HNF 4 alpha (HNF4A) (NM_000457) Human Tagged ORF Clone
CAT#: RC217863
HNF4A (Myc-DDK-tagged)-Human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2
ORF Plasmid: tGFP
"NM_000457" in other vectors (6)
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CNY 5,488.00
Cited in 7 publications. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF-14; TCF14 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC217863 representing NM_000457
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGACTCTCCAAAACCCTCGTCGACATGGACATGGCCGACTACAGTGCTGCACTGGACCCAGCCTACA CCACCCTGGAATTTGAGAATGTGCAGGTGTTGACGATGGGCAATGACACGTCCCCATCAGAAGGCACCAA CCTCAACGCGCCCAACAGCCTGGGTGTCAGCGCCCTGTGTGCCATCTGCGGGGACCGGGCCACGGGCAAA CACTACGGTGCCTCGAGCTGTGACGGCTGCAAGGGCTTCTTCCGGAGGAGCGTGCGGAAGAACCACATGT ACTCCTGCAGATTTAGCCGGCAGTGCGTGGTGGACAAAGACAAGAGGAACCAGTGCCGCTACTGCAGGCT CAAGAAATGCTTCCGGGCTGGCATGAAGAAGGAAGCCGTCCAGAATGAGCGGGACCGGATCAGCACTCGA AGGTCAAGCTATGAGGACAGCAGCCTGCCCTCCATCAATGCGCTCCTGCAGGCGGAGGTCCTGTCCCGAC AGATCACCTCCCCCGTCTCCGGGATCAACGGCGACATTCGGGCGAAGAAGATTGCCAGCATCGCAGATGT GTGTGAGTCCATGAAGGAGCAGCTGCTGGTTCTCGTTGAGTGGGCCAAGTACATCCCAGCTTTCTGCGAG CTCCCCCTGGACGACCAGGTGGCCCTGCTCAGAGCCCATGCTGGCGAGCACCTGCTGCTCGGAGCCACCA AGAGATCCATGGTGTTCAAGGACGTGCTGCTCCTAGGCAATGACTACATTGTCCCTCGGCACTGCCCGGA GCTGGCGGAGATGAGCCGGGTGTCCATACGCATCCTTGACGAGCTGGTGCTGCCCTTCCAGGAGCTGCAG ATCGATGACAATGAGTATGCCTACCTCAAAGCCATCATCTTCTTTGACCCAGATGCCAAGGGGCTGAGCG ATCCAGGGAAGATCAAGCGGCTGCGTTCCCAGGTGCAGGTGAGCTTGGAGGACTACATCAACGACCGCCA GTATGACTCGCGTGGCCGCTTTGGAGAGCTGCTGCTGCTGCTGCCCACCTTGCAGAGCATCACCTGGCAG ATGATCGAGCAGATCCAGTTCATCAAGCTCTTCGGCATGGCCAAGATTGACAACCTGTTGCAGGAGATGC TGCTGGGAGGGTCCCCCAGCGATGCACCCCATGCCCACCACCCCCTGCACCCTCACCTGATGCAGGAACA TATGGGAACCAACGTCATCGTTGCCAACACAATGCCCACTCACCTCAGCAACGGACAGATGTGTGAGTGG CCCCGACCCAGGGGACAGGCAGCCACCCCTGAGACCCCACAGCCCTCACCGCCAGGTGGCTCAGGGTCTG AGCCCTATAAGCTCCTGCCGGGAGCCGTCGCCACAATCGTCAAGCCCCTCTCTGCCATCCCCCAGCCGAC CATCACCAAGCAGGAAGTTATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217863 representing NM_000457
Red=Cloning site Green=Tags(s) MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGK HYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTR RSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCE LPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQ IDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQ MIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEW PRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000457 |
ORF Size | 1422 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000457.5 |
RefSeq Size | 4737 bp |
RefSeq ORF | 1425 bp |
Locus ID | 3172 |
UniProt ID | P41235 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Nuclear Hormone Receptor, Transcription Factors |
Protein Pathways | Maturity onset diabetes of the young |
MW | 53.2 kDa |
Gene Summary | The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012] |
Citations (7)
The use of this cDNA Clones has been cited in the following citations: |
---|
HNF4A defines tissue-specific circadian rhythms by beaconing BMAL1::CLOCK chromatin binding and shaping the rhythmic chromatin landscape
,null,
Nature Communications
,PubMed ID 34732735
[HNF 4 alpha]
|
Molecular Basis for Autosomal-Dominant Renal Fanconi Syndrome Caused by HNF4A
,Marchesin, V;Pérez-Martí, A;Le Meur, G;Pichler, R;Grand, K;Klootwijk, ED;Kesselheim, A;Kleta, R;Lienkamp, S;Simons, M;,
Cell Rep
,PubMed ID 31875549
[HNF 4 alpha]
|
A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts
,Xie, B;Sun, D;Du, Y;Jia, J;Sun, S;Xu, J;Liu, Y;Xiang, C;Chen, S;Xie, H;Wang, Q;Li, G;Lyu, X;Shen, H;Li, S;Wu, M;Zhang, X;Pu, Y;Xiang, K;Lai, W;Du, P;Yuan, Z;Li, C;Shi, Y;Lu, S;Deng, H;,
Cell Res.
,PubMed ID 31270412
[HNF 4 alpha]
|
Ferroptosis is governed by differential regulation of transcription in liver cancer
,Zhang, X;Du, L;Qiao, Y;Zhang, X;Zheng, W;Wu, Q;Chen, Y;Zhu, G;Liu, Y;Bian, Z;Guo, S;Yang, Y;Ma, L;Yu, Y;Pan, Q;Sun, F;Wang, J;,
Redox Biol
,PubMed ID 31108460
[HNF 4 alpha]
|
The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury
,Chae, YJ;Jun, DW;Lee, JS;Saeed, WK;Kang, HT;Jang, K;Lee, JH;,
Gut Liver
,PubMed ID 30602218
[HNF 4 alpha]
|
circRNA_104075 stimulates YAP-dependent tumorigenesis through the regulation of HNF4a and may serve as a diagnostic marker in hepatocellular carcinoma
,Zhang, X;Xu, Y;Qian, Z;Zheng, W;Wu, Q;Chen, Y;Zhu, G;Liu, Y;Bian, Z;Xu, W;Zhang, Y;Sun, F;Pan, Q;Wang, J;Du, L;Yu, Y;,
Cell Death Dis
,PubMed ID 30361504
[HNF 4 alpha]
|
Regulatory crosstalk between lineage-survival oncogenes KLF5, GATA4 and GATA6 cooperatively promotes gastric cancer development
,Chia, NY;Deng, N;Das, K;Huang, D;Hu, L;Zhu, Y;Lim, KH;Lee, MH;Wu, J;Sam, XX;Tan, GS;Wan, WK;Yu, W;Gan, A;Tan, AL;Tay, ST;Soo, KC;Wong, WK;Dominguez, LT;Ng, HH;Rozen, S;Goh, LK;Teh, BT;Tan, P;,
Gut July 2014
,PubMed ID 25053715
[HNF 4 alpha]
|
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217863L1 | Lenti ORF clone of Human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2, Myc-DDK-tagged |
CNY 7,888.00 |
|
RC217863L2 | Lenti ORF clone of Human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2, mGFP tagged |
CNY 7,888.00 |
|
RC217863L3 | Lenti ORF clone of Human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2, Myc-DDK-tagged |
CNY 7,888.00 |
|
RC217863L4 | Lenti ORF clone of Human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2, mGFP tagged |
CNY 7,888.00 |
|
RG217863 | HNF4A (tGFP-tagged) - Human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2 |
CNY 7,088.00 |
|
SC123863 | HNF4A (untagged)-Human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2 |
CNY 5,488.00 |
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