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YTHDF1 (NM_017798) Human Tagged ORF Clone
CAT＃: RC207185
YTHDF1 (Myc-DDK-tagged)-Human YTH domain family, member 1 (YTHDF1)
ORF Plasmid: tGFP
"NM_017798" in other vectors (6)
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CNY 3,990.00
| Cited in 5 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C20orf21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207185 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCACCAGCGTGGACACCCAGAGAACAAAAGGACAAGATAATAAAGTACAAAATGGTTCGTTAC ATCAGAAGGATACAGTTCATGACAATGACTTTGAGCCCTACCTTACTGGACAGTCAAATCAGAGTAACAG TTACCCCTCAATGAGCGACCCCTACCTGTCCAGCTATTACCCGCCGTCCATTGGATTTCCTTACTCCCTC AATGAGGCTCCGTGGTCTACTGCAGGGGACCCTCCGATTCCATACCTCACCACCTACGGACAGCTCAGTA ACGGAGACCATCATTTTATGCACGATGCTGTTTTTGGGCAGCCTGGGGGCCTGGGGAACAACATCTATCA GCACAGGTTCAATTTTTTCCCTGAAAACCCTGCGTTCTCAGCATGGGGGACAAGTGGGTCTCAAGGTCAG CAGACCCAGAGCTCCGCGTATGGGAGCAGCTACACCTACCCCCCGAGCTCCCTGGGTGGCACGGTGGTTG ATGGGCAGCCAGGCTTTCACAGCGACACCCTCAGCAAGGCCCCCGGGATGAACAGCCTGGAGCAGGGCAT GGTTGGCCTGAAGATTGGGGACGTCAGCTCCTCCGCCGTCAAGACGGTGGGCTCTGTCGTCAGCAGCGTG GCACTGACTGGTGTCCTTTCTGGCAACGGTGGGACAAATGTGAACATGCCAGTTTCAAAGCCGACCTCGT GGGCTGCCATTGCCAGCAAGCCTGCAAAACCACAGCCTAAAATGAAAACAAAGAGCGGGCCTGTCATGGG GGGTGGGCTGCCCCCTCCACCCATAAAGCATAACATGGACATTGGCACCTGGGATAACAAGGGGCCTGTG CCGAAGGCCCCAGTCCCCCAGCAGGCACCCTCTCCACAGGCTGCCCCACAGCCCCAGCAGGTGGCTCAGC CTCTCCCAGCACAGCCCCCAGCTTTGGCTCAACCGCAGTATCAGAGCCCTCAGCAGCCACCCCAGACCCG CTGGGTTGCCCCACGCAACAGAAACGCGGCGTTTGGGCAGAGCGGAGGGGCTGGCAGCGATAGCAACTCT CCTGGAAACGTCCAGCCTAATTCTGCCCCCAGCGTCGAATCCCACCCCGTCCTTGAAAAACTGAAGGCTG CTCACAGCTACAACCCGAAAGAGTTTGAGTGGAATCTGAAAAGCGGGCGTGTGTTCATCATCAAGAGCTA CTCTGAGGACGACATCCACCGCTCCATTAAGTACTCCATCTGGTGTAGCACAGAGCACGGCAACAAGCGC CTGGACAGCGCCTTCCGCTGCATGAGCAGCAAGGGGCCCGTCTACCTGCTCTTCAGCGTCAATGGGAGTG GGCATTTTTGTGGGGTGGCCGAGATGAAGTCCCCCGTGGACTACGGCACCAGTGCCGGGGTCTGGTCTCA GGACAAGTGGAAGGGGAAGTTTGATGTCCAGTGGATTTTTGTTAAGGATGTACCCAATAACCAGCTCCGG CACATCAGGCTGGAGAATAACGACAACAAACCGGTCACAAACTCCCGGGACACCCAGGAGGTGCCCTTAG AAAAAGCCAAGCAAGTGCTGAAAATTATCAGTTCCTACAAGCACACAACCTCCATCTTCGACGACTTTGC TCACTACGAGAAGCGCCAGGAGGAGGAGGAGGTGGTGCGCAAGGAACGGCAGAGTCGAAACAAACAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207185 protein sequence
Red=Cloning site Green=Tags(s) MSATSVDTQRTKGQDNKVQNGSLHQKDTVHDNDFEPYLTGQSNQSNSYPSMSDPYLSSYYPPSIGFPYSL NEAPWSTAGDPPIPYLTTYGQLSNGDHHFMHDAVFGQPGGLGNNIYQHRFNFFPENPAFSAWGTSGSQGQ QTQSSAYGSSYTYPPSSLGGTVVDGQPGFHSDTLSKAPGMNSLEQGMVGLKIGDVSSSAVKTVGSVVSSV ALTGVLSGNGGTNVNMPVSKPTSWAAIASKPAKPQPKMKTKSGPVMGGGLPPPPIKHNMDIGTWDNKGPV PKAPVPQQAPSPQAAPQPQQVAQPLPAQPPALAQPQYQSPQQPPQTRWVAPRNRNAAFGQSGGAGSDSNS PGNVQPNSAPSVESHPVLEKLKAAHSYNPKEFEWNLKSGRVFIIKSYSEDDIHRSIKYSIWCSTEHGNKR LDSAFRCMSSKGPVYLLFSVNGSGHFCGVAEMKSPVDYGTSAGVWSQDKWKGKFDVQWIFVKDVPNNQLR HIRLENNDNKPVTNSRDTQEVPLEKAKQVLKIISSYKHTTSIFDDFAHYEKRQEEEEVVRKERQSRNKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017798 |
| ORF Size | 1677 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017798.4 |
| RefSeq Size | 3277 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 54915 |
| UniProt ID | Q9BYJ9 |
| MW | 60.9 kDa |
| Gene Summary | Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency (PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (PubMed:26046440). Required to facilitate learning and memory formation in the hippocampus by enhancing protein synthesis upon neuronal stimulation: in response to neuronal stimulation, binds to m6A-containing neuronal mRNAs, promoting their translation, thereby contributing to learning and memory (By similarity). Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts, thereby promoting their translation (By similarity). Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (By similarity). Acts by binding and promoting translation of m6A-containing transcripts encoding proteins involved in lysosomal degradation and phagosome maturation, leading to increased antigen degradation in myeloid dendritic cells (By similarity). In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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YTHDF1 Aggravates the Progression of Cervical Cancer Through m6A-Mediated Up-Regulation of RANBP2
,Wang, H;Luo, Q;Kang, J;Wei, Q;Yang, Y;Yang, D;Liu, X;Liu, T;Yi, P;,
Frontiers in Oncology
,PubMed ID 33816306
[YTHDF1]
|
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Targeting the m6A-reader Protein YTHDF1 Effectively Re-Sensitizes Cisplatin Resistant Colon Cancer Cells through Modulating the Glutaminase (GLS)-Mediated Glutamine Metabolism
,Chen, P;Liu, X;Lin, X;Gao, L;Zhang, S;Huang, X;,
Molecular Therapy - Oncolytics
,PubMed ID 33614908
[YTHDF1]
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microRNA-421-3p prevents inflammatory response in cerebral ischemia/reperfusion injury through targeting m6A Reader YTHDF1 to inhibit p65 mRNA translation
,Zheng, L;Tang, X;Lu, M;Sun, S;Xie, S;Cai, J;Zan, J;,
International Immunopharmacology
,PubMed ID 32890792
[YTHDF1]
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The m6A reader YTHDF1 promotes ovarian cancer progression via augmenting EIF3C translation
,Liu, T;Wei, Q;Jin, J;Luo, Q;Liu, Y;Yang, Y;Cheng, C;Li, L;Pi, J;Si, Y;Xiao, H;Li, L;Rao, S;Wang, F;Yu, J;Yu, J;Zou, D;Yi, P;,
Nucleic Acids Res.
,PubMed ID 31996915
[YTHDF1]
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Cap-independent mRNA translation is upregulated in long-lived endocrine mutant mice
,Ozkurede, U;Kala, R;Johnson, C;Shen, Z;Miller, RA;Garcia, GG;,
J. Mol. Endocrinol.
,PubMed ID 31357177
[YTHDF1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207185L1 | Lenti ORF clone of Human YTH domain family, member 1 (YTHDF1), Myc-DDK-tagged |
CNY 6,560.00 |
|
| RC207185L2 | Lenti ORF clone of Human YTH domain family, member 1 (YTHDF1), mGFP tagged |
CNY 5,890.00 |
|
| RC207185L3 | Lenti ORF clone of Human YTH domain family, member 1 (YTHDF1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207185L4 | Lenti ORF clone of Human YTH domain family, member 1 (YTHDF1), mGFP tagged |
CNY 5,890.00 |
|
| RG207185 | YTHDF1 (tGFP-tagged) - Human YTH domain family, member 1 (YTHDF1) |
CNY 4,370.00 |
|
| SC113954 | YTHDF1 (untagged)-Human YTH domain family, member 1 (YTHDF1) |
CNY 4,168.00 |
