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Mannosidase II (MAN2A1) (NM_002372) Human Tagged ORF Clone
CAT＃: RC220186
MAN2A1 (Myc-DDK-tagged)-Human mannosidase, alpha, class 2A, member 1 (MAN2A1)
ORF Plasmid: tGFP
"NM_002372" in other vectors (6)
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CNY 7,968.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AMan II; GOLIM7; MANA2; MANII |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220186 representing NM_002372
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTAAGCCGCCAGTTCACCGTGTTCGGCAGTGCGATCTTCTGTGTGGTGATTTTCTCGCTCTACC TGATGCTGGACCGGGGTCACTTAGACTACCCCAGGAACCCGCGCCGCGAGGGCTCCTTCCCTCAGGGCCA GCTCTCAATGTTGCAAGAAAAAATAGACCATTTGGAGCGTTTGCTAGCTGAGAATAATGAGATCATCTCA AATATTAGAGACTCAGTCATCAATTTGAGTGAGTCTGTGGAGGATGGTCCGAAAAGTTCACAAAGCAATT TCAGCCAAGGTGCTGGCTCACATCTTCTGCCCTCACAATTATCCCTCTCAGTTGACACTGCAGACTGTCT GTTTGCTTCACAAAGTGGAAGTCACAATTCAGATGTGCAGATGTTGGATGTTTACAGTCTAATTTCTTTT GACAATCCAGATGGTGGAGTTTGGAAGCAAGGATTTGACATTACTTATGAATCTAATGAATGGGACACTG AACCCCTTCAAGTCTTTGTGGTGCCTCATTCCCATAACGACCCAGGTTGGTTGAAGACTTTCAATGACTA CTTTAGAGACAAGACTCAGTATATTTTTAATAACATGGTCCTAAAGCTGAAAGAAGACTCACGGAGGAAG TTTATTTGGTCTGAGATCTCTTACCTTTCAAAGTGGTGGGATATTATAGATATTCAGAAGAAGGATGCTG TTAAAAGTTTAATAGAAAATGGTCAGCTTGAAATTGTGACAGGTGGCTGGGTTATGCCTGATGAAGCTAC TCCACATTATTTTGCCTTAATTGATCAACTAATTGAAGGACATCAGTGGCTGGAAAATAATATAGGAGTG AAACCTCGGTCCGGCTGGGCTATTGATCCCTTTGGACACTCACCAACAATGGCTTATCTTCTAAACCGTG CTGGACTTTCTCACATGCTTATCCAGAGAGTTCATTATGCAGTTAAAAAACACTTTGCACTGCATAAAAC ATTGGAGTTTTTTTGGAGACAGAATTGGGATCTGGGATCTGTCACAGATATTTTATGCCACATGATGCCC TTCTACAGCTATGACATCCCTCACACTTGTGGACCTGATCCTAAAATATGCTGCCAGTTTGATTTTAAAC GTCTTCCTGGAGGCAGATTTGGTTGTCCCTGGGGAGTCCCCCCAGAAACAATACATCCTGGAAATGTCCA AAGCAGGGCTCGGATGCTACTAGATCAGTACCGAAAGAAGTCAAAGCTTTTTCGAACCAAAGTTCTCCTG GCTCCACTAGGAGATGATTTCCGCTACTGTGAATACACGGAATGGGATTTACAGTTTAAGAATTATCAGC AGCTTTTTGATTATATGAATTCTCAGTCCAAGTTTAAAGTTAAGATGCAGTTTGGAACTTTATCAGATTT TTTTGATGCGCTGGATAAAGCAGATGAAACTCAGAGAGACAAGGGCCAATCGATGTTCCCTGTTTTAAGT GGAGATTTTTTCACTTATGCCGATCGAGATGATCATTACTGGAGTGGCTATTTTACATCCAGACCCTTTT ACAAACGAATGGACAGAATCATGGAATCTCATTTAAGGGCTGCTGAAATTCTTTACTATTTCGCCCTGAG ACAAGCTCACAAATACAAGATAAATAAATTTCTCTCATCATCACTTTACACGGCACTGACAGAAGCCAGA AGGAATTTGGGACTGTTTCAACATCATGATGCTATCACAGGAACTGCAAAAGACTGGGTGGTTGTGGATT ATGGTACCAGACTTTTTCATTCGTTAATGGTTTTGGAGAAGATAATTGGAAATTCTGCATTTCTTCTTAT TTTGAAGGACAAACTCACATACGACTCTTACTCTCCTGATACCTTCCTGGAGATGGATTTGAAACAAAAA TCACAAGATTCTCTGCCACAAAAAAATATAATAAGGCTGAGTGCGGAGCCAAGGTACCTTGTGGTCTATA ATCCTTTAGAACAAGACCGAATCTCGTTGGTCTCAGTCTATGTGAGTTCCCCGACAGTGCAAGTGTTCTC TGCTTCAGGAAAACCTGTGGAAGTTCAAGTCAGCGCAGTTTGGGATACAGCAAATACTATTTCAGAAACA GCCTATGAGATCTCTTTTCGAGCACATATACCGCCATTGGGACTGAAAGTGTATAAGATTTTGGAATCAG CAAGTTCAAATTCACATTTAGCTGATTATGTCTTGTATAAGAATAAAGTAGAAGATAGCGGAATTTTCAC CATAAAGAATATGATAAATACTGAAGAAGGTATAACACTAGAGAACTCCTTTGTTTTACTTCGGTTTGAT CAAACTGGACTTATGAAGCAAATGATGACTAAAGAAGATGGTAAACACCATGAAGTAAATGTGCAATTTT CATGGTATGGAACCACAATTAAAAGAGACAAAAGTGGTGCCTACCTCTTCTTACCTGATGGTAATGCCAA GCCTTATGTTTACACAACACCGCCCTTTGTCAGAGTGACACATGGAAGGATTTATTCGGAAGTGACTTGC TTTTTTGACCATGTTACTCATAGAGTCCGACTATACCACATACAGGGAATAGAAGGACAGTCTGTGGAAG TTTCCAATATTGTGGACATCCGAAAAGTATATAACCGTGAGATTGCAATGAAAATTTCTTCTGATATAAA AAGCCAAAATAGATTTTATACTGACCTAAATGGGTACCAGATTCAACCTAGAATGACACTGAGCAAATTG CCTCTTCAAGCAAATGTCTATCCCATGACCACAATGGCCTATATCCAGGATGCCAAACATCGTTTGACAC TGCTCTCTGCTCAGTCTTTAGGGGTTTCGAGTTTGAATAGTGGTCAGATTGAAGTTATCATGGATCGAAG ACTCATGCAAGATGATAATCGTGGCCTTGAGCAAGGTATCCAGGATAACAAGATTACAGCTAATCTATTT CGAATACTACTAGAAAAAAGAAGTGCTGTTAATACGGAAGAAGAAAAGAAGTCGGTCAGTTATCCTTCTC TCCTTAGCCACATAACTTCTTCTCTCATGAATCATCCAGTCATTCCAATGGCAAATAAGTTCTCCTCACC TACCCTTGAGCTGCAAGGTGAATTCTCTCCATTACAGTCATCTTTGCCTTGTGACATTCATCTGGTTAAT TTGAGAACAATACAGTCAAAGGTGGGCAATGGGCACTCCAATGAGGCAGCCTTGATCCTCCACAGAAAAG GGTTTGATTGTCGGTTCTCTAGCAAAGGCACAGGGCTGTTTTGTTCTACTACTCAGGGAAAGATATTGGT ACAGAAACTTTTAAACAAGTTTATTGTCGAAAGTCTCACACCTTCATCACTATCCTTGATGCATTCACCT CCCGGCACTCAGAATATAAGTGAGATCAACTTGAGTCCAATGGAAATCAGCACATTCCGAATCCAGTTGA GG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220186 representing NM_002372
Red=Cloning site Green=Tags(s) MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPRNPRREGSFPQGQLSMLQEKIDHLERLLAENNEIIS NIRDSVINLSESVEDGPKSSQSNFSQGAGSHLLPSQLSLSVDTADCLFASQSGSHNSDVQMLDVYSLISF DNPDGGVWKQGFDITYESNEWDTEPLQVFVVPHSHNDPGWLKTFNDYFRDKTQYIFNNMVLKLKEDSRRK FIWSEISYLSKWWDIIDIQKKDAVKSLIENGQLEIVTGGWVMPDEATPHYFALIDQLIEGHQWLENNIGV KPRSGWAIDPFGHSPTMAYLLNRAGLSHMLIQRVHYAVKKHFALHKTLEFFWRQNWDLGSVTDILCHMMP FYSYDIPHTCGPDPKICCQFDFKRLPGGRFGCPWGVPPETIHPGNVQSRARMLLDQYRKKSKLFRTKVLL APLGDDFRYCEYTEWDLQFKNYQQLFDYMNSQSKFKVKMQFGTLSDFFDALDKADETQRDKGQSMFPVLS GDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESHLRAAEILYYFALRQAHKYKINKFLSSSLYTALTEAR RNLGLFQHHDAITGTAKDWVVVDYGTRLFHSLMVLEKIIGNSAFLLILKDKLTYDSYSPDTFLEMDLKQK SQDSLPQKNIIRLSAEPRYLVVYNPLEQDRISLVSVYVSSPTVQVFSASGKPVEVQVSAVWDTANTISET AYEISFRAHIPPLGLKVYKILESASSNSHLADYVLYKNKVEDSGIFTIKNMINTEEGITLENSFVLLRFD QTGLMKQMMTKEDGKHHEVNVQFSWYGTTIKRDKSGAYLFLPDGNAKPYVYTTPPFVRVTHGRIYSEVTC FFDHVTHRVRLYHIQGIEGQSVEVSNIVDIRKVYNREIAMKISSDIKSQNRFYTDLNGYQIQPRMTLSKL PLQANVYPMTTMAYIQDAKHRLTLLSAQSLGVSSLNSGQIEVIMDRRLMQDDNRGLEQGIQDNKITANLF RILLEKRSAVNTEEEKKSVSYPSLLSHITSSLMNHPVIPMANKFSSPTLELQGEFSPLQSSLPCDIHLVN LRTIQSKVGNGHSNEAALILHRKGFDCRFSSKGTGLFCSTTQGKILVQKLLNKFIVESLTPSSLSLMHSP PGTQNISEINLSPMEISTFRIQLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002372 |
| ORF Size | 3432 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002372.3 |
| RefSeq Size | 5128 bp |
| RefSeq ORF | 3435 bp |
| Locus ID | 4124 |
| UniProt ID | Q16706 |
| Domains | Glyco_hydro_38 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| MW | 131 kDa |
| Gene Summary | This gene encodes a glycosyl hydrolase that localizes to the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus. [provided by RefSeq, Dec 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220186L1 | Lenti ORF clone of Human mannosidase, alpha, class 2A, member 1 (MAN2A1), Myc-DDK-tagged |
CNY 10,368.00 |
|
| RC220186L2 | Lenti ORF clone of Human mannosidase, alpha, class 2A, member 1 (MAN2A1), mGFP tagged |
CNY 13,300.00 |
|
| RC220186L3 | Lenti ORF clone of Human mannosidase, alpha, class 2A, member 1 (MAN2A1), Myc-DDK-tagged |
CNY 10,368.00 |
|
| RC220186L4 | Lenti ORF clone of Human mannosidase, alpha, class 2A, member 1 (MAN2A1), mGFP tagged |
CNY 10,368.00 |
|
| RG220186 | MAN2A1 (tGFP-tagged) - Human mannosidase, alpha, class 2A, member 1 (MAN2A1) |
CNY 9,568.00 |
|
| SC127164 | MAN2A1 (untagged)-Human mannosidase, alpha, class 2A, member 1 (MAN2A1) |
CNY 7,976.00 |
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