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P2X1 (P2RX1) (NM_002558) Human Tagged ORF Clone
CAT＃: RC207924
- TrueORF®
P2RX1 (Myc-DDK-tagged)-Human purinergic receptor P2X, ligand-gated ion channel, 1 (P2RX1)
ORF Plasmid: tGFP
"NM_002558" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | P2X1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207924 representing NM_002558
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACGGCGGTTCCAGGAGGAGCTGGCCGCCTTCCTCTTCGAGTATGACACCCCCCGCATGGTGCTGG TGCGTAATAAGAAGGTGGGCGTTATCTTCCGACTGATCCAGCTGGTGGTCCTGGTCTACGTCATCGGGTG GGTGTTTCTCTATGAGAAGGGCTACCAGACCTCGAGCGGCCTCATCAGCAGTGTCTCTGTGAAACTCAAG GGCCTGGCCGTGACCCAGCTCCCTGGCCTCGGCCCCCAGGTCTGGGATGTGGCTGACTACGTCTTCCCAG CCCAGGGGGACAACTCCTTCGTGGTCATGACCAATTTCATCGTGACCCCGAAGCAGACTCAAGGCTACTG CGCAGAGCACCCAGAAGGGGGCATATGCAAGGAAGACAGTGGCTGTACCCCTGGGAAGGCCAAGAGGAAG GCCCAAGGCATCCGCACGGGCAAGTGTGTGGCCTTCAACGACACTGTGAAGACGTGTGAGATCTTTGGCT GGTGCCCCGTGGAGGTGGATGACGACATCCCGCGCCCTGCCCTTCTCCGAGAGGCCGAGAACTTCACTCT TTTCATCAAGAACAGCATCAGCTTTCCACGCTTCAAGGTCAACAGGCGCAACCTGGTGGAGGAGGTGAAT GCTGCCCACATGAAGACCTGCCTCTTTCACAAGACCCTGCACCCCCTGTGCCCAGTCTTCCAGCTTGGCT ACGTGGTGCAAGAGTCAGGCCAGAACTTCAGCACCCTGGCTGAGAAGGGTGGAGTGGTTGGCATCACCAT CGACTGGCACTGTGACCTGGACTGGCACGTACGGCACTGCAGACCCATCTATGAGTTCCATGGGCTGTAC GAAGAGAAAAATCTCTCCCCAGGCTTCAACTTCAGGTTTGCCAGGCACTTTGTGGAGAACGGGACCAACT ACCGTCACCTCTTCAAGGTGTTTGGGATTCGCTTTGACATCCTGGTGGACGGCAAGGCCGGGAAGTTTGA CATCATCCCTACAATGACCACCATCGGCTCTGGAATTGGCATCTTTGGGGTGGCCACAGTTCTCTGTGAC CTGCTGCTGCTTCACATCCTGCCTAAGAGGCACTACTACAAGCAGAAGAAGTTCAAATACGCTGAGGACA TGGGGCCAGGGGCGGCTGAGCGTGACCTCGCAGCTACCAGCTCCACCCTGGGCCTGCAGGAGAACATGAG GACATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207924 representing NM_002558
Red=Cloning site Green=Tags(s) MARRFQEELAAFLFEYDTPRMVLVRNKKVGVIFRLIQLVVLVYVIGWVFLYEKGYQTSSGLISSVSVKLK GLAVTQLPGLGPQVWDVADYVFPAQGDNSFVVMTNFIVTPKQTQGYCAEHPEGGICKEDSGCTPGKAKRK AQGIRTGKCVAFNDTVKTCEIFGWCPVEVDDDIPRPALLREAENFTLFIKNSISFPRFKVNRRNLVEEVN AAHMKTCLFHKTLHPLCPVFQLGYVVQESGQNFSTLAEKGGVVGITIDWHCDLDWHVRHCRPIYEFHGLY EEKNLSPGFNFRFARHFVENGTNYRHLFKVFGIRFDILVDGKAGKFDIIPTMTTIGSGIGIFGVATVLCD LLLLHILPKRHYYKQKKFKYAEDMGPGAAERDLAATSSTLGLQENMRTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002558 |
| ORF Size | 1197 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002558.4 |
| RefSeq Size | 2885 bp |
| RefSeq ORF | 1200 bp |
| Locus ID | 5023 |
| UniProt ID | P51575 |
| Domains | P2X_receptor |
| Protein Families | Druggable Genome, Ion Channels: ATP Receptors, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Neuroactive ligand-receptor interaction |
| MW | 45.4 kDa |
| Gene Summary | The protein encoded by this gene belongs to the P2X family of G-protein-coupled receptors. These proteins can form homo-and heterotimers and function as ATP-gated ion channels and mediate rapid and selective permeability to cations. This protein is primarily localized to smooth muscle where binds ATP and mediates synaptic transmission between neurons and from neurons to smooth muscle and may being responsible for sympathetic vasoconstriction in small arteries, arterioles and vas deferens. Mouse studies suggest that this receptor is essential for normal male reproductive function. This protein may also be involved in promoting apoptosis. [provided by RefSeq, Jun 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Tonic Calcium-Activated Chloride Current Sustained by ATP Release and Highly Desensitizing Human P2X1 Receptors
,Limon, A;Hagopian, G;Reyes-Ruiz, JM;Miledi, R;,
Neuroscience
,PubMed ID 31349005
[P2RX1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207924L3 | Lenti-ORF clone of P2RX1 (Myc-DDK-tagged)-Human purinergic receptor P2X, ligand-gated ion channel, 1 (P2RX1) |
CNY 5,890.00 |
|
| RC207924L4 | Lenti-ORF clone of P2RX1 (mGFP-tagged)-Human purinergic receptor P2X, ligand-gated ion channel, 1 (P2RX1) |
CNY 5,890.00 |
|
| RG207924 | P2RX1 (tGFP-tagged) - Human purinergic receptor P2X, ligand-gated ion channel, 1 (P2RX1) |
CNY 4,370.00 |
|
| SC118594 | P2RX1 (untagged)-Human purinergic receptor P2X, ligand-gated ion channel, 1 (P2RX1) |
CNY 2,950.00 |
