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SMC3 (NM_005445) Human Tagged ORF Clone
CAT＃: RC208401
- TrueORF®
SMC3 (Myc-DDK-tagged)-Human structural maintenance of chromosomes 3 (SMC3)
ORF Plasmid: tGFP
"NM_005445" in other vectors (4)
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CNY 8,472.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BAM; BMH; CDLS3; CSPG6; HCAP; SMC3L1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208401 representing NM_005445
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACATAAAGCAGGTGATTATCCAGGGTTTTCGAAGTTACAGAGATCAAACAATTGTAGATCCCTTCA GTTCAAAACATAATGTGATTGTGGGCAGAAATGGATCTGGAAAAAGTAACTTTTTTTATGCAATTCAGTT TGTTCTCAGTGATGAGTTTAGTCATCTTCGTCCAGAACAGCGGTTGGCTTTATTGCATGAAGGTACTGGT CCTCGTGTTATTTCTGCTTTTGTGGAGATTATTTTTGATAATTCAGACAACCGGTTACCAATCGATAAAG AGGAAGTTTCACTTCGAAGAGTTATTGGTGCCAAAAAGGATCAGTATTTCTTAGACAAGAAGATGGTCAC GAAAAATGATGTGATGAACCTCCTTGAAAGCGCTGGTTTTTCTCGAAGCAATCCTTATTATATTGTTAAA CAAGGAAAGATCAACCAGATGGCAACAGCACCAGATTCTCAGAGATTAAAGCTATTAAGAGAAGTAGCTG GTACTAGAGTGTATGACGAACGAAAGGAAGAAAGCATCTCCTTAATGAAAGAAACAGAGGGCAAACGGGA AAAAATCAATGAGTTGTTAAAATACATTGAAGAGAGATTACATACTCTAGAGGAAGAAAAGGAAGAACTA GCTCAGTATCAGAAGTGGGATAAAATGAGACGAGCCCTGGAATATACCATTTACAATCAGGAACTTAACG AGACTCGTGCCAAACTTGATGAGCTTTCTGCTAAGCGAGAGACTAGTGGAGAAAAATCCAGACAATTAAG AGATGCTCAGCAGGATGCAAGAGATAAAATGGAGGATATCGAACGCCAAGTTAGAGAATTGAAAACAAAA ATTTCAGCTATGAAAGAAGAAAAAGAACAGCTTAGTGCTGAAAGACAAGAGCAGATTAAGCAGAGGACTA AGTTGGAGCTTAAAGCCAAGGATTTACAAGATGAACTAGCAGGCAATAGTGAACAAAGGAAACGTTTATT AAAAGAGAGGCAGAAGCTGCTTGAAAAAATAGAAGAAAAGCAGAAAGAACTGGCAGAAACAGAACCCAAA TTCAACAGTGTGAAAGAGAAAGAAGAACGAGGAATTGCTAGATTGGCTCAAGCTACCCAGGAAAGAACGG ATCTTTATGCAAAGCAGGGTCGAGGAAGCCAGTTTACATCAAAAGAAGAAAGGGATAAGTGGATTAAAAA GGAACTCAAGTCTTTAGATCAGGCTATTAATGACAAGAAAAGACAGATTGCTGCTATACATAAGGATTTG GAAGACACTGAAGCAAATAAAGAGAAAAATCTGGAGCAGTATAATAAACTGGACCAGGATCTTAATGAAG TCAAAGCTCGAGTAGAAGAACTGGACAGAAAATATTACGAAGTAAAAAATAAGAAAGATGAACTACAAAG TGAAAGAAACTACTTGTGGAGAGAAGAGAATGCAGAACAGCAAGCACTTGCTGCTAAAAGAGAAGATCTT GAAAAGAAGCAACAACTTCTTAGAGCAGCAACAGGAAAGGCCATTTTAAATGGAATAGACAGCATAAACA AAGTGCTAGACCACTTCCGTCGAAAAGGAATAAACCAGCATGTTCAAAATGGCTATCATGGTATTGTAAT GAATAACTTTGAATGTGAACCAGCTTTCTACACATGCGTGGAAGTCACTGCTGGAAACAGGTTATTTTAT CACATTGTTGATTCAGATGAAGTCAGCACGAAGATTTTAATGGAGTTTAATAAAATGAATCTTCCTGGAG AGGTTACTTTTCTGCCTCTTAACAAGTTAGATGTCAGGGATACAGCCTATCCTGAAACCAATGATGCTAT TCCTATGATCAGCAAACTGAGGTACAATCCCAGATTTGACAAAGCTTTCAAACATGTGTTTGGAAAGACT CTTATTTGTCGTAGCATGGAAGTTTCAACCCAGCTGGCCCGTGCTTTCACTATGGACTGTATTACTTTGG AAGGTGACCAAGTCAGCCATCGGGGTGCTCTAACTGGGGGTTATTATGACACAAGGAAGTCTCGACTTGA ATTGCAAAAAGATGTTAGAAAAGCAGAAGAAGAACTAGGTGAACTTGAAGCAAAGCTCAATGAAAACCTG CGCAGAAATATTGAAAGGATTAATAATGAAATTGATCAGTTGATGAACCAAATGCAACAGATCGAGACCC AGCAAAGGAAATTTAAAGCATCTAGAGATAGCATATTATCAGAAATGAAGATGCTAAAAGAGAAGAGGCA GCAGTCAGAGAAAACCTTCATGCCTAAGCAACGTAGCTTACAGAGTTTGGAGGCAAGCTTGCATGCTATG GAGTCTACCAGAGAGTCATTGAAAGCAGAACTGGGAACTGATTTGCTTTCTCAACTGAGTTTGGAAGATC AGAAGAGAGTAGATGCACTGAATGATGAGATTCGTCAACTTCAGCAGGAAAACAGACAGTTGCTAAATGA AAGAATTAAATTAGAAGGTATTATTACTCGAGTAGAGACTTATCTCAATGAGAATCTGAGAAAACGCTTG GACCAAGTAGAACAGGAACTTAATGAGCTGAGAGAGACAGAAGGGGGTACTGTTCTCACAGCCACAACAT CAGAACTTGAAGCCATCAATAAAAGAGTAAAAGACACTATGGCACGATCAGAAGATTTGGACAATTCCAT TGATAAAACAGAAGCTGGAATTAAGGAGCTTCAGAAGAGTATGGAGCGCTGGAAAAATATGGAAAAAGAA CATATGGATGCTATAAATCATGATACTAAAGAACTGGAAAAGATGACAAATCGGCAAGGCATGCTATTGA AGAAGAAAGAAGAGTGTATGAAGAAAATTCGAGAACTTGGATCACTTCCCCAGGAAGCATTTGAAAAGTA CCAGACACTGAGCCTCAAACAGTTGTTTCGAAAACTTGAGCAGTGCAACACAGAATTAAAGAAGTACAGC CATGTTAACAAAAAGGCTTTGGATCAGTTTGTAAATTTCTCCGAGCAGAAAGAAAAGTTAATAAAGCGTC AAGAAGAGTTAGATAGGGGTTACAAATCAATCATGGAACTGATGAATGTACTTGAACTTCGGAAATATGA AGCTATTCAGTTAACTTTCAAACAGGTATCTAAGAACTTCAGTGAAGTATTCCAGAAGTTAGTACCTGGT GGCAAAGCTACTTTGGTGATGAAGAAAGGAGATGTGGAGGGCAGTCAGTCTCAAGATGAAGGAGAAGGGA GTGGTGAGAGTGAGAGGGGTTCTGGCTCACAAAGCAGTGTCCCATCAGTTGACCAGTTTACTGGAGTTGG AATTAGGGTGTCATTTACAGGAAAACAAGGTGAAATGAGAGAAATGCAACAGCTTTCAGGTGGACAGAAA TCCTTGGTAGCCCTTGCTCTGATTTTTGCCATTCAGAAATGTGACCCGGCTCCATTTTACTTGTTTGATG AAATTGACCAGGCTCTGGATGCTCAGCACAGAAAGGCTGTGTCAGATATGATTATGGAACTTGCTGTACA TGCTCAGTTTATTACAACTACTTTTAGGCCTGAACTGCTTGAGTCAGCTGACAAATTCTATGGTGTAAAG TTCAGAAATAAGGTTAGTCATATTGATGTGATCACAGCAGAGATGGCCAAAGACTTTGTAGAAGATGATA CCACACATGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208401 representing NM_005445
Red=Cloning site Green=Tags(s) MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNFFYAIQFVLSDEFSHLRPEQRLALLHEGTG PRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVK QGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEEL AQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTK ISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEPK FNSVKEKEERGIARLAQATQERTDLYAKQGRGSQFTSKEERDKWIKKELKSLDQAINDKKRQIAAIHKDL EDTEANKEKNLEQYNKLDQDLNEVKARVEELDRKYYEVKNKKDELQSERNYLWREENAEQQALAAKREDL EKKQQLLRAATGKAILNGIDSINKVLDHFRRKGINQHVQNGYHGIVMNNFECEPAFYTCVEVTAGNRLFY HIVDSDEVSTKILMEFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFKHVFGKT LICRSMEVSTQLARAFTMDCITLEGDQVSHRGALTGGYYDTRKSRLELQKDVRKAEEELGELEAKLNENL RRNIERINNEIDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAM ESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQENRQLLNERIKLEGIITRVETYLNENLRKRL DQVEQELNELRETEGGTVLTATTSELEAINKRVKDTMARSEDLDNSIDKTEAGIKELQKSMERWKNMEKE HMDAINHDTKELEKMTNRQGMLLKKKEECMKKIRELGSLPQEAFEKYQTLSLKQLFRKLEQCNTELKKYS HVNKKALDQFVNFSEQKEKLIKRQEELDRGYKSIMELMNVLELRKYEAIQLTFKQVSKNFSEVFQKLVPG GKATLVMKKGDVEGSQSQDEGEGSGESERGSGSQSSVPSVDQFTGVGIRVSFTGKQGEMREMQQLSGGQK SLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIMELAVHAQFITTTFRPELLESADKFYGVK FRNKVSHIDVITAEMAKDFVEDDTTHG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005445 |
| ORF Size | 3651 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005445.4 |
| RefSeq Size | 4131 bp |
| RefSeq ORF | 3654 bp |
| Locus ID | 9126 |
| UniProt ID | Q9UQE7 |
| Domains | SMC_N, SMC_C |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Oocyte meiosis |
| MW | 142 kDa |
| Gene Summary | This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208401L3 | Lenti-ORF clone of SMC3 (Myc-DDK-tagged)-Human structural maintenance of chromosomes 3 (SMC3) |
CNY 10,872.00 |
|
| RC208401L4 | Lenti-ORF clone of SMC3 (mGFP-tagged)-Human structural maintenance of chromosomes 3 (SMC3) |
CNY 11,500.00 |
|
| RG208401 | SMC3 (tGFP-tagged) - Human structural maintenance of chromosomes 3 (SMC3) |
CNY 10,072.00 |
|
| SC116740 | SMC3 (untagged)-Human structural maintenance of chromosomes 3 (SMC3) |
CNY 8,480.00 |
