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TOP1MT (NM_052963) Human Tagged ORF Clone
CAT＃: RC208637
TOP1MT (Myc-DDK-tagged)-Human topoisomerase (DNA) I, mitochondrial (TOP1MT), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_052963" in other vectors (4)
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CNY 4,472.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208637 representing NM_052963
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGTGGTGCGGCTGCTGCGGCTCCGGGCGGCTCTGACGCTGCTCGGGGAGGTCCCCCGCCGCCCGG CCTCCCGGGGTGTCCCGGGCTCGCGCAGGACGCAGAAGGGCAGTGGAGCCAGGTGGGAGAAGGAGAAGCA CGAAGACGGGGTGAAGTGGAGACAGCTGGAGCACAAGGGCCCGTACTTCGCACCCCCATACGAGCCCCTT CCCGACGGAGTGCGTTTCTTCTATGAAGGAAGGCCTGTGAGATTGAGCGTGGCAGCGGAGGAGGTCGCCA CTTTTTATGGGAGGATGTTAGATCATGAATACACAACAAAGGAGGTTTTCCGGAAGAACTTCTTCAATGA CTGGCGAAAGGAAATGGCGGTGGAAGAGAGGGAAGTCATCAAGAGCCTGGACAAGTGTGACTTCACGGAG ATCCACAGATACTTTGTGGACAAGGCCGCAGCCCGGAAAGTCCTGAGCAGGGAGGAGAAGCAGAAGCTAA AAGAAGAGGCAGAAAAACTTCAGCAAGAGTTCGGCTACTGTATTTTAGATGGTCACCAAGAAAAAATAGG CAACTTCAAGATTGAGCCGCCTGGCTTGTTCCGTGGCCGTGGCGACCATCCCAAGATGGGGATGCTGAAG AGAAGGATCACGCCAGAGGATGTGGTTATCAACTGCAGCAGGGACTCGAAGATCCCCGAGCCGCCGGCGG GGCACCAGTGGAAGGAGGTGCGCTCCGATAACACCGTCACGTGGCTGGCAGCTTGGACCGAGAGCGTTCA GAACTCCATCAAGTACATCATGCTGAACCCTTGCTCGAAGCTGAAGGGGGAGACAGCTTGGCAGAAGTTT GAAACAGCTCGACGCCTGCGGGGATTTGTGGACGAGATCCGCTCCCAGTACCGGGCTGACTGGAAGTCTC GGGAAATGAAGACGAGACAGCGGGCGGTGGCCCTGTATTTCATCGATAAGCTGGCACTGAGAGCAGGAAA TGAGAAGGAGGACGGTGAGGCGGCCGACACCGTGGGCTGCTGTTCCCTCCGCGTGGAGCACGTCCAGCTG CACCCGGAGGCCGATGGCTGCCAACACGTGGTGGAATTTGACTTCCTGGGGAAGGACTGCATCCGCTACT ACAACAGAGTGCCGGTGGAGAAGCCGGTGTACAAGAACTTACAGCTCTTTATGGAGAACAAGGACCCCCG GGACGACCTCTTCGACAGGCTGACCACGACCAGCCTGAACAAGCACCTCCAGGAGCTGATGGACGGGCTG ACGGCCAAGGTGTTCCGGACCTACAACGCCTCCATCACTCTGCAGGAGCAGCTGCGGGCCCTGACGCGCG CCGAGGACAGCATAGCAGCTAAGATCTTATCCTACAACCGAGCCAACCGAGTCGTGGCCATTCTCTGCAA CCATCAGCGAGCAACCCCCAGTACGTTCGAGAAGTCGATGCAGAATCTCCAGACGAAGATCCAGGCAAAG AAGGAGCAGGTGGCTGAGGCCAGGGCAGAGCTGAGGAGGGCGAGGGCTGAGCACAAAGCCCAAGGGGATG GCAAGTCCAGGAGTGTCCTGGAGAAGAAGAGGCGGCTCCTGGAGAAGCTGCAGGAGCAGCTGGCGCAGCT GAGTGTGCAGGCCACGGACAAGGAGGAGAACAAGCAGGTGGCCCTGGGCACGTCCAAGCTCAACTACCTG GACCCCAGGATCAGCATTGCCTGGTGCAAGCGGTTCAGGGTGCCAGTGGAGAAGATCTACAGCAAAACAC AGCGGGAGAGGTTCGCCTGGGCTCTCGCCATGGCAGGAGAAGACTTTGAATTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208637 representing NM_052963
Red=Cloning site Green=Tags(s) MRVVRLLRLRAALTLLGEVPRRPASRGVPGSRRTQKGSGARWEKEKHEDGVKWRQLEHKGPYFAPPYEPL PDGVRFFYEGRPVRLSVAAEEVATFYGRMLDHEYTTKEVFRKNFFNDWRKEMAVEEREVIKSLDKCDFTE IHRYFVDKAAARKVLSREEKQKLKEEAEKLQQEFGYCILDGHQEKIGNFKIEPPGLFRGRGDHPKMGMLK RRITPEDVVINCSRDSKIPEPPAGHQWKEVRSDNTVTWLAAWTESVQNSIKYIMLNPCSKLKGETAWQKF ETARRLRGFVDEIRSQYRADWKSREMKTRQRAVALYFIDKLALRAGNEKEDGEAADTVGCCSLRVEHVQL HPEADGCQHVVEFDFLGKDCIRYYNRVPVEKPVYKNLQLFMENKDPRDDLFDRLTTTSLNKHLQELMDGL TAKVFRTYNASITLQEQLRALTRAEDSIAAKILSYNRANRVVAILCNHQRATPSTFEKSMQNLQTKIQAK KEQVAEARAELRRARAEHKAQGDGKSRSVLEKKRRLLEKLQEQLAQLSVQATDKEENKQVALGTSKLNYL DPRISIAWCKRFRVPVEKIYSKTQRERFAWALAMAGEDFEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_052963 |
| ORF Size | 1803 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_052963.3 |
| RefSeq Size | 1953 bp |
| RefSeq ORF | 1806 bp |
| Locus ID | 116447 |
| UniProt ID | Q969P6 |
| Protein Families | Druggable Genome |
| MW | 69.9 kDa |
| Gene Summary | This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208637L3 | Lenti-ORF clone of TOP1MT (Myc-DDK-tagged)-Human topoisomerase (DNA) I, mitochondrial (TOP1MT), nuclear gene encoding mitochondrial protein |
CNY 6,872.00 |
|
| RC208637L4 | Lenti-ORF clone of TOP1MT (mGFP-tagged)-Human topoisomerase (DNA) I, mitochondrial (TOP1MT), nuclear gene encoding mitochondrial protein |
CNY 7,130.00 |
|
| RG208637 | TOP1MT (tGFP-tagged) - Human topoisomerase (DNA) I, mitochondrial (TOP1MT), nuclear gene encoding mitochondrial protein |
CNY 5,700.00 |
|
| SC126339 | TOP1MT (untagged)-Human topoisomerase (DNA) I, mitochondrial (TOP1MT), nuclear gene encoding mitochondrial protein |
CNY 2,950.00 |
