CYTL1 (NM_018659) Human Tagged ORF Clone

Name: CYTL1 (NM_018659) Human Tagged ORF Clone
SKU: RC206778
Price: 1200.00

CAT＃: RC206778

CYTL1 (Myc-DDK-tagged)-Human cytokine-like 1 (CYTL1)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_018659" in other vectors (6)

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CNY 1,200.00

CNY 3,705.00

货期*

现货

规格

Cited in 1 publication.

Product images

经常一起买 (5)

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pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

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PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA315520

Rabbit polyclonal anti-CYTL1 antibody

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C4orf4; C17
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC206778 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACGCCTGGGCCTCTGCCCGTGCTGCTGCTGCTCCTGGCGGGAGCCCCCGCCGCGCGGCCCACTC CCCCGACCTGCTACTCCCGCATGCGGGCCCTGAGCCAGGAGATCACCCGCGACTTCAACCTCCTGCAGGT CTCGGAGCCCTCGGAGCCATGTGTGAGATACCTGCCCAGGCTGTACCTGGACATACACAATTACTGTGTG CTGGACAAGCTGCGGGACTTTGTGGCCTCGCCCCCGTGTTGGAAAGTGGCCCAGGTAGATTCCTTGAAGG ACAAAGCACGGAAGCTGTACACCATCATGAACTCGTTCTGCAGGAGAGATTTGGTATTCCTGTTGGATGA CTGCAATGCCTTGGAATACCCAATCCCAGTGACTACGGTCCTGCCAGATCGTCAGCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC206778 protein sequence Red=Cloning site Green=Tags(s) MRTPGPLPVLLLLLAGAPAARPTPPTCYSRMRALSQEITRDFNLLQVSEPSEPCVRYLPRLYLDIHNYCV LDKLRDFVASPPCWKVAQVDSLKDKARKLYTIMNSFCRRDLVFLLDDCNALEYPIPVTTVLPDRQR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_018659
ORF Size	408 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_018659.3
RefSeq Size	1019 bp
RefSeq ORF	411 bp
Locus ID	54360
UniProt ID	Q9NRR1
Protein Families	Druggable Genome, Secreted Protein
MW	15.6 kDa
Gene Summary	C17 is a cytokine-like protein specifically expressed in bone marrow and cord blood mononuclear cells that bear the CD34 (MIM 142230) surface marker (Liu et al., 2000 [PubMed 10857752]).[supplied by OMIM, Mar 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Intracellular CYTL1, a novel tumor suppressor, stabilizes NDUFV1 to inhibit metabolic reprogramming in breast cancer ,null, Signal Transduction and Targeted Therapy ,PubMed ID 35115484 [CYTL1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC206778L1	Lenti ORF clone of Human cytokine-like 1 (CYTL1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,600.00 CNY 5,890.00
RC206778L2	Lenti ORF clone of Human cytokine-like 1 (CYTL1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC206778L3	Lenti ORF clone of Human cytokine-like 1 (CYTL1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,600.00 CNY 5,890.00
RC206778L4	Lenti ORF clone of Human cytokine-like 1 (CYTL1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,600.00 CNY 5,890.00
RG206778	CYTL1 (tGFP-tagged) - Human cytokine-like 1 (CYTL1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,800.00 CNY 4,370.00
SC122877	CYTL1 (untagged)-Human cytokine-like 1 (CYTL1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 2,950.00