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SIPA1 (NM_006747) Human Tagged ORF Clone
CAT＃: RC210305
- TrueORF®
SIPA1 (Myc-DDK-tagged)-Human signal-induced proliferation-associated 1 (SIPA1), transcript variant 2
ORF Plasmid: tGFP
"NM_006747" in other vectors (5)
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CNY 10,360.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SPA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210305 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCATGTGGGCCGGCGGTGTGGGGAGCCCTCGGCGGGGCATGGCCCCTGCGTCCACAGATGACCTCT TTGCCCGCAAGCTGCGCCAGCCAGCAAGGCCCCCGCTGACACCGCACACCTTCGAGCCGAGGCCAGTCCG GGGCCCACTCCTGCGCAGCGGCAGCGATGCAGGCGAGGCCAGGCCCCCCACGCCAGCCAGCCCCCGTGCC CGTGCCCACAGCCACGAAGAGGCCAGCCGACCTGCAGCCACTTCCACCCGGCTCTTCACTGACCCGCTGG CACTGCTGGGGCTGCCAGCAGAGGAACCAGAGCCTGCCTTCCCACCAGTGCTTGAGCCTCGATGGTTTGC CCACTATGACGTGCAAAGCCTGCTCTTTGATTGGGCTCCGAGGTCTCAGGGGATGGGGAGCCACTCAGAG GCCAGCTCTGGGACCCTGGCTTCAGCCGAGGACCAGGCTGCCAGCTCGGACCTGCTGCATGGGGCACCTG GCTTTGTGTGTGAGCTCGGGGGTGAGGGTGAGCTAGGCCTGGGTGGACCAGCATCCCCACCTGTGCCCCC TGCACTGCCCAACGCGGCCGTGTCCATCCTGGAGGAGCCACAGAACCGAACCTCGGCCTACAGCCTGGAG CACGCAGACCTGGGTGCTGGCTACTACCGCAAATACTTCTATGGCAAAGAACATCAGAACTTCTTCGGGA TGGACGAGTCGCTGGGCCCGGTGGCAGTGAGCCTGCGGCGGGAGGAGAAGGAGGGCAGCGGAGGGGGCAC CCTGCACAGCTACCGCGTCATCGTGCGGACCACGCAGCTCCGGACACTCCGTGGCACCATCTCGGAGGAC GCGCTGCCGCCGGGGCCCCCACGGGGTCTGTCCCCAAGGAAACTTCTGGAGCACGTGGCGCCGCAGCTGA GCCCCAGCTGCTTGCGCCTGGGCTCAGCTTCACCCAAGGTACCACGGACGCTGCTCACACTGGATGAGCA AGTGCTGAGCTTCCAACGCAAGGTGGGCATCCTGTACTGCCGGGCGGGCCAGGGCTCGGAGGAGGAGATG TACAACAACCAGGAGGCGGGACCGGCCTTCATGCAGTTTCTCACCTTGCTGGGCGATGTGGTGCGGCTCA AAGGCTTTGAGAGTTACCGGGCCCAGCTAGACACCAAAACGGATTCCACAGGCACGCACTCCCTCTACAC CACATACCAGGACCACGAGATCATGTTCCACGTGTCCACGATGCTGCCTTACACCCCTAATAACCAGCAG CAGCTCCTCCGGAAGCGCCACATTGGCAACGACATTGTGACCATCGTGTTCCAGGAGCCTGGCAGCAAGC CCTTCTGCCCCACCACCATCCGCTCGCACTTCCAGCACGTGTTCCTAGTGGTGCGGGCACACACACCCTG CACGCCACACACCACCTACAGGGTGGCCGTGAGCCGCACCCAGGACACCCCTGCCTTCGGGCCAGCTCTG CCTGCTGGCGGAGGCCCCTTCGCAGCCAACGCCGACTTCCGGGCCTTCCTGCTGGCCAAAGCGCTGAATG GTGAGCAGGCGGCCGGCCACGCGCGCCAGTTCCACGCCATGGCCACGCGCACCCGCCAGCAGTACCTGCA AGACCTGGCCACCAACGAGGTGACCACTACGTCGCTGGACTCGGCTTCACGCTTCGGCCTGCCCTCCCTG GGTGGGAGGCGCCGGGCGGCCCCTCGGGGCCCAGGCGCCGAGCTGCAGGCAGCGGGCTCACTGGTGTGGG GAGTGCGCGCGGCGCCCGGGGCGCGGGTCGCCGCCGGGGCTCAGGCGAGCGGCCCCGAAGGCATCGAGGT GCCCTGCCTGCTGGGCATCTCGGCCGAGGCTCTGGTGCTGGTGGCGCCGCGCGACGGCCGCGTAGTGTTC AATTGCGCCTGTCGCGACGTGCTGGCCTGGACCTTCTCCGAGCAGCAGCTGGACCTGTACCACGGCCGCG GGGAGGCGATCACGCTGCGCTTCGACGGGTCCCCCGGCCAAGCCGTGGGCGAGGTGGTGGCGCGCCTGCA GCTGGTGAGCCGTGGCTGCGAGACCCGCGAGCTGGCGCTGCCCCGCGACGGTCAAGGCCGCCTGGGCTTC GAGGTGGACGCCGAGGGATTCGTCACGCACGTGGAGCGCTTCACATTCGCCGAGACGGCGGGGCTGCGGC CCGGGGCGCGCCTCCTGCGCGTGTGCGGCCAGACGCTGCCCAGCCTCCGGCCCGAGGCCGCTGCCCAGCT CCTGCGCTCGGCGCCCAAGGTCTGCGTCACCGTCCTGCCCCCCGACGAGAGCGGCCGGCCCCGCAGGAGT TTTTCGGAGCTGTACACGCTGTCGCTGCAGGAGCCTAGCCGGCGGGGGGCCCCAGATCCTGTGCAGGATG AGGTCCAGGGGGTGACCCTGCTGCCCACCACAAAGCAGCTGCTGCACCTGTGCCTGCAAGATGGTGGCAG TCCTCCAGGGCCTGGGGATCTGGCCGAGGAGAGGACTGAGTTCCTGCACAGCCAGAACTCGCTGTCACCA CGCAGCTCTCTGTCGGATGAGGCCCCAGTCCTGCCCAACACCACCCCGGACCTCCTCCTGGCCACCACAG CCAAGCCATCAGTACCCAGTGCTGACAGTGAGACACCCCTGACCCAGGACAGGCCAGGCAGTCCCAGTGG CTCTGAGGACAAGGGCAACCCGGCGCCGGAGCTGAGGGCCTCCTTTCTGCCACGTACCTTGTCTCTGCGG AACTCCATCAGCAGGATCATGTCGGAGGCGGGCAGTGGGACCCTGGAGGACGAGTGGCAGGCCATCTCGG AGATTGCCTCTACTTGCAACACCATTCTGGAGTCGCTGTCCCGAGAGGGACAGCCCATCCCAGAGAGTGG AGACCCTAAGGGAACTCCAAAATCTGATGCTGAGCCAGAGCCTGGGAACCTCTCAGAGAAGGTCTCTCAC TTGGAGTCCATGCTCAGGAAGCTGCAGGAGGACCTGCAGAAGGAGAAGGCGGACAGGGCGGCCCTGGAGG AGGAGGTGCGGAGCCTGAGACACAACAACCGGCGGCTGCAGGCGGAGTCTGAGAGTGCAGCCACACGCCT CCTCCTGGCCTCCAAGCAGCTGGGCTCACCCACCGCCGACCTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210305 protein sequence
Red=Cloning site Green=Tags(s) MPMWAGGVGSPRRGMAPASTDDLFARKLRQPARPPLTPHTFEPRPVRGPLLRSGSDAGEARPPTPASPRA RAHSHEEASRPAATSTRLFTDPLALLGLPAEEPEPAFPPVLEPRWFAHYDVQSLLFDWAPRSQGMGSHSE ASSGTLASAEDQAASSDLLHGAPGFVCELGGEGELGLGGPASPPVPPALPNAAVSILEEPQNRTSAYSLE HADLGAGYYRKYFYGKEHQNFFGMDESLGPVAVSLRREEKEGSGGGTLHSYRVIVRTTQLRTLRGTISED ALPPGPPRGLSPRKLLEHVAPQLSPSCLRLGSASPKVPRTLLTLDEQVLSFQRKVGILYCRAGQGSEEEM YNNQEAGPAFMQFLTLLGDVVRLKGFESYRAQLDTKTDSTGTHSLYTTYQDHEIMFHVSTMLPYTPNNQQ QLLRKRHIGNDIVTIVFQEPGSKPFCPTTIRSHFQHVFLVVRAHTPCTPHTTYRVAVSRTQDTPAFGPAL PAGGGPFAANADFRAFLLAKALNGEQAAGHARQFHAMATRTRQQYLQDLATNEVTTTSLDSASRFGLPSL GGRRRAAPRGPGAELQAAGSLVWGVRAAPGARVAAGAQASGPEGIEVPCLLGISAEALVLVAPRDGRVVF NCACRDVLAWTFSEQQLDLYHGRGEAITLRFDGSPGQAVGEVVARLQLVSRGCETRELALPRDGQGRLGF EVDAEGFVTHVERFTFAETAGLRPGARLLRVCGQTLPSLRPEAAAQLLRSAPKVCVTVLPPDESGRPRRS FSELYTLSLQEPSRRGAPDPVQDEVQGVTLLPTTKQLLHLCLQDGGSPPGPGDLAEERTEFLHSQNSLSP RSSLSDEAPVLPNTTPDLLLATTAKPSVPSADSETPLTQDRPGSPSGSEDKGNPAPELRASFLPRTLSLR NSISRIMSEAGSGTLEDEWQAISEIASTCNTILESLSREGQPIPESGDPKGTPKSDAEPEPGNLSEKVSH LESMLRKLQEDLQKEKADRAALEEEVRSLRHNNRRLQAESESAATRLLLASKQLGSPTADLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006747 |
| ORF Size | 3126 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006747.4 |
| RefSeq Size | 3513 bp |
| RefSeq ORF | 3129 bp |
| Locus ID | 6494 |
| UniProt ID | Q96FS4 |
| Domains | Rap_GAP, PDZ |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS |
| Protein Pathways | Leukocyte transendothelial migration |
| MW | 112.1 kDa |
| Gene Summary | The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210305L3 | Lenti-ORF clone of SIPA1 (Myc-DDK-tagged)-Human signal-induced proliferation-associated 1 (SIPA1), transcript variant 2 |
CNY 12,260.00 |
|
| RC210305L4 | Lenti-ORF clone of SIPA1 (mGFP-tagged)-Human signal-induced proliferation-associated 1 (SIPA1), transcript variant 2 |
CNY 12,260.00 |
|
| RG210305 | SIPA1 (tGFP-tagged) - Human signal-induced proliferation-associated 1 (SIPA1), transcript variant 2 |
CNY 11,400.00 |
|
| SC109764 | SIPA1 (untagged)-Human signal-induced proliferation-associated 1 (SIPA1), transcript variant 2 |
CNY 7,264.00 |
|
| SC322777 | SIPA1 (untagged)-Human signal-induced proliferation-associated 1 (SIPA1), transcript variant 2 |
CNY 7,264.00 |
