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TBC1D4 (NM_014832) Human Tagged ORF Clone
CAT＃: RC212105
TBC1D4 (Myc-DDK-tagged)-Human TBC1 domain family, member 4 (TBC1D4)
ORF Plasmid: tGFP
"NM_014832" in other vectors (6)
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CNY 13,568.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AS160; NIDDM5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212105 representing NM_014832
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCCCCTGGAGCCCGAGCCGGGCGTCTCAG CTCAGCCCGGCCCCGGGAAGCCAAGCGATAAGCGGTTCCGGCTGTGGTACGTTGGGGGGTCGTGCCTGGA CCACAGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCCGAGATCCGCAGGCGCAGCCAGAAGCCCGAG GCGGGCGGCTGCGGGGCGCCGGCGGCCCGAGAGGTGATCCTGGTGCTCAGCGCGCCCTTCCTGCGTTGCG TCCCCGCGCCGGGCGCTGGGGCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGCGGTATT CATCTTCGAGCACAAGGCGCAGCATATCTCGCGCTTCATCCACAACAGCCACGACCTCACCTACTTTGCC TACCTGATCAAGGCGCAGCCCGACGACCCCGAGTCGCAGATGGCCTGCCACGTTTTCCGCGCCACAGACC CCAGCCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCGGCCATGAAAGAGGATGCCAA ACCCAGCAAAGATAATGAGGACGCCTTTTACAACTCTCAGAAGTTCGAAGTCCTGTACTGTGGAAAGGTG ACCGTGACCCACAAGAAGGCCCCCTCAAGCCTCATCGATGACTGCATGGAGAAGTTCAGCCTGCACGAAC AGCAGCGCCTGAAGATCCAAGGGGAGCAGCGCGGTCCGGACCCAGGAGAGGACCTGGCTGACTTGGAGGT GGTGGTGCCCGGGTCCCCCGGAGACTGCCTGCCGGAGGAGGCTGACGGCACCGACACCCACCTTGGCTTA CCTGCCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAGCGGATTTTGGAAGATT CTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCGGTGCAGCAGTGTCACCGGCGTGCAACGGAGAGTTCA CGAGGGCAGCCAGAAATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCACGTCCAGCCCTCGGAC TCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGGGCGATTTGAGATTAACCTTATCAGTCCAGACACTA AATCAGTTGTGCTAGAAAAGAATTTTAAAGATATCTCCTCTTGTTCTCAGGGTATAAAGCATGTGGATCA CTTTGGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATTTGTTATGTATTCCAGTGT GCCAGCGAATCTCTGGTTGATGAGGTAATGCTGACTCTGAAACAGGCCTTCAGTACGGCGGCTGCCCTGC AGAGTGCCAAGACGCAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCTTTGCATAAGCTCTGTGAAAG GATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATACAGAGGCATCTCTCATCACTGACAGATAAT GAGCAAGCTGACATCTTTGAAAGAGTTCAGAAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGAACTTG TGATTTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACACACGTGCACATCGGGGAAGGCCCTTC TACTATTTCAAATAGTACAATCCCAGAAAATGCAACAAGCAGTGGAAGGTTCAAACTTGACATTCTGAAA AATAAAGCTAAGAGATCCTTAACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAGCTAACAGAATGAGAG GTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTTGCTTCAGAGAAGGACTACTCACCAGG GGATTCTCCACCAGGGACACCGCCAGCGTCCCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAAGAGGAT TCCGACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGCCACCCACCTTCAAGCACAAAGAGAAAGC TGAATTTGCAGGATGGGAGGGCTCAGGGTGTGCGTTCCCCTCTGCTGAGGCAGAGCTCCAGTGAACAGTG CAGCAATCTTTCGTCAGTTCGACGCATGTACAAGGAGAGTAATTCTTCCTCCAGTCTTCCAAGTCTTCAC ACTTCCTTCTCTGCCCCTTCCTTCACTGCCCCCTCTTTCCTGAAAAGCTTTTACCAGAATTCAGGTAGAC TGTCCCCACAGTATGAAAATGAAATCAGACAAGACACTGCTTCAGAATCAAGTGATGGAGAAGGGAGAAA AAGGACCTCATCTACCTGCAGCAATGAGTCCCTAAGTGTGGGAGGAACCTCTGTCACTCCTCGCCGGATC TCCTGGCGGCAGCGCATTTTCCTCAGGGTTGCTTCTCCCATGAACAAATCTCCCTCAGCAATGCAACAGC AAGATGGATTGGACAGGAACGAGCTGCTGTCACTGTCCCCCCTCTCTCCAACCATGGAGGAGGAACCGCT GGTTGTATTCCTGTCTGGGGAGGATGACCCAGAAAAGATTGAAGAAAGAAAGAAATCAAAAGAACTGAGG AGCTTGTGGAGAAAAGCTATACACCAACAAATCTTGTTACTTCGAATGGAAAAAGAAAACCAGAAACTTG AAGGAGCAAGCAGAGATGAACTCCAGTCCAGAAAAGTTAAATTAGACTATGAAGAAGTTGGTGCATGTCA GAAAGAGGTCTTAATAACTTGGGATAAGAAGTTGTTAAACTGCAGAGCTAAAATCAGATGTGATATGGAA GATATTCATACTCTTCTTAAAGAAGGAGTTCCCAAAAGTCGACGAGGAGAAATTTGGCAGTTTCTGGCTT TACAGTACCGACTCAGACACAGATTGCCTAATAAACAACAGCCTCCTGACATATCCTATAAGGAACTTTT GAAGCAGCTCACTGCTCAGCAGCATGCGATTCTCGTGGATTTAGGAAGGACGTTTCCTACTCACCCTTAC TTTTCAGTACAGCTTGGGCCAGGACAGCTGTCACTGTTTAACCTCCTGAAAGCCTATTCTTTGCTGGACA AAGAAGTGGGATACTGTCAGGGGATCAGCTTTGTGGCTGGAGTCCTGCTTCTGCACATGAGTGAAGAGCA AGCCTTTGAAATGCTGAAATTCCTCATGTATGACCTCGGCTTCCGCAAGCAGTACAGACCTGACATGATG TCGCTGCAGATTCAAATGTACCAGCTGTCCAGGCTCCTTCATGACTATCACAGAGATCTCTACAATCACC TTGAAGAAAATGAAATCAGCCCCAGTCTTTATGCTGCCCCCTGGTTCCTCACATTGTTTGCCTCTCAGTT TTCATTAGGATTTGTAGCCAGAGTTTTTGATATTATTTTTCTTCAGGGAACTGAAGTTATATTCAAGGTT GCACTCAGCCTACTGAGCAGCCAAGAGACACTTATAATGGAATGTGAGAGCTTTGAAAATATTGTTGAGT TTCTTAAAAACACGCTACCTGATATGAATACCTCTGAAATGGAAAAAATTATTACCCAGGTTTTTGAGAT GGATATTTCTAAGCAGTTGCATGCCTATGAGGTGGAATATCATGTGCTACAGGATGAGCTTCAGGAATCT TCATATTCCTGTGAGGATAGTGAAACTTTGGAGAAGCTGGAGAGGGCCAATAGCCAACTGAAAAGACAAA ACATGGACCTCCTAGAAAAATTACAGGTAGCTCATACTAAAATCCAGGCCTTGGAATCAAACCTGGAAAA TCTTTTGACGAGAGAGACCAAAATGAAGTCTTTAATCCGGACCCTGGAACAAGAAAAAATGGCTTATCAA AAGACAGTGGAGCAACTCCGGAAGCTGCTGCCCGCGGATGCTCTAGTCAATTGTGACCTGTTGCTGAGAG ACCTAAACTGCAACCCTAACAACAAAGCCAAGATAGGAAATAAGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212105 representing NM_014832
Red=Cloning site Green=Tags(s) MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPE AGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFA YLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKV TVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGL PAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSD SEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQYICYVFQC ASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDN EQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPSTISNSTIPENATSSGRFKLDILK NKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEED SDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSNLSSVRRMYKESNSSSSLPSLH TSFSAPSFTAPSFLKSFYQNSGRLSPQYENEIRQDTASESSDGEGRKRTSSTCSNESLSVGGTSVTPRRI SWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLSLSPLSPTMEEEPLVVFLSGEDDPEKIEERKKSKELR SLWRKAIHQQILLLRMEKENQKLEGASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDME DIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLTAQQHAILVDLGRTFPTHPY FSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMM SLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVIFKV ALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQES SYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQ KTVEQLRKLLPADALVNCDLLLRDLNCNPNNKAKIGNKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014832 |
| ORF Size | 3897 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014832.5 |
| RefSeq Size | 5922 bp |
| RefSeq ORF | 3897 bp |
| Locus ID | 9882 |
| UniProt ID | O60343 |
| Domains | TBC, PID |
| MW | 146.4 kDa |
| Gene Summary | This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Constitutively Active CaMKKalpha Stimulates Skeletal Muscle Glucose Uptake in Insulin Resistant Mice In Vivo
,Hinkley, JM;Ferey, JL;Brault, JJ;Smith, CA;Gilliam, LA;Witczak, CA;,
Diabetes. 2013 Oct 7.
,PubMed ID 24101676
[TBC1D4]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212105L1 | Lenti ORF clone of Human TBC1 domain family, member 4 (TBC1D4), Myc-DDK-tagged |
CNY 15,968.00 |
|
| RC212105L2 | Lenti ORF clone of Human TBC1 domain family, member 4 (TBC1D4), mGFP tagged |
CNY 14,820.00 |
|
| RC212105L3 | Lenti ORF clone of Human TBC1 domain family, member 4 (TBC1D4), Myc-DDK-tagged |
CNY 14,820.00 |
|
| RC212105L4 | Lenti ORF clone of Human TBC1 domain family, member 4 (TBC1D4), mGFP tagged |
CNY 15,968.00 |
|
| RG212105 | TBC1D4 (tGFP-tagged) - Human TBC1 domain family, member 4 (TBC1D4) |
CNY 15,168.00 |
|
| SC309926 | TBC1D4 (untagged)-Human TBC1 domain family, member 4 (TBC1D4) |
CNY 9,048.00 |
