Plexin B1 (PLXNB1) (NM_002673) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PLEXIN-B1; PLXN5; SEP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC224819 representing NM_002673 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCTCTGGGCCCAGCTCTTCTCCAGGCTCTCTGGGCCGGGTGGGTCCTCACCCTCCAGCCCCTTC CACCAACTGCATTCACTCCCAATGGCACGTATCTGCAGCACCTGGCAAGGGACCCCACCTCAGGCACCCT CTACCTGGGGGCTACCAACTTCCTGTTCCAGCTGAGCCCTGGGCTGCAGCTGGAGGCCACAGTGTCCACC GGCCCTGTGCTAGACAGCAGGGACTGCCTGCCACCTGTGATGCCTGATGAGTGCCCCCAGGCCCAGCCTA CCAACAACCCGAATCAGCTGCTCCTGGTGAGCCCAGGGGCCCTGGTGGTATGCGGGAGCGTGCACCAGGG GGTCTGTGAACAGCGGCGCCTGGGGCAGCTCGAGCAGCTGCTGCTGCGGCCAGAGCGGCCTGGGGACACA CAATATGTGGCTGCCAATGATCCTGCGGTCAGCACGGTGGGGCTGGTAGCCCAGGGCTTGGCAGGGGAGC CCCTCCTGTTTGTGGGGCGAGGATACACCAGCAGGGGTGTGGGGGGTGGCATTCCACCCATCACAACCCG GGCCCTGTGGCCGCCCGACCCCCAAGCTGCCTTCTCCTATGAGGAGACAGCCAAGCTGGCAGTGGGCCGC CTCTCCGAGTACAGCCACCACTTCGTGAGTGCCTTTGCACGTGGGGCCAGCGCCTACTTCCTGTTCCTGC GGCGGGACCTGCAGGCTCAGTCTAGAGCTTTTCGTGCCTATGTATCTCGAGTGTGTCTCCGGGACCAGCA CTACTACTCCTATGTGGAGTTGCCTCTGGCCTGCGAAGGTGGCCGCTACGGGCTGATCCAGGCTGCAGCT GTGGCCACGTCCAGGGAGGTGGCGCATGGGGAGGTGCTCTTTGCAGCTTTCTCCTCGGCTGCACCCCCCA CTGTGGGCCGGCCCCCATCGGCGGCTGCTGGGGCATCTGGAGCCTCTGCCCTCTGTGCCTTCCCCCTGGA TGAGGTGGACCGGCTTGCTAATCGCACGCGAGATGCCTGCTACACCCGGGAGGGTCGTGCTGAGGATGGG ACCGAGGTGGCCTACATCGAGTATGATGTCAATTCTGACTGTGCACAGCTGCCAGTGGACACCCTGGATG CTTATCCCTGTGGCTCAGACCACACGCCCAGCCCCATGGCCAGCCGGGTCCCGCTGGAAGCCACACCAAT TCTGGAGTGGCCAGGGATTCAGCTAACAGCTGTGGCAGTCACCATGGAAGATGGACACACCATCGCTTTC CTGGGTGATAGTCAAGGGCAGCTGCACAGGGTCTACTTGGGCCCAGGGAGCGATGGCCACCCATACTCCA CACAGAGCATCCAGCAGGGGTCTGCAGTGAGCAGAGACCTCACCTTTGATGGGACCTTTGAGCACCTGTA TGTCATGACCCAGAGCACACTTCTGAAGGTTCCTGTGGCTTCCTGTGCTCAGCACCTGGACTGTGCATCT TGCCTTGCTCACAGGGACCCATACTGTGGGTGGTGCGTGCTCCTTGGCAGGTGCAGTCGCCGTTCTGAGT GCTCGAGGGGCCAGGGCCCAGAGCAGTGGCTATGGAGCTTCCAGCCTGAGCTGGGCTGTCTGCAAGTGGC AGCCATGAGTCCTGCCAACATCAGCCGAGAGGAGACGAGGGAGGTTTTCCTATCAGTGCCAGACCTGCCA CCCCTGTGGCCAGGGGAGTCATATTCCTGCCACTTTGGGGAACATCAGAGTCCTGCCCTGCTGACTGGTT CTGGTGTGATGTGCCCCTCCCCAGACCCTAGTGAGGCCCCAGTGCTGCCGAGAGGAGCCGACTACGTATC CGTGAGCGTGGAGCTCAGATTTGGCGCTGTTGTGATCGCCAAAACTTCCCTCTCTTTCTATGACTGTGTG GCGGTCACTGAACTCCGCCCATCTGCGCAGTGCCAGGCCTGTGTGAGCAGCCGCTGGGGGTGTAACTGGT GTGTCTGGCAGCACCTGTGCACCCACAAGGCCTCGTGTGATGCTGGGCCCATGGTTGCAAGCCATCAGAG CCCGCTTGTCTCCCCAGACCCTCCTGCAAGAGGTGGACCCAGCCCCTCCCCACCCACAGCCCCCAAAGCC CTGGCCACCCCTGCTCCTGACACCCTTCCCGTGGAGCCTGGGGCTCCCTCCACAGCCACAGCTTCGGACA TCTCACCTGGGGCTAGTCCTTCCCTGCTCAGCCCCTGGGGGCCATGGGCAGGTTCTGGCTCCATATCTTC CCCTGGCTCCACAGGGTCGCCTCTCCATGAGGAGCCCTCCCCTCCCAGCCCCCAAAATGGACCTGGAACC GCTGTCCCTGCCCCCACTGACTTCAGACCCTCAGCCACACCTGAGGACCTCTTGGCCTCCCCGCTGTCAC CGTCAGAGGTAGCAGCAGTGCCCCCTGCAGACCCTGGCCCCGAGGCTCTTCATCCCACAGTGCCCCTGGA CCTGCCCCCTGCCACTGTTCCTGCCACCACTTTCCCAGGGGCCATGGGCTCCGTGAAGCCCGCCCTGGAC TGGCTCACGAGAGAAGGCGGCGAGCTGCCCGAGGCGGACGAGTGGACGGGGGGTGACGCACCCGCCTTCT CCACTTCCACCCTCCTCTCAGGTGATGGAGACTCAGCAGAGCTTGAGGGCCCTCCCGCCCCCCTCATCCT CCCGTCCAGCCTCGACTACCAGTATGACACCCCCGGGCTCTGGGAGCTGGAAGAGGCGACCTTGGGGGCA AGCTCCTGCCCCTGTGTGGAGAGCGTTCAGGGCTCCACGTTGATGCCGGTCCATGTGGAGCGGGAAATCC GGCTGCTAGGCAGGAACCTGCACCTTTTCCAGGATGGCCCAGGAGACAATGAGTGTGTGATGGAGCTGGA GGGCCTCGAGGTGGTGGTTGAGGCCCGGGTCGAGTGTGAGCCACCTCCAGATACCCAGTGCCATGTCACC TGCCAGCAGCACCAGCTCAGCTATGAGGCTCTGCAGCCGGAGCTCCGTGTGGGGCTGTTTCTGCGTCGGG CCGGCCGTCTGCGTGTGGACAGTGCTGAGGGGCTGCATGTGGTACTGTATGACTGTTCCGTGGGACATGG AGACTGCAGCCGCTGCCAAACTGCCATGCCCCAGTATGGCTGTGTGTGGTGTGAGGGGGAGCGTCCACGT TGTGTGACCCGGGAGGCCTGTGGTGAGGCTGAGGCTGTGGCCACCCAGTGCCCAGCGCCCCTCATCCACT CGGTGGAGCCACTGACTGGGCCTGTAGACGGAGGCACCCGTGTCACCATCAGGGGCTCCAACCTGGGCCA GCATGTGCAGGATGTGCTGGGCATGGTCACGGTGGCTGGAGTGCCCTGTGCTGTGGATGCCCAGGAGTAC GAGGTCTCCAGCAGCCTCGTGTGCATCACCGGGGCCAGTGGGGAGGAGGTGGCCGGCGCCACAGCGGTGG AGGTGCCGGGAAGAGGACGTGGTGTCTCAGAACACGACTTTGCCTACCAGGATCCGAAGGTCCATTCCAT CTTCCCGGCCCGCGGCCCCAGAGCTGGGGGCACCCGTCTCACCCTGAATGGCTCCAAGCTCCTGACTGGG CGGCTGGAGGACATCCGAGTGGTGGTTGGAGACCAGCCTTGTCACTTGCTGCCGGAGCAGCAGTCAGAAC AACTGCGGTGTGAGACCAGCCCACGCCCCACGCCTGCCACGCTCCCTGTGGCTGTGTGGTTTGGGGCCAC GGAGCGGAGGCTTCAACGCGGACAGTTCAAGTATACCTTGGACCCCAACATCACCTCTGCTGGCCCCACC AAGAGCTTCCTCAGTGGAGGACGTGAGATATGCGTCCGTGGCCAGAATCTGGACGTGGTACAGACGCCAA GAATCCGGGTGACCGTGGTCTCGAGAATGCTGCAGCCCAGCCAGGGGCTTGGACGGAGGCGTCGCGTGGT CCCGGAGACGGCATGTTCCCTTGGACCCTCCTGCAGTAGCCAGCAATTTGAGGAGCCGTGCCATGTCAAC TCCTCCCAGCTCATCACGTGCCGCACACCTGCCCTCCCAGGCCTGCCTGAGGACCCCTGGGTCCGGGTGG AATTTATCCTTGACAACCTGGTCTTTGACTTTGCAACACTGAACCCCACACCTTTCTCCTATGAGGCCGA CCCCACCCTGCAGCCACTCAACCCTGAGGACCCCACCATGCCATTCCGGCACAAGCCTGGGAGTGTGTTC TCCGTGGAGGGGGAGAACCTGGACCTTGCAATGTCCAAGGAGGAGGTGGTGGCTATGATAGGGGATGGCC CCTGTGTGGTGAAGACGCTGACGCGGCACCACCTGTACTGCGAGCCCCCCGTGGAGCAGCCCCTGCCACG GCACCATGCCCTCCGAGAGGCACCTGACTCTTTGCCTGAGTTCACGGTGCAGATGGGGAACTTGCGCTTC TCCCTGGGTCACGTGCAGTATGACGGCGAGAGCCCTGGGGCTTTTCCTGTGGCAGCCCAGGTGGGCTTGG GGGTGGGCACCTCTCTTCTGGCTCTGGGTGTCATCATCATTGTCCTCATGTACAGGAGGAAGAGCAAGCA GGCCCTGAGGGACTATAAGAAGGTTCAGATCCAGCTGGAGAATCTGGAGAGCAGTGTGCGGGACCGCTGC AAGAAGGAATTCACAGACCTCATGACTGAGATGACCGATCTCACCAGTGACCTCCTGGGCAGCGGCATCC CCTTCCTCGACTACAAGGTGTATGCGGAGAGGATCTTCTTCCCTGGGCACCGCGAGTCGCCCTTGCACCG GGACCTGGGTGTGCCTGAGAGCAGACGGCCCACTGTGGAGCAAGGGCTGGGGCAGCTCTCTAACCTGCTC AACAGCAAGCTCTTCCTCACCAAGTTCATCCACACGCTGGAGAGCCAGCGCACCTTTTCAGCTCGGGACC GTGCCTACGTGGCATCTCTGCTCACCGTGGCACTGCATGGGAAGCTTGAGTATTTCACTGACATCCTCCG CACTCTGCTCAGTGACCTGGTTGCCCAGTATGTGGCCAAGAACCCCAAGCTGATGCTGCGCAGGACAGAG ACTGTGGTGGAGAAGCTGCTCACCAACTGGATGTCCATCTGTCTGTATACCTTCGTGAGGGACTCCGTAG GGGAGCCTCTGTACATGCTCTTTCGAGGGATTAAGCACCAAGTGGATAAGGGGCCAGTGGACAGTGTGAC AGGCAAGGCCAAATACACCTTGAACGACAACCGCCTGCTCAGAGAGGATGTGGAGTACCGTCCCCTGACC TTGAATGCACTATTGGCTGTGGGGCCTGGGGCAGGAGAGGCCCAGGGCGTGCCCGTGAAGGTCCTAGACT GTGACACCATCTCCCAGGCAAAGGAGAAGATGCTGGACCAGCTTTATAAAGGAGTGCCTCTCACCCAGCG GCCAGACCCTCGCACCCTTGATGTTGAGTGGCGGTCTGGGGTGGCCGGGCACCTCATTCTTTCTGACGAG GATGTCACTTCTGAGGTCCAGGGTCTGTGGAGGCGCCTGAACACACTGCAGCATTACAAGGTCCCAGATG GAGCAACTGTGGCCCTCGTCCCCTGCCTCACCAAGCATGTGCTCCGGGAAAACCAGGATTATGTCCCTGG AGAGCGGACCCCAATGCTGGAGGATGTAGATGAGGGGGGCATCCGGCCCTGGCACCTGGTGAAGCCAAGT GATGAGCCGGAGCCGCCCAGGCCTCGGAGGGGCAGCCTTCGGGGCGGGGAGCGTGAGCGCGCCAAGGCCA TCCCTGAGATCTACCTGACCCGCCTGCTGTCCATGAAGGGCACCCTGCAGAAGTTCGTGGATGACCTGTT CCAGGTGATTCTCAGCACCAGCCGCCCCGTGCCGCTCGCTGTGAAGTACTTCTTTGACCTGCTGGATGAG CAGGCCCAGCAGCATGGCATCTCCGACCAGGACACCATCCACATCTGGAAGACCAACAGCTTGCCTCTGA GGTTCTGGATCAATATAATAAAAAACCCGCAGTTTGTGTTCGACGTGCAAACATCTGATAACATGGATGC GGTGCTCCTTGTCATTGCACAGACCTTCATGGACGCCTGCACCCTGGCCGACCACAAGCTGGGCCGGGAC TCCCCGATCAACAAACTTCTGTATGCACGGGACATTCCCCGGTACAAGCGGATGGTGGAAAGGTACTATG CAGACATCAGACAGACTGTCCCAGCCAGCGACCAAGAGATGAACTCTGTCCTGGCTGAACTGTCCTGGAA CTACTCCGGAGACCTCGGGGCGCGAGTGGCCCTGCATGAACTCTACAAGTACATCAACAAGTACTATGAC CAGATCATCACTGCCCTGGAGGAGGATGGCACGGCCCAGAAGATGCAGCTGGGCTATCGGCTCCAGCAGA TTGCAGCTGCTGTGGAAAACAAGGTCACAGATCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC224819 representing NM_002673 Red=Cloning site Green=Tags(s) MPALGPALLQALWAGWVLTLQPLPPTAFTPNGTYLQHLARDPTSGTLYLGATNFLFQLSPGLQLEATVST GPVLDSRDCLPPVMPDECPQAQPTNNPNQLLLVSPGALVVCGSVHQGVCEQRRLGQLEQLLLRPERPGDT QYVAANDPAVSTVGLVAQGLAGEPLLFVGRGYTSRGVGGGIPPITTRALWPPDPQAAFSYEETAKLAVGR LSEYSHHFVSAFARGASAYFLFLRRDLQAQSRAFRAYVSRVCLRDQHYYSYVELPLACEGGRYGLIQAAA VATSREVAHGEVLFAAFSSAAPPTVGRPPSAAAGASGASALCAFPLDEVDRLANRTRDACYTREGRAEDG TEVAYIEYDVNSDCAQLPVDTLDAYPCGSDHTPSPMASRVPLEATPILEWPGIQLTAVAVTMEDGHTIAF LGDSQGQLHRVYLGPGSDGHPYSTQSIQQGSAVSRDLTFDGTFEHLYVMTQSTLLKVPVASCAQHLDCAS CLAHRDPYCGWCVLLGRCSRRSECSRGQGPEQWLWSFQPELGCLQVAAMSPANISREETREVFLSVPDLP PLWPGESYSCHFGEHQSPALLTGSGVMCPSPDPSEAPVLPRGADYVSVSVELRFGAVVIAKTSLSFYDCV AVTELRPSAQCQACVSSRWGCNWCVWQHLCTHKASCDAGPMVASHQSPLVSPDPPARGGPSPSPPTAPKA LATPAPDTLPVEPGAPSTATASDISPGASPSLLSPWGPWAGSGSISSPGSTGSPLHEEPSPPSPQNGPGT AVPAPTDFRPSATPEDLLASPLSPSEVAAVPPADPGPEALHPTVPLDLPPATVPATTFPGAMGSVKPALD WLTREGGELPEADEWTGGDAPAFSTSTLLSGDGDSAELEGPPAPLILPSSLDYQYDTPGLWELEEATLGA SSCPCVESVQGSTLMPVHVEREIRLLGRNLHLFQDGPGDNECVMELEGLEVVVEARVECEPPPDTQCHVT CQQHQLSYEALQPELRVGLFLRRAGRLRVDSAEGLHVVLYDCSVGHGDCSRCQTAMPQYGCVWCEGERPR CVTREACGEAEAVATQCPAPLIHSVEPLTGPVDGGTRVTIRGSNLGQHVQDVLGMVTVAGVPCAVDAQEY EVSSSLVCITGASGEEVAGATAVEVPGRGRGVSEHDFAYQDPKVHSIFPARGPRAGGTRLTLNGSKLLTG RLEDIRVVVGDQPCHLLPEQQSEQLRCETSPRPTPATLPVAVWFGATERRLQRGQFKYTLDPNITSAGPT KSFLSGGREICVRGQNLDVVQTPRIRVTVVSRMLQPSQGLGRRRRVVPETACSLGPSCSSQQFEEPCHVN SSQLITCRTPALPGLPEDPWVRVEFILDNLVFDFATLNPTPFSYEADPTLQPLNPEDPTMPFRHKPGSVF SVEGENLDLAMSKEEVVAMIGDGPCVVKTLTRHHLYCEPPVEQPLPRHHALREAPDSLPEFTVQMGNLRF SLGHVQYDGESPGAFPVAAQVGLGVGTSLLALGVIIIVLMYRRKSKQALRDYKKVQIQLENLESSVRDRC KKEFTDLMTEMTDLTSDLLGSGIPFLDYKVYAERIFFPGHRESPLHRDLGVPESRRPTVEQGLGQLSNLL NSKLFLTKFIHTLESQRTFSARDRAYVASLLTVALHGKLEYFTDILRTLLSDLVAQYVAKNPKLMLRRTE TVVEKLLTNWMSICLYTFVRDSVGEPLYMLFRGIKHQVDKGPVDSVTGKAKYTLNDNRLLREDVEYRPLT LNALLAVGPGAGEAQGVPVKVLDCDTISQAKEKMLDQLYKGVPLTQRPDPRTLDVEWRSGVAGHLILSDE DVTSEVQGLWRRLNTLQHYKVPDGATVALVPCLTKHVLRENQDYVPGERTPMLEDVDEGGIRPWHLVKPS DEPEPPRPRRGSLRGGERERAKAIPEIYLTRLLSMKGTLQKFVDDLFQVILSTSRPVPLAVKYFFDLLDE QAQQHGISDQDTIHIWKTNSLPLRFWINIIKNPQFVFDVQTSDNMDAVLLVIAQTFMDACTLADHKLGRD SPINKLLYARDIPRYKRMVERYYADIRQTVPASDQEMNSVLAELSWNYSGDLGARVALHELYKYINKYYD QIITALEEDGTAQKMQLGYRLQQIAAAVENKVTDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_002673
ORF Size	6405 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_002673.4
RefSeq Size	7097 bp
RefSeq ORF	6408 bp
Locus ID	5364
UniProt ID	O43157
Protein Families	Druggable Genome
Protein Pathways	Axon guidance
MW	232.1 kDa
Gene Summary	Receptor for SEMA4D (PubMed:19843518, PubMed:20877282, PubMed:21912513). Plays a role in GABAergic synapse development (By similarity). Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed:12196628, PubMed:15210733). Plays a role in axon guidance, invasive growth and cell migration (PubMed:12198496).[UniProtKB/Swiss-Prot Function]

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