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VGF (NM_003378) Human Tagged ORF Clone
CAT＃: RC209477
VGF (Myc-DDK-tagged)-Human VGF nerve growth factor inducible (VGF)
ORF Plasmid: tGFP
"NM_003378" in other vectors (6)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SCG7; SgVII |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209477 representing NM_003378
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGCCCTCAGATTGTCGGCTTCCGCCCTCTTCTGCCTTCTGCTGATCAACGGGTTAGGGGCAGCAC CCCCTGGTCGCCCTGAGGCGCAGCCTCCTCCTCTCAGCTCTGAGCATAAAGAGCCGGTAGCCGGGGACGC AGTGCCCGGGCCAAAGGATGGCAGCGCCCCAGAGGTCCGAGGCGCTCGGAATTCCGAGCCGCAGGACGAG GGAGAGCTTTTCCAGGGCGTGGATCCCCGGGCGCTGGCCGCGGTGCTGCTGCAGGCACTCGACCGTCCCG CCTCACCCCCGGCACCAAGCGGCTCCCAGCAGGGGCCGGAGGAAGAAGCAGCTGAAGCTCTGCTGACCGA GACCGTGCGCAGCCAGACCCACAGCCTCCCGGCGCCGGAGAGCCCGGAGCCCGCGGCTCCGCCTCGCCCT CAGACTCCGGAGAATGGGCCCGAGGCGAGCGATCCCTCCGAGGAGCTCGAGGCGCTAGCGTCCCTGCTCC AGGAACTGCGAGATTTCAGTCCAAGTAGCGCCAAGCGCCAGCAGGAGACGGCGGCAGCAGAGACGGAAAC CCGCACGCACACGCTGACCCGAGTGAATCTGGAGAGCCCGGGGCCAGAGCGCGTATGGCGCGCTTCCTGG GGAGAGTTCCAGGCGCGTGTCCCGGAGCGCGCGCCCCTGCCGCCCCCGGCCCCCTCTCAATTCCAGGCGC GTATGCCCGACAGCGGGCCCCTTCCCGAAACCCACAAGTTCGGGGAAGGAGTGTCCTCCCCCAAAACACA CCTAGGCGAGGCATTGGCACCCCTGTCCAAGGCGTACCAAGGCGTGGCCGCCCCGTTCCCCAAGGCGCGC CGGCCGGAGAGCGCACTCCTGGGCGGCTCCGAGGCGGGCGAGCGCCTTCTCCAGCAAGGGCTGGCGCAGG TGGAGGCCGGGCGGCGGCAGGCGGAGGCCACGCGGCAGGCCGCGGCGCAGGAAGAGCGGCTGGCCGACCT CGCCTCGGACCTGCTGCTCCAGTATTTGCTGCAGGGCGGGGCCCGGCAGCGCGGCCTCGGGGGTCGGGGG CTGCAGGAGGCGGCGGAGGAGCGAGAGAGTGCAAGGGAGGAGGAGGAGGCGGAGCAGGAGAGACGCGGCG GGGAGGAGAGGGTGGGGGAAGAGGATGAGGAGGCGGCCGAGGCGGAGGCAGAGGCGGAGGAGGCGGAGAG GGCGCGGCAGAACGCGCTCCTGTTCGCGGAGGAGGAGGACGGGGAAGCCGGCGCCGAGGACAAGCGCTCC CAGGAGGAGACGCCGGGCCACCGGCGGAAGGAGGCCGAGGGGACAGAGGAGGGCGGGGAGGAGGAGGACG ACGAGGAGATGGATCCGCAGACGATCGACAGCCTCATTGAGCTGTCCACCAAACTCCACCTGCCAGCGGA CGACGTGGTCAGCATCATCGAGGAGGTGGAGGAGAAGCGGAAGCGGAAGAAGAACGCCCCTCCCGAGCCC GTGCCGCCCCCCCGTGCCGCCCCCGCCCCCACCCACGTCCGCTCCCCGCAGCCCCCGCCCCCCGCCCCCG CTCCCGCACGAGACGAGCTGCCGGACTGGAACGAGGTGCTCCCGCCCTGGGATCGGGAGGAGGACGAGGT GTACCCGCCAGGGCCGTACCACCCTTTCCCCAACTACATCCGGCCGCGGACACTGCAGCCGCCCTCGGCC TTGCGCCGCCGCCACTACCACCACGCCTTGCCGCCTTCGCGCCACTATCCCGGCCGGGAGGCCCAGGCGC GGCGCGCGCAGGAGGAGGCGGAGGCGGAGGAGCGCCGGCTGCAGGAGCAGGAGGAGCTGGAGAATTACAT CGAGCACGTGCTGCTCCGGCGCCCG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC209477 representing NM_003378
Red=Cloning site Green=Tags(s) MKALRLSASALFCLLLINGLGAAPPGRPEAQPPPLSSEHKEPVAGDAVPGPKDGSAPEVRGARNSEPQDE GELFQGVDPRALAAVLLQALDRPASPPAPSGSQQGPEEEAAEALLTETVRSQTHSLPAPESPEPAAPPRP QTPENGPEASDPSEELEALASLLQELRDFSPSSAKRQQETAAAETETRTHTLTRVNLESPGPERVWRASW GEFQARVPERAPLPPPAPSQFQARMPDSGPLPETHKFGEGVSSPKTHLGEALAPLSKAYQGVAAPFPKAR RPESALLGGSEAGERLLQQGLAQVEAGRRQAEATRQAAAQEERLADLASDLLLQYLLQGGARQRGLGGRG LQEAAEERESAREEEEAEQERRGGEERVGEEDEEAAEAEAEAEEAERARQNALLFAEEEDGEAGAEDKRS QEETPGHRRKEAEGTEEGGEEEDDEEMDPQTIDSLIELSTKLHLPADDVVSIIEEVEEKRKRKKNAPPEP VPPPRAAPAPTHVRSPQPPPPAPAPARDELPDWNEVLPPWDREEDEVYPPGPYHPFPNYIRPRTLQPPSA LRRRHYHHALPPSRHYPGREAQARRAQEEAEAEERRLQEQEELENYIEHVLLRRP SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003378 |
| ORF Size | 1845 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003378.4 |
| RefSeq Size | 2545 bp |
| RefSeq ORF | 1848 bp |
| Locus ID | 7425 |
| UniProt ID | O15240 |
| Protein Families | Secreted Protein |
| MW | 67.26 kDa |
| Gene Summary | This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Neuropeptide VGF Promotes Maturation of Hippocampal Dendrites That Is Reduced by Single Nucleotide Polymorphisms
,Behnke, J;Cheedalla, A;Bhatt, V;Bhat, M;Teng, S;Palmieri, A;Windon, CC;Thakker-Varia, S;Alder, J;,
Int J Mol Sci
,PubMed ID 28287464
[VGF]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209477L1 | Lenti-ORF clone of VGF (Myc-DDK-tagged)-Human VGF nerve growth factor inducible (VGF) |
CNY 5,890.00 |
|
| RC209477L2 | Lenti-ORF clone of VGF (mGFP-tagged)-Human VGF nerve growth factor inducible (VGF) |
CNY 5,890.00 |
|
| RC209477L3 | Lenti-ORF clone of VGF (Myc-DDK-tagged)-Human VGF nerve growth factor inducible (VGF) |
CNY 5,890.00 |
|
| RC209477L4 | Lenti-ORF clone of VGF (mGFP-tagged)-Human VGF nerve growth factor inducible (VGF) |
CNY 5,890.00 |
|
| RG209477 | VGF (tGFP-tagged) - Human VGF nerve growth factor inducible (VGF) |
CNY 4,370.00 |
|
| SC322730 | VGF (untagged)-Human VGF nerve growth factor inducible (VGF) |
CNY 4,584.00 |
