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SGLT1 (SLC5A1) (NM_000343) Human Tagged ORF Clone
CAT＃: RC221312
- TrueORF®
SLC5A1 (Myc-DDK-tagged)-Human solute carrier family 5 (sodium/glucose cotransporter), member 1 (SLC5A1), transcript variant 1
ORF Plasmid: tGFP
"NM_000343" in other vectors (6)
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CNY 4,944.00
CNY 5,800.00
| Cited in 5 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D22S675; NAGT; SGLT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221312 representing NM_000343
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGTAGCACCTGGAGCCCCAAGACCACCGCGGTCACCCGGCCTGTTGAGACCCACGAGCTCATTC GCAATGCAGCCGATATCTCCATCATCGTTATCTACTTCGTGGTAGTGATGGCCGTCGGACTGTGGGCTAT GTTTTCCACCAATCGTGGGACTGTTGGAGGCTTCTTCCTGGCAGGCCGAAGTATGGTGTGGTGGCCGATT GGAGCCTCCCTCTTTGCTAGTAACATTGGAAGTGGCCACTTTGTGGGGCTGGCCGGGACTGGGGCAGCTT CAGGCATCGCCATTGGAGGCTTTGAATGGAATGCCCTGGTTTTGGTGGTTGTGCTGGGCTGGCTGTTTGT CCCCATCTATATTAAGGCTGGGGTGGTGACAATGCCAGAGTACCTGAGGAAGCGGTTTGGAGGCCAGCGG ATCCAGGTCTACCTTTCCCTTCTGTCCCTGCTGCTCTACATTTTCACCAAGATCTCGGCAGACATCTTCT CGGGGGCCATATTCATCAATCTGGCCTTAGGCCTGAATCTGTATTTAGCCATCTTTCTCTTATTGGCAAT CACTGCCCTTTACACAATTACAGGGGGCCTGGCGGCGGTGATTTACACGGACACCTTGCAGACGGTGATC ATGCTGGTGGGGTCTTTAATCCTGACTGGGTTTGCTTTTCACGAAGTGGGAGGCTATGACGCCTTCATGG AAAAGTACATGAAAGCCATTCCAACCATAGTGTCTGATGGCAACACCACCTTTCAGGAAAAATGCTACAC TCCAAGGGCCGACTCCTTCCACATCTTCCGAGATCCCCTCACGGGAGACCTCCCATGGCCTGGGTTCATC TTTGGGATGTCCATCCTTACCTTGTGGTACTGGTGCACAGATCAGGTCATTGTGCAGCGCTGCCTCTCAG CCAAGAATATGTCTCACGTGAAGGGTGGCTGCATCCTGTGTGGGTATCTAAAGCTGATGCCCATGTTCAT CATGGTGATGCCAGGAATGATCAGCCGCATTCTGTACACAGAAAAAATTGCCTGTGTCGTCCCTTCAGAA TGTGAGAAATATTGCGGTACCAAGGTTGGCTGTACCAACATCGCCTATCCAACCTTAGTGGTGGAGCTCA TGCCCAATGGACTGCGAGGCCTGATGCTATCAGTCATGCTGGCCTCCCTCATGAGCTCCCTGACCTCCAT CTTCAACAGCGCCAGCACCCTCTTCACCATGGACATCTACGCCAAGGTCCGCAAGAGAGCATCTGAGAAA GAGCTCATGATTGCCGGAAGGTTGTTTATCCTGGTGCTGATTGGCATCAGCATCGCCTGGGTGCCCATTG TGCAGTCAGCACAAAGTGGGCAACTCTTCGATTACATCCAGTCCATCACCAGTTACTTGGGACCACCCAT TGCGGCTGTCTTCCTGCTTGCTATTTTCTGGAAGAGAGTCAATGAGCCAGGAGCCTTTTGGGGACTGATC CTAGGACTTCTGATTGGGATTTCACGTATGATTACTGAGTTTGCTTATGGAACCGGGAGCTGCATGGAGC CCAGCAACTGTCCCACGATTATCTGTGGGGTGCACTACTTGTACTTTGCCATTATCCTCTTCGCCATTTC TTTCATCACCATCGTGGTCATCTCCCTCCTCACCAAACCCATTCCGGATGTGCATCTCTACCGTCTGTGT TGGAGCCTGCGCAACAGCAAAGAGGAGCGTATTGACCTGGATGCGGAAGAGGAGAACATCCAAGAAGGCC CTAAGGAGACCATTGAAATAGAAACACAAGTTCCTGAGAAGAAAAAAGGAATCTTCAGGAGAGCCTATGA CCTATTTTGTGGGCTAGAGCAGCACGGTGCACCCAAGATGACTGAGGAAGAGGAGAAAGCCATGAAGATG AAGATGACGGACACCTCTGAGAAGCCTTTGTGGAGGACAGTGTTGAACGTCAATGGCATCATCCTGGTGA CCGTGGCTGTCTTTTGCCATGCATATTTTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221312 representing NM_000343
Red=Cloning site Green=Tags(s) MDSSTWSPKTTAVTRPVETHELIRNAADISIIVIYFVVVMAVGLWAMFSTNRGTVGGFFLAGRSMVWWPI GASLFASNIGSGHFVGLAGTGAASGIAIGGFEWNALVLVVVLGWLFVPIYIKAGVVTMPEYLRKRFGGQR IQVYLSLLSLLLYIFTKISADIFSGAIFINLALGLNLYLAIFLLLAITALYTITGGLAAVIYTDTLQTVI MLVGSLILTGFAFHEVGGYDAFMEKYMKAIPTIVSDGNTTFQEKCYTPRADSFHIFRDPLTGDLPWPGFI FGMSILTLWYWCTDQVIVQRCLSAKNMSHVKGGCILCGYLKLMPMFIMVMPGMISRILYTEKIACVVPSE CEKYCGTKVGCTNIAYPTLVVELMPNGLRGLMLSVMLASLMSSLTSIFNSASTLFTMDIYAKVRKRASEK ELMIAGRLFILVLIGISIAWVPIVQSAQSGQLFDYIQSITSYLGPPIAAVFLLAIFWKRVNEPGAFWGLI LGLLIGISRMITEFAYGTGSCMEPSNCPTIICGVHYLYFAIILFAISFITIVVISLLTKPIPDVHLYRLC WSLRNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKM KMTDTSEKPLWRTVLNVNGIILVTVAVFCHAYFA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000343 |
| ORF Size | 1992 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000343.4 |
| RefSeq Size | 2449 bp |
| RefSeq ORF | 1995 bp |
| Locus ID | 6523 |
| UniProt ID | P13866 |
| Domains | SSF |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 73.3 kDa |
| Gene Summary | This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Pharmacological evaluation of HM41322, a novel SGLT1/2 dual inhibitor, in vitro and in vivo
,Lee, KH;Lee, SD;Kim, N;Suh, KH;Kim, YH;Sim, SS;,
Korean J. Physiol. Pharmacol.
,PubMed ID 30627010
[SGLT1]
|
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Concise and Stereodivergent Synthesis of Carbasugars Reveals Unexpected Structure-Activity Relationship (SAR) of SGLT2 Inhibition
,Ng, WL;Li, HC;Lau, KM;Chan, AKN;Lau, CB;Shing, TKM;,
Sci Rep
,PubMed ID 28717146
[SGLT1]
|
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Targeting Type 2 Diabetes with C-Glucosyl Dihydrochalcones as Selective Sodium Glucose Co-Transporter 2 (SGLT2) Inhibitors: Synthesis and Biological Evaluation
,Jesus, AR;Vila-Viçosa, D;Machuqueiro, M;Marques, AP;Dore, TM;Rauter, AP;,
Journal of Medicinal Chemistry
,PubMed ID 28098449
[SGLT1]
|
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The design and synthesis of novel SGLT2 inhibitors: C-glycosides with benzyltriazolopyridinone and phenylhydantoin as the aglycone moieties
,Guo, C;Hu, M;DeOrazio, RJ;Usyatinsky, A;Fitzpatrick, K;Zhang, Z;Maeng, JH;Kitchen, DB;Tom, S;Luche, M;Khmelnitsky, Y;Mhyre, AJ;Guzzo, PR;Liu, S;,
Bioorg. Med. Chem. Jul 2014
,PubMed ID 24842618
[SGLT1]
|
|
SHR3824, a novel selective inhibitor of renal sodium glucose cotransporter 2, exhibits antidiabetic efficacy in rodent models
,Yan, PK;Zhang, LN;Feng, Y;Qu, H;Qin, L;Zhang, LS;Leng, Y;,
Acta Pharmacol. Sin.
,PubMed ID 24786232
[SGLT1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221312L1 | Lenti ORF clone of Human solute carrier family 5 (sodium/glucose cotransporter), member 1 (SLC5A1), transcript variant 1, Myc-DDK-tagged |
CNY 7,344.00 |
|
| RC221312L2 | Lenti ORF clone of Human solute carrier family 5 (sodium/glucose cotransporter), member 1 (SLC5A1), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC221312L3 | Lenti ORF clone of Human solute carrier family 5 (sodium/glucose cotransporter), member 1 (SLC5A1), transcript variant 1, Myc-DDK-tagged |
CNY 7,344.00 |
|
| RC221312L4 | Lenti ORF clone of Human solute carrier family 5 (sodium/glucose cotransporter), member 1 (SLC5A1), transcript variant 1, mGFP tagged |
CNY 7,344.00 |
|
| RG221312 | SLC5A1 (tGFP-tagged) - Human solute carrier family 5 (sodium/glucose cotransporter), member 1 (SLC5A1), transcript variant 1 |
CNY 6,370.00 |
|
| SC119918 | SLC5A1 (untagged)-Human solute carrier family 5 (sodium/glucose cotransporter), member 1 (SLC5A1), transcript variant 1 |
CNY 4,952.00 |
