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ATP dependent metalloprotease YME1L1 (YME1L1) (NM_139312) Human Tagged ORF Clone
CAT＃: RC218760
- TrueORF®
YME1L1 (Myc-DDK-tagged)-Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid: tGFP
"NM_139312" in other vectors (4)
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CNY 8,170.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FTSH; MEG4; OPA11; PAMP; YME1L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218760 representing NM_139312
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTCCTTGTCGAGCACGGTGCAACCCCAGGTTACAGTTCCTCTGAGTCATCTCATCAATGCCTTCC ATACACCAAAAAACACTTCTGTTTCTCTCAGTGGAGTGTCAGTTTCTCAAAACCAGCATCGAGATGTAGT TCCTGAGCATGAGGCTCCCAGCAGTGAGTGTATGTTCAGTGACTTCCTGACGAAGCTTAACATTGTTTCA ATTGGCAAAGGAAAAATATTCGAAGGGTACAGATCCATGTTCATGGAGCCAGCAAAAAGGATGAAGAAGA GCTTGGACACAACCGATAACTGGCACATCCGTCCAGAACCCTTCTCCCTCTCAATCCCTCCTTCACTTAA CTTAAGGGACCTTGGATTATCTGAACTAAAAATTGGACAGATTGATCAGCTGGTAGAAAATCTACTTCCT GGATTTTGTAAAGGCAAAAACATTTCTTCCCATTGGCATACATCCCATGTCTCTGCACAATCCTTCTTTG AAAATAAATATGGTAACTTAGATATATTTAGTACATTACGTTCCTCTTGCTTGTATCGACATCATTCAAG AGCTCTTCAAAGCATTTGTTCAGATCTTCAGTACTGGCCAGTTTTCATACAGTCTCGGGGTTTTAAAACT TTGAAATCAAGGACACGACGTCTCCAGTCTACCTCCGAGAGATTAGCTGAAACACAGAATATAGCGCCAT CATTCGTGAAGGGGTTTCTTTTGCGGGACAGAGGATCAGATGTTGAGAGTTTGGACAAACTCATGAAAAC CAAAAATATACCTGAAGCTCACCAAGATGCATTTAAAACTGGTTTTGCGGAAGGTTTTCTGAAAGCTCAA GCACTCACACAAAAAACCAATGATTCCCTAAGGCGAACCCGTCTGATTCTCTTCGTTCTGCTGCTATTCG GCATTTATGGACTTCTAAAAAACCCATTTTTATCTGTCCGCTTCCGGACAACAACAGGGCTTGATTCTGC AGTAGATCCTGTCCAGATGAAAAATGTCACCTTTGAACATGTTAAAGGGGTGGAGGAAGCTAAACAAGAA TTACAGGAAGTTGTTGAATTCTTGAAAAATCCACAAAAATTTACTATTCTTGGAGGTAAACTTCCAAAAG GAATTCTTTTAGTTGGACCCCCAGGGACTGGAAAGACACTTCTTGCCCGAGCTGTGGCGGGAGAAGCTGA TGTTCCTTTTTATTATGCTTCTGGATCCGAATTTGATGAGATGTTTGTGGGTGTGGGAGCCAGCCGTATC AGAAATCTTTTTAGGGAAGCAAAGGCGAATGCTCCTTGTGTTATATTTATTGATGAATTAGATTCTGTTG GTGGGAAGAGAATTGAATCTCCAATGCATCCATATTCAAGGCAGACCATAAATCAACTTCTTGCTGAAAT GGATGGTTTTAAACCCAATGAAGGAGTTATCATAATAGGAGCCACAAACTTCCCAGAGGCATTAGATAAT GCCTTAATACGTCCTGGTCGTTTTGACATGCAAGTTACAGTTCCAAGGCCAGATGTAAAAGGTCGAACAG AAATTTTGAAATGGTATCTCAATAAAATAAAGTTTGATCAATCCGTTGATCCAGAAATTATAGCTCGAGG TACTGTTGGCTTTTCCGGAGCAGAGTTGGAGAATCTTGTGAACCAGGCTGCATTAAAAGCAGCTGTTGAT GGAAAAGAAATGGTTACCATGAAGGAGCTGGAGTTTTCCAAAGACAAAATTCTAATGGGGCCTGAAAGAA GAAGTGTGGAAATTGATAACAAAAACAAAACCATCACAGCATATCATGAATCTGGTCATGCCATTATTGC ATATTACACAAAAGATGCAATGCCTATCAACAAAGCTACAATCATGCCACGGGGGCCAACACTTGGACAT GTGTCCCTGTTACCTGAGAATGACAGATGGAATGAAACTAGAGCCCAGCTGCTTGCACAAATGGATGTTA GTATGGGAGGAAGAGTGGCAGAGGAGCTTATATTTGGAACCGACCATATTACAACAGGTGCTTCCAGTGA TTTTGATAATGCCACTAAAATAGCAAAGCGGATGGTTACCAAATTTGGAATGAGTGAAAAGCTTGGAGTT ATGACCTACAGTGATACAGGGAAACTAAGTCCAGAAACCCAATCTGCCATCGAACAAGAAATAAGAATCC TTCTAAGGGACTCATATGAACGAGCAAAACATATCTTGAAAACTCATGCAAAGGAGCATAAGAATCTCGC AGAAGCTTTATTGACCTATGAGACTTTGGATGCCAAAGAGATTCAAATTGTTCTTGAGGGGAAAAAGTTG GAAGTGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218760 representing NM_139312
Red=Cloning site Green=Tags(s) MFSLSSTVQPQVTVPLSHLINAFHTPKNTSVSLSGVSVSQNQHRDVVPEHEAPSSECMFSDFLTKLNIVS IGKGKIFEGYRSMFMEPAKRMKKSLDTTDNWHIRPEPFSLSIPPSLNLRDLGLSELKIGQIDQLVENLLP GFCKGKNISSHWHTSHVSAQSFFENKYGNLDIFSTLRSSCLYRHHSRALQSICSDLQYWPVFIQSRGFKT LKSRTRRLQSTSERLAETQNIAPSFVKGFLLRDRGSDVESLDKLMKTKNIPEAHQDAFKTGFAEGFLKAQ ALTQKTNDSLRRTRLILFVLLLFGIYGLLKNPFLSVRFRTTTGLDSAVDPVQMKNVTFEHVKGVEEAKQE LQEVVEFLKNPQKFTILGGKLPKGILLVGPPGTGKTLLARAVAGEADVPFYYASGSEFDEMFVGVGASRI RNLFREAKANAPCVIFIDELDSVGGKRIESPMHPYSRQTINQLLAEMDGFKPNEGVIIIGATNFPEALDN ALIRPGRFDMQVTVPRPDVKGRTEILKWYLNKIKFDQSVDPEIIARGTVGFSGAELENLVNQAALKAAVD GKEMVTMKELEFSKDKILMGPERRSVEIDNKNKTITAYHESGHAIIAYYTKDAMPINKATIMPRGPTLGH VSLLPENDRWNETRAQLLAQMDVSMGGRVAEELIFGTDHITTGASSDFDNATKIAKRMVTKFGMSEKLGV MTYSDTGKLSPETQSAIEQEIRILLRDSYERAKHILKTHAKEHKNLAEALLTYETLDAKEIQIVLEGKKL EVR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_139312 |
| ORF Size | 2319 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_139312.3 |
| RefSeq Size | 4036 bp |
| RefSeq ORF | 2322 bp |
| Locus ID | 10730 |
| UniProt ID | Q96TA2 |
| Domains | Peptidase_M41, AAA |
| Protein Families | Druggable Genome, Protease |
| MW | 86.3 kDa |
| Gene Summary | The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218760L3 | Lenti-ORF clone of YME1L1 (Myc-DDK-tagged)-Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 10,070.00 |
|
| RC218760L4 | Lenti-ORF clone of YME1L1 (mGFP-tagged)-Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 10,070.00 |
|
| RG218760 | YME1L1 (tGFP-tagged) - Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 7,832.00 |
|
| SC120718 | YME1L1 (untagged)-Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,672.00 |
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