USP46 (NM_001134223) Human Tagged ORF Clone
CAT#: RC225534
- TrueORF®
USP46 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 46 (USP46), transcript variant 2
ORF Plasmid: tGFP
"NM_001134223" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225534 representing NM_001134223
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTGTTTTCAGGGCACCAATGCCTCTGCTCTGGAAAAAGACATTGGTCCAGAGCAGTTTCCAATCA ATGAACACTATTTCGGATTGGTCAATTTTGGAAACACATGCTACTGTAACTCCGTGCTTCAGGCATTGTA CTTCTGCCGTCCATTCCGGGAGAATGTGTTGGCATACAAGGCCCAGCAAAAGAAGAAGGAAAACTTGCTG ACGTGCCTGGCGGACCTTTTCCACAGCATTGCCACACAGAAGAAGAAGGTTGGCGTCATCCCACCAAAGA AGTTCATTTCAAGGCTGAGAAAAGAGAATGATCTCTTTGATAACTACATGCAGCAGGATGCTCATGAATT TTTAAATTATTTGCTAAACACTATTGCGGACATCCTTCAGGAGGAGAAGAAACAGGAAAAACAAAATGGA AAATTAAAAAATGGCAACATGAACGAACCTGCGGAAAATAATAAACCAGAACTCACCTGGGTCCATGAGA TTTTTCAGGGAACGCTTACCAATGAAACTCGATGCTTGAACTGTGAAACTGTTAGTAGCAAAGATGAAGA TTTTCTTGACCTTTCTGTTGATGTGGAGCAGAATACATCCATTACCCACTGTCTAAGAGACTTCAGCAAC ACAGAAACACTGTGTAGTGAACAAAAATATTATTGTGAAACATGCTGCAGCAAACAAGAAGCCCAGAAAA GGATGAGGGTAAAAAAGCTGCCCATGATCTTGGCCCTGCACCTAAAGCGGTTCAAGTACATGGAGCAGCT GCACAGATACACCAAGCTGTCTTACCGTGTGGTCTTCCCTCTGGAACTCCGGCTCTTCAACACCTCCAGT GATGCAGTGAACCTGGACCGCATGTATGACTTGGTTGCGGTGGTCGTTCACTGTGGCAGTGGTCCTAATC GTGGGCATTATATCACTATTGTGAAAAGTCACGGCTTCTGGCTTTTGTTTGATGATGACATTGTAGAGAA AATAGATGCTCAAGCTATTGAAGAATTCTATGGCCTGACGTCAGATATATCAAAAAATTCAGAATCTGGA TATATTTTATTCTATCAGTCAAGAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225534 representing NM_001134223
Red=Cloning site Green=Tags(s) MNCFQGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQKKKENLL TCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNG KLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSN TETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSS DAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESG YILFYQSRE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001134223 |
ORF Size | 1077 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001134223.2 |
RefSeq ORF | 1080 bp |
Locus ID | 64854 |
UniProt ID | P62068 |
Protein Families | Druggable Genome, Protease |
MW | 41.6 kDa |
Gene Summary | Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM, Jun 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225534L3 | Lenti-ORF clone of USP46 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 46 (USP46), transcript variant 2 |
CNY 5,890.00 |
|
RC225534L4 | Lenti-ORF clone of USP46 (mGFP-tagged)-Human ubiquitin specific peptidase 46 (USP46), transcript variant 2 |
CNY 5,890.00 |
|
RG225534 | USP46 (tGFP-tagged) - Human ubiquitin specific peptidase 46 (USP46), transcript variant 2 |
CNY 4,370.00 |
|
SC325818 | USP46 (untagged)-Human ubiquitin specific peptidase 46 (USP46), transcript variant 2 |
CNY 5,800.00 |