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GRSF1 (NM_001098477) Human Tagged ORF Clone
CAT＃: RC216987
GRSF1 (Myc-DDK-tagged)-Human G-rich RNA sequence binding factor 1 (GRSF1), transcript variant 2
ORF Plasmid: tGFP
"NM_001098477" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216987 representing NM_001098477
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGATGTGCTTAACTTTTTTTCAGACTGCAGAATCCGCAACGGTGAGAATGGAATACATTTTCTCC TAAACAGAGATGGGAAACGAAGGGGTGATGCCTTAATTGAAATGGAGTCAGAGCAGGATGTGCAGAAAGC CTTAGAGAAGCACCGCATGTACATGGGCCAGCGGTATGTGGAAGTATATGAGATAAACAATGAAGATGTG GATGCCTTAATGAAGAGCTTGCAGGTCAAATCTTCGCCTGTGGTAAATGATGGTGTGGTTCGTTTGAGAG GACTTCCTTATAGTTGCAATGAGAAAGACATTGTAGACTTCTTTGCAGGACTGAATATAGTTGACATTAC TTTTGTGATGGACTATAGAGGGAGGCGAAAAACAGGGGAAGCCTATGTGCAATTTGAAGAACCAGAAATG GCCAACCAAGCCCTGTTGAAACACAGGGAAGAAATTGGTAATCGATACATCGAGATATTTCCAAGCAGAA GGAATGAAGTTCGAACACATGTCGGTTCTTATAAGGGAAAGAAAATCGCATCTTTTCCTACTGCTAAGTA TATAACTGAGCCAGAAATGGTCTTTGAAGAACATGAAGTAAATGAGGATATTCAACCCATGACAGCTTTT GAAAGTGAGAAGGAAATAGAATTGCCTAAGGAGGTGCCAGAAAAGCTTCCAGAGGCTGCTGATTTTGGAA CTACGTCTTCTCTGCATTTTGTCCACATGAGAGGATTACCTTTCCAAGCCAATGCCCAAGACATTATAAA CTTTTTTGCTCCACTCAAGCCTGTTAGAATCACCATGGAATACAGCTCCAGTGGGAAGGCCACTGGAGAA GCTGATGTGCACTTTGAGACCCATGAGGATGCTGTTGCAGCGATGCTCAAGGATCGGTCCCACGTTCATC ATAGGTATATTGAACTGTTCCTGAATTCATGTCCAAAAGGAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216987 representing NM_001098477
Red=Cloning site Green=Tags(s) MEDVLNFFSDCRIRNGENGIHFLLNRDGKRRGDALIEMESEQDVQKALEKHRMYMGQRYVEVYEINNEDV DALMKSLQVKSSPVVNDGVVRLRGLPYSCNEKDIVDFFAGLNIVDITFVMDYRGRRKTGEAYVQFEEPEM ANQALLKHREEIGNRYIEIFPSRRNEVRTHVGSYKGKKIASFPTAKYITEPEMVFEEHEVNEDIQPMTAF ESEKEIELPKEVPEKLPEAADFGTTSSLHFVHMRGLPFQANAQDIINFFAPLKPVRITMEYSSSGKATGE ADVHFETHEDAVAAMLKDRSHVHHRYIELFLNSCPKGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001098477 |
| ORF Size | 954 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001098477.2 |
| RefSeq Size | 6482 bp |
| RefSeq ORF | 957 bp |
| Locus ID | 2926 |
| UniProt ID | Q12849 |
| MW | 36.4 kDa |
| Gene Summary | The protein encoded by this gene is a cellular protein that binds RNAs containing the G-rich element. The protein is localized in the cytoplasm, and has been shown to stimulate translation of viral mRNAs in vitro. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216987L3 | Lenti ORF clone of Human G-rich RNA sequence binding factor 1 (GRSF1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216987L4 | Lenti ORF clone of Human G-rich RNA sequence binding factor 1 (GRSF1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG216987 | GRSF1 (tGFP-tagged) - Human G-rich RNA sequence binding factor 1 (GRSF1), transcript variant 2 |
CNY 4,370.00 |
|
| SC316360 | GRSF1 (untagged)-Human G-rich RNA sequence binding factor 1 (GRSF1), transcript variant 2 |
CNY 6,270.00 |
