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THRAP3 (NM_005119) Human Tagged ORF Clone
CAT＃: RC213342
THRAP3 (Myc-DDK-tagged)-Human thyroid hormone receptor associated protein 3 (THRAP3)
ORF Plasmid: tGFP
"NM_005119" in other vectors (6)
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CNY 6,992.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BCLAF2; TRAP150 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213342 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAAAAACAAACAAATCCAAGTCTGGATCTCGCTCTTCTCGCTCAAGATCTGCATCAAGATCTCGTT CTCGTTCATTTTCGAAGTCTCGGTCCCGAAGCCGATCTCTCTCTCGTTCAAGGAAGCGCAGGCTGAGTTC TAGGTCTCGTTCCAGATCATATTCTCCAGCTCATAACAGAGAAAGAAACCACCCAAGAGTATATCAGAAT CGGGATTTCCGAGGTCACAACAGAGGCTATAGAAGGCCCTATTATTTCCGTGGGCGTAACAGAGGCTTTT ATCCATGGGGCCAATATAACCGAGGAGGCTATGGAAACTACCGCTCAAATTGGCAGAATTACCGGCAAGC ATACAGTCCTCGTCGAGGCCGTTCAAGATCCCGGTCCCCAAAGAGAAGGTCCCCTTCACCAAGGTCCAGG AGCCATTCTAGAAACTCTGATAAGTCGTCTTCTGACCGGTCAAGGCGCTCCTCATCCTCCCGTTCTTCCT CCAACCATAGCCGAGTTGAATCTTCTAAGCGCAAGTCTGCAAAGGAGAAAAAGTCCTCTTCTAAGGATAG CCGGCCATCTCAGGCTGCCGGGGATAACCAGGGAGATGAGGCCAAGGAGCAGACATTCTCTGGAGGCACC TCTCAAGATACAAAAGCATCTGAGAGCTCGAAGCCATGGCCAGATGCCACCTACGGCACTGGTTCTGCAT CACGGGCCTCAGCAGTTTCTGAGCTGAGTCCTCGGGAGCGAAGCCCAGCTCTCAAAAGCCCCCTCCAGTC TGTGGTGGTGAGGCGGCGGTCACCCCGTCCTAGCCCCGTGCCAAAACCTAGTCCTCCACTTTCCAGCACA TCCCAGATGGGCTCAACTCTGCCGAGTGGTGCCGGGTATCAGTCTGGGACACACCAAGGTCAGTTCGACC ATGGTTCTGGGTCCCTGAGTCCATCCAAAAAGAGCCCTGTGGGTAAGAGTCCACCATCCACTGGCTCCAC ATATGGCTCATCTCAGAAGGAGGAGAGTGCTGCTTCAGGAGGAGCAGCCTATACAAAGAGGTATCTAGAA GAGCAGAAGACAGAGAATGGAAAAGATAAGGAACAGAAACAAACAAATACCGATAAAGAAAAAATAAAAG AGAAAGGGAGCTTCTCTGACACAGGCTTGGGTGATGGAAAAATGAAATCTGATTCTTTTGCTCCCAAAAC TGATTCTGAGAAGCCTTTTCGGGGCAGTCAGTCTCCCAAAAGGTATAAGCTCCGAGATGACTTTGAGAAG AAGATGGCTGACTTCCACAAGGAGGAGATGGATGATCAAGATAAGGACAAAGCTAAGGGAAGAAAGGAAT CTGAGTTTGATGATGAACCCAAATTTATGTCTAAAGTCATAGGTGCAAACAAAAACCAGGAGGAGGAGAA GTCAGGCAAATGGGAGGGCCTGGTATATGCACCTCCAGGGAAGGAAAAGCAGAGAAAAACAGAGGAGCTG GAGGAGGAGTCTTTCCCAGAGAGATCCAAAAAGGAAGATCGGGGCAAGAGAAGCGAAGGTGGGCACAGGG GCTTTGTGCCTGAGAAGAATTTCCGAGTGACTGCTTATAAAGCAGTCCAGGAGAAAAGCTCATCACCTCC CCCAAGAAAGACCTCTGAGAGCCGAGACAAGCTGGGAGCGAAAGGAGATTTTCCCACAGGAAAGTCTTCC TTTTCCATTACTCGAGAGGCACAGGTCAATGTCCGGATGGACTCTTTTGATGAGGACCTCGCACGACCCA GTGGCTTATTGGCTCAGGAACGCAAGCTTTGCCGAGATCTAGTCCATAGCAACAAAAAGGAACAGGAGTT TCGTTCCATTTTCCAGCACATACAATCAGCTCAGTCTCAGCGTAGCCCCTCAGAACTGTTTGCCCAACAT ATAGTGACCATTGTTCACCATGTTAAAGAGCATCACTTTGGGTCCTCAGGAATGACATTACATGAACGCT TTACTAAATACCTAAAGAGAGGAACTGAGCAGGAGGCAGCCAAAAACAAGAAAAGCCCAGAGATACACAG GAGAATAGACATTTCCCCCAGTACATTCAGAAAACATGGTTTGGCTCATGATGAAATGAAAAGTCCCCGG GAACCTGGCTACAAGGCTGAGGGAAAATACAAAGATGATCCTGTTGATCTCCGCCTTGATATTGAACGTC GTAAAAAACATAAGGAGAGAGATCTTAAACGAGGTAAATCGAGAGAATCAGTGGATTCCCGAGACTCCAG TCACTCAAGGGAAAGGTCAGCTGAAAAAACAGAGAAAACTCATAAAGGATCAAAGAAACAGAAGAAGCAT CGGAGAGCAAGAGACAGGTCCAGATCCTCCTCCTCTTCCTCCCAGTCATCTCACTCCTACAAAGCAGAAG AGTACACTGAAGAGACAGAGGAAAGAGAGGAGAGCACCACGGGCTTTGACAAATCAAGACTGGGGACCAA AGACTTTGTGGGTCCAAGTGAAAGAGGAGGTGGCAGAGCTCGAGGAACCTTTCAGTTTCGAGCCAGAGGA AGAGGCTGGGGCAGAGGCAACTACTCTGGGAACAATAACAACAACAGCAACAACGATTTTCAAAAAAGAA ACCGGGAAGAGGAGTGGGACCCAGAGTACACACCCAAAAGCAAGAAGTATTACTTGCATGATGACCGTGA AGGCGAAGGCAGTGACAAGTGGGTGAGCCGGGGCCGGGGCCGAGGAGCCTTTCCTCGGGGTCGGGGCCGG TTCATGTTCCGGAAATCAAGTACCAGCCCCAAGTGGGCCCATGACAAGTTCAGTGGGGAGGAAGGGGAGA TTGAAGACGACGAGAGTGGGACAGAGAACCGAGAAGAGAAGGACAATATACAGCCCACAACCGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213342 protein sequence
Red=Cloning site Green=Tags(s) MSKTNKSKSGSRSSRSRSASRSRSRSFSKSRSRSRSLSRSRKRRLSSRSRSRSYSPAHNRERNHPRVYQN RDFRGHNRGYRRPYYFRGRNRGFYPWGQYNRGGYGNYRSNWQNYRQAYSPRRGRSRSRSPKRRSPSPRSR SHSRNSDKSSSDRSRRSSSSRSSSNHSRVESSKRKSAKEKKSSSKDSRPSQAAGDNQGDEAKEQTFSGGT SQDTKASESSKPWPDATYGTGSASRASAVSELSPRERSPALKSPLQSVVVRRRSPRPSPVPKPSPPLSST SQMGSTLPSGAGYQSGTHQGQFDHGSGSLSPSKKSPVGKSPPSTGSTYGSSQKEESAASGGAAYTKRYLE EQKTENGKDKEQKQTNTDKEKIKEKGSFSDTGLGDGKMKSDSFAPKTDSEKPFRGSQSPKRYKLRDDFEK KMADFHKEEMDDQDKDKAKGRKESEFDDEPKFMSKVIGANKNQEEEKSGKWEGLVYAPPGKEKQRKTEEL EEESFPERSKKEDRGKRSEGGHRGFVPEKNFRVTAYKAVQEKSSSPPPRKTSESRDKLGAKGDFPTGKSS FSITREAQVNVRMDSFDEDLARPSGLLAQERKLCRDLVHSNKKEQEFRSIFQHIQSAQSQRSPSELFAQH IVTIVHHVKEHHFGSSGMTLHERFTKYLKRGTEQEAAKNKKSPEIHRRIDISPSTFRKHGLAHDEMKSPR EPGYKAEGKYKDDPVDLRLDIERRKKHKERDLKRGKSRESVDSRDSSHSRERSAEKTEKTHKGSKKQKKH RRARDRSRSSSSSSQSSHSYKAEEYTEETEEREESTTGFDKSRLGTKDFVGPSERGGGRARGTFQFRARG RGWGRGNYSGNNNNNSNNDFQKRNREEEWDPEYTPKSKKYYLHDDREGEGSDKWVSRGRGRGAFPRGRGR FMFRKSSTSPKWAHDKFSGEEGEIEDDESGTENREEKDNIQPTTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005119 |
| ORF Size | 2865 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005119.4 |
| RefSeq Size | 4447 bp |
| RefSeq ORF | 2868 bp |
| Locus ID | 9967 |
| UniProt ID | Q9Y2W1 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 108.7 kDa |
| Gene Summary | Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition; the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex; however, it is not regarded as a stable Mediator complex subunit. Cooperatively with HELZ2, enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. May play a role in the terminal stage of adipocyte differentiation. Plays a role in the positive regulation of the circadian clock. Acts as a coactivator of the CLOCK-ARNTL/BMAL1 heterodimer and promotes its transcriptional activator activity and binding to circadian target genes (PubMed:24043798).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213342L1 | Lenti ORF clone of Human thyroid hormone receptor associated protein 3 (THRAP3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC213342L2 | Lenti ORF clone of Human thyroid hormone receptor associated protein 3 (THRAP3), mGFP tagged |
CNY 9,030.00 |
|
| RC213342L3 | Lenti ORF clone of Human thyroid hormone receptor associated protein 3 (THRAP3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC213342L4 | Lenti ORF clone of Human thyroid hormone receptor associated protein 3 (THRAP3), mGFP tagged |
CNY 9,030.00 |
|
| RG213342 | THRAP3 (tGFP-tagged) - Human thyroid hormone receptor associated protein 3 (THRAP3) |
CNY 7,890.00 |
|
| SC314985 | THRAP3 (untagged)-Human thyroid hormone receptor associated protein 3 (THRAP3) |
CNY 7,220.00 |
