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ARHGEF7 (NM_001113512) Human Tagged ORF Clone
CAT＃: RC226137
- TrueORF®
ARHGEF7 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 4
ORF Plasmid: tGFP
"NM_001113512" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BETA-PIX; COOL-1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226137 representing NM_001113512
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCCGCCGAGCAAACCGTTACGTGGCTCATCACTCTGGGGGTGCTGGAGTCGCCCAAAAAAACCA TCTCGGACCCGGAGGGCTTTCTGCAGGCGTCGCTGAAGGATGGGGTGGTCCTCTGCAGGCTGCTGGAGCG CCTGCTCCCCGGGACCATCGAGAAAACGTTTGATGCAAATGATTTGTATCAGGGGCAGAATTTTAACAAG GTCCTCAGTTCCTTAGTGACTCTAAATAAAGTAACAGCAGACATCGGGCTGGGGAGTGACTCCGTGTGTG CCCGGCCCTCGTCTCACCGCATAAAGTCTTTTGACTCCCTTGGATCACAGTCTTTGCACACTCGGACTTC AAAACTGTTCCAGGGCCAGTATCGGAGTTTGGACATGACCGATAATAGCAACAATCAACTGGTAGTAAGA GCAAAGTTTAACTTCCAGCAGACCAATGAGGACGAGCTTTCCTTCTCAAAAGGAGACGTCATCCATGTCA CCCGTGTGGAAGAGGGAGGCTGGTGGGAGGGCACACTCAACGGCCGGACCGGCTGGTTCCCCAGCAACTA CGTGCGCGAGGTCAAGGCCAGCGAGAAGCCTGTGTCTCCCAAATCAGGAACACTGAAGAGCCCTCCCAAA GGATTTGATACGACTGCCATAAACAAAAGCTATTACAATGTGGTGCTACAGAATATTTTAGAAACAGAAA ATGAATATTCTAAAGAACTTCAGACTGTGCTTTCAACGTACCTACGGCCATTGCAGACCAGTGAGAAGTT AAGTTCAGCAAACATTTCATATTTAATGGGAAATCTAGAAGAAATATGTTCTTTCCAGCAAATGCTCGTA CAGTCTTTAGAAGAATGCACCAAGTTGCCCGAAGCTAAGCAGAGAGTCGGAGGCTGCTTTTTAAACCTGA TGCCACAGATGAAAACCCTGTACCTCACGTATTGTGCCAATCACCCTTCTGCAGTGAATGTCCTCACGGA ACACAGTGAGGAGTTGGGGGAGTTCATGGAGACCAAAGGTGCCAGCAGCCCTGGGATTCTCGTGCTGACC ACGGGCCTGAGCAAACCCTTCATGCGCCTGGATAAATACCCTACGCTGCTCAAAGAGCTCGAGAGACACA TGGAGGATTATCATACAGATAGACAAGATATTCAAAAATCCATGGCTGCCTTCAAAAACCTTTCAGCCCA ATGTCAAGAAGTCCGGAAGAGGAAAGAGCTTGAGCTGCAGATCCTGACGGAAGCCATCCGGAACTGGGAG GGCGATGACATTAAAACTCTGGGCAACGTCACTTACATGTCCCAGGTCCTGATTCAGTGTGCCGGAAGTG AGGAAAAGAATGAAAGATATCTTCTACTCTTCCCAAATGTTTTGCTAATGTTGTCTGCCAGTCCTAGGAT GAGTGGCTTTATCTATCAGGGAAAGCTTCCAACGACAGGAATGACAATCACAAAGCTTGAGGACAGTGAA AATCATAGAAATGCATTTGAAATATCAGGGAGCGTGATTGAGCGGATATTAGTGTCGTGCAACAACCAGC AGGATCTGCAGGAATGGGTGGAGCACCTACAGAAGCAAACGAAGGTCACGTCTGTGGGAAACCCCACCAT AAAGCCTCATTCAGTGCCATCTCATACCCTCCCCTCCCACCCGGTCACTCCGTCCAGCAAGCACGCAGAC AGCAAGCCCGCGCCGCTGACGCCCGCCTACCACACGCTGCCCCACCCCTCCCACCACGGCACCCCGCACA CCACCATCAACTGGGGACCCCTGGAGCCTCCGAAAACACCCAAGCCCTGGAGCCTGAGCTGCCTGCGGCC CGCGCCTCCCCTCCGGCCCTCAGCTGCTCTCTGCTACAAGGAGGATCTTAGTAAGAGCCCTAAGACCATG AAAAAGCTGCTGCCCAAGCGCAAACCTGAACGGAAGCCTTCAGATGAGGAGTTCGCGTCCCGGAAAAGCA CAGCTGCTTTGGAAGAAGATGCTCAGATTCTGAAAGTCATTGAAGCTTACTGCACCAGCGCCAAAACAAG GCAAACACTCAATTCAACATGGCAAGGCACTGACCTGTTGCATAATCACGTCTTGGCTGATGATGACCAA CCAAGCCTAGACTCCCTGGGGCGTCGCAGTAGCCTTTCTCGTTTGGAGCCTTCAGACCTCTCGGAAGACT CTGACTATGACAGTATATGGACAGCCCATAGTTACAGAATGGGTTCTACATCTCGTAAGAGCTGTTGCTC ATATATCTCTCACCAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226137 representing NM_001113512
Red=Cloning site Green=Tags(s) MNSAEQTVTWLITLGVLESPKKTISDPEGFLQASLKDGVVLCRLLERLLPGTIEKTFDANDLYQGQNFNK VLSSLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQSLHTRTSKLFQGQYRSLDMTDNSNNQLVVR AKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTGWFPSNYVREVKASEKPVSPKSGTLKSPPK GFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTYLRPLQTSEKLSSANISYLMGNLEEICSFQQMLV QSLEECTKLPEAKQRVGGCFLNLMPQMKTLYLTYCANHPSAVNVLTEHSEELGEFMETKGASSPGILVLT TGLSKPFMRLDKYPTLLKELERHMEDYHTDRQDIQKSMAAFKNLSAQCQEVRKRKELELQILTEAIRNWE GDDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPTTGMTITKLEDSE NHRNAFEISGSVIERILVSCNNQQDLQEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLPSHPVTPSSKHAD SKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPKTPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTM KKLLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLLHNHVLADDDQ PSLDSLGRRSSLSRLEPSDLSEDSDYDSIWTAHSYRMGSTSRKSCCSYISHQN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001113512 |
| ORF Size | 2259 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001113512.2 |
| RefSeq ORF | 2262 bp |
| Locus ID | 8874 |
| UniProt ID | Q14155 |
| Protein Pathways | Regulation of actin cytoskeleton |
| MW | 84.3 kDa |
| Gene Summary | This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226137L3 | Lenti-ORF clone of ARHGEF7 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 4 |
CNY 5,890.00 |
|
| RC226137L4 | Lenti-ORF clone of ARHGEF7 (mGFP-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 4 |
CNY 5,890.00 |
|
| RG226137 | ARHGEF7 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 4 |
CNY 4,370.00 |
|
| SC318975 | ARHGEF7 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 4 |
CNY 7,220.00 |
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