ZFYVE21 (NM_024071) Human Tagged ORF Clone

CAT#: RC203337

ZFYVE21 (Myc-DDK-tagged)-Human zinc finger, FYVE domain containing 21 (ZFYVE21), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_024071" in other vectors (4)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


ZFYVE21 mouse monoclonal antibody,clone OTI4A2
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HCVP7TP1; ZF21
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203337 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTCCGAGGTGTCCGCGCGCCGCGACGCCAAGAAGCTGGTGCGCTCCCCGAGCGGCCTGCGCATGG
TGCCCGAACACCGCGCCTTCGGAAGCCCGTTCGGCCTGGAGGAGCCGCAGTGGGTCCCGGACAAGGAGTG
TCGGAGATGTATGCAGTGTGACGCCAAGTTTGACTTTCTCACCAGAAAGCACCACTGTCGCCGCTGCGGG
AAGTGCTTCTGCGACAGGTGCTGCAGCCAGAAGGTGCCGCTGCGGCGCATGTGCTTTGTGGACCCCGTGC
GGCAGTGCGCGGAGTGCGCCCTCGTGTCCCTCAAGGAGGCGGAGTTCTACGACAAGCAGCTCAAAGTGCT
CCTGAGCGGAGCCACCTTCCTCGTCACGTTTGGAAACTCAGAGAAACCTGAAACTATGACTTGTCGTCTT
TCCAATAACCAGAGATACTTGTTTCTGGATGGAGACAGCCACTATGAAATCGAAATTGTACACATTTCCA
CCGTGCAGATCCTCACAGAAGGCTTCCCTCCTGGAGGAGGCAACGCACGGGCCACAGGCATGTTCCTGCA
GTATACAGTGCCGGGGACGGAGGGTGTGACCCAGCTGAAGCTGACAGTGGTGGAGGACGTGACTGTGGGC
AGGAGGCAGGCGGTGGCGTGGCTAGTGGCCATGCACAAGGCTGCCAAGCTCCTCTATGAATCTCGGGACC
AG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203337 protein sequence
Red=Cloning site Green=Tags(s)

MSSEVSARRDAKKLVRSPSGLRMVPEHRAFGSPFGLEEPQWVPDKECRRCMQCDAKFDFLTRKHHCRRCG
KCFCDRCCSQKVPLRRMCFVDPVRQCAECALVSLKEAEFYDKQLKVLLSGATFLVTFGNSEKPETMTCRL
SNNQRYLFLDGDSHYEIEIVHISTVQILTEGFPPGGGNARATGMFLQYTVPGTEGVTQLKLTVVEDVTVG
RRQAVAWLVAMHKAAKLLYESRDQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024071
ORF Size 702 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024071.4
RefSeq Size 1470 bp
RefSeq ORF 705 bp
Locus ID 79038
UniProt ID Q9BQ24
Domains FYVE
MW 26.5 kDa
Gene Summary Plays a role in cell adhesion, and thereby in cell motility which requires repeated formation and disassembly of focal adhesions. Regulates microtubule-induced PTK2/FAK1 dephosphorylation, an event important for focal adhesion disassembly, as well as integrin beta-1/ITGB1 cell surface expression.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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