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HIPPI (IFT57) (NM_018010) Human Tagged ORF Clone
CAT＃: RC204116
IFT57 (Myc-DDK-tagged)-Human intraflagellar transport 57 homolog (Chlamydomonas) (IFT57)
ORF Plasmid: tGFP
"NM_018010" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ESRRBL1; HIPPI; MHS4R2; OFD18 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204116 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGCTGCTCTGGCCGTCGTCACGACGTCGGGTTTGGAAGATGGGGTGCCTAGGTCCCGTGGCGAAG GGACCGGGGAAGTGGTCTTGGAGCGGGGGCCCGGCGCGGCCTACCACATGTTCGTGGTGATGGAGGACTT GGTGGAGAAGCTGAAGCTGCTCCGCTACGAGGAGGAGTTCCTCCGGAAGAGCAACCTGAAGGCCCCGTCC AGACACTATTTTGCACTGCCTACCAACCCTGGCGAACAGTTCTACATGTTTTGTACTCTTGCTGCTTGGT TGATTAATAAAGCGGGACGTCCCTTTGAGCAGCCTCAAGAATATGATGACCCTAATGCAACAATATCTAA CATACTATCCGAGCTTCGGTCATTTGGAAGAACTGCAGATTTTCCTCCTTCAAAATTAAAGTCAGGTTAT GGAGAACATGTATGCTATGTTCTTGATTGCTTCGCTGAAGAAGCATTGAAATATATTGGTTTCACCTGGA AAAGGCCAATATACCCAGTAGAAGAATTAGAAGAAGAAAGCGTTGCAGAAGATGATGCAGAATTAACATT AAATAAAGTGGATGAAGAATTTGTGGAAGAAGAGACAGATAATGAAGAAAACTTTATTGATCTCAACGTT TTAAAGGCCCAGACATATCACTTGGATATGAACGAGACTGCCAAACAAGAAGATATTTTGGAATCCACAA CAGATGCTGCAGAATGGAGCCTAGAAGTGGAACGTGTACTACCGCAACTGAAAGTCACGATTAGGACTGA CAATAAGGATTGGAGAATCCATGTTGACCAAATGCACCAGCACAGAAGTGGAATTGAATCTGCTCTAAAG GAGACCAAGGGATTTTTGGACAAACTCCATAATGAAATTACTAGGACTTTGGAAAAGATCAGCAGCCGAG AAAAGTACATCAACAATCAGCTTGAGAATTTGGTTCAAGAATATCGTGCAGCTCAAGCCCAGCTGAGTGA GGCAAAGGAGCGATACCAGCAGGGAAATGGAGGAGTGACGGAAAGAACCAGACTCCTCTCTGAGGTTATG GAAGAATTAGAAAAGGTAAAACAAGAAATGGAAGAAAAGGGCAGCAGCATGACTGATGGTGCTCCTTTGG TGAAGATTAAACAGAGCTTAACAAAACTGAAGCAAGAAACTGTAGAGATGGACATTAGAATTGGCATTGT GGAACACACACTACTCCAATCAAAGCTGAAGGAGAAGTCCAACATGACTAGGAACATGCATGCCACAGTT ATTCCAGAACCAGCAACAGGCTTTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204116 protein sequence
Red=Cloning site Green=Tags(s) MTAALAVVTTSGLEDGVPRSRGEGTGEVVLERGPGAAYHMFVVMEDLVEKLKLLRYEEEFLRKSNLKAPS RHYFALPTNPGEQFYMFCTLAAWLINKAGRPFEQPQEYDDPNATISNILSELRSFGRTADFPPSKLKSGY GEHVCYVLDCFAEEALKYIGFTWKRPIYPVEELEEESVAEDDAELTLNKVDEEFVEEETDNEENFIDLNV LKAQTYHLDMNETAKQEDILESTTDAAEWSLEVERVLPQLKVTIRTDNKDWRIHVDQMHQHRSGIESALK ETKGFLDKLHNEITRTLEKISSREKYINNQLENLVQEYRAAQAQLSEAKERYQQGNGGVTERTRLLSEVM EELEKVKQEMEEKGSSMTDGAPLVKIKQSLTKLKQETVEMDIRIGIVEHTLLQSKLKEKSNMTRNMHATV IPEPATGFY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018010 |
| ORF Size | 1287 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018010.4 |
| RefSeq Size | 3223 bp |
| RefSeq ORF | 1290 bp |
| Locus ID | 55081 |
| UniProt ID | Q9NWB7 |
| Protein Families | Druggable Genome |
| Protein Pathways | Huntington's disease |
| MW | 49.1 kDa |
| Gene Summary | Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204116L3 | Lenti ORF clone of Human intraflagellar transport 57 homolog (Chlamydomonas) (IFT57), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204116L4 | Lenti ORF clone of Human intraflagellar transport 57 homolog (Chlamydomonas) (IFT57), mGFP tagged |
CNY 5,890.00 |
|
| RG204116 | IFT57 (tGFP-tagged) - Human intraflagellar transport 57 homolog (Chlamydomonas) (IFT57) |
CNY 4,370.00 |
|
| SC321611 | IFT57 (untagged)-Human intraflagellar transport 57 homolog (Chlamydomonas) (IFT57) |
CNY 2,950.00 |
