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KIF18A (NM_031217) Human Tagged ORF Clone
CAT＃: RC208235
KIF18A (Myc-DDK-tagged)-Human kinesin family member 18A (KIF18A)
ORF Plasmid: tGFP
"NM_031217" in other vectors (7)
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CNY 6,576.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MS-KIF18A; PPP1R99 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208235 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTCACTGAGGAAGACCTGTGCCACCATATGAAAGTAGTAGTTCGTGTACGTCCGGAAAACACTA AAGAAAAAGCAGCTGGATTTCATAAAGTGGTTCATGTTGTGGATAAACATATCCTAGTTTTTGATCCCAA ACAAGAAGAAGTCAGTTTTTTCCATGGAAAGAAAACTACAAATCAAAATGTTATAAAGAAACAAAATAAG GATCTTAAATTTGTATTTGATGCTGTTTTTGATGAAACGTCAACTCAGTCAGAAGTTTTTGAACACACTA CTAAGCCAATTCTTCGTAGTTTTTTGAATGGATATAATTGCACAGTACTTGCCTATGGTGCCACTGGTGC TGGGAAGACCCACACTATGCTAGGATCAGCTGATGAACCTGGAGTGATGTATCTAACAATGTTACACCTT TACAAATGCATGGATGAGATTAAAGAAGAGAAAATATGTAGTACTGCAGTTTCATATCTGGAGGTATATA ATGAACAGATTCGTGATCTCTTAGTAAATTCAGGGCCACTTGCTGTCCGGGAAGATACCCAAAAAGGGGT GGTCGTTCATGGACTTACTTTACACCAGCCCAAATCCTCAGAAGAAATTTTACATTTATTGGATAATGGA AACAAAAACAGGACACAACATCCCACTGATATGAATGCCACATCTTCTCGTTCTCATGCTGTTTTCCAAA TTTACTTGCGACAACAAGACAAAACAGCAAGTATCAATCAAAATGTCCGTATTGCCAAGATGTCACTCAT TGACCTGGCAGGATCTGAGCGAGCAAGTACTTCCGGTGCTAAGGGGACCCGATTTGTAGAAGGCACAAAT ATTAATAGATCACTTTTAGCTCTTGGGAATGTCATCAATGCCTTAGCAGATTCAAAGAGAAAGAATCAGC ATATCCCTTACAGAAATAGTAAGCTTACTCGCTTGTTAAAGGATTCTCTTGGAGGAAACTGTCAAACTAT AATGATAGCTGCTGTTAGTCCTTCCTCTGTATTCTACGATGACACATATAACACTCTTAAGTATGCTAAC CGGGCAAAGGACATTAAATCTTCTTTGAAGAGCAATGTTCTTAATGTCAATAATCATATAACTCAATATG TAAAGATCTGTAATGAGCAGAAGGCAGAGATTTTATTGTTAAAAGAAAAACTAAAAGCCTATGAAGAACA GAAAGCCTTCACTAATGAAAATGACCAAGCAAAGTTAATGATTTCAAACCCTCAGGAAAAAGAAATCGAA AGGTTTCAAGAAATCCTGAACTGCTTGTTCCAGAATCGAGAAGAAATTAGACAAGAATATCTGAAGTTGG AAATGTTACTTAAAGAAAATGAACTTAAATCATTCTACCAACAACAGTGCCATAAACAAATAGAAATGAT GTGTTCTGAAGACAAAGTAGAAAAGGCCACTGGAAAACGAGATCATAGACTTGCAATGTTGAAAACTCGT CGCTCCTACCTGGAGAAAAGGAGGGAGGAGGAATTGAAGCAATTTGATGAGAATACTAATTGGCTCCATC GTGTCGAAAAAGAAATGGGACTCTTAAGTCAAAACGGTCATATTCCAAAGGAACTCAAGAAAGATCTTCA TTGTCACCATTTGCACCTCCAGAACAAAGATTTGAAAGCACAAATTAGACATATGATGGATCTAGCTTGT CTTCAGGAACAGCAACACAGGCAGACTGAAGCAGTATTGAATGCTTTACTTCCAACCCTAAGAAAACAAT ATTGCACATTAAAAGAAGCCGGCCTGTCAAATGCTGCTTTTGAATCTGACTTCAAAGAGATCGAACATTT GGTAGAGAGGAAAAAAGTGGTAGTTTGGGCTGACCAAACTGCCGAACAACCAAAGCAAAACGATCTACCA GGGATTTCTGTTCTTATGACCTTTCCACAACTTGGACCAGTTCAGCCTATTCCTTGTTGCTCATCTTCAG GTGGAACTAATCTGGTTAAGATTCCTACAGAAAAAAGAACTCGGAGAAAACTAATGCCATCTCCCTTGAA AGGACAGCATACTCTAAAGTCTCCACCATCTCAAAGTGTGCAGCTCAATGATTCTCTTAGCAAAGAACTT CAGCCTATTGTATATACACCAGAAGACTGTAGAAAAGCTTTTCAAAATCCGTCTACAGTAACCTTAATGA AACCATCATCATTTACTACAAGTTTTCAGGCTGTCAGCTCAAACATAAACAGTGATAATTGTCTGAAAAT GTTGTGTGAAGTAGCTATCCCTCATAATAGAAGAAAAGAATGTGGACAGGAGGACTTGGACTCTACATTT ACTATATGTGAAGACATCAAGAGCTCGAAGTGTAAATTACCCGAACAAGAATCACTACCAAATGATAACA AAGACATTTTACAACGGCTTGATCCTTCTTCATTCTCAACTAAGCATTCTATGCCTGTACCAAGCATGGT GCCATCCTACATGGCAATGACTACTGCTGCCAAAAGGAAACGGAAATTAACAAGTTCTACATCAAACAGT TCGTTAACTGCAGACGTAAATTCTGGATTTGCCAAACGTGTTCGACAAGATAATTCAAGTGAGAAGCACT TACAAGAAAACAAACCAACAATGGAACATAAAAGAAACATCTGTAAAATAAATCCAAGCATGGTTAGAAA ATTTGGAAGAAATATTTCAAAAGGAAATCTAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208235 protein sequence
Red=Cloning site Green=Tags(s) MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTTNQNVIKKQNK DLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKTHTMLGSADEPGVMYLTMLHL YKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVREDTQKGVVVHGLTLHQPKSSEEILHLLDNG NKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGTN INRSLLALGNVINALADSKRKNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYAN RAKDIKSSLKSNVLNVNNHITQYVKICNEQKAEILLLKEKLKAYEEQKAFTNENDQAKLMISNPQEKEIE RFQEILNCLFQNREEIRQEYLKLEMLLKENELKSFYQQQCHKQIEMMCSEDKVEKATGKRDHRLAMLKTR RSYLEKRREEELKQFDENTNWLHRVEKEMGLLSQNGHIPKELKKDLHCHHLHLQNKDLKAQIRHMMDLAC LQEQQHRQTEAVLNALLPTLRKQYCTLKEAGLSNAAFESDFKEIEHLVERKKVVVWADQTAEQPKQNDLP GISVLMTFPQLGPVQPIPCCSSSGGTNLVKIPTEKRTRRKLMPSPLKGQHTLKSPPSQSVQLNDSLSKEL QPIVYTPEDCRKAFQNPSTVTLMKPSSFTTSFQAVSSNINSDNCLKMLCEVAIPHNRRKECGQEDLDSTF TICEDIKSSKCKLPEQESLPNDNKDILQRLDPSSFSTKHSMPVPSMVPSYMAMTTAAKRKRKLTSSTSNS SLTADVNSGFAKRVRQDNSSEKHLQENKPTMEHKRNICKINPSMVRKFGRNISKGNLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031217 |
| ORF Size | 2694 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031217.4 |
| RefSeq Size | 3463 bp |
| RefSeq ORF | 2697 bp |
| Locus ID | 81930 |
| UniProt ID | Q8NI77 |
| Domains | kinesin |
| Protein Families | Druggable Genome |
| MW | 102.3 kDa |
| Gene Summary | KIF18A is a member of the kinesin superfamily of microtubule-associated molecular motors (see MIM 148760) that use hydrolysis of ATP to produce force and movement along microtubules (Luboshits and Benayahu, 2005 [PubMed 15878648]).[supplied by OMIM, Aug 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208235L1 | Lenti ORF clone of Human kinesin family member 18A (KIF18A), Myc-DDK-tagged |
CNY 8,976.00 |
|
| RC208235L2 | Lenti ORF clone of Human kinesin family member 18A (KIF18A), mGFP tagged |
CNY 9,030.00 |
|
| RC208235L3 | Lenti ORF clone of Human kinesin family member 18A (KIF18A), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC208235L4 | Lenti ORF clone of Human kinesin family member 18A (KIF18A), mGFP tagged |
CNY 8,976.00 |
|
| RG208235 | KIF18A (tGFP-tagged) - Human kinesin family member 18A (KIF18A) |
CNY 7,890.00 |
|
| SC108713 | KIF18A (untagged)-Human kinesin family member 18A (KIF18A) |
CNY 2,950.00 |
|
| SC324239 | KIF18A (untagged)-Human kinesin family member 18A (KIF18A) |
CNY 2,950.00 |
