fast skeletal Myosin (MYLPF) (NM_013292) Human Tagged ORF Clone
CAT#: RC203081
MYLPF (Myc-DDK-tagged)-Human myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF)
ORF Plasmid: tGFP
"NM_013292" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HUMMLC2B; MLC2B; MRLC2; MYL11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203081 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCCAAGAGGGCCAAGAGAAGGACAGTAGAGGGCGGAAGCTCCAGCGTCTTCTCCATGTTCGACC AGACTCAGATCCAGGAGTTCAAAGAGGCCTTCACTGTGATCGACCAGAACCGTGATGGTATTATAGACAA GGAGGACCTTCGGGACACCTTCGCAGCCATGGGCCGCCTCAATGTGAAGAATGAGGAGTTGGATGCCATG ATGAAGGAAGCCAGCGGTCCCATCAACTTCACCGTCTTCCTGACCATGTTCGGGGAGAAGCTCAAGGGTG CCGACCCTGAGGATGTGATCACCGGAGCCTTCAAGGTCTTGGACCCTGAGGGAAAGGGCACCATCAAGAA GAAGTTCCTGGAGGAGCTGCTGACCACGCAGTGTGACCGCTTCTCCCAGGAGGAGATCAAGAACATGTGG GCGGCCTTCCCCCCCGACGTGGGCGGCAACGTCGACTACAAAAACATCTGCTACGTCATCACGCACGGCG ACGCCAAGGACCAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203081 protein sequence
Red=Cloning site Green=Tags(s) MAPKRAKRRTVEGGSSSVFSMFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAM MKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKKFLEELLTTQCDRFSQEEIKNMW AAFPPDVGGNVDYKNICYVITHGDAKDQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013292 |
ORF Size | 507 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013292.5 |
RefSeq Size | 683 bp |
RefSeq ORF | 510 bp |
Locus ID | 29895 |
UniProt ID | Q96A32 |
Protein Pathways | Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction |
MW | 19 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203081L3 | Lenti ORF clone of Human myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203081L4 | Lenti ORF clone of Human myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), mGFP tagged |
CNY 5,890.00 |
|
RG203081 | MYLPF (tGFP-tagged) - Human myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF) |
CNY 4,000.00 |
|
SC122791 | MYLPF (untagged)-Human myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF) |
CNY 2,400.00 |
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