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BEST4 (NM_153274) Human Tagged ORF Clone
CAT＃: RC211033
- TrueORF®
BEST4 (Myc-DDK-tagged)-Human bestrophin 4 (BEST4)
ORF Plasmid: tGFP
"NM_153274" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | VMD2L2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211033 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGTTTCATACACTCTCAAAGTGGCGGAGGCCCGCTTCGGAGGTTTCTCTGGCCTGCTTCTCCGCT GGAGGGGAAGCATCTACAAGCTCCTCTACAAGGAATTCCTCCTCTTTGGGGCCTTGTACGCTGTGCTTAG CATCACCTACCGGCTGCTGCTGACCCAGGAGCAGAGGTACGTGTATGCTCAGGTGGCCCGGTACTGCAAC CGCTCAGCAGACCTCATTCCCTTGTCCTTTGTATTGGGTTTCTATGTGACTCTCGTGGTGAACCGCTGGT GGTCCCAGTACACAAGCATCCCGCTGCCAGACCAGCTGATGTGCGTCATCTCGGCTAGCGTGCACGGCGT GGACCAGCGGGGCCGCCTGCTGCGCCGCACCCTCATCCGCTACGCGAACCTGGCGTCCGTGCTGGTGCTG CGCTCGGTCAGCACCCGCGTGCTTAAGCGCTTCCCCACCATGGAGCACGTGGTGGATGCAGGTTTCATGT CCCAGGAAGAGAGGAAAAAGTTTGAGAGCCTGAAATCCGACTTCAACAAGTACTGGGTCCCCTGCGTCTG GTTCACCAACCTGGCGGCCCAGGCCCGGAGGGACGGGCGAATACGTGACGATATCGCTCTCTGTCTACTT TTGGAAGAGCTGAACAAGTACCGAGCCAAGTGCAGCATGCTATTCCACTATGACTGGATCAGCATCCCCC TCGTCTACACCCAAGTGGTGACCATAGCCGTCTACTCTTTCTTTGCCCTCTCCCTGGTTGGCCGCCAGTT TGTGGAGCCAGAGGCAGGGGCTGCCAAACCTCAGAAGCTTCTGAAGCCAGGCCAGGAGCCAGCCCCAGCC CTGGGAGACCCGGACATGTACGTGCCTCTCACCACTCTGCTGCAGTTCTTCTTCTATGCTGGCTGGCTCA AGGTGGCTGAACAGATCATCAACCCATTTGGTGAGGATGATGACGACTTTGAGACAAATCAGCTCATAGA CCGCAACTTGCAGGTGTCCCTGCTATCCGTGGACGAAATGTACCAGAACCTTCCCCCCGCTGAGAAGGAC CAGTACTGGGATGAGGACCAGCCGCAGCCACCCTACACTGTGGCCACGGCGGCCGAGTCTCTGCGGCCCT CATTCCTGGGCTCCACCTTCAACCTGCGCATGAGCGACGACCCTGAGCAGAGCCTGCAGGTGGAGGCGTC CCCCGGATCTGGTCGGCCCGCGCCCGCCGCGCAGACCCCGTTGCTCGGCCGCTTCCTGGGCGTAGGGGCG CCCTCCCCGGCCATCAGCCTCCGGAACTTCGGCCGCGTGCGAGGCACCCCCCGCCCCCCGCATCTGCTGC GCTTCCGGGCGGAGGAGGGCGGCGACCCCGAGGCCGCAGCCCGCATCGAGGAGGAATCGGCGGAGTCCGG GGACGAGGCCCTGGAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211033 protein sequence
Red=Cloning site Green=Tags(s) MTVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQEQRYVYAQVARYCN RSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQRGRLLRRTLIRYANLASVLVL RSVSTRVLKRFPTMEHVVDAGFMSQEERKKFESLKSDFNKYWVPCVWFTNLAAQARRDGRIRDDIALCLL LEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPA LGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVSLLSVDEMYQNLPPAEKD QYWDEDQPQPPYTVATAAESLRPSFLGSTFNLRMSDDPEQSLQVEASPGSGRPAPAAQTPLLGRFLGVGA PSPAISLRNFGRVRGTPRPPHLLRFRAEEGGDPEAAARIEEESAESGDEALEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153274 |
| ORF Size | 1419 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153274.3 |
| RefSeq Size | 2096 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 266675 |
| UniProt ID | Q8NFU0 |
| Protein Families | Ion Channels: Other, Transmembrane |
| MW | 53.5 kDa |
| Gene Summary | This gene is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region rich in aromatic residues, including an invariant arg-phe-pro motif. Mutation in one of the family members (bestrophin 1) is associated with vitelliform macular dystrophy. The bestrophin 4 gene is predominantly expressed in the colon. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Autoantibody discovery across monogenic, acquired, and COVID-19-associated autoimmunity with scalable PhIP-seq
,null,
eLife
,PubMed ID 36300623
[BEST4]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211033L3 | Lenti ORF clone of Human bestrophin 4 (BEST4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211033L4 | Lenti ORF clone of Human bestrophin 4 (BEST4), mGFP tagged |
CNY 5,890.00 |
|
| RG211033 | BEST4 (tGFP-tagged) - Human bestrophin 4 (BEST4) |
CNY 4,370.00 |
|
| SC310350 | BEST4 (untagged)-Human bestrophin 4 (BEST4) |
CNY 3,656.00 |
