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NOP2 (NM_006170) Human Tagged ORF Clone
CAT＃: RC209553
NOP2 (Myc-DDK-tagged)-Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 1
ORF Plasmid: tGFP
"NM_006170" in other vectors (4)
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CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NOL1; NOP120; NSUN1; p120 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209553 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGCAAGTTGGACCCTACGAAGGAGAAGCGGGGGCCAGGCCGAAAGGCCCGGAAGCAGAAGGGTG CCGAGACAGAACTCGTCAGATTCTTGCCTGCAGTAAGTGACGAAAATTCCAAGAGGCTGTCTAGTCGTGC TCGAAAGAGGGCAGCCAAGAGGAGATTGGGCTCTGTTGAAGCCCCTAAGACAAATAAGTCTCCTGAGGCC AAACCATTGCCTGGAAAGCTACCAAAAGGAGCTGTCCAGACAGCTGGTAAGAAGGGACCCCAGTCCCTAT TTAATGCTCCTCGAGGCAAGAAGCGCCCAGCACCTGGCAGTGATGAGGAAGAGGAGGAGGAAGACTCTGA AGAAGATGGTATGGTGAACCACGGGGACCTCTGGGGCTCCGAGGACGATGCTGATACGGTAGATGACTAT GGAGCTGACTCCAACTCTGAGGATGAGGAGGAAGGTGAAGCGTTGCTGCCCATTGAAAGAGCTGCTCGGA AGCAGAAGGCCCGGGAAGCTGCTGCTGGGATCCAGTGGAGTGAAGAGGAGACCGAGGACGAGGAGGAAGA GAAAGAAGTGACCCCTGAGTCAGGCCCCCCAAAGGTGGAAGAGGCAGATGGGGGCCTGCAGATCAATGTG GATGAGGAACCATTTGTGCTGCCCCCTGCTGGGGAGATGGAGCAGGATGCCCAGGCTCCAGACCTGCAAC GAGTTCACAAGCGGATCCAGGATATTGTGGGAATTCTGCGTGATTTTGGGGCTCAGCGGGAGGAAGGGCG GTCTCGTTCTGAATACCTGAACCGGCTCAAGAAGGATCTGGCCATTTACTACTCCTATGGAGACTTCCTG CTTGGCAAGCTCATGGACCTCTTCCCTCTGTCTGAGCTGGTGGAGTTCTTAGAAGCTAATGAGGTGCCTC GGCCCGTCACCCTCCGGACCAATACCTTGAAAACCCGACGCCGAGACCTTGCACAGGCTCTAATCAATCG TGGGGTTAACCTGGATCCCCTGGGCAAGTGGTCAAAGACTGGACTAGTGGTGTATGATTCTTCTGTGCCC ATTGGTGCTACCCCCGAGTACCTGGCTGGGCACTACATGCTGCAGGGAGCCTCCAGCATGTTGCCCGTCA TGGCCTTGGCACCCCAGGAACATGAGCGGATCCTGGACATGTGTTGTGCCCCTGGAGGAAAGACCAGCTA CATGGCCCAGCTGATGAAGAACACGGGTGTGATCCTTGCCAATGACGCCAATGCTGAGCGGCTCAAGAGT GTTGTGGGCAACTTGCATCGGCTGGGAGTCACCAACACCATTATCAGCCACTATGATGGGCGCCAGTTCC CCAAGGTGGTGGGGGGCTTTGACCGAGTACTGCTGGATGCTCCCTGCAGTGGCACTGGGGTCATCTCCAA GGATCCAGCCGTGAAGACTAACAAGGATGAGAAGGACATCCTGCGCTGTGCTCACCTCCAGAAGGAGTTG CTCCTGAGTGCTATTGACTCTGTCAATGCGACCTCCAAGACAGGAGGCTACCTGGTTTACTGCACCTGTT CTATCACAGTAGAAGAGAATGAGTGGGTGGTAGACTATGCTCTGAAAAAGAGGAATGTGCGACTGGTGCC CACGGGCCTAGACTTTGGCCAGGAAGGTTTTACCCGCTTTCGAGAAAGGCGCTTCCACCCCAGTCTGCGT TCTACCCGACGCTTCTACCCTCATACCCACAATATGGATGGGTTCTTCATTGCCAAGTTCAAGAAATTTT CCAATTCTATCCCTCAGTCCCAGACAGGAAATTCTGAAACAGCCACACCTACAAATGTAGACTTGCCTCA GGTCATCCCCAAGTCTGAGAACAGCAGCCAGCCAGCCAAGAAAGCCAAGGGGGCTGCAAAGACAAAGCAG CAGCTGCAGAAACAGCAACATCCCAAGAAGGCCTCCTTCCAGAAGCTGAATGGCATCTCCAAAGGGGCAG ACTCAGAATTGTCCACTGTACCTTCTGTCACAAAGACCCAAGCTTCCTCCAGCTTCCAGGATAGCAGTCA GCCAGCTGGAAAAGCCGAAGGGATCAGGGAGCCAAAGGTGACTGGGAAGCTAAAGCAACGATCACCTAAA TTACAGTCCTCCAAGAAAGTTGCTTTCCTCAGGCAGAATGCCCCTCCCAAGGGCACAGACACACAAACAC CGGCTGTGTTATCCCCATCCAAGACTCAGGCCACCCTGAAACCTAAGGACCATCATCAGCCCCTTGGAAG GGCCAAGGGGGTTGAGAAGCAGCAGTTGCCAGAGCAGCCTTTTGAGAAAGCTGCCTTCCAGAAACAGAAT GATACCCCCAAGGGGCCTCAGCCTCCCACTGTGTCTCCCATCCGTTCCAGCCGCCCCCCACCAGCAAAGA GGAAGAAATCTCAGTCCAGGGGCAACAGCCAGCTGCTGCTATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209553 protein sequence
Red=Cloning site Green=Tags(s) MGRKLDPTKEKRGPGRKARKQKGAETELVRFLPAVSDENSKRLSSRARKRAAKRRLGSVEAPKTNKSPEA KPLPGKLPKGAVQTAGKKGPQSLFNAPRGKKRPAPGSDEEEEEEDSEEDGMVNHGDLWGSEDDADTVDDY GADSNSEDEEEGEALLPIERAARKQKAREAAAGIQWSEEETEDEEEEKEVTPESGPPKVEEADGGLQINV DEEPFVLPPAGEMEQDAQAPDLQRVHKRIQDIVGILRDFGAQREEGRSRSEYLNRLKKDLAIYYSYGDFL LGKLMDLFPLSELVEFLEANEVPRPVTLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKTGLVVYDSSVP IGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYMAQLMKNTGVILANDANAERLKS VVGNLHRLGVTNTIISHYDGRQFPKVVGGFDRVLLDAPCSGTGVISKDPAVKTNKDEKDILRCAHLQKEL LLSAIDSVNATSKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFRERRFHPSLR STRRFYPHTHNMDGFFIAKFKKFSNSIPQSQTGNSETATPTNVDLPQVIPKSENSSQPAKKAKGAAKTKQ QLQKQQHPKKASFQKLNGISKGADSELSTVPSVTKTQASSSFQDSSQPAGKAEGIREPKVTGKLKQRSPK LQSSKKVAFLRQNAPPKGTDTQTPAVLSPSKTQATLKPKDHHQPLGRAKGVEKQQLPEQPFEKAAFQKQN DTPKGPQPPTVSPIRSSRPPPAKRKKSQSRGNSQLLLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006170 |
| ORF Size | 2424 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006170.4 |
| RefSeq Size | 2704 bp |
| RefSeq ORF | 2427 bp |
| Locus ID | 4839 |
| UniProt ID | P46087 |
| Domains | Nol1_Nop2_Sun |
| MW | 89 kDa |
| Gene Summary | Involved in ribosomal large subunit assembly (PubMed:24120868). S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA (Probable). May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209553L3 | Lenti ORF clone of Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC209553L4 | Lenti ORF clone of Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG209553 | NOP2 (tGFP-tagged) - Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 1 |
CNY 6,370.00 |
|
| SC314823 | NOP2 (untagged)-Human NOP2 nucleolar protein homolog (yeast) (NOP2), transcript variant 1 |
CNY 7,220.00 |
