United States
Germany
Japan
United Kingdom
China
KDM4B (NM_015015) Human Tagged ORF Clone
CAT＃: RC223401
- TrueORF®
KDM4B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 4B (KDM4B)
ORF Plasmid: tGFP
"NM_015015" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 11,448.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | JMJD2B; TDRD14B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223401 representing NM_015015
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCTGAGGACCACGGCGCCCAGAACCCCAGCTGTAAAATCATGACGTTTCGCCCAACCATGGAAG AATTTAAAGACTTCAACAAATACGTGGCCTACATAGAGTCGCAGGGAGCCCACCGGGCGGGCCTGGCCAA GATCATCCCCCCGAAGGAGTGGAAGCCGCGGCAGACGTATGATGACATCGACGACGTGGTGATCCCGGCG CCCATCCAGCAGGTGGTGACGGGCCAGTCGGGCCTCTTCACGCAGTACAATATCCAGAAGAAGGCCATGA CAGTGGGCGAGTACCGCCGCCTGGCCAACAGCGAGAAGTACTGTACCCCGCGGCACCAGGACTTTGACGA CCTTGAACGCAAATACTGGAAGAACCTCACCTTTGTCTCCCCGATCTACGGGGCTGACATCAGCGGCTCT TTGTATGATGACGACGTGGCCCAGTGGAACATCGGGAGCCTCCGGACCATCCTGGACATGGTGGAGCGCG AGTGCGGCACCATCATCGAGGGCGTGAACACGCCCTACCTGTACTTCGGCATGTGGAAGACCACCTTCGC CTGGCACACCGAGGACATGGACCTGTACAGCATCAACTACCTGCACTTTGGGGAGCCTAAGTCCTGGTAC GCCATCCCACCAGAGCACGGCAAGCGCCTGGAGCGGCTGGCCATCGGCTTCTTCCCCGGGAGCTCGCAGG GCTGCGACGCCTTCCTGCGGCATAAGATGACCCTCATCTCGCCCATCATCCTGAAGAAGTACGGGATCCC CTTCAGCCGGATCACGCAGGAGGCCGGGGAATTCATGATCACATTTCCCTACGGCTACCACGCCGGCTTC AATCACGGGTTCAACTGCGCAGAATCTACCAACTTCGCCACCCTGCGGTGGATTGACTACGGCAAAGTGG CCACTCAGTGCACGTGCCGGAAGGACATGGTCAAGATCTCCATGGACGTGTTCGTGCGCATCCTGCAGCC CGAGCGCTACGAGCTGTGGAAGCAGGGCAAGGACCTCACGGTGCTGGACCACACGCGGCCCACGGCGCTC ACCAGCCCCGAGCTGAGCTCCTGGAGTGCGTCCCGGGCCTCGCTGAAGGCCAAGCTCCTCCGCAGGTCTC ACCGGAAACGGAGCCAGCCCAAGAAGCCGAAGCCCGAAGACCCCAAGTTCCCTGGGGAGGGTACGGCTGG GGCAGCGCTCCTAGAGGAGGCTGGGGGCAGCGTGAAGGAGGAGGCTGGGCCGGAGGTTGACCCCGAGGAG GAGGAGGAGGAGCCGCAGCCACTGCCACACGGCCGGGAGGCCGAGGGCGCAGAAGAGGACGGGAGGGGCA AGCTGCGGCCAACCAAGGCCAAGAGCGAGCGGAAGAAGAAGAGCTTCGGCCTGCTGCCCCCACAGCTGCC GCCCCCGCCTGCTCACTTCCCCTCAGAGGAGGCGCTGTGGCTGCCATCCCCACTGGAGCCCCCGGTGCTG GGCCCAGGCCCTGCAGCCATGGAGGAGAGCCCCCTGCCGGCACCCCTTAATGTCGTGCCCCCTGAGGTGC CCAGTGAGGAGCTAGAGGCCAAGCCTCGGCCCATCATCCCCATGCTGTACGTGGTGCCGCGGCCGGGCAA GGCAGCCTTCAACCAGGAGCACGTGTCCTGCCAGCAGGCCTTTGAGCACTTTGCCCAGAAGGGTCCGACC TGGAAGGAACCAGTTTCCCCCATGGAGCTGACGGGGCCAGAGGACGGTGCAGCCAGCAGTGGGGCAGGTC GCATGGAGACCAAAGCCCGGGCCGGAGAGGGGCAGGCACCGTCCACATTTTCCAAATTGAAGATGGAGAT CAAGAAGAGCCGGCGCCATCCCCTGGGCCGGCCGCCCACCCGGTCCCCACTGTCGGTGGTGAAGCAGGAG GCCTCAAGTGACGAGGAGGCATCCCCTTTCTCCGGGGAGGAAGATGTGAGTGACCCGGACGCCTTGAGGC CGCTGCTGTCTCTGCAGTGGAAGAACAGGGCGGCCAGCTTCCAGGCCGAGAGGAAGTTCAACGCAGCGGC TGCGCGCACGGAGCCCTACTGCGCCATCTGCACGCTCTTCTACCCCTACTGCCAGGCCCTACAGACTGAG AAGGAGGCACCCATAGCCTCCCTCGGAGAGGGCTGCCCGGCCACATTACCCTCCAAAAGCCGTCAGAAGA CCCGACCGCTCATCCCTGAGATGTGCTTCACCTCTGGCGGTGAGAACACGGAGCCGCTGCCTGCCAACTC CTACATCGGCGACGACGGGACCAGCCCCCTGATCGCCTGCGGCAAGTGCTGCCTGCAGGTCCATGCCAGT TGCTATGGCATCCGTCCCGAGCTGGTCAATGAAGGCTGGACGTGTTCCCGGTGCGCGGCCCACGCCTGGA CTGCGGAGTGCTGCCTGTGCAACCTGCGAGGAGGTGCGCTGCAGATGACCACCGATAGGAGGTGGATCCA CGTGATCTGTGCCATCGCAGTCCCCGAGGCGCGCTTCCTGAACGTGATTGAGCGCCACCCTGTGGACATC AGCGCCATCCCCGAGCAGCGGTGGAAGCTGAAATGCGTGTACTGCCGGAAGCGGATGAAGAAGGTGTCAG GTGCCTGTATCCAGTGCTCCTACGAGCACTGCTCCACGTCCTTCCACGTGACCTGCGCCCACGCCGCAGG CGTGCTCATGGAGCCGGACGACTGGCCCTATGTGGTCTCCATCACCTGCCTCAAGCACAAGTCGGGGGGT CACGCTGTCCAACTCCTGAGGGCCGTGTCCCTAGGCCAGGTGGTCATCACCAAGAACCGCAACGGGCTGT ACTACCGCTGTCGCGTCATCGGTGCCGCCTCGCAGACCTGCTACGAAGTGAACTTCGACGATGGCTCCTA CAGCGACAACCTGTACCCTGAGAGCATCACGAGTAGGGACTGTGTCCAGCTGGGACCCCCTTCCGAGGGG GAGCTGGTGGAGCTCCGGTGGACTGACGGCAACCTCTACAAGGCCAAGTTCATCTCCTCCGTCACCAGCC ACATCTACCAGGTGGAGTTTGAGGACGGGTCCCAGCTGACGGTGAAGCGTGGGGACATCTTCACCCTGGA GGAGGAGCTGCCCAAGAGGGTCCGCTCTCGGCTGTCACTGAGCACGGGGGCACCGCAGGAGCCCGCCTTC TCGGGGGAGGAGGCCAAGGCCGCCAAGCGCCCGCGTGTGGGCACCCCGCTTGCCACGGAGGACTCCGGGC GGAGCCAGGACTACGTGGCCTTCGTGGAGAGCCTCCTGCAGGTGCAGGGCCGGCCCGGAGCCCCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223401 representing NM_015015
Red=Cloning site Green=Tags(s) MGSEDHGAQNPSCKIMTFRPTMEEFKDFNKYVAYIESQGAHRAGLAKIIPPKEWKPRQTYDDIDDVVIPA PIQQVVTGQSGLFTQYNIQKKAMTVGEYRRLANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGS LYDDDVAQWNIGSLRTILDMVERECGTIIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSWY AIPPEHGKRLERLAIGFFPGSSQGCDAFLRHKMTLISPIILKKYGIPFSRITQEAGEFMITFPYGYHAGF NHGFNCAESTNFATLRWIDYGKVATQCTCRKDMVKISMDVFVRILQPERYELWKQGKDLTVLDHTRPTAL TSPELSSWSASRASLKAKLLRRSHRKRSQPKKPKPEDPKFPGEGTAGAALLEEAGGSVKEEAGPEVDPEE EEEEPQPLPHGREAEGAEEDGRGKLRPTKAKSERKKKSFGLLPPQLPPPPAHFPSEEALWLPSPLEPPVL GPGPAAMEESPLPAPLNVVPPEVPSEELEAKPRPIIPMLYVVPRPGKAAFNQEHVSCQQAFEHFAQKGPT WKEPVSPMELTGPEDGAASSGAGRMETKARAGEGQAPSTFSKLKMEIKKSRRHPLGRPPTRSPLSVVKQE ASSDEEASPFSGEEDVSDPDALRPLLSLQWKNRAASFQAERKFNAAAARTEPYCAICTLFYPYCQALQTE KEAPIASLGEGCPATLPSKSRQKTRPLIPEMCFTSGGENTEPLPANSYIGDDGTSPLIACGKCCLQVHAS CYGIRPELVNEGWTCSRCAAHAWTAECCLCNLRGGALQMTTDRRWIHVICAIAVPEARFLNVIERHPVDI SAIPEQRWKLKCVYCRKRMKKVSGACIQCSYEHCSTSFHVTCAHAAGVLMEPDDWPYVVSITCLKHKSGG HAVQLLRAVSLGQVVITKNRNGLYYRCRVIGAASQTCYEVNFDDGSYSDNLYPESITSRDCVQLGPPSEG ELVELRWTDGNLYKAKFISSVTSHIYQVEFEDGSQLTVKRGDIFTLEEELPKRVRSRLSLSTGAPQEPAF SGEEAKAAKRPRVGTPLATEDSGRSQDYVAFVESLLQVQGRPGAPF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015015 |
| ORF Size | 3288 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015015.1, NP_055830.1 |
| RefSeq Size | 5595 bp |
| RefSeq ORF | 3291 bp |
| Locus ID | 23030 |
| UniProt ID | O94953 |
| Protein Families | Druggable Genome |
| MW | 121.7 kDa |
| Gene Summary | Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223401L1 | Lenti-ORF clone of KDM4B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 4B (KDM4B) |
CNY 13,848.00 |
|
| RC223401L2 | Lenti-ORF clone of KDM4B (mGFP-tagged)-Human lysine (K)-specific demethylase 4B (KDM4B) |
CNY 10,260.00 |
|
| RC223401L3 | Lenti-ORF clone of KDM4B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 4B (KDM4B) |
CNY 13,848.00 |
|
| RC223401L4 | Lenti-ORF clone of KDM4B (mGFP-tagged)-Human lysine (K)-specific demethylase 4B (KDM4B) |
CNY 13,848.00 |
|
| RG223401 | KDM4B (tGFP-tagged) - Human lysine (K)-specific demethylase 4B (KDM4B) |
CNY 13,048.00 |
|
| SC309823 | KDM4B (untagged)-Human lysine (K)-specific demethylase 4B (KDM4B) |
CNY 11,456.00 |
