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Plexin A2 (PLXNA2) (NM_025179) Human Tagged ORF Clone
CAT＃: RC221024
- TrueORF®
PLXNA2 (Myc-DDK-tagged)-Human plexin A2 (PLXNA2)
ORF Plasmid: tGFP
"NM_025179" in other vectors (3)
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CNY 19,096.00
货期*
现货
规格
| Cited in 6 publications. |
Product images
经常一起买 (4)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | OCT; PLXN2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221024 representing NM_025179
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACAGAGGCGGCCCTGGCCCCGGGCCCTGGAGGTGGACAGCCGCTCTGTGGTCCTGCTCTCAGTGG TCTGGGTGCTGCTGGCCCCCCCAGCAGCCGGCATGCCTCAGTTCAGCACCTTCCACTCTGAGAATCGTGA CTGGACCTTCAACCACTTGACCGTCCACCAAGGGACGGGGGCCGTCTATGTGGGGGCCATCAACCGGGTC TATAAGCTGACAGGCAACCTGACCATCCAGGTGGCTCATAAGACAGGGCCAGAAGAGGACAACAAGTCTT GTTACCCGCCCCTCATCGTGCAGCCCTGCAGCGAAGTGCTCACCCTCACCAACAATGTCAACAAGCTGCT CATCATTGACTACTCTGAGAACCGCCTGCTGGCCTGTGGGAGCCTCTACCAGGGGGTCTGCAAGCTGCTG CGGCTGGATGACCTCTTCATCCTGGTGGAGCCATCCCACAAGAAGGAGCACTACCTGTCCAGTGTCAACA AGACGGGCACCATGTACGGGGTGATTGTGCGCTCTGAGGGTGAGGATGGCAAGCTCTTCATCGGCACGGC TGTGGATGGGAAGCAGGATTACTTCCCGACCCTGTCCAGCCGGAAGCTGCCCCGAGACCCTGAGTCCTCA GCCATGCTCGACTATGAGCTACACAGCGATTTTGTCTCCTCTCTCATCAAGATCCCTTCAGACACCCTGG CCCTGGTCTCCCACTTTGACATCTTCTACATCTACGGCTTTGCTAGTGGGGGCTTTGTCTACTTTCTCAC TGTCCAGCCCGAGACCCCTGAGGGTGTGGCCATCAACTCCGCTGGAGACCTCTTCTACACCTCACGCATC GTGCGGCTCTGCAAGGATGACCCCAAGTTCCACTCATACGTGTCCCTGCCCTTCGGCTGCACCCGGGCCG GGGTGGAATACCGCCTCCTGCAGGCTGCTTACCTGGCCAAGCCTGGGGACTCACTGGCCCAGGCCTTCAA TATCACCAGCCAGGACGATGTACTCTTTGCCATCTTCTCCAAAGGGCAGAAGCAGTATCACCACCCGCCC GATGACTCTGCCCTGTGTGCCTTCCCTATCCGGGCCATCAACTTGCAGATCAAGGGGCGCCTACAGTCCT GCTACCAGGGCGAGGGCAACCTGGAGCTCAACTGGCTGCTGGGGAAGGACGTCCAGTGCACCAAGGCGCC TGTCCCCATCGATGATAACTTCTGTGGACTGGACATCAACCAGCCCCTGGGAGGCTCAACTCCAGTGGAG GGCCTGACCCTGTACACCACCAGCAGGGACCGCATGACCTCTGTGGCCTCCTACGTTTACAACGGCTACA GCGTGGTTTTTGTGGGGACTAAGAGTGGCAAGCTGAAAAAGATTCGGGCCGACGGTCCCCCCCATGGTGG GGTCCAGTACGAGATGGTCTCTGTGCTCAAGGACGGAAGCCCCATCCTCCGGGACATGGCCTTCTCCATT GATCAGCGCTACCTGTACGTCATGTCTGAGAGACAGGTCACCAGGGTCCCCGTGGAGTCATGTGAGCAGT ATACGACTTGTGGGGAGTGCCTGAGCTCTGGGGACCCTCACTGTGGCTGGTGTGCCCTGCACAACATGTG CTCCCGCAGGGACAAATGCCAACAGGCCTGGGAACCTAATCGATTTGCTGCCAGCATCAGCCAGTGTGTG AGCCTTGCAGTGCATCCCAGCAGCATCTCAGTATCTGAGCACAGCCGGTTGCTTAGCCTGGTAGTGAGTG ATGCTCCTGATCTATCTGCGGGTATCGCCTGTGCCTTTGGGAACCTGACAGAGGTGGAGGGGCAGGTGTC CGGGAGCCAGGTCATCTGCATCTCACCTGGGCCCAAGGATGTCCCTGTCATCCCGCTGGATCAAGACTGG TTTGGGCTGGAGCTACAGCTGAGGTCCAAGGAGACAGGGAAGATATTTGTCAGCACCGAGTTCAAGTTTT ACAACTGCAGTGCCCACCAACTGTGCCTGTCCTGTGTCAACAGCGCCTTCCGCTGCCATTGGTGCAAGTA CCGCAACCTCTGCACTCATGACCCCACCACCTGCTCCTTCCAGGAGGGCCGGATCAATATTTCAGAGGAC TGTCCCCAGCTGGTGCCCACAGAGGAGATCTTGATTCCAGTCGGGGAGGTAAAGCCAATCACCCTTAAGG CGCGAAATCTGCCCCAGCCGCAGTCCGGCCAGCGAGGCTATGAGTGTGTCCTCAACATACAAGGAGCCAT CCACCGGGTCCCCGCTCTGCGCTTCAACAGCTCCAGCGTTCAGTGTCAGAACAGCTCGTACCAGTATGAT GGCATGGACATCAGCAATCTGGCCGTGGATTTCGCTGTGGTGTGGAACGGCAATTTCATCATTGACAACC CTCAGGACCTGAAAGTCCATCTCTACAAGTGTGCAGCCCAGCGGGAGAGCTGCGGCCTCTGCCTCAAGGC CGACCGGAAGTTTGAGTGTGGCTGGTGCAGCGGCGAGCGCAGGTGCACCCTCCACCAGCACTGTACCAGC CCTTCCAGCCCCTGGCTCGACTGGTCCAGCCACAATGTCAAGTGCTCCAACCCTCAAATCACCGAGATTT TGACGGTGTCTGGACCGCCGGAAGGAGGGACGCGAGTGACCATCCATGGCGTGAACCTGGGTCTGGACTT CTCCGAGATCGCCCACCATGTGCAGGTGGCTGGGGTGCCCTGCACGCCCCTCCCAGGGGAATACATCATC GCTGAGCAGATTGTCTGTGAGATGGGCCATGCCCTCGTGGGAACCACCTCCGGGCCAGTACGCCTGTGTA TTGGCGAGTGTAAGCCAGAGTTCATGACGAAGTCCCATCAGCAGTACACCTTCGTGAACCCTTCTGTGCT GTCACTCAACCCAATCCGAGGTCCCGAGTCAGGAGGCACTATGGTGACCATTACCGGCCATTACCTTGGG GCTGGGAGCAGCGTGGCAGTCTACCTGGGCAACCAGACCTGCGAGTTCTACGGGAGGTCAATGAGTGAGA TCGTGTGTGTCTCACCCCCATCATCCAATGGCCTTGGCCCGGTCCCTGTTTCTGTGAGTGTCGACCGAGC CCATGTGGATAGCAACCTGCAGTTTGAGTACATAGATGACCCTCGGGTCCAGCGCATCGAGCCAGAGTGG AGCATTGCCAGTGGCCACACACCCCTGACCATCACAGGCTTCAACCTGGATGTCATTCAGGAGCCAAGGA TCCGAGTCAAATTCAATGGCAAAGAATCTGTCAATGTGTGTAAAGTTGTGAACACAACCACCCTCACCTG CCTGGCACCCTCTCTGACCACGGACTACCGCCCTGGCCTGGACACTGTGGAACGCCCAGATGAGTTTGGA TTTGTCTTTAACAATGTCCAATCCTTGCTAATTTACAACGACACCAAGTTTATCTACTACCCCAACCCGA CCTTTGAACTGCTTAGCCCTACTGGAGTCTTGGATCAAAAGCCAGGATCGCCCATCATTCTGAAGGGCAA AAACCTCTGCCCTCCTGCCTCTGGAGGGGCCAAACTCAACTACACTGTGCTCATCGGAGAGACCCCTTGT GCTGTCACCGTATCTGAGACCCAGCTTCTCTGCGAGCCTCCCAACCTCACCGGGCAGCACAAGGTCATGG TTCACGTGGGCGGGATGGTGTTCTCGCCTGGCTCGGTGAGTGTCATCTCAGACAGCTTGCTGACCCTGCC AGCCATCGTCAGCATCGCGGCCGGCGGCAGCCTCCTCCTCATCATCGTCATCATCGTCCTCATTGCCTAC AAGCGCAAGTCTCGAGAAAATGACCTCACTCTCAAGCGGCTGCAAATGCAGATGGACAATCTGGAGTCCC GTGTGGCCTTGGAGTGCAAGGAAGCTTTTGCTGAGCTCCAGACGGATATCAATGAGTTGACCAGTGACCT GGACCGCTCAGGAATCCCTTACCTGGACTATCGTACCTACGCTATGCGAGTCCTGTTCCCGGGCATCGAG GACCACCCCGTCCTGCGGGAGCTGGAGGTACAAGGAAACGGGCAGCAGCACGTGGAGAAGGCCCTGAAGC TCTTTGCCCAGCTCATCAACAACAAGGTGTTCCTGCTGACCTTCATCCGCACCCTGGAGCTGCAGCGCAG TTTCTCCATGCGCGACCGGGGCAACGTGGCTTCGCTCATCATGACCGGCCTGCAGGGCCGCCTGGAATAT GCCACTGATGTCCTCAAGCAGCTGCTCTCTGACCTCATCGATAAGAACCTGGAGAACAAGAACCACCCCA AGCTGCTACTCCGGAGGACAGAGTCTGTGGCTGAAAAGATGCTGACCAATTGGTTCGCCTTCCTCCTGCA CAAGTTCCTAAAGGAGTGCGCAGGGGAGCCACTCTTCATGCTATACTGTGCCATCAAGCAGCAGATGGAG AAGGGCCCCATTGATGCCATCACGGGCGAGGCCCGCTACTCCCTGAGCGAGGACAAGCTCATCCGGCAGC AGATCGAGTACAAGACCCTGATCCTGAACTGCGTCAACCCTGACAACGAGAACAGTCCAGAGATCCCAGT GAAGGTGTTAAACTGTGACACCATCACACAGGTCAAGGAGAAGATTCTTGATGCCGTGTATAAGAATGTG CCCTATTCCCAGCGGCCGAGGGCAGTGGACATGGACTTGGAGTGGCGCCAAGGCCGGATCGCCCGGGTCG TGCTGCAAGATGAGGACATCACCACCAAGATTGAGGGTGACTGGAAGCGGCTCAACACACTGATGCATTA TCAGGTGTCAGACAGGTCGGTGGTGGCTCTGGTCCCCAAACAGACCTCCTCCTACAACATCCCTGCCTCT GCCAGCATCTCCCGGACGTCCATCAGCAGATATGACTCCTCCTTCAGGTATACGGGCAGCCCCGACAGCC TGCGGTCCCGGGCCCCGATGATCACCCCAGACCTGGAAAGTGGGGTCAAGGTGTGGCATCTGGTGAAGAA CCATGACCACGGTGACCAGAAGGAGGGTGACCGGGGCAGCAAGATGGTGTCCGAGATCTACCTGACCCGG CTACTGGCCACCAAGGGCACCCTGCAGAAGTTTGTGGACGACTTGTTTGAGACCTTGTTCAGCACTGTGC ACCGGGGCAGCGCTCTCCCCCTGGCCATCAAGTACATGTTTGATTTCCTAGATGAGCAGGCAGACAGGCA CAGCATCCATGACACAGATGTGCGGCACACCTGGAAAAGCAACTGCCTCCCTCTGCGCTTCTGGGTGAAC GTGATTAAGAACCCCCAGTTCGTGTTTGACATCCACAAGGGCAGCATCACGGACGCCTGCCTCTCTGTGG TGGCCCAGACCTTCATGGACTCTTGTTCAACGTCAGAGCACCGGCTGGGCAAGGACTCCCCCTCCAACAA GCTGCTCTATGCCAAGGACATCCCCAGCTACAAGAGCTGGGTGGAGAGATACTACGCAGACATCGCCAAG CTCCCAGCCATCAGTGACCAGGACATGAATGCCTACCTCGCCGAGCAGTCCCGCCTGCACGCCGTGGAGT TCAACATGCTGAGTGCCCTCAATGAGATCTACTCCTATGTCAGCAAGTATAGTGAGGAGCTCATCGGGGC CCTAGAGCAGGATGAGCAGGCACGGCGGCAGCGGCTGGCTTATAAGGTGGAGCAGCTCATTAATGCCATG TCCATTGAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221024 representing NM_025179
Red=Cloning site Green=Tags(s) MEQRRPWPRALEVDSRSVVLLSVVWVLLAPPAAGMPQFSTFHSENRDWTFNHLTVHQGTGAVYVGAINRV YKLTGNLTIQVAHKTGPEEDNKSCYPPLIVQPCSEVLTLTNNVNKLLIIDYSENRLLACGSLYQGVCKLL RLDDLFILVEPSHKKEHYLSSVNKTGTMYGVIVRSEGEDGKLFIGTAVDGKQDYFPTLSSRKLPRDPESS AMLDYELHSDFVSSLIKIPSDTLALVSHFDIFYIYGFASGGFVYFLTVQPETPEGVAINSAGDLFYTSRI VRLCKDDPKFHSYVSLPFGCTRAGVEYRLLQAAYLAKPGDSLAQAFNITSQDDVLFAIFSKGQKQYHHPP DDSALCAFPIRAINLQIKGRLQSCYQGEGNLELNWLLGKDVQCTKAPVPIDDNFCGLDINQPLGGSTPVE GLTLYTTSRDRMTSVASYVYNGYSVVFVGTKSGKLKKIRADGPPHGGVQYEMVSVLKDGSPILRDMAFSI DQRYLYVMSERQVTRVPVESCEQYTTCGECLSSGDPHCGWCALHNMCSRRDKCQQAWEPNRFAASISQCV SLAVHPSSISVSEHSRLLSLVVSDAPDLSAGIACAFGNLTEVEGQVSGSQVICISPGPKDVPVIPLDQDW FGLELQLRSKETGKIFVSTEFKFYNCSAHQLCLSCVNSAFRCHWCKYRNLCTHDPTTCSFQEGRINISED CPQLVPTEEILIPVGEVKPITLKARNLPQPQSGQRGYECVLNIQGAIHRVPALRFNSSSVQCQNSSYQYD GMDISNLAVDFAVVWNGNFIIDNPQDLKVHLYKCAAQRESCGLCLKADRKFECGWCSGERRCTLHQHCTS PSSPWLDWSSHNVKCSNPQITEILTVSGPPEGGTRVTIHGVNLGLDFSEIAHHVQVAGVPCTPLPGEYII AEQIVCEMGHALVGTTSGPVRLCIGECKPEFMTKSHQQYTFVNPSVLSLNPIRGPESGGTMVTITGHYLG AGSSVAVYLGNQTCEFYGRSMSEIVCVSPPSSNGLGPVPVSVSVDRAHVDSNLQFEYIDDPRVQRIEPEW SIASGHTPLTITGFNLDVIQEPRIRVKFNGKESVNVCKVVNTTTLTCLAPSLTTDYRPGLDTVERPDEFG FVFNNVQSLLIYNDTKFIYYPNPTFELLSPTGVLDQKPGSPIILKGKNLCPPASGGAKLNYTVLIGETPC AVTVSETQLLCEPPNLTGQHKVMVHVGGMVFSPGSVSVISDSLLTLPAIVSIAAGGSLLLIIVIIVLIAY KRKSRENDLTLKRLQMQMDNLESRVALECKEAFAELQTDINELTSDLDRSGIPYLDYRTYAMRVLFPGIE DHPVLRELEVQGNGQQHVEKALKLFAQLINNKVFLLTFIRTLELQRSFSMRDRGNVASLIMTGLQGRLEY ATDVLKQLLSDLIDKNLENKNHPKLLLRRTESVAEKMLTNWFAFLLHKFLKECAGEPLFMLYCAIKQQME KGPIDAITGEARYSLSEDKLIRQQIEYKTLILNCVNPDNENSPEIPVKVLNCDTITQVKEKILDAVYKNV PYSQRPRAVDMDLEWRQGRIARVVLQDEDITTKIEGDWKRLNTLMHYQVSDRSVVALVPKQTSSYNIPAS ASISRTSISRYDSSFRYTGSPDSLRSRAPMITPDLESGVKVWHLVKNHDHGDQKEGDRGSKMVSEIYLTR LLATKGTLQKFVDDLFETLFSTVHRGSALPLAIKYMFDFLDEQADRHSIHDTDVRHTWKSNCLPLRFWVN VIKNPQFVFDIHKGSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIPSYKSWVERYYADIAK LPAISDQDMNAYLAEQSRLHAVEFNMLSALNEIYSYVSKYSEELIGALEQDEQARRQRLAYKVEQLINAM SIES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025179 |
| ORF Size | 5682 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025179.2, NP_079455.2 |
| RefSeq Size | 6263 bp |
| RefSeq ORF | 5685 bp |
| Locus ID | 5362 |
| UniProt ID | O75051 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Axon guidance |
| MW | 210.9 kDa |
| Gene Summary | This gene encodes a member of the plexin-A family of semaphorin co-receptors. Semaphorins are a large family of secreted or membrane-bound proteins that mediate repulsive effects on axon pathfinding during nervous system development. A subset of semaphorins are recognized by plexin-A/neuropilin transmembrane receptor complexes, triggering a cellular signal transduction cascade that leads to axon repulsion. This plexin-A family member is thought to transduce signals from semaphorin-3A and -3C. [provided by RefSeq, Jul 2008] |
Citations (6)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Novel Truncating and Missense Variants in SEMA6B in Patients With Early-Onset Epilepsy
,Xiaozhen, S;Fan, Y;Fang, Y;Xiaoping, L;Jia, J;Wuhen, X;Xiaojun, T;Jun, S;Yucai, C;Hong, Z;Guang, H;Shengnan, W;,
Frontiers in cell and developmental biology
,PubMed ID 34017830
[Plexin A2]
|
|
Shootin-1 Is Required for Nervous System Development in Zebrafish
,Emerson, SE;Stergas, HR;Bupp-Chickering, SO;Ebert, AM;,
Dev. Dyn.
,PubMed ID 32406957
[Plexin A2]
|
|
Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance
,null,
Cell
,PubMed ID 30661757
[Plexin A2]
|
|
Fyn-dependent phosphorylation of PlexinA1 and PlexinA2 at conserved tyrosines is essential for zebrafish eye development
,St Clair, RM;Emerson, SE;D'Elia, KP;Weir, ME;Schmoker, AM;Ebert, AM;Ballif, BA;,
FEBS J.2017
,PubMed ID 29091353
[Plexin A2]
|
|
Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish
,Emerson, SE;Clair, RMS;Waldron, AL;Bruno, SR;Duong, A;Driscoll, HE;Ballif, BA;McFarlane, S;Ebert, AM;,
Dev. Dyn.
,PubMed ID 28440030
[Plexin A2]
|
|
Sema6a and Plxna2 mediate spatially regulated repulsion within the developing eye to promote eye vesicle cohesion
,Ebert, AM;Childs, SJ;Hehr, CL;Cechmanek, PB;McFarlane, S;,
Development
,PubMed ID 24917502
[Plexin A2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221024L1 | Lenti ORF clone of Human plexin A2 (PLXNA2), Myc-DDK-tagged |
CNY 21,496.00 |
|
| RG221024 | PLXNA2 (tGFP-tagged) - Human plexin A2 (PLXNA2) |
CNY 20,810.00 |
|
| SC308779 | PLXNA2 (untagged)-Human plexin A2 (PLXNA2) |
CNY 26,790.00 |
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