LAMP2 (NM_002294) Human Tagged ORF Clone
CAT#: RC221216
LAMP2 (Myc-DDK-tagged)-Human lysosomal-associated membrane protein 2 (LAMP2), transcript variant A
ORF Plasmid: tGFP
"NM_002294" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
Cited in 6 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CD107b; DND; LAMP-2; LAMPB; LGP-96; LGP110 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC221216 representing NM_002294
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTG TGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCA GATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGC ACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGAC CTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTC CTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTT TTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATG TTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTT CCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACA ACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGC TGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAA TACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAG TATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGT ATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAG TTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTA AGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACA ACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTT TATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221216 representing NM_002294
Red=Cloning site Green=Tags(s) MVCFRLFPVPGSGLVLVCLVLGAVRSYALELNLTDSENATCLYAKWQMNFTVRYETTNKTYKTVTISDHG TVTYNGSICGDDQNGPKIAVQFGPGFSWIANFTKAASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDEL LAIRIPLNDLFRCNSLSTLEKNDVVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPTIHTTVPSPTT TPTPKEKPEAGTYSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTGSCRSHTALLRLNSSTIK YLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDL RVQPFNVTQGKYSTAQDCSADDDNFLVPIAVGAALAGVLILVLLAYFIGLKHHHAGYEQF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002294 |
ORF Size | 1230 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002294.3 |
RefSeq Size | 1868 bp |
RefSeq ORF | 1233 bp |
Locus ID | 3920 |
UniProt ID | P13473 |
Domains | Lamp |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
Protein Pathways | Lysosome |
MW | 44.96 kDa |
Gene Summary | The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008] |
Citations (6)
The use of this cDNA Clones has been cited in the following citations: |
---|
SNX10-mediated degradation of LAMP2A by NSAIDs inhibits chaperone-mediated autophagy and induces hepatic lipid accumulation
,null,
Theranostics
,PubMed ID 35265214
[LAMP2]
|
Direct homophilic interaction of LAMP2A with the two-domain architecture revealed by site-directed photo-crosslinks and steric hindrances in mammalian cells
,Terasawa, K;Kato, Y;Ikami, Y;Sakamoto, K;Ohtake, K;Kusano, S;Tomabechi, Y;Kukimoto-Niino, M;Shirouzu, M;Guan, JL;Kobayashi, T;Iwata, T;Watabe, T;Yokoyama, S;Hara-Yokoyama, M;,
Autophagy
,PubMed ID 33849387
[LAMP2]
|
Splice variants of lysosome-associated membrane proteins 2A and 2B are involved in sunitinib resistance in human renal cell carcinoma cells
,null,
Oncology Reports
,PubMed ID 32901843
[LAMP2]
|
Chaperone-Mediated Autophagy Suppresses Apoptosis via Regulation of the Unfolded Protein Response during Chronic Obstructive Pulmonary Disease Pathogenesis
,Hosaka, Y;Araya, J;Fujita, Y;Kadota, T;Tsubouchi, K;Yoshida, M;Minagawa, S;Hara, H;Kawamoto, H;Watanabe, N;Ito, A;Ichikawa, A;Saito, N;Okuda, K;Watanabe, J;Takekoshi, D;Utsumi, H;Hashimoto, M;Wakui, H;Ito, S;Numata, T;Mori, S;Matsudaira, H;Hirano, J;Ohtsuka, T;Nakayama, K;Kuwano, K;,
J. Immunol.
,PubMed ID 32699159
[LAMP2]
|
Phosphorylation of LAMP2A by p38 MAPK couples ER stress to chaperone-mediated autophagy
,Li, W;Zhu, J;Dou, J;She, H;Tao, K;Xu, H;Yang, Q;Mao, Z;,
Nat Commun
,PubMed ID 29176575
[LAMP2]
|
Chronic acidosis in the tumour microenvironment selects for overexpression of LAMP2 in the plasma membrane
,Damaghi, M;Tafreshi, NK;Lloyd, MC;Sprung, R;Estrella, V;Wojtkowiak, JW;Morse, DL;Koomen, JM;Bui, MM;Gatenby, RA;Gillies, RJ;,
Nat Commun
,PubMed ID 26658462
[LAMP2]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221216L1 | Lenti ORF clone of Human lysosomal-associated membrane protein 2 (LAMP2), transcript variant A, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC221216L2 | Lenti ORF clone of Human lysosomal-associated membrane protein 2 (LAMP2), transcript variant A, mGFP tagged |
CNY 5,890.00 |
|
RC221216L3 | Lenti ORF clone of Human lysosomal-associated membrane protein 2 (LAMP2), transcript variant A, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC221216L4 | Lenti ORF clone of Human lysosomal-associated membrane protein 2 (LAMP2), transcript variant A, mGFP tagged |
CNY 5,890.00 |
|
RG221216 | LAMP2 (tGFP-tagged) - Human lysosomal-associated membrane protein 2 (LAMP2), transcript variant A |
CNY 4,370.00 |
|
SC118738 | LAMP2 (untagged)-Human lysosomal-associated membrane protein 2 (LAMP2), transcript variant A |
CNY 3,656.00 |