SLC10A1 (NM_003049) Human Tagged ORF Clone
CAT#: RC210241
SLC10A1 (Myc-DDK-tagged)-Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1)
ORF Plasmid: tGFP
"NM_003049" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
Cited in 8 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FHCA2; NTCP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210241 representing NM_003049
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCCACAACGCGTCTGCCCCATTCAACTTCACCCTGCCACCCAACTTTGGCAAGCGCCCCACAG ACCTGGCACTGAGCGTCATCCTGGTGTTCATGTTGTTCTTCATCATGCTCTCGCTGGGCTGCACCATGGA GTTCAGCAAGATCAAGGCTCACTTATGGAAGCCTAAAGGGCTGGCCATCGCCCTGGTGGCACAGTATGGC ATCATGCCCCTCACGGCCTTTGTGCTGGGCAAGGTCTTCCGGCTGAAGAACATTGAGGCACTGGCCATCT TGGTCTGTGGCTGCTCACCTGGAGGGAACCTGTCCAATGTCTTCAGTCTGGCCATGAAGGGGGACATGAA CCTCAGCATTGTGATGACCACCTGCTCCACCTTCTGTGCCCTTGGCATGATGCCTCTCCTCCTGTACATC TACTCCAGGGGGATCTATGATGGGGACCTGAAGGACAAGGTGCCCTATAAAGGCATCGTGATATCACTGG TCCTGGTTCTCATTCCTTGCACCATAGGGATCGTCCTCAAATCCAAACGGCCACAATACATGCGCTATGT CATCAAGGGAGGGATGATCATCATTCTCTTGTGCAGTGTGGCCGTCACAGTTCTCTCTGCCATCAATGTG GGGAAGAGCATCATGTTTGCCATGACACCACTCTTGATTGCCACCTCCTCCCTGATGCCTTTTATTGGCT TTCTGCTGGGTTATGTTCTCTCTGCTCTCTTCTGCCTCAATGGACGGTGCAGACGCACTGTCAGCATGGA GACTGGATGCCAAAATGTCCAACTCTGTTCCACCATCCTCAATGTGGCCTTTCCACCTGAAGTCATTGGA CCACTTTTCTTCTTTCCCCTCCTCTACATGATTTTCCAGCTTGGAGAAGGGCTTCTCCTCATTGCCATAT TTTGGTGCTATGAGAAATTCAAGACTCCCAAGGATAAAACAAAAATGATCTACACAGCTGCCACAACTGA AGAAACAATTCCAGGAGCTCTGGGAAATGGCACCTACAAAGGGGAGGACTGCTCCCCTTGCACAGCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC210241 representing NM_003049
Red=Cloning site Green=Tags(s) MEAHNASAPFNFTLPPNFGKRPTDLALSVILVFMLFFIMLSLGCTMEFSKIKAHLWKPKGLAIALVAQYG IMPLTAFVLGKVFRLKNIEALAILVCGCSPGGNLSNVFSLAMKGDMNLSIVMTTCSTFCALGMMPLLLYI YSRGIYDGDLKDKVPYKGIVISLVLVLIPCTIGIVLKSKRPQYMRYVIKGGMIIILLCSVAVTVLSAINV GKSIMFAMTPLLIATSSLMPFIGFLLGYVLSALFCLNGRCRRTVSMETGCQNVQLCSTILNVAFPPEVIG PLFFFPLLYMIFQLGEGLLLIAIFWCYEKFKTPKDKTKMIYTAATTEETIPGALGNGTYKGEDCSPCTA SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003049 |
ORF Size | 1047 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003049.4 |
RefSeq Size | 1580 bp |
RefSeq ORF | 1050 bp |
Locus ID | 6554 |
UniProt ID | Q14973 |
Domains | SBF |
Protein Families | Druggable Genome, Transmembrane |
MW | 37.9 kDa |
Gene Summary | The protein encoded by this gene belongs to the sodium/bile acid cotransporter family, which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen, bile duct and kidney, and the liver-specific sodium/bile acid cotransporter, represented by this protein, that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism, hence this protein is important for cholesterol homeostasis. [provided by RefSeq, Oct 2011] |
Citations (8)
The use of this cDNA Clones has been cited in the following citations: |
---|
All- trans Retinoic Acid Inhibits Hepatitis B Virus Replication by Downregulating HBx Levels via Siah-1-Mediated Proteasomal Degradation
,null,
Viruses
,PubMed ID 37515144
[SLC10A1]
|
Hepatitis B Virus X Protein Stimulates Hepatitis C Virus (HCV) Replication by Protecting HCV Core Protein from E6AP-Mediated Proteasomal Degradation
,null,
Microbiology Spectrum
,PubMed ID 36374094
[SLC10A1]
|
Hepatitis B virus X protein and hepatitis C virus core protein cooperate to repress E-cadherin expression via DNA methylation
,null,
Heliyon
,PubMed ID 35832344
[SLC10A1]
|
HBV induces liver fibrosis via the TGF-β1/miR-21-5p pathway
,Li, W;Yu, X;Chen, X;Wang, Z;Yin, M;Zhao, Z;Zhu, C;,
Experimental and therapeutic medicine
,PubMed ID 33456536
[SLC10A1]
|
Hepatitis B virus X protein stimulates cell growth by downregulating p16 levels via PA28γ-mediated proteasomal degradation
,Cha, S;Jang, KL;,
J. Gen. Virol.
,PubMed ID 32568029
[SLC10A1]
|
Hepatitis B Virus X Protein Stimulates Virus Replication Via DNA Methylation of the C-1619 in Covalently Closed Circular DNA
,Lee, H;Jeong, H;Lee, SY;Kim, SS;Jang, KL;,
Mol. Cells
,PubMed ID 30518174
[SLC10A1]
|
A novel bioluminescence-based method to investigate uptake of bile acids in living cells
,Ticho, AL;Lee, H;Gill, RK;Dudeja, PK;Saksena, S;Lee, D;Alrefai, WA;,
Am. J. Physiol. Gastrointest. Liver Physiol.
,PubMed ID 29927324
[SLC10A1]
|
Characterization of the Anti-HCV Activities of the New Cyclophilin Inhibitor STG-175
,Gallay, PA;Chatterji, U;Bobardt, MD;Long, Z;Zhang, S;Su, Z;,
PLoS ONE
,PubMed ID 27104614
[SLC10A1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210241L1 | Lenti ORF clone of Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1), Myc-DDK-tagged |
CNY 7,888.00 |
|
RC210241L2 | Lenti ORF clone of Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1), mGFP tagged |
CNY 7,888.00 |
|
RC210241L3 | Lenti ORF clone of Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1), Myc-DDK-tagged |
CNY 7,888.00 |
|
RC210241L4 | Lenti ORF clone of Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1), mGFP tagged |
CNY 7,888.00 |
|
RG210241 | SLC10A1 (tGFP-tagged) - Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1) |
CNY 7,088.00 |
|
SC118232 | SLC10A1 (untagged)-Human solute carrier family 10 (sodium/bile acid cotransporter family), member 1 (SLC10A1) |
CNY 5,488.00 |
其它SLC10A1产品