TACO1 (NM_016360) Human Tagged ORF Clone

CAT#: RC202948

TACO1 (Myc-DDK-tagged)-Human translational activator of mitochondrially encoded cytochrome c oxidase I (TACO1), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP



  "NM_016360" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


TACO1 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CCDC44; MC4DN8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202948 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCTTGGGCTGCTGCCAGCCTAAGCAGGGCCGCTGCCCGATGCTTGCTGGCACGAGGCCCCGGGG
TCAGGGCGGCTCCTCCGCGCGACCCCCGGCCCTCCCACCCCGAGCCCCGGGGCTGCGGTGCCGCTCCGGG
CAGGACGCTGCACTTTACCGCGGCTGTCCCCGCCGGGCACAACAAGTGGTCCAAAGTCAGGCACATCAAG
GGTCCGAAGGACGTCGAAAGGAGTCGCATCTTCTCCAAACTCTGTTTGAACATCCGCCTGGCAGTGAAAG
AAGGAGGCCCCAACCCTGAGCACAACAGCAACCTGGCCAATATCTTAGAGGTGTGTCGCAGCAAACATAT
GCCCAAGTCAACGATTGAGACAGCACTGAAAATGGAGAAATCCAAGGACACTTATTTGCTGTATGAGGGT
CGAGGCCCTGGTGGCTCTTCTCTGCTCATCGAGGCATTATCTAACAGTAGCCACAAGTGCCAAGCAGACA
TTAGACATATCCTGAATAAGAATGGAGGAGTGATGGCTGTAGGAGCTCGTCACTCTTTTGACAAAAAGGG
GGTGATTGTGGTTGAAGTGGAGGACAGAGAGAAGAAGGCTGTGAACCTAGAGCGTGCCCTGGAGATGGCA
ATCGAAGCAGGAGCTGAGGATGTCAAGGAAACTGAAGATGAAGAAGAAAGGAACGTTTTTAAATTTATTT
GTGATGCCTCTTCACTGCACCAAGTGAGGAAGAAGCTGGACTCCCTGGGCCTGTGTTCTGTGTCCTGTGC
ACTAGAGTTCATCCCCAACTCAAAGGTGCAGCTGGCTGAGCCCGACCTGGAACAGGCCGCACATCTCATT
CAGGCTCTCAGCAACCACGAGGATGTGATTCACGTCTATGATAACATTGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202948 protein sequence
Red=Cloning site Green=Tags(s)

MSAWAAASLSRAAARCLLARGPGVRAAPPRDPRPSHPEPRGCGAAPGRTLHFTAAVPAGHNKWSKVRHIK
GPKDVERSRIFSKLCLNIRLAVKEGGPNPEHNSNLANILEVCRSKHMPKSTIETALKMEKSKDTYLLYEG
RGPGGSSLLIEALSNSSHKCQADIRHILNKNGGVMAVGARHSFDKKGVIVVEVEDREKKAVNLERALEMA
IEAGAEDVKETEDEEERNVFKFICDASSLHQVRKKLDSLGLCSVSCALEFIPNSKVQLAEPDLEQAAHLI
QALSNHEDVIHVYDNIE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016360
ORF Size 891 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016360.4
RefSeq Size 1479 bp
RefSeq ORF 894 bp
Locus ID 51204
UniProt ID Q9BSH4
Domains DUF28
MW 32.5 kDa
Gene Summary This gene encodes a mitochondrial protein that function as a translational activator of mitochondrially-encoded cytochrome c oxidase 1. Mutations in this gene are associated with Leigh syndrome.[provided by RefSeq, Mar 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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