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iNOS (NOS2) (NM_000625) Human Tagged Lenti ORF Clone
CAT＃: RC211819L1
- LentiORF®
Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), Myc-DDK-tagged
"NM_000625" in other vectors (6)
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CNY 14,440.00
| Cited in 1 publication. |
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEP-NOS; INOS; NOS; NOS2A |
| Vector | pLenti-C-Myc-DDK |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC211819).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000625 |
| ORF Size | 3459 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000625.3 |
| RefSeq Size | 4221 bp |
| RefSeq ORF | 3462 bp |
| Locus ID | 4843 |
| UniProt ID | P35228 |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer |
| MW | 130.9 kDa |
| Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Linking of mPGES-1 and iNOS activates stem-like phenotype in EGFR-driven epithelial tumor cells
,Terzuoli, E;Finetti, F;Costanza, F;Giachetti, A;Ziche, M;Donnini, S;,
Nitric Oxide
,PubMed ID 28257996
[NOS2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211819 | NOS2 (Myc-DDK-tagged)-Human nitric oxide synthase 2, inducible (NOS2) |
CNY 12,040.00 |
|
| RC211819L2 | Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), mGFP tagged |
CNY 13,400.00 |
|
| RC211819L3 | Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), Myc-DDK-tagged |
CNY 14,440.00 |
|
| RC211819L4 | Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), mGFP tagged |
CNY 14,440.00 |
|
| RG211819 | NOS2 (tGFP-tagged) - Human nitric oxide synthase 2, inducible (NOS2) |
CNY 13,640.00 |
|
| SC300111 | NOS2 (untagged)-Human nitric oxide synthase 2, inducible (NOS2) |
CNY 12,048.00 |
