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ARHGAP32 (NM_001142685) Human Tagged ORF Clone
CAT＃: RC227921
- TrueORF®
ARHGAP32 (Myc-DDK-tagged)-Human Rho GTPase activating protein 32 (ARHGAP32), transcript variant 1
ORF Plasmid: tGFP
"NM_001142685" in other vectors (3)
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CNY 20,664.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS; RICS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC227921 representing NM_001142685
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTGAAAGTGAGAGTAGCACTTTAGGGGATGACAGTGTCTTCTGGTTGGAGTCTGAAGTTATAA TCCAGGTGACTGACTGTGAAGAGGAAGAAAGGGAAGAGAAGTTCAGGAAGATGAAGTCCTCAGTACATTC TGAAGAAGATGATTTTGTTCCAGAGCTACATAGAAATGTACACCCTCGAGAGCGGCCTGATTGGGAAGAA ACTCTTAGCGCAATGGCAAGAGGCGCAGATGTTCCAGAGATTCCTGGAGATCTTACTCTTAAGACGTGTG GCAGTACAGCCAGTATGAAGGTTAAGCATGTGAAAAAGTTACCCTTCACTAAAGGTCACTTTCCGAAGAT GGCTGAATGTGCGCATTTCCATTATGAGAATGTTGAGTTCGGCAGCATACAGCTTTCACTTTCAGAAGAA CAGAATGAAGTAATGAAAAATGGCTGTGAATCTAAAGAGCTGGTCTACCTCGTGCAGATTGCTTGTCAGG GAAAAAGTTGGATTGTTAAAAGAAGTTATGAAGATTTTCGGGTACTTGATAAACATCTTCATCTGTGTAT TTATGACCGAAGATTTTCCCAGCTCTCAGAACTTCCCCGTTCTGACACCCTGAAGGACAGTCCAGAGTCG GTCACTCAGATGCTTATGGCTTACCTGTCACGCCTTTCAGCTATCGCTGGCAACAAGATCAACTGTGGGC CCGCCCTTACCTGGATGGAGATTGATAATAAGGGAAATCACCTTTTGGTTCATGAGGAGTCATCCATCAA CACTCCTGCTGTCGGTGCTGCCCATGTTATCAAGAGGTACACTGCTCGGGCCCCTGACGAACTGACCTTA GAGGTGGGAGACATTGTTTCTGTTATTGACATGCCCCCGAAAGTGTTAAGCACATGGTGGAGAGGCAAGC ACGGATTCCAGGTGGGACTCTTCCCTGGACACTGTGTTGAGTTAATTAACCAAAAAGTTCCCCAGTCAGT GACCAACTCAGTGCCAAAACCAGTGTCTAAAAAGCACGGCAAGCTCATTACGTTCTTACGAACATTCATG AAGTCTCGTCCAACAAAACAGAAGCTGAAGCAGCGGGGAATCTTGAAAGAGAGGGTGTTTGGTTGTGACC TGGGGGAACACCTTCTAAATTCTGGTTTTGAAGTGCCGCAGGTTCTTCAAAGCTGCACAGCATTCATTGA GAGATATGGCATCGTGGATGGAATCTATCGCCTTTCTGGTGTTGCCTCCAATATCCAGAGACTACGCCAT GAATTTGACTCTGAGCACGTCCCCGACCTGACGAAAGAACCGTATGTTCAGGACATCCATTCTGTGGGTT CCCTATGTAAGCTGTACTTCCGGGAACTCCCAAACCCTCTGCTTACCTACCAGCTGTATGAGAAATTTTC TGATGCAGTTTCAGCAGCAACAGATGAAGAAAGGCTGATAAAAATCCACGATGTCATCCAGCAGCTCCCC CCACCACACTACAGAACACTGGAGTTCCTGATGAGACACTTGTCTCTTCTAGCTGACTATTGTTCCATCA CAAATATGCATGCAAAAAATCTAGCAATTGTTTGGGCTCCAAACCTGTTAAGATCAAAACAGATAGAATC TGCCTGCTTCAGTGGAACAGCAGCTTTCATGGAAGTGAGGATTCAGTCTGTGGTTGTTGAGTTCATCCTG AATCACGTTGATGTGCTGTTCAGCGGCAGAATCAGCATGGCCATGCAAGAGGGGGCAGCTTCTCTATCAA GGCCCAAGTCCCTCCTGGTATCCTCTCCATCCACCAAACTGCTGACATTGGAAGAGGCCCAGGCACGAAC ACAAGCTCAGGTCAATTCTCCAATTGTGACGGAAAATAAATATATCGAAGTAGGAGAAGGACCTGCTGCA CTTCAGGGGAAATTTCATACCATAATTGAGTTCCCACTTGAAAGAAAGAGGCCTCAAAATAAGATGAAAA AGTCTCCTGTGGGTAGCTGGCGTTCCTTTTTCAACTTGGGGAAATCATCATCTGTTTCTAAACGAAAGCT GCAGCGGAATGAGAGTGAGCCTTCAGAGATGAAAGCCATGGCTCTGAAAGGTGGCAGGGCAGAAGGAACC CTCCGTTCAGCTAAAAGTGAGGAGTCTCTTACATCTCTCCATGCAGTTGATGGTGATTCTAAGCTCTTCC GACCCAGAAGACCCAGATCTTCCAGTGATGCACTGTCTGCCTCTTTTAATGGAGAAATGCTGGGGAACCG CTGTAACTCCTATGATAATCTGCCTCATGACAATGAGAGTGAGGAGGAAGGAGGGCTGCTTCATATCCCA GCCCTTATGTCTCCTCATTCAGCTGAGGATGTTGACTTGAGCCCACCAGACATTGGAGTAGCCAGCCTGG ATTTTGATCCAATGTCATTTCAATGTAGTCCTCCTAAGGCCGAATCAGAATGTCTGGAGAGTGGTGCTTC CTTTTTAGATTCACCAGGATACTCCAAGGATAAACCAAGTGCCAATAAAAAGGATGCAGAAACAGGTAGT AGCCAATGTCAGACTCCAGGAAGCACAGCAAGCTCTGAACCTGTCTCTCCTCTTCAGGAGAAACTGAGTC CATTCTTTACCCTGGACTTGAGCCCAACTGAAGATAAATCATCTAAGCCATCCTCCTTTACTGAAAAGGT CGTCTATGCTTTCTCTCCGAAGATAGGACGGAAATTAAGCAAATCACCTTCTATGAGCATATCTGAGCCA ATTTCAGTGACCCTACCACCACGGGTGTCAGAAGTCATTGGTACAGTCTCAAATACCACAGCTCAGAATG CATCATCTTCAACCTGGGACAAATGCGTTGAAGAAAGGGATGCCACAAATAGATCCCCCACCCAGATAGT AAAGATGAAAACAAATGAGACAGTTGCCCAAGAAGCATATGAATCTGAAGTCCAGCCCCTGGACCAGGTG GCTGCTGAAGAAGTAGAATTGCCAGGGAAAGAGGATCAGTCTGTCTCAAGCAGTCAGAGTAAGGCTGTAG CTTCTGGACAGACTCAGACAGGAGCAGTTACCCATGACCCCCCTCAGGATTCCGTTCCTGTCAGTTCAGT CTCTCTTATCCCACCACCACCGCCTCCGAAAAATGTTGCCCGAATGTTGGCGCTAGCATTAGCTGAGTCC GCACAGCAAGCCTCAACTCAGTCATTGAAGAGACCAGGGACCTCTCAGGCTGGGTATACAAATTATGGAG ACATAGCGGTGGCTACAACTGAAGATAATCTGTCCAGTTCTTACTCTGCAGTTGCTCTAGATAAGGCCTA TTTCCAAACCGATCGACCAGCAGAGCAGTTCCACCTCCAGAATAATGCACCAGGAAACTGTGACCATCCT CTACCAGAGACAACAGCTACTGGGGATCCTACCCATTCCAACACAACTGAATCTGGGGAGCAACATCACC AAGTAGACTTAACAGGGAATCAGCCACATCAAGCATATTTATCTGGGGACCCAGAAAAGGCCAGAATTAC TTCAGTTCCCTTAGACTCAGAGAAGTCTGATGATCATGTAAGTTTCCCTGAAGACCAGTCTGGGAAGAAC AGTATGCCAACTGTCTCCTTCTTGGATCAGGACCAGTCTCCACCCCGTTTCTACAGTGGAGATCAGCCTC CTTCTTATCTTGGTGCAAGTGTGGATAAACTCCATCACCCTTTAGAATTTGCAGACAAATCTCCCACACC TCCTAATTTACCTAGCGATAAAATCTACCCTCCTTCTGGGTCCCCCGAAGAGAATACCAGCACAGCCACC ATGACTTACATGACAACTACTCCAGCAACAGCCCAAATGAGCACCAAGGAAGCCAGCTGGGATGTGGCTG AACAACCCACCACTGCTGATTTTGCTGCTGCCACACTTCAGCGCACGCACAGAACTAATCGTCCCCTTCC CCCTCCGCCTTCCCAGAGATCTGCAGAGCAGCCACCAGTTGTGGGGCAGGTACAAGCAGCAACCAATATA GGATTAAATAATTCCCACAAGGTTCAAGGAGTAGTTCCAGTTCCAGAGAGGCCACCTGAACCTCGAGCCA TGGATGACCCTGCGTCTGCCTTCATCAGTGACAGTGGTGCTGCTGCTGCTCAGTGTCCCATGGCTACAGC TGTCCAGCCAGGCCTGCCTGAGAAAGTGCGGGACGGTGCCCGGGTCCCGCTGCTGCACCTGCGCGCCGAG TCTGTCCCTGCGCATCCCTGTGGCTTTCCTGCACCACTGCCCCCCACCAGGATGATGGAGAGTAAGATGA TTGCTGCCATACACTCCAGCAGTGCAGATGCCACCAGCAGTTCAAATTATCATTCCTTTGTCACTGCTTC ATCCACCTCTGTGGACGATGCATTGCCTTTACCACTTCCTGTCCCACAACCTAAGCATGCTTCTCAGAAA ACAGTTTACTCCTCCTTTGCTAGGCCCGATGTCACCACTGAACCCTTTGGTCCAGATAACTGTTTGCATT TCAATATGACTCCAAACTGCCAGTACCGTCCCCAGAGTGTACCTCCCCATCACAATAAATTGGAGCAGCA CCAAGTGTATGGTGCCAGGTCAGAGCCACCAGCCTCCATGGGTCTTCGTTATAACACATATGTGGCCCCA GGAAGAAACGCATCTGGACACCACTCCAAGCCATGCAGCCGGGTCGAGTATGTGTCTTCTTTGAGCTCCT CTGTCAGGAATACCTGTTACCCCGAAGACATTCCACCGTACCCTACCATCCGGAGAGTGCAGTCTCTCCA TGCTCCGCCGTCTTCCATGATTCGCTCTGTTCCCATTTCACGGACAGAAGTTCCCCCAGATGATGAGCCA GCCTACTGCCCAAGACCTCTGTACCAATATAAGCCATATCAGTCCTCCCAGGCCCGCTCAGATTATCATG TCACTCAGCTTCAGCCTTACTTTGAGAATGGCCGGGTCCACTACAGGTATAGCCCATATTCCAGTTCTTC TAGTTCCTATTACAGTCCAGATGGGGCCCTGTGTGATGTGGATGCCTATGGCACAGTCCAGTTGAGACCC CTTCACCGCCTTCCCAATCGAGACTTTGCTTTCTACAATCCTAGGCTGCAAGGAAAGAGCTTGTACAGTT ATGCTGGTTTGGCTCCACGTCCCCGGGCCAACGTGACTGGCTATTTCTCTCCCAACGACCATAATGTAGT CAGCATGCCTCCGGCTGCTGATGTGAAGCACACCTACACCTCATGGGATCTTGAGGACATGGAAAAATAC CGCATGCAGTCCATCCGGAGAGAGAGCCGTGCTCGGCAGAAGGTGAAAGGGCCTGTCATGTCCCAATATG ATAACATGACCCCGGCGGTGCAGGACGACTTGGGTGGGATCTATGTCATCCATCTGCGTAGTAAATCAGA TCCTGGGAAAACTGGACTTCTCTCAGTGGCAGAAGGAAAGGAGAGCCGCCATGCAGCCAAGGCCATCAGT CCCGAGGGAGAGGACCGCTTCTATAGGAGGCATCCCGAGGCAGAGATGGACAGAGCCCACCATCACGGAG GCCATGGTAGCACGCAGCCGGAGAAGCCATCCCTGCCTCAGAAGCAGAGCAGCCTGAGGAGCAGGAAGCT TCCTGACATGGGCTGCAGTCTTCCTGAGCACAGGGCACACCAAGAAGCAAGCCATAGGCAGTTCTGTGAG TCAAAGAATGGGCCCCCTTATCCCCAGGGAGCTGGCCAGTTAGATTATGGGTCCAAAGGGATTCCAGACA CTTCTGAGCCAGTCAGCTACCACAACTCTGGAGTAAAATATGCTGCATCCGGGCAAGAATCTTTAAGACT GAACCACAAAGAGGTAAGGCTCTCCAAAGAGATGGAGCGACCCTGGGTTAGGCAGCCTTCTGCCCCAGAG AAACACTCCAGAGACTGCTACAAGGAGGAAGAACACCTCACTCAGTCAATCGTCCCACCCCCTAAACCAG AGAGGAGTCATAGCCTCAAACTCCATCATACCCAGAACGTGGAGAGGGACCCCAGTGTGCTGTACCAGTA CCAACCACACGGCAAGCGCCAGAGCAGTGTGACTGTTGTGTCCCAGTATGATAACCTGGAAGATTACCAC TCCCTGCCTCAGCACCAGCGAGGAGTCTTTGGAGGGGGCGGCATGGGGACGTATGTGCCCCCTGGCTTTC CCCATCCACAGAGCAGGACCTATGCTACAGCGTTGGGTCAAGGGGCCTTCCTGCCCGCAGAGTTGTCCTT GCAGCATCCTGAAACACAGATCCATGCAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227921 representing NM_001142685
Red=Cloning site Green=Tags(s) METESESSTLGDDSVFWLESEVIIQVTDCEEEEREEKFRKMKSSVHSEEDDFVPELHRNVHPRERPDWEE TLSAMARGADVPEIPGDLTLKTCGSTASMKVKHVKKLPFTKGHFPKMAECAHFHYENVEFGSIQLSLSEE QNEVMKNGCESKELVYLVQIACQGKSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLSELPRSDTLKDSPES VTQMLMAYLSRLSAIAGNKINCGPALTWMEIDNKGNHLLVHEESSINTPAVGAAHVIKRYTARAPDELTL EVGDIVSVIDMPPKVLSTWWRGKHGFQVGLFPGHCVELINQKVPQSVTNSVPKPVSKKHGKLITFLRTFM KSRPTKQKLKQRGILKERVFGCDLGEHLLNSGFEVPQVLQSCTAFIERYGIVDGIYRLSGVASNIQRLRH EFDSEHVPDLTKEPYVQDIHSVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLIKIHDVIQQLP PPHYRTLEFLMRHLSLLADYCSITNMHAKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFIL NHVDVLFSGRISMAMQEGAASLSRPKSLLVSSPSTKLLTLEEAQARTQAQVNSPIVTENKYIEVGEGPAA LQGKFHTIIEFPLERKRPQNKMKKSPVGSWRSFFNLGKSSSVSKRKLQRNESEPSEMKAMALKGGRAEGT LRSAKSEESLTSLHAVDGDSKLFRPRRPRSSSDALSASFNGEMLGNRCNSYDNLPHDNESEEEGGLLHIP ALMSPHSAEDVDLSPPDIGVASLDFDPMSFQCSPPKAESECLESGASFLDSPGYSKDKPSANKKDAETGS SQCQTPGSTASSEPVSPLQEKLSPFFTLDLSPTEDKSSKPSSFTEKVVYAFSPKIGRKLSKSPSMSISEP ISVTLPPRVSEVIGTVSNTTAQNASSSTWDKCVEERDATNRSPTQIVKMKTNETVAQEAYESEVQPLDQV AAEEVELPGKEDQSVSSSQSKAVASGQTQTGAVTHDPPQDSVPVSSVSLIPPPPPPKNVARMLALALAES AQQASTQSLKRPGTSQAGYTNYGDIAVATTEDNLSSSYSAVALDKAYFQTDRPAEQFHLQNNAPGNCDHP LPETTATGDPTHSNTTESGEQHHQVDLTGNQPHQAYLSGDPEKARITSVPLDSEKSDDHVSFPEDQSGKN SMPTVSFLDQDQSPPRFYSGDQPPSYLGASVDKLHHPLEFADKSPTPPNLPSDKIYPPSGSPEENTSTAT MTYMTTTPATAQMSTKEASWDVAEQPTTADFAAATLQRTHRTNRPLPPPPSQRSAEQPPVVGQVQAATNI GLNNSHKVQGVVPVPERPPEPRAMDDPASAFISDSGAAAAQCPMATAVQPGLPEKVRDGARVPLLHLRAE SVPAHPCGFPAPLPPTRMMESKMIAAIHSSSADATSSSNYHSFVTASSTSVDDALPLPLPVPQPKHASQK TVYSSFARPDVTTEPFGPDNCLHFNMTPNCQYRPQSVPPHHNKLEQHQVYGARSEPPASMGLRYNTYVAP GRNASGHHSKPCSRVEYVSSLSSSVRNTCYPEDIPPYPTIRRVQSLHAPPSSMIRSVPISRTEVPPDDEP AYCPRPLYQYKPYQSSQARSDYHVTQLQPYFENGRVHYRYSPYSSSSSSYYSPDGALCDVDAYGTVQLRP LHRLPNRDFAFYNPRLQGKSLYSYAGLAPRPRANVTGYFSPNDHNVVSMPPAADVKHTYTSWDLEDMEKY RMQSIRRESRARQKVKGPVMSQYDNMTPAVQDDLGGIYVIHLRSKSDPGKTGLLSVAEGKESRHAAKAIS PEGEDRFYRRHPEAEMDRAHHHGGHGSTQPEKPSLPQKQSSLRSRKLPDMGCSLPEHRAHQEASHRQFCE SKNGPPYPQGAGQLDYGSKGIPDTSEPVSYHNSGVKYAASGQESLRLNHKEVRLSKEMERPWVRQPSAPE KHSRDCYKEEEHLTQSIVPPPKPERSHSLKLHHTQNVERDPSVLYQYQPHGKRQSSVTVVSQYDNLEDYH SLPQHQRGVFGGGGMGTYVPPGFPHPQSRTYATALGQGAFLPAELSLQHPETQIHAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001142685 |
| ORF Size | 6261 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001142685.2 |
| RefSeq Size | 10111 bp |
| RefSeq ORF | 6264 bp |
| Locus ID | 9743 |
| UniProt ID | A7KAX9 |
| Protein Families | Druggable Genome |
| MW | 231 kDa |
| Gene Summary | RICS is a neuron-associated GTPase-activating protein that may regulate dendritic spine morphology and strength by modulating Rho GTPase (see RHOA; MIM 165390) activity (Okabe et al., 2003 [PubMed 12531901]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227921L1 | Lenti ORF clone of Human Rho GTPase activating protein 32 (ARHGAP32), transcript variant 1, Myc-DDK-tagged |
CNY 23,064.00 |
|
| RG227921 | ARHGAP32 (tGFP-tagged) - Human Rho GTPase activating protein 32 (ARHGAP32), transcript variant 1 |
CNY 20,900.00 |
|
| SC326426 | ARHGAP32 (untagged)-Human Rho GTPase activating protein 32 (ARHGAP32), transcript variant 1 |
CNY 40,190.00 |
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