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AASDH (NM_181806) Human Tagged ORF Clone
CAT＃: RC216923
AASDH (Myc-DDK-tagged)-Human aminoadipate-semialdehyde dehydrogenase (AASDH)
ORF Plasmid: tGFP
"NM_181806" in other vectors (4)
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CNY 7,648.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACSF4; LYS2; NRPS998; NRPS1098 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216923 representing NM_181806
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCTTCAGGAATTGGTGCATAAGGCTGCCTCCTGTTATATGGACAGAGTAGCTGTATGTTTTGATG AATGCAACAACCAGCTTCCAGTTTACTACACCTACAAGACTGTGGTTAATGCTGCTTCTGAATTATCAAA TTTTCTGCTGTTACACTGTGACTTTCAAGGAATTCGGGAAATTGGTCTCTACTGCCAACCTGGGATAGAC TTACCCTCTTGGATTTTAGGAATTCTCCAAGTCCCGGCTGCTTATGTACCTATCGAGCCAGATTCACCAC CGTCATTATCAACTCATTTTATGAAAAAATGTAATCTAAAGTATATCCTTGTTGAAAAAAAACAAATTAA TAAATTTAAATCTTTTCATGAAACATTATTGAACTATGATACATTTACAGTGGAACATAATGACCTAGTG CTCTTCAGACTTCACTGGAAAAATACTGAGGTGAACTTGATGCTAAATGATGGAAAAGAGAAATATGAAA AAGAAAAAATAAAAAGCATAAGTTCTGAGCATGTCAATGAAGAAAAAGCAGAAGAACACATGGATCTGAG GCTAAAGCATTGCTTAGCCTATGTTCTACATACATCAGGGACTACAGGGATACCGAAGATTGTCAGAGTG CCTCATAAGTGTATAGTACCAAATATCCAGCATTTTCGGGTACTTTTTGACATCACACAAGAAGATGTTT TGTTTCTGGCTTCACCTCTGACCTTCGATCCTTCTGTTGTGGAAATATTTCTTGCTCTATCAAGTGGTGC CTCTCTGCTTATTGTACCAACTTCCGTCAAGTTGCTCCCATCAAAATTAGCCAGCGTTCTCTTTTCCCAT CATAGAGTGACTGTTTTGCAGGCAACACCAACATTGCTTAGAAGATTTGGATCTCAGCTTATCAAGTCAA CTGTTTTGTCAGCCACTACTTCTCTTCGAGTATTAGCCCTTGGTGGTGAAGCGTTTCCATCATTGACAGT TCTCAGAAGCTGGAGAGGAGAAGGCAATAAAACACAAATATTTAATGTTTATGGTATCACAGAGGTATCA AGTTGGGCGACCATTTATAGGATTCCAGAGAAGACTCTTAACTCTACTCTCAAATGTGAATTGCCTGTAC AACTGGGATTTCCACTTCTTGGAACAGTAGTTGAAGTCAGAGATACTAATGGCTTCACAATTCAGGAAGG CAGTGGCCAAGTATTTTTAGGTGGCAGAAACAGAGTGTGTTTTCTTGATGATGAAGTGACAGTACCACTT GGCACAATGCGAGCTACAGGAGACTTTGTGACTGTGAAAGATGGAGAGATTTTTTTTTTGGGACGAAAAG ACAGTCAGATCAAACGTCATGGCAAACGTCTTAACATTGAACTTGTGCAACAGGTTGCTGAAGAGCTTCA GCAAGTGGAGTCTTGTGCAGTTACATGGTATAATCAGGAAAAATTAATTCTCTTCATGGTGTCTAAAGAT GCTTCAGTAAAAGAATACATCTTTAAAGAACTGCAGAAATATCTTCCAAGTCATGCAGTCCCGGATGAGC TTGTATTGATCGACTCTCTACCATTTACATCCCACGGCAAAATTGATGTTTCTGAGTTAAACAAGATATA TTTAAACTACATAAACTTGAAGTCTGAGAATAAGCTCAGTGGGAAAGAGGACCTTTGGGAAAAATTACAG TATTTGTGGAAGTCTACTCTGAATCTCCCAGAAGATCTTTTGAGGGTTCCTGATGAGTCACTCTTCTTAA ATAGTGGTGGAGATTCCTTAAAGTCCATCCGGCTCCTCAGTGAGATTGAAAAACTTGTTGGTACATCAGT ACCTGGGCTTCTGGAAATTATTCTCAGCAGTTCCATTTTAGAGATTTATAATCACATCCTTCAAACAGTG GTTCCAGATGAAGATGTGACATTCAGGAAGAGTTGTGCCACAAAAAGGAAACTCAGCGACATTAATCAAG AGGAAGCCAGTGGAACATCTTTACATCAGAAAGCCATCATGACTTTCACTTGCCACAATGAGATTAATGC TTTTGTTGTACTGAGCAGAGGGAGTCAAATTTTGTCTCTGAATTCCACTAGGTTTTTAACAAAGTTAGGA CATTGCTCTTCAGCCTGTCCTTCTGACTCAGTTTCACAGACCAACATTCAAAATTTGAAAGGCTTAAATT CTCCAGTTCTTATTGGGAAGTCAAAAGATCCATCCTGTGTTGCAAAAGTTTCTGAAGAGGGGAAACCTGC GATAGGGACTCAGAAAATGGAGTTACATGTGAGGTGGAGGTCAGACACAGGCAAATGTGTAGATGCTTCA CCGCTGGTTGTAATACCCACTTTTGATAAGTCATCTACAACTGTGTACATTGGTTCCCATTCTCATAGAA TGAAGGCAGTTGACTTTTACTCTGGGAAGGTAAAATGGGAACAGATTTTGGGAGATCGAATTGAATCCTC AGCATGTGTATCTAAGTGTGGAAACTTTATTGTGGTGGGCTGTTATAATGGATTAGTTTATGTTCTGAAA AGTAATAGTGGAGAAAAATACTGGATGTTTACTACTGAAGATGCTGTCAAAAGCTCGGCAACCATGGATC CAACCACAGGACTCATTTACATTGGATCTCATGACCAGCACGCATATGCTTTAGATATTTATAGAAAGAA GTGTGTTTGGAAGTCAAAATGTGGAGGAACTGTCTTTTCCTCTCCGTGTTTGAACCTGATTCCACATCAT TTGTATTTTGCTACATTGGGAGGACTTTTACTGGCTGTAAATCCTGCTACTGGGAACGTTATTTGGAAAC ATTCCTGTGGAAAACCACTCTTCTCTTCCCCACAATGTTGCTCACAGTATATTTGTATTGGCTGTGTAGA TGGGAATTTACTCTGCTTTACTCACTTTGGAGAACAGGTTTGGCAGTTCTCTACCAGTGGACCAATCTTT TCATCCCCGTGTACCTCACCATCAGAGCAAAAAATATTTTTTGGTTCCCATGATTGCTTTATCTACTGTT GTAACATGAAAGGTCACCTGCAGTGGAAATTTGAAACTACTTCAAGGGTCTATGCAACACCGTTTGCTTT CCATAACTACAATGGCAGCAATGAAATGTTGCTGGCAGCAGCATCTACTGATGGGAAAGTGTGGATCTTG GAATCTCAGAGTGGACAATTGCAAAGTGTTTATGAACTTCCTGGAGAAGTCTTCTCTTCTCCTGTGGTCC TGGAATCAATGCTCATTATTGGGTGTAGAGATAATTATGTTTATTGTCTGGATTTATTGGGTGGCAATCA AAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216923 representing NM_181806
Red=Cloning site Green=Tags(s) MTLQELVHKAASCYMDRVAVCFDECNNQLPVYYTYKTVVNAASELSNFLLLHCDFQGIREIGLYCQPGID LPSWILGILQVPAAYVPIEPDSPPSLSTHFMKKCNLKYILVEKKQINKFKSFHETLLNYDTFTVEHNDLV LFRLHWKNTEVNLMLNDGKEKYEKEKIKSISSEHVNEEKAEEHMDLRLKHCLAYVLHTSGTTGIPKIVRV PHKCIVPNIQHFRVLFDITQEDVLFLASPLTFDPSVVEIFLALSSGASLLIVPTSVKLLPSKLASVLFSH HRVTVLQATPTLLRRFGSQLIKSTVLSATTSLRVLALGGEAFPSLTVLRSWRGEGNKTQIFNVYGITEVS SWATIYRIPEKTLNSTLKCELPVQLGFPLLGTVVEVRDTNGFTIQEGSGQVFLGGRNRVCFLDDEVTVPL GTMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRLNIELVQQVAEELQQVESCAVTWYNQEKLILFMVSKD ASVKEYIFKELQKYLPSHAVPDELVLIDSLPFTSHGKIDVSELNKIYLNYINLKSENKLSGKEDLWEKLQ YLWKSTLNLPEDLLRVPDESLFLNSGGDSLKSIRLLSEIEKLVGTSVPGLLEIILSSSILEIYNHILQTV VPDEDVTFRKSCATKRKLSDINQEEASGTSLHQKAIMTFTCHNEINAFVVLSRGSQILSLNSTRFLTKLG HCSSACPSDSVSQTNIQNLKGLNSPVLIGKSKDPSCVAKVSEEGKPAIGTQKMELHVRWRSDTGKCVDAS PLVVIPTFDKSSTTVYIGSHSHRMKAVDFYSGKVKWEQILGDRIESSACVSKCGNFIVVGCYNGLVYVLK SNSGEKYWMFTTEDAVKSSATMDPTTGLIYIGSHDQHAYALDIYRKKCVWKSKCGGTVFSSPCLNLIPHH LYFATLGGLLLAVNPATGNVIWKHSCGKPLFSSPQCCSQYICIGCVDGNLLCFTHFGEQVWQFSTSGPIF SSPCTSPSEQKIFFGSHDCFIYCCNMKGHLQWKFETTSRVYATPFAFHNYNGSNEMLLAAASTDGKVWIL ESQSGQLQSVYELPGEVFSSPVVLESMLIIGCRDNYVYCLDLLGGNQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181806 |
| ORF Size | 3294 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181806.4 |
| RefSeq Size | 3573 bp |
| RefSeq ORF | 3297 bp |
| Locus ID | 132949 |
| UniProt ID | Q4L235 |
| Protein Pathways | Lysine biosynthesis, Lysine degradation, Metabolic pathways |
| MW | 122.4 kDa |
| Gene Summary | This gene encodes a member of the non-ribosome peptide syntesase (NRPS) enzyme family. The encoded protein contains an AMP-binding domain, PP-binding (phosphopantetheine, or pantetheine 4'phosphate-binding) domain and the Pyrrolo-quinoline quinon (PQQ) binding domain. The protein is expressed in several adult tissues. [provided by RefSeq, Apr 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216923L3 | Lenti ORF clone of Human aminoadipate-semialdehyde dehydrogenase (AASDH), Myc-DDK-tagged |
CNY 12,830.00 |
|
| RC216923L4 | Lenti ORF clone of Human aminoadipate-semialdehyde dehydrogenase (AASDH), mGFP tagged |
CNY 12,830.00 |
|
| RG216923 | AASDH (tGFP-tagged) - Human aminoadipate-semialdehyde dehydrogenase (AASDH) |
CNY 11,970.00 |
|
| SC309316 | AASDH (untagged)-Human aminoadipate-semialdehyde dehydrogenase (AASDH) |
CNY 7,656.00 |
