United States
Germany
Japan
United Kingdom
China
Dysadherin (FXYD5) (NM_144779) Human Tagged ORF Clone
CAT＃: RC203917
FXYD5 (Myc-DDK-tagged)-Human FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 1
ORF Plasmid: tGFP
"NM_144779" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DYSAD; HSPC113; IWU1; KCT1; OIT2; PRO6241; RIC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203917 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCCCTCTGGTCGCCTGTGTCTTCTTACCATCGTTGGCCTGATTCTCCCCACCAGAGGACAGACGT TGAAAGATACCACGTCCAGTTCTTCAGCAGACTCAACTATCATGGACATTCAGGTCCCGACACGAGCCCC AGATGCAGTCTACACAGAACTCCAGCCCACCTCTCCAACCCCAACCTGGCCTGCTGATGAAACACCACAA CCCCAGACCCAGACCCAGCAACTGGAAGGAACGGATGGGCCTCTAGTGACAGATCCAGAGACACACAAGA GCACCAAAGCAGCTCATCCCACTGATGACACCACGACGCTCTCTGAGAGACCATCCCCAAGCACAGACGT CCAGACAGACCCCCAGACCCTCAAGCCATCTGGTTTTCATGAGGATGACCCCTTCTTCTATGATGAACAC ACCCTCCGGAAACGGGGGCTGTTGGTCGCAGCTGTGCTGTTCATCACAGGCATCATCATCCTCACCAGTG GCAAGTGCAGGCAGCTGTCCCGGTTATGCCGGAATCATTGCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203917 protein sequence
Red=Cloning site Green=Tags(s) MSPSGRLCLLTIVGLILPTRGQTLKDTTSSSSADSTIMDIQVPTRAPDAVYTELQPTSPTPTWPADETPQ PQTQTQQLEGTDGPLVTDPETHKSTKAAHPTDDTTTLSERPSPSTDVQTDPQTLKPSGFHEDDPFFYDEH TLRKRGLLVAAVLFITGIIILTSGKCRQLSRLCRNHCR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144779 |
| ORF Size | 534 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144779.3 |
| RefSeq Size | 927 bp |
| RefSeq ORF | 537 bp |
| Locus ID | 53827 |
| UniProt ID | Q96DB9 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| MW | 19.5 kDa |
| Gene Summary | This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product, FXYD5, is a glycoprotein that functions in the up-regulation of chemokine production, and it is involved in the reduction of cell adhesion via its ability to down-regulate E-cadherin. It also promotes metastasis, and has been linked to a variety of cancers. Alternative splicing results in multiple transcript variants. [RefSeq curation by Kathleen J. Sweadner, Ph.D., sweadner@helix.mgh.harvard.edu., Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203917L3 | Lenti-ORF clone of FXYD5 (Myc-DDK-tagged)-Human FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 1 |
CNY 5,890.00 |
|
| RC203917L4 | Lenti-ORF clone of FXYD5 (mGFP-tagged)-Human FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 1 |
CNY 5,890.00 |
|
| RG203917 | FXYD5 (tGFP-tagged) - Human FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 1 |
CNY 4,000.00 |
|
| SC127643 | FXYD5 (untagged)-Human FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 1 |
CNY 2,400.00 |
其它Dysadherin产品
