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ROBO2 (NM_001128929) Human Tagged ORF Clone
CAT＃: RC226390
ROBO2 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 1
ORF Plasmid: tGFP
"NM_001128929" in other vectors (6)
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CNY 9,536.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SAX3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226390 representing NM_001128929
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGAAGACATGAACGTGTCACTAGAAGGATGTGGACATGGGCTCCGGGACTGTTGATGATGACTG TGGTGTTTTGGGGTCATCAGGGGAATGGACAAGGCCAAGGATCGCGTCTTCGCCAGGAGGACTTTCCCCC GCGGATTGTGGAGCATCCTTCCGATGTCATCGTCTCTAAGGGCGAGCCCACGACTCTGAACTGCAAGGCG GAGGGCCGGCCAACGCCCACCATTGAGTGGTACAAAGATGGGGAGCGAGTGGAGACTGACAAGGACGATC CCCGGTCCCACAGGATGCTTCTGCCCAGCGGATCCTTATTCTTCTTGCGCATCGTGCACGGGCGCAGGAG TAAACCTGATGAAGGAAGCTACGTTTGTGTTGCGAGGAACTATCTTGGTGAAGCAGTGAGTCGAAATGCG TCTCTGGAAGTGGCATTGTTACGAGATGACTTCCGACAAAACCCCACAGATGTTGTAGTGGCAGCTGGAG AGCCTGCAATCCTGGAGTGCCAGCCTCCCCGGGGACACCCAGAACCCACCATCTACTGGAAAAAAGACAA AGTTCGAATTGATGACAAGGAAGAAAGAATAAGTATCCGTGGTGGAAAACTGATGATCTCCAATACCAGG AAAAGTGATGCAGGGATGTATACTTGTGTTGGTACCAATATGGTGGGAGAAAGGGACAGTGACCCAGCAG AGCTGACTGTCTTTGAACGACCCACATTTCTCAGGAGGCCAATTAACCAGGTGGTACTGGAGGAAGAAGC TGTAGAATTTCGTTGTCAAGTCCAAGGAGATCCTCAACCAACTGTGAGGTGGAAAAAGGATGATGCAGAC TTGCCAAGAGGAAGGTATGACATCAAAGACGATTACACACTAAGAATTAAAAAGACCATGAGTACAGATG AAGGCACCTATATGTGTATTGCTGAGAATCGGGTTGGAAAAATGGAAGCCTCTGCTACACTCACCGTCCG AGCTCCCCCACAGTTTGTGGTTCGGCCAAGAGATCAGATTGTTGCTCAAGGTCGAACAGTGACATTTCCC TGTGAAACTAAAGGAAACCCACAGCCAGCTGTTTTTTGGCAGAAAGAAGGCAGCCAGAACCTACTTTTCC CAAACCAACCCCAGCAGCCCAACAGTAGATGCTCAGTGTCACCAACTGGAGACCTCACAATCACCAACAT TCAACGTTCCGACGCGGGTTACTACATCTGCCAGGCTTTAACTGTGGCAGGAAGCATTTTAGCAAAAGCT CAACTGGAGGTTACTGATGTTTTGACAGATAGACCTCCACCTATAATTCTACAAGGCCCAGCCAACCAAA CGCTGGCAGTGGATGGTACAGCGTTACTGAAATGTAAAGCCACTGGTGATCCTCTTCCTGTAATTAGCTG GTTAAAGGAGGGATTTACTTTTCCGGGTAGAGATCCAAGAGCAACAATTCAAGAGCAAGGCACACTGCAG ATTAAGAATTTACGGATTTCTGATACTGGCACTTATACTTGTGTGGCTACAAGTTCAAGTGGAGAGACTT CCTGGAGTGCAGTGCTGGATGTGACAGAGTCTGGAGCAACAATCAGTAAAAACTATGATTTAAGTGACCT GCCAGGGCCACCATCCAAACCGCAGGTCACTGATGTTACTAAGAACAGTGTCACCTTGTCCTGGCAGCCA GGTACCCCTGGAACCCTTCCAGCAAGTGCATATATCATTGAGGCTTTCAGCCAATCAGTGAGCAACAGCT GGCAGACCGTGGCAAACCATGTAAAGACCACCCTCTATACTGTAAGAGGACTGCGGCCCAATACAATCTA CTTATTCATGGTCAGAGCGATCAACCCCCAAGGTCTCAGTGACCCAAGTCCCATGTCAGATCCTGTGCGC ACACAAGATATCAGCCCACCAGCACAAGGAGTGGACCACAGGCAAGTGCAGAAAGAGCTAGGAGATGTCC TTGTCCGTCTTCATAATCCAGTTGTGCTGACTCCCACCACGGTTCAGGTCACATGGACGGTTGATCGCCA ACCCCAGTTTATCCAAGGCTACCGAGTGATGTATCGTCAGACTTCAGGTCTGCAGGCGACATCTTCGTGG CAGAATTTAGATGCCAAAGTCCCGACTGAACGAAGTGCTGTCTTAGTCAACCTGAAAAAGGGGGTGACTT ATGAAATTAAAGTACGGCCATATTTTAATGAGTTCCAAGGAATGGATAGTGAATCTAAAACGGTTCGTAC TACTGAAGAAGCCCCAAGTGCCCCACCACAGTCTGTCACTGTACTGACAGTTGGAAGCTACAATAGCACA AGTATTAGTGTTTCCTGGGATCCTCCTCCTCCAGATCACCAGAATGGAATTATCCAAGAATACAAGATCT GGTGTCTAGGAAATGAAACGCGATTCCATATCAACAAAACTGTGGATGCAGCCATTCGGTCCGTAATAAT TGGTGGATTATTCCCAGGTATTCAATACCGGGTAGAGGTTGCAGCTAGTACCAGTGCAGGGGTTGGAGTA AAGAGTGAGCCACAGCCAATAATAATCGGGAGACGCAATGAAGTTGTCATTACTGAAAACAATAACAGCA TAACTGAGCAAATCACTGATGTGGTGAAGCAACCAGCCTTTATAGCTGGTATTGGTGGTGCCTGCTGGGT AATTCTGATGGGTTTTAGCATATGGTTGTATTGGCGAAGAAAGAAGAGGAAGGGACTCAGTAATTATGCT GTTACGTTTCAAAGAGGAGATGGAGGACTAATGAGCAATGGAAGCCGTCCAGGTCTTCTCAATGCTGGTG ATCCCAGCTATCCATGGCTTGCTGATTCTTGGCCAGCCACGAGCTTGCCAGTAAATAATAGCAACAGTGG CCCAAATGAGATTGGAAATTTTGGCCGTGGAGATGTGCTGCCACCAGTTCCAGGCCAAGGGGATAAAACA GCAACGATGCTCTCAGATGGAGCCATTTATAGTAGCATTGACTTCACTACCAAAACCAGTTACAACAGTT CCAGCCAAATAACACAGGCTACCCCATATGCCACGACACAGATCTTGCATTCCAACAGCATACATGAATT GGCTGTCGATCTGCCTGATCCACAATGGAAAAGCTCAATTCAGCAAAAAACAGATCTGATGGGATTTGGT TATTCTCTACCTGATCAGAACAAAGGTAACAATGGTGGGAAAGGTGGAAAAAAGAAGAAAAATAAAAACT CTTCTAAACCACAGAAAAACAATGGATCCACTTGGGCCAATGTCCCTCTACCTCCCCCCCCAGTCCAGCC CCTTCCTGGCACGGAGCTGGAACACTATGCAGTGGAACAACAAGAAAATGGCTATGACAGTGATAGCTGG TGCCCACCATTGCCAGTACAAACTTACTTACACCAAGGTCTGGAAGATGAACTGGAAGAAGATGATGATA GGGTCCCAACACCTCCTGTTCGAGGCGTGGCTTCTTCTCCTGCTATCTCCTTTGGACAGCAGTCCACTGC AACTCTTACTCCATCCCCACGGGAAGAGATGCAACCCATGCTGCAGGCTCACCTGGATGAGTTGACAAGA GCCTATCAGTTTGATATAGCAAAACAAACATGGCACATTCAAAGCAATAATCAACCTCCACAGCCTCCAG TTCCACCGTTAGGTTATGTGTCTGGAGCCTTGATTTCTGATTTGGAAACGGATGTTGCAGATGATGATGC CGACGACGAAGAGGAAGCTTTAGAAATCCCCAGGCCCCTGAGAGCACTGGACCAGACTCCTGGATCCAGC ATGGACAATCTAGACAGCTCTGTGACAGGAAAAGCCTTTACCTCCTCTCAAAGACCTCGACCTACCAGCC CATTTTCTACTGACAGTAACACCAGTGCAGCCCTGAGTCAAAGTCAGAGGCCTCGGCCCACTAAAAAACA CAAGGGAGGGCGGATGGACCAACAACCAGCATTGCCTCATCGAAGGGAAGGAATGACAGATGAGGAGGCC TTGGTGCCCTATAGCAAGCCCAGTTTCCCATCTCCAGGTGGCCACAGCTCATCAGGAACAGCTTCTTCTA AGGGATCCACTGGACCTAGGAAAACCGAGGTGTTGAGAGCAGGCCACCAGCGCAATGCCAGCGACCTTCT TGACATAGGATATATGGGCTCCAACAGTCAAGGACAGTTTACAGGTGAATTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226390 representing NM_001128929
Red=Cloning site Green=Tags(s) MARRHERVTRRMWTWAPGLLMMTVVFWGHQGNGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKA EGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNA SLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTR KSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDAD LPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFP CETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKA QLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQ IKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQP GTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVR TQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSW QNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNST SISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGV KSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYA VTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKT ATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFG YSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSW CPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTR AYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSS MDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEA LVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001128929 |
| ORF Size | 4182 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001128929.3, NP_001122401.1 |
| RefSeq ORF | 4185 bp |
| Locus ID | 6092 |
| UniProt ID | Q9HCK4 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Axon guidance |
| MW | 152.9 kDa |
| Gene Summary | The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226390L1 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 1, Myc-DDK-tagged |
CNY 11,936.00 |
|
| RC226390L2 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 1, mGFP tagged |
CNY 15,770.00 |
|
| RC226390L3 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 1, Myc-DDK-tagged |
CNY 11,936.00 |
|
| RC226390L4 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 1, mGFP tagged |
CNY 15,770.00 |
|
| RG226390 | ROBO2 (tGFP-tagged) - Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 1 |
CNY 15,300.00 |
|
| SC323277 | ROBO2 (untagged)-Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 1 |
CNY 26,890.00 |
