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Dicer (DICER1) (NM_177438) Human Tagged ORF Clone
CAT＃: RC219214
- TrueORF®
DICER1 (Myc-DDK-tagged)-Human dicer 1, ribonuclease type III (DICER1), transcript variant 1
ORF Plasmid: tGFP
"NM_177438" in other vectors (3)
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CNY 12,920.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DCR1; Dicer; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; MNG1; RMSE2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219214 representing NM_177438
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAGCCCTGCTTTGCAACCCCTCAGCATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAA TGGGTCCTTTCTTTGGACTGCCATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATA TCAGGTTGAACTGCTTGAAGCAGCTCTGGATCATAATACCATCGTCTGTTTAAACACTGGCTCAGGGAAG ACATTTATTGCAGTACTACTCACTAAAGAGCTGTCCTATCAGATCAGGGGAGACTTCAGCAGAAATGGAA AAAGGACGGTGTTCTTGGTCAACTCTGCAAACCAGGTTGCTCAACAAGTGTCAGCTGTCAGAACTCATTC AGATCTCAAGGTTGGGGAATACTCAAACCTAGAAGTAAATGCATCTTGGACAAAAGAGAGATGGAACCAA GAGTTTACTAAGCACCAGGTTCTCATTATGACTTGCTATGTCGCCTTGAATGTTTTGAAAAATGGTTACT TATCACTGTCAGACATTAACCTTTTGGTGTTTGATGAGTGTCATCTTGCAATCCTAGACCACCCCTATCG AGAAATTATGAAGCTCTGTGAAAATTGTCCATCATGTCCTCGCATTTTGGGACTAACTGCTTCCATTTTA AATGGGAAATGTGATCCAGAGGAATTGGAAGAAAAGATTCAGAAACTAGAGAAAATTCTTAAGAGTAATG CTGAAACTGCAACTGACCTGGTGGTCTTAGACAGGTATACTTCTCAGCCATGTGAGATTGTGGTGGATTG TGGACCATTTACTGACAGAAGTGGGCTTTATGAAAGACTGCTGATGGAATTAGAAGAAGCACTTAATTTT ATCAATGATTGTAATATATCTGTACATTCAAAAGAAAGAGATTCTACTTTAATTTCGAAACAGATACTAT CAGACTGTCGTGCCGTATTGGTAGTTCTGGGACCCTGGTGTGCAGATAAAGTAGCTGGAATGATGGTAAG AGAACTACAGAAATACATCAAACATGAGCAAGAGGAGCTGCACAGGAAATTTTTATTGTTTACAGACACT TTCCTAAGGAAAATACATGCACTATGTGAAGAGCACTTCTCACCTGCCTCACTTGACCTGAAATTTGTAA CTCCTAAAGTAATCAAACTGCTCGAAATCTTACGCAAATATAAACCATATGAGCGACAGCAGTTTGAAAG CGTTGAGTGGTATAATAATAGAAATCAGGATAATTATGTGTCATGGAGTGATTCTGAGGATGATGATGAG GATGAAGAAATTGAAGAAAAAGAGAAGCCAGAGACAAATTTTCCTTCTCCTTTTACCAACATTTTGTGCG GAATTATTTTTGTGGAAAGAAGATACACAGCAGTTGTCTTAAACAGATTGATAAAGGAAGCTGGCAAACA AGATCCAGAGCTGGCTTATATCAGTAGCAATTTCATAACTGGACATGGCATTGGGAAGAATCAGCCTCGC AACAAACAGATGGAAGCAGAATTCAGAAAACAGGAAGAGGTACTTAGGAAATTTCGAGCACATGAGACCA ACCTGCTTATTGCAACAAGTATTGTAGAAGAGGGTGTTGATATACCAAAATGCAACTTGGTGGTTCGTTT TGATTTGCCCACAGAATATCGATCCTATGTTCAATCTAAAGGAAGAGCAAGGGCACCCATCTCTAATTAT ATAATGTTAGCGGATACAGACAAAATAAAAAGTTTTGAAGAAGACCTTAAAACCTACAAAGCTATTGAAA AGATCTTGAGAAACAAGTGTTCCAAGTCGGTTGATACTGGTGAGACTGACATTGATCCTGTCATGGATGA TGATGACGTTTTCCCACCATATGTGTTGAGGCCTGACGATGGTGGTCCACGAGTCACAATCAACACGGCC ATTGGACACATCAATAGATACTGTGCTAGATTACCAAGTGATCCGTTTACTCATCTAGCTCCTAAATGCA GAACCCGAGAGTTGCCTGATGGTACATTTTATTCAACTCTTTATCTGCCAATTAACTCACCTCTTCGAGC CTCCATTGTTGGTCCACCAATGAGCTGTGTACGATTGGCTGAAAGAGTTGTAGCTCTCATTTGCTGTGAG AAACTGCACAAAATTGGCGAACTGGATGACCATTTGATGCCAGTTGGGAAAGAGACTGTTAAATATGAAG AGGAGCTTGATTTGCATGATGAAGAAGAGACCAGTGTTCCAGGAAGACCAGGTTCCACGAAACGAAGGCA GTGCTACCCAAAAGCAATTCCAGAGTGTTTGAGGGATAGTTATCCCAGACCTGATCAGCCCTGTTACCTG TATGTGATAGGAATGGTTTTAACTACACCTTTACCTGATGAACTCAACTTTAGAAGGCGGAAGCTCTATC CTCCTGAAGATACCACAAGATGCTTTGGAATACTGACGGCCAAACCCATACCTCAGATTCCACACTTTCC TGTGTACACACGCTCTGGAGAGGTTACCATATCCATTGAGTTGAAGAAGTCTGGTTTCATGTTGTCTCTA CAAATGCTTGAGTTGATTACAAGACTTCACCAGTATATATTCTCACATATTCTTCGGCTTGAAAAACCTG CACTAGAATTTAAACCTACAGACGCTGATTCAGCATACTGTGTTCTACCTCTTAATGTTGTTAATGACTC CAGCACTTTGGATATTGACTTTAAATTCATGGAAGATATTGAGAAGTCTGAAGCTCGCATAGGCATTCCC AGTACAAAGTATACAAAAGAAACACCCTTTGTTTTTAAATTAGAAGATTACCAAGATGCCGTTATCATTC CAAGATATCGCAATTTTGATCAGCCTCATCGATTTTATGTAGCTGATGTGTACACTGATCTTACCCCACT CAGTAAATTTCCTTCCCCTGAGTATGAAACTTTTGCAGAATATTATAAAACAAAGTACAACCTTGACCTA ACCAATCTCAACCAGCCACTGCTGGATGTGGACCACACATCTTCAAGACTTAATCTTTTGACACCTCGAC ATTTGAATCAGAAGGGGAAAGCGCTTCCTTTAAGCAGTGCTGAGAAGAGGAAAGCCAAATGGGAAAGTCT GCAGAATAAACAGATACTGGTTCCAGAACTCTGTGCTATACATCCAATTCCAGCATCACTGTGGAGAAAA GCTGTTTGTCTCCCCAGCATACTTTATCGCCTTCACTGCCTTTTGACTGCAGAGGAGCTAAGAGCCCAGA CTGCCAGCGATGCTGGCGTGGGAGTCAGATCACTTCCTGCGGATTTTAGATACCCTAACTTAGACTTCGG GTGGAAAAAATCTATTGACAGCAAATCTTTCATCTCAATTTCTAACTCCTCTTCAGCTGAAAATGATAAT TACTGTAAGCACAGCACAATTGTCCCTGAAAATGCTGCACATCAAGGTGCTAATAGAACCTCCTCTCTAG AAAATCATGACCAAATGTCTGTGAACTGCAGAACGTTGCTCAGCGAGTCCCCTGGTAAGCTCCACGTTGA AGTTTCAGCAGATCTTACAGCAATTAATGGTCTTTCTTACAATCAAAATCTCGCCAATGGCAGTTATGAT TTAGCTAACAGAGACTTTTGCCAAGGAAATCAGCTAAATTACTACAAGCAGGAAATACCCGTGCAACCAA CTACCTCATATTCCATTCAGAATTTATACAGTTACGAGAACCAGCCCCAGCCCAGCGATGAATGTACTCT CCTGAGTAATAAATACCTTGATGGAAATGCTAACAAATCTACCTCAGATGGAAGTCCTGTGATGGCCGTA ATGCCTGGTACGACAGACACTATTCAAGTGCTCAAGGGCAGGATGGATTCTGAGCAGAGCCCTTCTATTG GGTACTCCTCAAGGACTCTTGGCCCCAATCCTGGACTTATTCTTCAGGCTTTGACTCTGTCAAACGCTAG TGATGGATTTAACCTGGAGCGGCTTGAAATGCTTGGCGACTCCTTTTTAAAGCATGCCATCACCACATAT CTATTTTGCACTTACCCTGATGCGCATGAGGGCCGCCTTTCATATATGAGAAGCAAAAAGGTCAGCAACT GTAATCTGTATCGCCTTGGAAAAAAGAAGGGACTACCCAGCCGCATGGTGGTGTCAATATTTGATCCCCC TGTGAATTGGCTTCCTCCTGGTTATGTAGTAAATCAAGACAAAAGCAACACAGATAAATGGGAAAAAGAT GAAATGACAAAAGACTGCATGCTGGCGAATGGCAAACTGGATGAGGATTACGAGGAGGAGGATGAGGAGG AGGAGAGCCTGATGTGGAGGGCTCCGAAGGAAGAGGCTGACTATGAAGATGATTTCCTGGAGTATGATCA GGAACATATCAGATTTATAGATAATATGTTAATGGGGTCAGGAGCTTTTGTAAAGAAAATCTCTCTTTCT CCTTTTTCAACCACTGATTCTGCATATGAATGGAAAATGCCCAAAAAATCCTCCTTAGGTAGTATGCCAT TTTCATCAGATTTTGAGGATTTTGACTACAGCTCTTGGGATGCAATGTGCTATCTGGATCCTAGCAAAGC TGTTGAAGAAGATGACTTTGTGGTGGGGTTCTGGAATCCATCAGAAGAAAACTGTGGTGTTGACACGGGA AAGCAGTCCATTTCTTACGACTTGCACACTGAGCAGTGTATTGCTGACAAAAGCATAGCGGACTGTGTGG AAGCCCTGCTGGGCTGCTATTTAACCAGCTGTGGGGAGAGGGCTGCTCAGCTTTTCCTCTGTTCACTGGG GCTGAAGGTGCTCCCGGTAATTAAAAGGACTGATCGGGAAAAGGCCCTGTGCCCTACTCGGGAGAATTTC AACAGCCAACAAAAGAACCTTTCAGTGAGCTGTGCTGCTGCTTCTGTGGCCAGTTCACGCTCTTCTGTAT TGAAAGACTCGGAATATGGTTGTTTGAAGATTCCACCAAGATGTATGTTTGATCATCCAGATGCAGATAA AACACTGAATCACCTTATATCGGGGTTTGAAAATTTTGAAAAGAAAATCAACTACAGATTCAAGAATAAG GCTTACCTTCTCCAGGCTTTTACACATGCCTCCTACCACTACAATACTATCACTGATTGTTACCAGCGCT TAGAATTCCTGGGAGATGCGATTTTGGACTACCTCATAACCAAGCACCTTTATGAAGACCCGCGGCAGCA CTCCCCGGGGGTCCTGACAGACCTGCGGTCTGCCCTGGTCAACAACACCATCTTTGCATCGCTGGCTGTA AAGTACGACTACCACAAGTACTTCAAAGCTGTCTCTCCTGAGCTCTTCCATGTCATTGATGACTTTGTGC AGTTTCAGCTTGAGAAGAATGAAATGCAAGGAATGGATTCTGAGCTTAGGAGATCTGAGGAGGATGAAGA GAAAGAAGAGGATATTGAAGTTCCAAAGGCCATGGGGGATATTTTTGAGTCGCTTGCTGGTGCCATTTAC ATGGATAGTGGGATGTCACTGGAGACAGTCTGGCAGGTGTACTATCCCATGATGCGGCCACTAATAGAAA AGTTTTCTGCAAATGTACCCCGTTCCCCTGTGCGAGAATTGCTTGAAATGGAACCAGAAACTGCCAAATT TAGCCCGGCTGAGAGAACTTACGACGGGAAGGTCAGAGTCACTGTGGAAGTAGTAGGAAAGGGGAAATTT AAAGGTGTTGGTCGAAGTTACAGGATTGCCAAATCTGCAGCAGCAAGAAGAGCCCTCCGAAGCCTCAAAG CTAATCAACCTCAGGTTCCCAATAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219214 representing NM_177438
Red=Cloning site Green=Tags(s) MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGK TFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQ EFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASIL NGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNF INDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDT FLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDE DEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPR NKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNY IMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTA IGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCE KLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYL YVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSL QMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIP STKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDL TNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRK AVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDN YCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYD LANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAV MPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTY LFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKD EMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLS PFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTG KQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENF NSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNK AYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAV KYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIY MDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKF KGVGRSYRIAKSAAARRALRSLKANQPQVPNS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177438 |
| ORF Size | 5766 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177438.3 |
| RefSeq Size | 10276 bp |
| RefSeq ORF | 5769 bp |
| Locus ID | 23405 |
| UniProt ID | Q9UPY3 |
| Protein Families | Druggable Genome |
| MW | 218.5 kDa |
| Gene Summary | This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219214L1 | Lenti ORF clone of Human dicer 1, ribonuclease type III (DICER1), transcript variant 1, Myc-DDK-tagged |
CNY 16,060.00 |
|
| RG219214 | DICER1 (tGFP-tagged) - Human dicer 1, ribonuclease type III (DICER1), transcript variant 1 |
CNY 15,580.00 |
|
| SC309637 | DICER1 (untagged)-Human dicer 1, ribonuclease type III (DICER1), transcript variant 1 |
CNY 14,216.00 |
