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RAD21 (NM_006265) Human Tagged ORF Clone
CAT＃: RC208262
RAD21 (Myc-DDK-tagged)-Human RAD21 homolog (S. pombe) (RAD21)
ORF Plasmid: tGFP
"NM_006265" in other vectors (7)
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CNY 7,048.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDLS4; hHR21; HR21; HRAD21; MCD1; MGS; NXP1; SCC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208262 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTACGCACATTTTGTTCTCAGTAAAAGAGGGCCTCTGGCCAAAATTTGGCTAGCGGCCCATTGGG ATAAGAAGCTAACCAAAGCCCATGTGTTCGAGTGTAATTTAGAGAGCAGCGTGGAGAGTATCATCTCACC AAAGGTGAAAATGGCATTACGGACATCAGGACATCTCTTACTGGGAGTAGTTCGAATCTATCACAGGAAA GCCAAATACCTTCTTGCAGACTGTAATGAAGCATTCATTAAGATAAAGATGGCTTTTCGGCCAGGTGTGG TTGACCTGCCTGAGGAAAATCGGGAAGCAGCTTATAATGCCATTACTTTACCTGAAGAATTTCATGACTT TGATCAGCCACTGCCTGACTTAGATGACATCGATGTGGCCCAGCAGTTCAGCTTGAATCAGAGTAGAGTG GAAGAGATAACCATGAGAGAAGAAGTTGGGAACATCAGTATTTTACAAGAAAATGATTTTGGTGATTTTG GAATGGATGATCGTGAGATAATGAGAGAAGGCAGTGCTTTTGAGGATGACGACATGTTAGTAAGCACTAC TACTTCTAACCTCCTATTAGAGTCTGAACAGAGCACCAGCAATCTGAATGAGAAAATTAACCATTTAGAA TATGAAGATCAATATAAGGATGATAATTTTGGAGAAGGAAATGATGGTGGAATATTAGATGACAAACTTA TTAGTAATAATGATGGCGGTATCTTTGATGATCCCCCTGCCCTCTCTGAGGCAGGGGTGATGTTGCCAGA GCAGCCTGCACATGACGATATGGATGAGGATGATAATGTATCAATGGGTGGGCCTGATAGTCCTGATTCA GTGGATCCCGTTGAACCAATGCCAACCATGACTGATCAAACAACACTTGTTCCAAATGAGGAAGAAGCAT TTGCATTGGAGCCTATTGATATAACTGTTAAAGAAACAAAAGCCAAGAGGAAGAGGAAGCTAATTGTTGA CAGTGTCAAAGAGTTGGATAGCAAGACAATTAGAGCCCAACTTAGTGATTATTCAGATATTGTTACTACT TTGGATCTGGCACCGCCCACCAAGAAATTGATGATGTGGAAAGAGACAGGAGGAGTAGAAAAACTGTTTT CTTTACCTGCTCAGCCTTTGTGGAATAACAGACTACTGAAGCTCTTTACACGCTGTCTTACACCGCTTGT ACCAGAAGACCTTAGAAAAAGGAGGAAAGGAGGAGAGGCAGATAATTTGGATGAATTCCTCAAAGAATTT GAAAATCCAGAGGTTCCTAGAGAGGACCAGCAACAGCAGCATCAGCAGCGTGATGTTATCGATGAGCCCA TTATTGAAGAGCCAAGCCGCCTCCAGGAGTCAGTGATGGAGGCCAGCAGAACAAACATAGATGAGTCAGC TATGCCTCCACCACCACCTCAGGGAGTTAAGCGAAAAGCTGGACAAATTGACCCAGAGCCTGTGATGCCT CCTCAGCAGGTAGAGCAGATGGAAATACCACCTGTAGAGCTTCCCCCAGAAGAACCTCCAAATATCTGTC AGCTAATACCAGAGTTAGAACTTCTGCCAGAAAAAGAGAAGGAGAAAGAGAAGGAAAAAGAAGATGATGA AGAGGAAGAGGATGAAGATGCATCAGGGGGCGATCAAGATCAGGAAGAAAGAAGATGGAACAAAAGGACT CAGCAGATGCTTCATGGTCTTCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGTTTGCTTGAGT TATGTCGAAATACGAACAGAAAACAAGCTGCCGCAAAGTTCTACAGCTTCTTGGTTCTTAAAAAGCAGCA AGCTATTGAGCTGACACAGGAAGAACCGTACAGTGACATCATCGCAACACCTGGACCAAGGTTCCATATT ATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208262 protein sequence
Red=Cloning site Green=Tags(s) MFYAHFVLSKRGPLAKIWLAAHWDKKLTKAHVFECNLESSVESIISPKVKMALRTSGHLLLGVVRIYHRK AKYLLADCNEAFIKIKMAFRPGVVDLPEENREAAYNAITLPEEFHDFDQPLPDLDDIDVAQQFSLNQSRV EEITMREEVGNISILQENDFGDFGMDDREIMREGSAFEDDDMLVSTTTSNLLLESEQSTSNLNEKINHLE YEDQYKDDNFGEGNDGGILDDKLISNNDGGIFDDPPALSEAGVMLPEQPAHDDMDEDDNVSMGGPDSPDS VDPVEPMPTMTDQTTLVPNEEEAFALEPIDITVKETKAKRKRKLIVDSVKELDSKTIRAQLSDYSDIVTT LDLAPPTKKLMMWKETGGVEKLFSLPAQPLWNNRLLKLFTRCLTPLVPEDLRKRRKGGEADNLDEFLKEF ENPEVPREDQQQQHQQRDVIDEPIIEEPSRLQESVMEASRTNIDESAMPPPPPQGVKRKAGQIDPEPVMP PQQVEQMEIPPVELPPEEPPNICQLIPELELLPEKEKEKEKEKEDDEEEEDEDASGGDQDQEERRWNKRT QQMLHGLQRALAKTGAESISLLELCRNTNRKQAAAKFYSFLVLKKQQAIELTQEEPYSDIIATPGPRFHI I TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006265 |
| ORF Size | 1893 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006265.3 |
| RefSeq Size | 3773 bp |
| RefSeq ORF | 1896 bp |
| Locus ID | 5885 |
| UniProt ID | O60216 |
| Domains | Rad21_Rec8, Rad21_Rec8_N |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle |
| MW | 71.7 kDa |
| Gene Summary | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208262L1 | Lenti ORF clone of Human RAD21 homolog (S. pombe) (RAD21), Myc-DDK-tagged |
CNY 9,448.00 |
|
| RC208262L2 | Lenti ORF clone of Human RAD21 homolog (S. pombe) (RAD21), mGFP tagged |
CNY 9,448.00 |
|
| RC208262L3 | Lenti ORF clone of Human RAD21 homolog (S. pombe) (RAD21), Myc-DDK-tagged |
CNY 9,448.00 |
|
| RC208262L4 | Lenti ORF clone of Human RAD21 homolog (S. pombe) (RAD21), mGFP tagged |
CNY 9,448.00 |
|
| RG208262 | RAD21 (tGFP-tagged) - Human RAD21 homolog (S. pombe) (RAD21) |
CNY 8,648.00 |
|
| SC116225 | RAD21 (untagged)-Human RAD21 homolog (S. pombe) (RAD21) |
CNY 7,056.00 |
|
| SC322657 | RAD21 (untagged)-Human RAD21 homolog (S. pombe) (RAD21) |
CNY 7,056.00 |
