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CARD12 (NLRC4) (NM_021209) Human Tagged ORF Clone
CAT＃: RC206757
NLRC4 (Myc-DDK-tagged)-Human NLR family, CARD domain containing 4 (NLRC4), transcript variant 1
ORF Plasmid: tGFP
"NM_021209" in other vectors (6)
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CNY 7,128.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206757 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTTCATAAAGGACAATAGCCGAGCCCTTATTCAAAGAATGGGAATGACTGTTATAAAGCAAATCA CAGATGACCTATTTGTATGGAATGTTCTGAATCGCGAAGAAGTAAACATCATTTGCTGCGAGAAGGTGGA GCAGGATGCTGCTAGAGGGATCATTCACATGATTTTGAAAAAGGGTTCAGAGTCCTGTAACCTCTTTCTT AAATCCCTTAAGGAGTGGAACTATCCTCTATTTCAGGACTTGAATGGACAAAGTCTTTTTCATCAGACAT CAGAAGGAGACTTGGACGATTTGGCTCAGGATTTAAAGGACTTGTACCATACCCCATCTTTTCTGAACTT TTATCCCCTTGGTGAAGATATTGACATTATTTTTAACTTGAAAAGCACCTTCACAGAACCTGTCCTGTGG AGGAAGGACCAACACCATCACCGCGTGGAGCAGCTGACCCTGAATGGCCTCCTGCAGGCTCTTCAGAGCC CCTGCATCATTGAAGGGGAATCTGGCAAAGGCAAGTCCACTCTGCTGCAGCGAATTGCCATGCTCTGGGG CTCCGGAAAGTGCAAGGCTCTGACCAAGTTCAAATTCGTCTTCTTCCTCCGTCTCAGCAGGGCCCAGGGT GGACTTTTTGAAACCCTCTGTGATCAACTCCTGGATATACCTGGCACAATCAGGAAGCAGACATTCATGG CCATGCTGCTGAAGCTGCGGCAGAGGGTTCTTTTCCTTCTTGATGGCTACAATGAATTCAAGCCCCAGAA CTGCCCAGAAATCGAAGCCCTGATAAAGGAAAACCACCGCTTCAAGAACATGGTCATCGTCACCACTACC ACTGAGTGCCTGAGGCACATACGGCAGTTTGGTGCCCTGACTGCTGAGGTGGGGGATATGACAGAAGACA GCGCCCAGGCTCTCATCCGAGAAGTGCTGATCAAGGAGCTTGCTGAAGGCTTGTTGCTCCAAATTCAGAA ATCCAGGTGCTTGAGGAATCTCATGAAGACCCCTCTCTTTGTGGTCATCACTTGTGCAATCCAGATGGGT GAAAGTGAGTTCCACTCTCACACACAAACAACGCTGTTCCATACCTTCTATGATCTGTTGATACAGAAAA ACAAACACAAACATAAAGGTGTGGCTGCAAGTGACTTCATTCGGAGCCTGGACCACTGTGGAGACCTAGC TCTGGAGGGTGTGTTCTCCCACAAGTTTGATTTCGAACTGCAGGATGTGTCCAGCGTGAATGAGGATGTC CTGCTGACAACTGGGCTCCTCTGTAAATATACAGCTCAAAGGTTCAAGCCAAAGTATAAATTCTTTCACA AGTCATTCCAGGAGTACACAGCAGGACGAAGACTCAGCAGTTTATTGACGTCTCATGAGCCAGAGGAGGT GACCAAGGGGAATGGTTACTTGCAGAAAATGGTTTCCATTTCGGACATTACATCCACTTATAGCAGCCTG CTCCGGTACACCTGTGGGTCATCTGTGGAAGCCACCAGGGCTGTTATGAAGCACCTCGCAGCAGTGTATC AACACGGCTGCCTTCTCGGACTTTCCATCGCCAAGAGGCCTCTCTGGAGACAGGAATCTTTGCAAAGTGT GAAAAACACCACTGAGCAAGAAATTCTGAAAGCCATAAACATCAATTCCTTTGTAGAGTGTGGCATCCAT TTATATCAAGAGAGTACATCCAAATCAGCCCTGAGCCAAGAATTTGAAGCTTTCTTTCAAGGTAAAAGCT TATATATCAACTCAGGGAACATCCCCGATTACTTATTTGACTTCTTTGAACATTTGCCCAATTGTGCAAG TGCCCTGGACTTCATTAAACTGGACTTTTATGGGGGAGCTATGGCTTCATGGGAAAAGGCTGCAGAAGAC ACAGGTGGAATCCACATGGAAGAGGCCCCAGAAACCTACATTCCCAGCAGGGCTGTATCTTTGTTCTTCA ACTGGAAGCAGGAATTCAGGACTCTGGAGGTCACACTCCGGGATTTCAGCAAGTTGAATAAGCAAGATAT CAGATATCTGGGGAAAATATTCAGCTCTGCCACAAGCCTCAGGCTGCAAATAAAGAGATGTGCTGGTGTG GCTGGAAGCCTCAGTTTGGTCCTCAGCACCTGTAAGAACATTTATTCTCTCATGGTGGAAGCCAGTCCCC TCACCATAGAAGATGAGAGGCACATCACATCTGTAACAAACCTGAAAACCTTGAGTATTCATGACCTACA GAATCAACGGCTGCCGGGTGGTCTGACTGACAGCTTGGGTAACTTGAAGAACCTTACAAAGCTCATAATG GATAACATAAAGATGAATGAAGAAGATGCTATAAAACTAGCTGAAGGCCTGAAAAACCTGAAGAAGATGT GTTTATTTCATTTGACCCACTTGTCTGACATTGGAGAGGGAATGGATTACATAGTCAAGTCTCTGTCAAG TGAACCCTGTGACCTTGAAGAAATTCAATTAGTCTCCTGCTGCTTGTCTGCAAATGCAGTGAAAATCCTA GCTCAGAATCTTCACAATTTGGTCAAACTGAGCATTCTTGATTTATCAGAAAATTACCTGGAAAAAGATG GAAATGAAGCTCTTCATGAACTGATCGACAGGATGAACGTGCTAGAACAGCTCACCGCACTGATGCTGCC CTGGGGCTGTGACGTGCAAGGCAGCCTGAGCAGCCTGTTGAAACATTTGGAGGAGGTCCCACAACTCGTC AAGCTTGGGTTGAAAAACTGGAGACTCACAGATACAGAGATTAGAATTTTAGGTGCATTTTTTGGAAAGA ACCCTCTGAAAAACTTCCAGCAGTTGAATTTGGCGGGAAATCGTGTGAGCAGTGATGGATGGCTTGCCTT CATGGGTGTATTTGAGAATCTTAAGCAATTAGTGTTTTTTGACTTTAGTACTAAAGAATTTCTACCTGAT CCAGCATTAGTCAGAAAACTTAGCCAAGTGTTATCCAAGTTAACTTTTCTGCAAGAAGCTAGGCTTGTTG GGTGGCAATTTGATGATGATGATCTCAGTGTTATTACAGGTGCTTTTAAACTAGTAACTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206757 protein sequence
Red=Cloning site Green=Tags(s) MNFIKDNSRALIQRMGMTVIKQITDDLFVWNVLNREEVNIICCEKVEQDAARGIIHMILKKGSESCNLFL KSLKEWNYPLFQDLNGQSLFHQTSEGDLDDLAQDLKDLYHTPSFLNFYPLGEDIDIIFNLKSTFTEPVLW RKDQHHHRVEQLTLNGLLQALQSPCIIEGESGKGKSTLLQRIAMLWGSGKCKALTKFKFVFFLRLSRAQG GLFETLCDQLLDIPGTIRKQTFMAMLLKLRQRVLFLLDGYNEFKPQNCPEIEALIKENHRFKNMVIVTTT TECLRHIRQFGALTAEVGDMTEDSAQALIREVLIKELAEGLLLQIQKSRCLRNLMKTPLFVVITCAIQMG ESEFHSHTQTTLFHTFYDLLIQKNKHKHKGVAASDFIRSLDHCGDLALEGVFSHKFDFELQDVSSVNEDV LLTTGLLCKYTAQRFKPKYKFFHKSFQEYTAGRRLSSLLTSHEPEEVTKGNGYLQKMVSISDITSTYSSL LRYTCGSSVEATRAVMKHLAAVYQHGCLLGLSIAKRPLWRQESLQSVKNTTEQEILKAININSFVECGIH LYQESTSKSALSQEFEAFFQGKSLYINSGNIPDYLFDFFEHLPNCASALDFIKLDFYGGAMASWEKAAED TGGIHMEEAPETYIPSRAVSLFFNWKQEFRTLEVTLRDFSKLNKQDIRYLGKIFSSATSLRLQIKRCAGV AGSLSLVLSTCKNIYSLMVEASPLTIEDERHITSVTNLKTLSIHDLQNQRLPGGLTDSLGNLKNLTKLIM DNIKMNEEDAIKLAEGLKNLKKMCLFHLTHLSDIGEGMDYIVKSLSSEPCDLEEIQLVSCCLSANAVKIL AQNLHNLVKLSILDLSENYLEKDGNEALHELIDRMNVLEQLTALMLPWGCDVQGSLSSLLKHLEEVPQLV KLGLKNWRLTDTEIRILGAFFGKNPLKNFQQLNLAGNRVSSDGWLAFMGVFENLKQLVFFDFSTKEFLPD PALVRKLSQVLSKLTFLQEARLVGWQFDDDDLSVITGAFKLVTA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021209 |
| ORF Size | 3072 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021209.4 |
| RefSeq Size | 3385 bp |
| RefSeq ORF | 3075 bp |
| Locus ID | 58484 |
| UniProt ID | Q9NPP4 |
| Domains | LRR |
| Protein Pathways | NOD-like receptor signaling pathway |
| MW | 116.2 kDa |
| Gene Summary | This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Caspase-1 interdomain linker cleavage is required for pyroptosis
,Ball, DP;Taabazuing, CY;Griswold, AR;Orth, EL;Rao, SD;Kotliar, IB;Vostal, LE;Johnson, DC;Bachovchin, DA;,
Life Sci Alliance
,PubMed ID 32051255
[CARD12]
|
|
Mitochondrial Ca(2+)-dependent NLRP3 activation exacerbates the Pseudomonas aeruginosa-driven inflammatory response in cystic fibrosis
,Rimessi, A;Bezzerri, V;Patergnani, S;Marchi, S;Cabrini, G;Pinton, P;,
Nat Commun
,PubMed ID 25648527
[CARD12]
|
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206757L1 | Lenti ORF clone of Human NLR family, CARD domain containing 4 (NLRC4), transcript variant 1, Myc-DDK-tagged |
CNY 9,528.00 |
|
| RC206757L2 | Lenti ORF clone of Human NLR family, CARD domain containing 4 (NLRC4), transcript variant 1, mGFP tagged |
CNY 12,160.00 |
|
| RC206757L3 | Lenti ORF clone of Human NLR family, CARD domain containing 4 (NLRC4), transcript variant 1, Myc-DDK-tagged |
CNY 9,528.00 |
|
| RC206757L4 | Lenti ORF clone of Human NLR family, CARD domain containing 4 (NLRC4), transcript variant 1, mGFP tagged |
CNY 9,528.00 |
|
| RG206757 | NLRC4 (tGFP-tagged) - Human NLR family, CARD domain containing 4 (NLRC4), transcript variant 1 |
CNY 8,728.00 |
|
| SC118775 | NLRC4 (untagged)-Human NLR family, CARD domain containing 4 (NLRC4), transcript variant 1 |
CNY 7,136.00 |
