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PTDSS1 (NM_014754) Human Tagged ORF Clone
CAT＃: RC201012
PTDSS1 (Myc-DDK-tagged)-Human phosphatidylserine synthase 1 (PTDSS1)
ORF Plasmid: tGFP
"NM_014754" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LMHD; PSS1; PSSA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201012 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCTGCGTGGGGAGCCGGACCCTAAGCAAGGATGATGTGAACTACAAAATGCATTTCCGGATGA TCAACGAGCAGCAAGTGGAGGACATCACCATTGACTTCTTCTACCGGCCGCATACCATCACCCTGCTCAG CTTCACCATCGTCAGCCTCATGTACTTCGCCTTTACCAGGGATGACTCTGTTCCAGAAGACAACATCTGG AGAGGCATCCTCTCTGTTATTTTCTTCTTTCTTATCATCAGTGTGTTAGCTTTCCCCAATGGTCCGTTCA CTCGACCTCATCCAGCCTTATGGCGAATGGTTTTTGGACTCAGTGTGCTCTACTTCCTGTTCCTGGTATT CCTACTCTTCCTGAATTTCGAGCAGGTTAAATCTCTAATGTATTGGCTAGATCCAAATCTTCGATACGCC ACAAGGGAAGCAGATGTCATGGAGTATGCTGTGAACTGCCATGTGATCACCTGGGAGAGGATTATCAGCC ACTTTGATATTTTTGCATTTGGACATTTCTGGGGCTGGGCCATGAAGGCCTTGCTGATCCGTAGTTACGG TCTCTGCTGGACAATCAGTATTACCTGGGAGCTGACTGAGCTCTTCTTCATGCATCTCCTCCCCAATTTT GCCGAGTGCTGGTGGGATCAAGTCATTCTGGACATCCTGTTGTGCAATGGCGGTGGCATTTGGCTGGGCA TGGTCGTTTGCCGGTTTTTAGAGATGAGGACTTACCACTGGGCAAGCTTCAAGGACATTCATACCACCAC CGGGAAGATCAAGAGAGCTGTTCTGCAGTTCACTCCTGCTAGCTGGACCTATGTTCGATGGTTTGACCCC AAATCTTCTTTTCAGAGAGTAGCTGGAGTGTACCTTTTCATGATCATCTGGCAGCTGACTGAGTTGAATA CCTTCTTCTTGAAGCATATCTTTGTGTTCCAAGCCAGTCATCCATTAAGTTGGGGTAGAATTCTCTTTAT TGGTGGCATCACAGCTCCCACAGTGAGACAGTACTACGCTTACCTCACCGACACACAGTGCAAGCGCGTA GGAACACAATGCTGGGTGTTTGGGGTCATTGGTTTCCTGGAGGCCATTGTTTGCATAAAATTTGGACAAG ATCTCTTCTCTAAGACCCAAATACTCTATGTTGTGCTTTGGCTTCTTTGCGTGGCTTTCACCACTTTCCT CTGTCTGTACGGCATGATTTGGTATGCAGAACACTATGGTCACCGAGAAAAGACCTACTCGGAGTGTGAA GATGGCACCTACAGTCCAGAGATCTCCTGGCATCACAGGAAAGGGACAAAAGGTTCTGAAGACAGCCCAC CCAAGCATGCAGGCAACAACGAAAGCCATTCTTCCAGGAGAAGGAATCGGCATTCCAAGTCAAAAGTCAC CAATGGCGTTGGAAAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201012 protein sequence
Red=Cloning site Green=Tags(s) MASCVGSRTLSKDDVNYKMHFRMINEQQVEDITIDFFYRPHTITLLSFTIVSLMYFAFTRDDSVPEDNIW RGILSVIFFFLIISVLAFPNGPFTRPHPALWRMVFGLSVLYFLFLVFLLFLNFEQVKSLMYWLDPNLRYA TREADVMEYAVNCHVITWERIISHFDIFAFGHFWGWAMKALLIRSYGLCWTISITWELTELFFMHLLPNF AECWWDQVILDILLCNGGGIWLGMVVCRFLEMRTYHWASFKDIHTTTGKIKRAVLQFTPASWTYVRWFDP KSSFQRVAGVYLFMIIWQLTELNTFFLKHIFVFQASHPLSWGRILFIGGITAPTVRQYYAYLTDTQCKRV GTQCWVFGVIGFLEAIVCIKFGQDLFSKTQILYVVLWLLCVAFTTFLCLYGMIWYAEHYGHREKTYSECE DGTYSPEISWHHRKGTKGSEDSPPKHAGNNESHSSRRRNRHSKSKVTNGVGKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014754 |
| ORF Size | 1419 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014754.3 |
| RefSeq Size | 2576 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 9791 |
| UniProt ID | P48651 |
| Domains | PSS |
| Protein Families | Transmembrane |
| Protein Pathways | Glycerophospholipid metabolism, Metabolic pathways |
| MW | 55.5 kDa |
| Gene Summary | The protein encoded by this gene catalyzes the formation of phosphatidylserine from either phosphatidylcholine or phosphatidylethanolamine. Phosphatidylserine localizes to the mitochondria-associated membrane of the endoplasmic reticulum, where it serves a structural role as well as a signaling role. Defects in this gene are a cause of Lenz-Majewski hyperostotic dwarfism. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Gain-of-function mutations in the phosphatidylserine synthase 1 (PTDSS1) gene cause Lenz-Majewski syndrome.
,null,
Nature genetics
,PubMed ID 24241535
[PTDSS1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201012L1 | Lenti ORF clone of Human phosphatidylserine synthase 1 (PTDSS1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201012L2 | Lenti ORF clone of Human phosphatidylserine synthase 1 (PTDSS1), mGFP tagged |
CNY 5,890.00 |
|
| RC201012L3 | Lenti ORF clone of Human phosphatidylserine synthase 1 (PTDSS1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201012L4 | Lenti ORF clone of Human phosphatidylserine synthase 1 (PTDSS1), mGFP tagged |
CNY 5,890.00 |
|
| RG201012 | PTDSS1 (tGFP-tagged) - Human phosphatidylserine synthase 1 (PTDSS1) |
CNY 4,370.00 |
|
| SC114852 | PTDSS1 (untagged)-Human phosphatidylserine synthase 1 (PTDSS1) |
CNY 2,950.00 |
