCT GTCCACAGGGGCGCTGCCGCCCCTGGCTCCGGAGGGCGACTCTGTGGCCAGCAAGCACGCCATCTACGCC GTCCAGGTGATCGCTGACCCTCCTGGGCCCGGGGAGGGGCCTCCTGCCCAGCACGCAGCCTTCCTGGTGC CCCTAGGCCCGGGACAGATCTCAGCAGAAGTGCAGGCCCCGCCCCCCGTGGTGAATGTGCGCGTCCATCA CCCGCCCGAGGCCTCAGTCCAGGTGCACCGCATTGAGAGCTCGAACGCCGAGAGCGCAGCCCCCTCCCAG CACCTGCTGCCGCACCCCAAGCCCTCGCACCCCCGGCCGCCCACCCAGAAGCCCCTGGGCCGCTGCTTTC AGGACACTCTGCCCAAGCAGCCGTGTGGCAGCAACCCCCTCCCCGGCCTCACCAAGCAGGAAGACTGCTG CGGTAGCATCGGCACTGCCTGGGGCCAGAGCAAGTGCCACAAGTGTCCCCAGCTGCAGTACACAGGAGTG CAGAAGCCAGGGCCTGTACGTGGGGAAGTGGGCGCTGACTGTCCCCAGGGCTACAAGAGGCTTAACAGCA CCCACTGCCAGGACATCAACGAGTGCGCAATGCCGGGCGTGTGTCGCCATGGTGACTGCCTCAACAACCC TGGCTCCTATCGCTGTGTCTGCCCACCTGGCCATAGTTTAGGCCCCTCCCGTACACAGTGCATTGCAGAC AAACCGGAGGAGAAGAGCCTGTGTTTCCGCCTGGTGAGCCCTGAGCACCAGTGCCAGCACCCACTGACCA CCCGCCTGACCCGCCAGCTCTGCTGCTGCAGTGTCGGCAAGGCCTGGGGCGCGCGGTGTCAGCGCTGCCC AACAGATGGCACCGCTGCGTTCAAGGAGATCTGCCCAGCTGGGAAGGGATACCACATTCTCACCTCCCAC CAGACGCTCACCATTCAGGGCGAGAGTGACTTTTCCCTTTTCCTGCACCCTGACGGGCCACCCAAGCCCC AGCAGCTTCCGGAGAGCCCTAGCCAGGCTCCACCACCTGAGGACACAGAGGAAGAGAGAGGGGTGACCAC GGACTCACCGGTGAGTGAGGAGAGGTCAGTGCAGCAGAGCCACCCAACTGCCACCACGACTCCTGCCCGG CCCTACCCCGAGCTGATCTCCCGTCCCTCGCCCCCGACCATGCGCTGGTTCCTGCCGGACTTGCCTCCTT CCCGCAGCGCCGTAGAGATCGCTCCCACTCAGGTCACAGAGACTGATGAGTGCCGACTGAACCAGAACAT CTGTGGCCACGGAGAGTGCGTGCCGGGCCCCCCTGACTACTCCTGCCACTGCAACCCCGGCTACCGGTCA CATCCCCAGCACCGCTACTGCGTGGATGTGAACGAGTGCGAGGCAGAGCCCTGTGGCCCGGGGAGGGGCA TCTGCATGAACACCGGCGGCTCCTACAATTGCCACTGCAACCGCGGCTACCGCCTGCACGTGGGCGCCGG GGGGCGCTCGTGCGTGGACCTGAACGAATGCGCCAAGCCCCACCTGTGCGGCGACGGCGGCTTCTGCATC AACTTTCCCGGTCACTACAAGTGCAACTGCTACCCCGGCTACCGGCTCAAAGCCTCCCGGCCTCCTGTGT GCGAAGACATCGACGAGTGCCGGGACCCAAGCTCTTGCCCGGATGGCAAATGCGAGAACAAGCCCGGGAG CTTCAAGTGCATCGCCTGTCAGCCTGGCTACCGCAGCCAGGGGGGCGGGGCCTGTCGCGACGTGAACGAG TGCGCCGAGGGCAGCCCCTGCTCGCCTGGCTGGTGCGAGAACCTCCCGGGCTCCTTCCGCTGCACCTGTG CCCAGGGCTACGCGCCCGCGCCCGACGGCCGCAGTTGCTTGGATGTGGACGAGTGTGAGGCTGGGGACGT GTGTGACAATGGCATCTGCAGCAACACGCCAGGATCTTTCCAGTGTCAGTGCCTCTCTGGCTACCATCTG TCCAGGGACCGGAGCCACTGCGAGGACATTGATGAGTGTGACTTCCCTGCAGCCTGCATTGGGGGTGACT GCATCAATACCAATGGCTCCTACAGATGTCTTTGCCCCCAGGGGCATCGGCTGGTGGGTGGCAGGAAATG CCAAGACATAGATGAGTGCAGCCAGGACCCGAGCCTGTGCCTTCCCCATGGGGCCTGCAAGAACCTTCAG GGCTCCTATGTGTGTGTCTGCGATGAGGGCTTCACTCCCACCCAGGACCAGCACGGTTGTGAGGAGGTGG AGCAGCCCCACCACAAGAAGGAGTGCTACCTGAACTTCGATGACACAGTGTTCTGCGACAGCGTATTGGC CACCAACGTGACCCAGCAGGAGTGCTGCTGCTCTCTGGGGGCCGGCTGGGGCGACCACTGCGAAATCTAC CCCTGCCCAGTCTACAGCTCAGCCGAGTTCCACAGCCTCTGCCCAGACGGAAAGGGCTACACCCAGGACA ACAACATCGTCAACTACGGCATCCCAGCCCACCGTGACATCGACGAGTGCATGTTGTTCGGGTCGGAGAT TTGCAAGGAGGGCAAGTGCGTGAACACGCAGCCTGGCTACGAGTGCTACTGCAAGCAGGGCTTCTACTAC GACGGGAACCTGCTGGAATGCGTGGACGTGGACGAGTGCCTGGACGAGTCCAACTGCCGGAACGGAGTGT GTGAGAACACGCGCGGCGGCTACCGCTGTGCCTGCACGCCCCCTGCCGAGTACAGTCCCGCGCAGCGCCA GTGCCTGAGCCCGGAAGAGATGGAGCGTGCCCCGGAGCGGCGCGACGTGTGCTGGAGCCAGCGCGGAGAG GACGGCATGTGCGCTGGCCCCCTGGCCGGGCCTGCCCTCACCTTCGACGACTGCTGCTGCCGCCAGGGCC GCGGCTGGGGCGCCCAATGCCGACCGTGCCCGCCGCGCGGCGCGGGGTCCCATTGCCCGACATCGCAGAG CGAGAGCAATTCCTTCTGGGACACAAGCCCCCTGCTGTTGGGGAAGCCCCCAAGAGATGAGGACAGTTCA GAGGAGGATTCAGACGAGTGTCGCTGCGTGAGTGGCCGCTGCGTGCCGCGGCCGGGCGGCGCCGTGTGCG AGTGTCCCGGCGGCTTCCAGCTCGACGCCTCCCGCGCCCGCTGCGTGGATATCGACGAGTGCCGAGAGCT GAACCAGCGCGGGCTGCTGTGCAAGAGCGAGCGCTGCGTGAACACCAGCGGCTCCTTCCGCTGCGTCTGC AAAGCCGGCTTCGCGCGCAGCCGCCCGCACGGGGCCTGCGTTCCCCAGCGCCGCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC229066 representing NM_001164266 Red=Cloning site Green=Tags(s) MNGGQCSSRNQCLCPPDFTGRFCQVPAGGAGGGTGGSGPGLSRTGALSTGALPPLAPEGDSVASKHAIYA VQVIADPPGPGEGPPAQHAAFLVPLGPGQISAEVQAPPPVVNVRVHHPPEASVQVHRIESSNAESAAPSQ HLLPHPKPSHPRPPTQKPLGRCFQDTLPKQPCGSNPLPGLTKQEDCCGSIGTAWGQSKCHKCPQLQYTGV QKPGPVRGEVGADCPQGYKRLNSTHCQDINECAMPGVCRHGDCLNNPGSYRCVCPPGHSLGPSRTQCIAD KPEEKSLCFRLVSPEHQCQHPLTTRLTRQLCCCSVGKAWGARCQRCPTDGTAAFKEICPAGKGYHILTSH QTLTIQGESDFSLFLHPDGPPKPQQLPESPSQAPPPEDTEEERGVTTDSPVSEERSVQQSHPTATTTPAR PYPELISRPSPPTMRWFLPDLPPSRSAVEIAPTQVTETDECRLNQNICGHGECVPGPPDYSCHCNPGYRS HPQHRYCVDVNECEAEPCGPGRGICMNTGGSYNCHCNRGYRLHVGAGGRSCVDLNECAKPHLCGDGGFCI NFPGHYKCNCYPGYRLKASRPPVCEDIDECRDPSSCPDGKCENKPGSFKCIACQPGYRSQGGGACRDVNE CAEGSPCSPGWCENLPGSFRCTCAQGYAPAPDGRSCLDVDECEAGDVCDNGICSNTPGSFQCQCLSGYHL SRDRSHCEDIDECDFPAACIGGDCINTNGSYRCLCPQGHRLVGGRKCQDIDECSQDPSLCLPHGACKNLQ GSYVCVCDEGFTPTQDQHGCEEVEQPHHKKECYLNFDDTVFCDSVLATNVTQQECCCSLGAGWGDHCEIY PCPVYSSAEFHSLCPDGKGYTQDNNIVNYGIPAHRDIDECMLFGSEICKEGKCVNTQPGYECYCKQGFYY DGNLLECVDVDECLDESNCRNGVCENTRGGYRCACTPPAEYSPAQRQCLSPEEMERAPERRDVCWSQRGE DGMCAGPLAGPALTFDDCCCRQGRGWGAQCRPCPPRGAGSHCPTSQSESNSFWDTSPLLLGKPPRDEDSS EEDSDECRCVSGRCVPRPGGAVCECPGGFQLDASRARCVDIDECRELNQRGLLCKSERCVNTSGSFRCVC KAGFARSRPHGACVPQRRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001164266
ORF Size	3417 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001164266.1, NP_001157738.1
RefSeq ORF	3420 bp
Locus ID	4054
UniProt ID	Q9NS15
Protein Families	Transmembrane
MW	122.4 kDa
Gene Summary	The protein encoded by this gene forms a complex with transforming growth factor beta (TGF-beta) proteins and may be involved in their subcellular localization. Activation of this complex requires removal of the encoded binding protein. This protein also may play a structural role in the extracellular matrix. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

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