CRLS1 (NM_001127458) Human Tagged ORF Clone
CAT#: RC225229
CRLS1 (Myc-DDK-tagged)-Human cardiolipin synthase 1 (CRLS1), transcript variant 2
ORF Plasmid: tGFP
"NM_001127458" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C20orf155; CLS; CLS1; dJ967N21.6; GCD10 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225229 representing NM_001127458
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACAGTATGAAAACCCATGGACAATCCCGAATATGTTGTCAATGACGAGAATTGGCTTGGCCCCAG TTCTGGGCTATTTGATTATTGAAGAAGATTTTAATATTGCACTAGGAGTTTTTGCTTTAGCTGGACTAAC AGATTTGTTGGATGGATTTATTGCTCGAAACTGGGCCAATCAAAGATCAGCTTTGGGAAGTGCTCTTGAT CCACTTGCTGATAAAATACTTATCAGTATCTTATATGTTAGCTTGACCTATGCAGATCTTATTCCAGTTC CACTTACTTACATGATCATTTCGAGAGATGTAATGTTGATTGCTGCTGTTTTTTATGTCAGATACCGAAC TCTTCCAACACCACGAACACTTGCCAAGTATTTCAATCCTTGCTATGCCACTGCTAGGTTAAAACCAACA TTCATCAGCAAGGTGAATACAGCAGTCCAGTTAATCTTGGTGGCAGCTTCTTTGGCAGCTCCAGTTTTCA ACTATGCTGACAGCATTTATCTTCAGATACTATGGTGTTTTACAGCTTTCACCACAGCTGCATCAGCTTA TAGTTACTATCATTATGGCCGGAAGACTGTTCAGGTGATAAAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225229 representing NM_001127458
Red=Cloning site Green=Tags(s) MPQYENPWTIPNMLSMTRIGLAPVLGYLIIEEDFNIALGVFALAGLTDLLDGFIARNWANQRSALGSALD PLADKILISILYVSLTYADLIPVPLTYMIISRDVMLIAAVFYVRYRTLPTPRTLAKYFNPCYATARLKPT FISKVNTAVQLILVAASLAAPVFNYADSIYLQILWCFTAFTTAASAYSYYHYGRKTVQVIKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001127458 |
ORF Size | 606 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001127458.2 |
RefSeq ORF | 609 bp |
Locus ID | 54675 |
UniProt ID | Q9UJA2 |
Protein Families | Transmembrane |
Protein Pathways | Glycerophospholipid metabolism, Metabolic pathways |
MW | 22.4 kDa |
Gene Summary | This gene encodes a member of the CDP-alcohol phosphatidyltransferase class-I family of proteins. The encoded enzyme catalyzes the synthesis of cardiolipin, a phospholipid component of mitochondrial membranes that is critical for mitochondrial function. [provided by RefSeq, Apr 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225229L3 | Lenti ORF clone of Human cardiolipin synthase 1 (CRLS1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC225229L4 | Lenti ORF clone of Human cardiolipin synthase 1 (CRLS1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG225229 | CRLS1 (tGFP-tagged) - Human cardiolipin synthase 1 (CRLS1), transcript variant 2 |
CNY 4,370.00 |
|
SC322890 | CRLS1 (untagged)-Human cardiolipin synthase 1 (CRLS1), transcript variant 2 |
CNY 3,990.00 |