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Fibromodulin (FMOD) (NM_002023) Human Tagged ORF Clone
CAT＃: RC206534
FMOD (Myc-DDK-tagged)-Human fibromodulin (FMOD)
ORF Plasmid: tGFP
"NM_002023" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FM; SLRR2E |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206534 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTGGACCTCCCTCCTGCTGCTGGCAGGGCTCTTCTCCCTCTCCCAGGCCCAGTATGAAGATGACC CTCATTGGTGGTTCCACTACCTCCGCAGCCAGCAGTCCACCTACTACGATCCCTATGACCCTTACCCGTA TGAGACCTACGAGCCTTACCCCTATGGGGTGGATGAAGGGCCAGCCTACACCTACGGCTCTCCATCCCCT CCAGATCCCCGCGACTGCCCCCAGGAGTGCGACTGCCCACCCAACTTCCCCACGGCCATGTACTGTGACA ATCGCAACCTCAAGTACCTGCCCTTCGTTCCCTCCCGCATGAAGTATGTGTACTTCCAGAACAACCAGAT CACCTCCATCCAGGAAGGCGTCTTTGACAATGCCACAGGGCTGCTCTGGATTGCTCTCCACGGCAACCAG ATCACCAGTGATAAGGTGGGCAGGAAGGTCTTCTCCAAGCTGAGGCACCTGGAGAGGCTGTACCTGGACC ACAACAACCTGACCCGGATGCCCGGTCCCCTGCCTCGATCCCTGAGAGAGCTCCATCTCGACCACAACCA GATCTCACGGGTCCCCAACAATGCTCTGGAGGGGCTGGAGAACCTCACGGCCTTGTACCTCCAACACAAT GAGATCCAGGAAGTGGGCAGTTCCATGAGGGGCCTCCGGTCACTGATCTTGCTGGACCTGAGTTATAACC ACCTTCGGAAGGTGCCTGATGGGCTGCCCTCAGCTCTTGAGCAGCTGTACATGGAGCACAACAATGTCTA CACCGTCCCCGATAGCTACTTCCGGGGGGCGCCCAAGCTGCTGTATGTGCGGCTGTCCCACAACAGTCTA ACCAACAATGGCCTGGCCTCCAACACCTTCAATTCCAGCAGCCTCCTTGAGCTAGACCTCTCCTACAACC AGCTGCAGAAGATCCCCCCAGTCAACACCAACCTGGAGAACCTCTACCTCCAAGGCAATAGGATCAATGA GTTCTCCATCAGCAGCTTCTGCACCGTGGTGGACGTCGTGAACTTCTCCAAGCTGCAGGTGCTGCGCCTG GACGGGAACGAGATCAAGCGCAGCGCCATGCCTGCCGACGCGCCCCTCTGCCTGCGCCTTGCCAGCCTCA TCGAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206534 protein sequence
Red=Cloning site Green=Tags(s) MQWTSLLLLAGLFSLSQAQYEDDPHWWFHYLRSQQSTYYDPYDPYPYETYEPYPYGVDEGPAYTYGSPSP PDPRDCPQECDCPPNFPTAMYCDNRNLKYLPFVPSRMKYVYFQNNQITSIQEGVFDNATGLLWIALHGNQ ITSDKVGRKVFSKLRHLERLYLDHNNLTRMPGPLPRSLRELHLDHNQISRVPNNALEGLENLTALYLQHN EIQEVGSSMRGLRSLILLDLSYNHLRKVPDGLPSALEQLYMEHNNVYTVPDSYFRGAPKLLYVRLSHNSL TNNGLASNTFNSSSLLELDLSYNQLQKIPPVNTNLENLYLQGNRINEFSISSFCTVVDVVNFSKLQVLRL DGNEIKRSAMPADAPLCLRLASLIEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002023 |
| ORF Size | 1128 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002023.5 |
| RefSeq Size | 3271 bp |
| RefSeq ORF | 1131 bp |
| Locus ID | 2331 |
| UniProt ID | Q06828 |
| Domains | LRRNT, LRR, LRR_TYP, LRR_BAC, LRR_PS |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 43.2 kDa |
| Gene Summary | Fibromodulin belongs to the family of small interstitial proteoglycans. The encoded protein possesses a central region containing leucine-rich repeats with 4 keratan sulfate chains, flanked by terminal domains containing disulphide bonds. Owing to the interaction with type I and type II collagen fibrils and in vitro inhibition of fibrillogenesis, the encoded protein may play a role in the assembly of extracellular matrix. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. Sequence variations in this gene may be associated with the pathogenesis of high myopia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206534L1 | Lenti ORF clone of Human fibromodulin (FMOD), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206534L2 | Lenti ORF clone of Human fibromodulin (FMOD), mGFP tagged |
CNY 5,890.00 |
|
| RC206534L3 | Lenti ORF clone of Human fibromodulin (FMOD), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206534L4 | Lenti ORF clone of Human fibromodulin (FMOD), mGFP tagged |
CNY 5,890.00 |
|
| RG206534 | FMOD (tGFP-tagged) - Human fibromodulin (FMOD) |
CNY 4,370.00 |
|
| SC321707 | FMOD (untagged)-Human fibromodulin (FMOD) |
CNY 3,656.00 |
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