IFI35 (NM_005533) Human Tagged ORF Clone

CAT#: RC200929

IFI35 (Myc-DDK-tagged)-Human interferon-induced protein 35 (IFI35)

ORF Plasmid: DDK tGFP



  "NM_005533" in other vectors (7)

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CNY 2,400.00

CNY 4,465.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


IFI35 mouse monoclonal antibody, clone OTI1C9 (formerly 1C9)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms IFP35
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200929 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGCCCCACTGGATGCCGCCCTCCACGCCCTTCAGGAGGAGCAGGCCAGACTCAAGATGAGGCTGT
GGGACCTGCAGCAGCTGAGAAAGGAGCTCGGGGACTCCCCCAAAGACAAGGTCCCATTTTCAGTGCCCAA
GATCCCCCTGGTATTCCGAGGACACACCCAGCAGGACCCGGAAGTGCCTAAGTCTTTAGTTTCCAATTTG
CGGATCCACTGCCCTCTGCTTGCGGGCTCTGCTCTGATCACCTTTGATGACCCCAAAGTGGCTGAGCAGG
TGCTGCAACAAAAGGAGCACACGATCAACATGGAGGAGTGCCGGCTGCGGGTGCAGGTCCAGCCCTTGGA
GCTGCCCATGGTCACCACCATCCAGGTGATGGTGTCCAGCCAGTTGAGTGGCCGGAGGGTGTTGGTCACT
GGATTTCCTGCCAGCCTCAGGCTGAGTGAGGAGGAGCTGCTGGACAAGCTAGAGATCTTCTTTGGCAAGA
CTAGGAACGGAGGTGGCGATGTGGACGTTCGGGAGCTACTGCCAGGGAGTGTCATGCTGGGGTTTGCTAG
GGATGGAGTGGCTCAGCGTCTGTGCCAAATCGGCCAGTTCACAGTGCCACTGGGTGGGCAGCAAGTCCCT
CTGAGAGTCTCTCCGTATGTGAATGGGGAGATCCAGAAGGCTGAGATCAGGTCGCAGCCAGTTCCCCGCT
CGGTACTGGTGCTCAACATTCCTGATATCTTGGATGGCCCGGAGCTGCATGACGTCCTGGAGATCCACTT
CCAGAAGCCCACCCGCGGGGGCGGGGAGGTAGAGGCCCTGACAGTCGTACCCCAAGGACAGCAGGGCCTA
GCAGTCTTCACCTCTGAGTCAGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200929 protein sequence
Red=Cloning site Green=Tags(s)

MSAPLDAALHALQEEQARLKMRLWDLQQLRKELGDSPKDKVPFSVPKIPLVFRGHTQQDPEVPKSLVSNL
RIHCPLLAGSALITFDDPKVAEQVLQQKEHTINMEECRLRVQVQPLELPMVTTIQVMVSSQLSGRRVLVT
GFPASLRLSEEELLDKLEIFFGKTRNGGGDVDVRELLPGSVMLGFARDGVAQRLCQIGQFTVPLGGQQVP
LRVSPYVNGEIQKAEIRSQPVPRSVLVLNIPDILDGPELHDVLEIHFQKPTRGGGEVEALTVVPQGQQGL
AVFTSESG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005533
ORF Size 864 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005533.2, NP_005524.1
RefSeq Size 1260 bp
RefSeq ORF 867 bp
Locus ID 3430
UniProt ID P80217
MW 31.7 kDa
Gene Summary Not yet known.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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