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OGDHL (NM_001143996) Human Tagged ORF Clone
CAT＃: RC227996
- TrueORF®
OGDHL (Myc-DDK-tagged)-Human oxoglutarate dehydrogenase-like (OGDHL), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: tGFP
"NM_001143996" in other vectors (4)
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CNY 9,790.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227996 representing NM_001143996
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCAGCTGAGGCTGCTGCCGTCCCGTCTTGGGGTACAGGCTGCGAGGCTCCTGGCTGCACATGACG TCCCGGTGTTTGGCTGGCGCAGCAGGTCCTCCGGGCCACCGGCCACCTTCCCAAGCAGCAAAGGTGGAGG CGGCTCCAGTTACATGGAGGAGATGTACTTCGCCTGGTTGGAAAACCCCCAGAGTGTCCACAAGATCCGG GGTCACCATGTGGCCCAGCTGGACCCCCTGGGCATTCTGGATGCAGACCTGGACTCCTTTGTGCCCTCAG ACTTGATCACAACCATTGATAAACTGGCCTTCTATGACCTTCAGGAGGCTGACCTTGATAAGGAGTTCCA GCTGCCGACAACCACCTTCATTGGGGGCTCTGAAAACACCCTCTCTCTGCGGGAGATCATTCGGCGCCTG GAGAACACCTACTGCCAGCACATTGGCCTGGAGTTCATGTTCATCAACGATGTGGAGCAGTGCCAGTGGA TCCGGCAGAAGTTTGAGACCCCTGGTGTGATGCAGTTCTCCAGCGAGGAGAAGCGGACCCTGCTGGCCCG GCTAGTGCGCTCCATGAGGTTTGAAGACTTCCTGGCCCGGAAATGGTCCTCAGAGAAGCGGTTTGGCCTG GAGGGCTGTGAAGTGATGATTCCTGCCCTCAAGACCATCATCGACAAATCCAGCGAGATGGGGATTGAGA ATGTCATCTTGGGGATGCCACACAGGGGAAGGCTGAACGTGCTGGCCAACGTGATCCGCAAGGACCTGGA GCAGATCTTCTGCCAGTTTGACCCCAAGCTGGAGGCGGCGGACGAGGGCTCCGGGGATGTCAAGTACCAC CTGGGCATGTACCATGAGAGGATCAACCGCGTCACCAACCGGAACATCACTCTGTCGCTGGTTGCCAACC CCTCCCACCTGGAGGCAGTGGACCCTGTGGTGCAGGGGAAGACAAAGGCAGAGCAGTTCTACCGTGGAGA TGCCCAGGGCAAGAAGGTCATGTCCATCCTGGTTCATGGGGACGCCGCCTTTGCTGGCCAGGGCGTGGTA TATGAGACCTTCCACCTGAGCGACCTGCCCTCCTACACGACCAATGGTACCGTGCACGTCGTCGTCAACA ACCAGATTGGATTCACCACAGACCCCCGAATGGCCCGCTCCTCACCATACCCGACCGACGTGGCCCGGGT GGTCAATGCGCCTATCTTCCATGTGAATGCCGATGACCCAGAGGCTGTGATATATGTGTGCAGTGTGGCA GCCGAATGGAGAAACACTTTCAACAAAGATGTTGTCGTGGACCTGGTCTGTTACCGCCGGCGTGGCCACA ATGAGATGGACGAGCCCATGTTCACCCAGCCGCTCATGTACAAGCAGATCCACAGACAGGTGCCTGTGCT GAAGAAGTACGCAGACAAGCTGATTGCCGAGGGCACAGTCACCCTGCAGGAGTTTGAGGAAGAAATTGCC AAATACGACCGGATCTGTGAGGAGGCTTATGGCAGGTCCAAGGATAAAAAGATTCTGCATATAAAGCACT GGTTGGACTCCCCCTGGCCTGGCTTCTTCAACGTAGATGGGGAGCCCAAGAGCATGACATGCCCAGCCAC GGGGATCCCTGAGGACATGCTCACCCACATCGGCAGTGTGGCCAGCTCTGTGCCCCTGGAGGACTTTAAG ATCCACACTGGCCTCTCTCGCATTCTGCGGGGCCGTGCGGACATGACCAAGAACCGGACGGTGGACTGGG CGTTGGCAGAGTACATGGCCTTTGGCTCCCTGCTGAAGGAAGGCATCCACGTGCGGCTCAGCGGGCAGGA TGTGGAGAGGGGCACATTCAGTCACCGGCACCATGTTCTCCATGACCAGGAGGTTGACCGCAGGACGTGT GTGCCTATGAATCATCTCTGGCCTGACCAGGCCCCGTACACCGTGTGCAACAGCTCCCTCTCGGAGTACG GAGTCCTGGGCTTTGAGCTGGGCTATGCCATGGCCAGCCCCAATGCCCTGGTCCTCTGGGAGGCCCAGTT TGGGGACTTCCACAACACGGCCCAGTGCATCATCGACCAGTTCATCAGCACCGGCCAGGCCAAGTGGGTG CGGCATAATGGCATTGTGCTGCTGCTGCCCCATGGCATGGAAGGCATGGGCCCAGAGCACTCGTCAGCGA GGCCCGAAAGGTTCCTGCAGATGAGCAATGATGACTCGGATGCCTACCCTGCATTCACCAAGGACTTCGA GGTGAGCCAGCTCTATGACTGCAACTGGATCGTGGTCAACTGCTCCACACCGGCCAACTACTTCCACGTG CTGCGCCGGCAGATCCTGCTGCCCTTCCGCAAGCCGCTGATTATCTTCACACCTAAATCTCTGCTGAGGC ACCCAGAGGCCAAGTCCAGCTTTGACCAAATGGTATCCGGGACCAGCTTCCAGCGGGTGATTCCTGAAGA TGGGGCCGCAGCACGGGCCCCTGAGCAGGTGCAGCGGCTCATCTTCTGCACGGGAAAGGTGTACTATGAC CTGGTGAAGGAGCGGAGCAGCCAGGACCTGGAGGAGAAAGTGGCCATCACGCGCCTGGAGCAGATCTCTC CATTCCCCTTCGACCTGATCAAGCAGGAGGCAGAGAAGTACCCAGGTGCGGAGCTGGCCTGGTGTCAGGA GGAGCACAAGAACATGGGCTACTATGACTACATCAGCCCACGCTTCATGACCATCCTGAGGCGCGCACGG CCCATATGGTATGTTGGCCGGGACCCAGCGGCTGCACCAGCCACAGGAAACAGGAACACTCACCTGGTGT CACTGAAGAAGTTTCTGGATACTGCCTTCAATCTCCAGGCCTTTGAGGGCAAGACATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227996 representing NM_001143996
Red=Cloning site Green=Tags(s) MSQLRLLPSRLGVQAARLLAAHDVPVFGWRSRSSGPPATFPSSKGGGGSSYMEEMYFAWLENPQSVHKIR GHHVAQLDPLGILDADLDSFVPSDLITTIDKLAFYDLQEADLDKEFQLPTTTFIGGSENTLSLREIIRRL ENTYCQHIGLEFMFINDVEQCQWIRQKFETPGVMQFSSEEKRTLLARLVRSMRFEDFLARKWSSEKRFGL EGCEVMIPALKTIIDKSSEMGIENVILGMPHRGRLNVLANVIRKDLEQIFCQFDPKLEAADEGSGDVKYH LGMYHERINRVTNRNITLSLVANPSHLEAVDPVVQGKTKAEQFYRGDAQGKKVMSILVHGDAAFAGQGVV YETFHLSDLPSYTTNGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNADDPEAVIYVCSVA AEWRNTFNKDVVVDLVCYRRRGHNEMDEPMFTQPLMYKQIHRQVPVLKKYADKLIAEGTVTLQEFEEEIA KYDRICEEAYGRSKDKKILHIKHWLDSPWPGFFNVDGEPKSMTCPATGIPEDMLTHIGSVASSVPLEDFK IHTGLSRILRGRADMTKNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQEVDRRTC VPMNHLWPDQAPYTVCNSSLSEYGVLGFELGYAMASPNALVLWEAQFGDFHNTAQCIIDQFISTGQAKWV RHNGIVLLLPHGMEGMGPEHSSARPERFLQMSNDDSDAYPAFTKDFEVSQLYDCNWIVVNCSTPANYFHV LRRQILLPFRKPLIIFTPKSLLRHPEAKSSFDQMVSGTSFQRVIPEDGAAARAPEQVQRLIFCTGKVYYD LVKERSSQDLEEKVAITRLEQISPFPFDLIKQEAEKYPGAELAWCQEEHKNMGYYDYISPRFMTILRRAR PIWYVGRDPAAAPATGNRNTHLVSLKKFLDTAFNLQAFEGKTF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001143996 |
| ORF Size | 2859 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001143996.1, NP_001137468.1 |
| RefSeq ORF | 2862 bp |
| Locus ID | 55753 |
| UniProt ID | Q9ULD0 |
| Protein Pathways | Citrate cycle (TCA cycle), Lysine degradation, Metabolic pathways, Tryptophan metabolism |
| MW | 108 kDa |
| Gene Summary | The protein encoded by this gene is similar to oxoglutarate dehydrogenase (OGDH) of the OGDH complex, which degrades glucose and glutamate. This gene encodes several isoforms, including some that appear to localize to mitochondria. The encoded protein down-regulates the AKT signaling cascade and can suppress the growth of cervical cancer cells. [provided by RefSeq, Dec 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227996L3 | Lenti-ORF clone of OGDHL (Myc-DDK-tagged)-Human oxoglutarate dehydrogenase-like (OGDHL), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 11,690.00 |
|
| RC227996L4 | Lenti-ORF clone of OGDHL (mGFP-tagged)-Human oxoglutarate dehydrogenase-like (OGDHL), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 11,690.00 |
|
| RG227996 | OGDHL (tGFP-tagged) - Human oxoglutarate dehydrogenase-like (OGDHL), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 10,740.00 |
|
| SC326298 | OGDHL (untagged)-Human oxoglutarate dehydrogenase-like (OGDHL), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 18,430.00 |
