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EID3 (NM_001008394) Human Tagged ORF Clone
CAT＃: RC205575
EID3 (Myc-DDK-tagged)-Human EP300 interacting inhibitor of differentiation 3 (EID3)
ORF Plasmid: tGFP
"NM_001008394" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NS4EB; NSE4B; NSMCE4B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205575 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATGGATGTGTCAGTGAGGGCCGCGGGCTGCTCCGACGACCTCAGCTCTGGGGAGGCCGACGTAG ACCCAAAGCTCCTGGAGCTCACCGCTGACGAGGAGAAGTGCCGCAGCATCCGCAGGCAGTACCGGCAGCT CATGTACTGCGTGCGGCAGAACCGGGAGGACATCGTGAGCTCGGCGAACAACTCCTTAACCGAGGCTCTG GAGGAAGCCAACGTCCTCTTTGATGGCGTGAGCCGAACCAGAGAAGCAGCCCTCGACGCCCGGTTTCTTG TTATGGCTTCTGATTTGGGTAAAGAAAAGGCAAAGCAGTTAAACTCAGATATGAACTTCTTTAATCAGTT AGCATTTTGTGACTTTCTGTTTCTGTTCGTGGGTCTGAATTGGATGGAAGGCGATCCTGACAAGTTGAGT GATTGTGATGATAGCATAGCTCTTTCCTTCTGGAAGGCAATAGAAAAGGAAGCAACATCCTGGATGGTAA AAGCTGAGACATTCCATTTTGTTTTTGGTTCATTCAAGCTAGAACGTTCTGCACCAAAGCCCCGACTTGA ACACCAGAAAAAAGTTCGCAAGATGGAAGAAAATGGCAACATGCCTACAAAGTTGCAGAAGTTGGACCTG AGTAGTTATCCAGAAGCGACAGAAAAAAACGTAGAAAGGATTTTGGGATTGTTGCAAACCTACTTTCGAA AGTATCCTGATACTCCTGTGTCCTATTTTGAGTTTGTGATTGATCCAAACTCTTTTTCTCGTACTGTGGA GAATATATTTTATGTTTCTTTTATTGTAAGAGATGGTTTTGCAAGAATAAGGCTTGATGAAGACAGGCTG CCAATATTAGAGCCGATGAATGTTAACCAAATGGGTGAGGGAAATGATTCCAGTTGCCATGGCAGGAAAC AGGGAGTTATATCTTTGACTTTACAGGAGTGGAAAAACATTGTGGCAGCTTTTGAAATTTCTGAGGCTAT GATTACATACTCCTCATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205575 protein sequence
Red=Cloning site Green=Tags(s) MKMDVSVRAAGCSDDLSSGEADVDPKLLELTADEEKCRSIRRQYRQLMYCVRQNREDIVSSANNSLTEAL EEANVLFDGVSRTREAALDARFLVMASDLGKEKAKQLNSDMNFFNQLAFCDFLFLFVGLNWMEGDPDKLS DCDDSIALSFWKAIEKEATSWMVKAETFHFVFGSFKLERSAPKPRLEHQKKVRKMEENGNMPTKLQKLDL SSYPEATEKNVERILGLLQTYFRKYPDTPVSYFEFVIDPNSFSRTVENIFYVSFIVRDGFARIRLDEDRL PILEPMNVNQMGEGNDSSCHGRKQGVISLTLQEWKNIVAAFEISEAMITYSSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001008394 |
| ORF Size | 999 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001008394.3 |
| RefSeq Size | 1486 bp |
| RefSeq ORF | 1002 bp |
| Locus ID | 493861 |
| UniProt ID | Q8N140 |
| MW | 38.2 kDa |
| Gene Summary | Tissue-specific component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205575L3 | Lenti ORF clone of Human EP300 interacting inhibitor of differentiation 3 (EID3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205575L4 | Lenti ORF clone of Human EP300 interacting inhibitor of differentiation 3 (EID3), mGFP tagged |
CNY 5,890.00 |
|
| RG205575 | EID3 (tGFP-tagged) - Human EP300 interacting inhibitor of differentiation 3 (EID3) |
CNY 4,000.00 |
|
| SC301286 | EID3 (untagged)-Human EP300 interacting inhibitor of differentiation 3 (EID3) |
CNY 3,656.00 |
