Jarid2 (NM_001205043) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Jmj; jumonji
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231707 representing NM_001205043 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAAGGAAAGACCCAAGAGGAATATCATTCAGAAGAAATACGATGACAGCGATGGGATCCCGTGGT CAGAAGAGAGAGTTGTACGAAAAGTCCTGTATTTGTCCCTAAAGGAATTCAAGAATGCACAGAAAAGGCA GCATGGGGAAGGCCTTGCCGGGAGCCTGAAGGCGGTAAATGGGCTTCTTGGTAATGCCCAGGCTAAGGCA CTAGGACCAGCCTCAGAGCAGTCAGAGAACGAGAAGGATGATGCCTCCCAAGTGTCCTCTACTAGCAACG ATGTTAGTTCTTCAGATTTTGAAGAAGGGCCGTCGAGGAAAAGGCCCAGGCTGCAAGCACAAAGGAAGTT TGCTCAATCTCAGCCGAATAGTCCCAGCACAACTCCAGTGAAGATAGTGGAGCCTTTGCTACCCCCGCCA GCTACTCAAATTTCTGACCTCTCTAAAAGGAAGCCTAAGACAGAAGACTTTCTTACCTTTCTCTGCCTTC GAGGTTCTCCTGCGCTGCCCAACAGTATGGTATATTTTGGAAGCTCTCAGGATGAGGAGGATGTCGAAGA GGAAGATGATGAGACGGAAGATGTCAAAGCAACCACCAACAATGCTTCATCTTCGTGCCAGTCAACCCCC AGGAAAGGAAAAACCCATAAGCATGTACACAACGGGCACGTTTTCAATGGCTCCAGTAGGTCAGCACGGG AGAAAGAGCCTGCTCACAAACACAGAAGCAAAGAGGCCACTCCGGGGAAGGAGAAGCACAGCGAGCCCAG GGCAGACAGCCGGAGAGAACAGGCTTCAGGGGCTCAGCCCACGGCCGCCTCGGCTGCGGCTTCCTCTGCC AAGGGACTTGCTGCCAACCACCAACCTCCCCCTTCGCATCGGTCTGCTCAGGACTTACGGAAACAGGTGT CTAAGGTAAATGGAGTCACTCGAATGTCATCTCTGGGTGCAGGTACAAACAGTGCCAAAAAGATCCGAGA AGTCAGACCTTCACCATCCAAAACTGTGAAGTACACTGCCACAGTGACCAAGGGGACTGTCACATACACC AAAGCCAAGAGAGAACTGGTCAAGGAAACCAAACCCAACCACCACAAACCCAGTTCAGCTGTCAACCACA CAATCTCAGGGAAAACTGAAAGTAGCAATGCAAAAACCCGCAAACAGGTGCTATCCCTTGGGGGGGCGTC CAAGTCCACCGGGCCTGCTGCCAGTGGCCTCAAGGCCAGCAGCAGGTTGAACCCAAAGTCATGCACTAAG GAGGTGGGGGGGCGGCAGCTGAGGGAGGGGCTGCGGAATTCCAAAAGGAGACTGGAAGAGGCACAGCAAG TGGACAAGCCGCAGTCACCCCCCAAGAAGATGAAGGGGGTGGCAGGCAATGCTGAAGCCCCTGGCAAAAA GGCCTCAGCGGCTTCAGGAGAAAAGTCTCTGCTGAATGGACACGTGAAGAAGGAAGTGCCCGAGCGAAGT TTGGAAAGGAATCGGCCAAAGCGGGCCGCGGCTGGCAAGAATATGCTGGGCAAACAAGCACATGGCAAGA CAGAGGGCACCCCCTGTGAAAATCGTTCTACCTCGCAACCCGAGTCCTCGCACAAGCCGCATGACCCACA GGGCAAGCCAGAGAAGGGGAGCGGCAAGTCTGGGTGGGCAGCGATGGACGAGATCCCTGTCCTCAGGCCC TCCGCCAAGGAGTTCCACGACCCGCTCATCTACATCGAGTCAGTACGTGCTCAGGTGGAGAAGTACGGGA TGTGTCGCGTGATTCCCCCTCCAGACTGGCGGCCAGAGTGCAAGCTCAACGATGAGATGCGCTTTGTCAC GCAGATCCAGCACATCCATAAGCTGGGCCGGCGCTGGGGCCCCAACGTGCAGCGGCTAGCCTGCATAAAG AAGCACCTCAGATCTCAGGGCATCACCATGGATGAGCTCCCCCTCATAGGAGGCTGTGAGCTCGACCTGG CCTGCTTTTTCCGGCTGATTAATGAGATGGGCGGCATGCAGCAAGTGACTGACCTCAAAAAATGGAACAA ACTAGCAGACATGCTGCGCATTCCCAAAACTGCCCAGGACCGGCTGGCCAAGCTGCAGGAGGCCTACTGC CAGTACCTGCTTTCCTATGACTCCCTGTCCCCCGAGGAGCACCGACGGCTGGAGAAGGAGGTGCTGATGG AGAAGGAGATCCTGGAAAAGCGCAAAGGGCCATTAGAGGGGCACACGGAGAGCGACCACCACAAGTTCCA CTCCCTGCCCCGCTTTGAGCCCAAGAATGGGCTCGTCCACGGTGTCACACCTAGGAATGGATTCCGTAGC AAGCTCAAGGAGGTGGGCCGGGCCCCACTGAAGACGGGCCGGCGGCGGCTGTTCGCTCAGGAAAAAGAAG TGGTCAAAGAGGAGGAAGAAGACAAAGGTGTCCTCAATGACTTCCACAAGTGCATCTATAAGGGAAGATC TGTTTCTCTAACAACTTTTTATCGAACAGCAAGAAACATCATGAACATGTGTTTCAGCAAGGAGCCCGCA CCAGCAGAGATTGAGCAAGAGTACTGGAGGTTAGTGGAAGAGAAGGACTGTCATGTGGCAGTTCACTGCG GAAAGGTGGACACTAATACCCATGGCAGCGGGTTCCCGGTGGGAAAATCAGAACCCTTTTCAAGGCATGG ATGGAACCTCACTGTCCTCCCAAATAACACAGGGTCCATCCTGCGTCACCTTGGTGCTGTGCCTGGAGTG ACTATTCCCTGGCTAAATATTGGCATGGTCTTTTCTACCTCATGCTGGTCTCGAGACCAAAATCACCTTC CATATATTGACTACTTACACACTGGTGCTGACTGCATTTGGTATTGCATTCCTGCTGAGGAGGAGAACAA GCTGGAGGACGTAGTCCACACTCTGCTGCAGGGCAATGGTACCCCAGGGCTGCAGATGCTGGAGAGCAAT GTAATGATCTCCCCAGAGGTGCTGTGCAAGAAGGGGATCAAGGTCCACAGGACTGTACAGCAGAGTGGGC AGTTTGTCGTCTGCTTCCCGGGATCCTTTGTGTCCAAAGTATGTTGTGGCTACAACGTCTCAGAGACAGT GCACTTTGCTACCACCCAGTGGACAAGCATGGGCTTCGAGACTGCCAAGGAGATGAAGCGCCGCCATATA GCTAAGCCATTCTCCATGGAGAAGTTGCTCTACCAGATTGCACAAGCAGAAGCAAAAAAGGAAAATGGTC CCACCCTCAGTACCATCTCAGCCCTTCTGGATGAGCTCAGGGATACAGAGCTGCGGCAGCGCAGGCTGTT GTTCGAGGCTGGCCTTCACTCTTCTGCACGCTATGGCAGCCACGACGGCAACAGCACAGTGGCGGATGGG AAGAAAAAGCCTCGAAAGTGGCTACAGCTGGAAACTTCAGAAAGGAGGTGCCAGATCTGCCAGCACTTGT GCTACCTGTCCATGGTGGTACAGGAGAATGAGAATGTCGTGTTCTGCCTGGAGTGTGCACTGCGCCATGT GGAGAAGCAGAAGTCTTGCCGTGGGCTGAAGCTGATGTACCGCTACGATGAGGAGCAGATCATCAGTTTG GTCAACCAGATATGTGGCAAAGTGTCTGGCAAACACGGTGGCATCGAGAACTGTCTCAACAAACCCACAC CAAAAAGAGGTCCCCGCAAGAGAGCGACCGTGGATGTGCCGCCATCTCGGCTCCCATCCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231707 representing NM_001205043 Red=Cloning site Green=Tags(s) MSKERPKRNIIQKKYDDSDGIPWSEERVVRKVLYLSLKEFKNAQKRQHGEGLAGSLKAVNGLLGNAQAKA LGPASEQSENEKDDASQVSSTSNDVSSSDFEEGPSRKRPRLQAQRKFAQSQPNSPSTTPVKIVEPLLPPP ATQISDLSKRKPKTEDFLTFLCLRGSPALPNSMVYFGSSQDEEDVEEEDDETEDVKATTNNASSSCQSTP RKGKTHKHVHNGHVFNGSSRSAREKEPAHKHRSKEATPGKEKHSEPRADSRREQASGAQPTAASAAASSA KGLAANHQPPPSHRSAQDLRKQVSKVNGVTRMSSLGAGTNSAKKIREVRPSPSKTVKYTATVTKGTVTYT KAKRELVKETKPNHHKPSSAVNHTISGKTESSNAKTRKQVLSLGGASKSTGPAASGLKASSRLNPKSCTK EVGGRQLREGLRNSKRRLEEAQQVDKPQSPPKKMKGVAGNAEAPGKKASAASGEKSLLNGHVKKEVPERS LERNRPKRAAAGKNMLGKQAHGKTEGTPCENRSTSQPESSHKPHDPQGKPEKGSGKSGWAAMDEIPVLRP SAKEFHDPLIYIESVRAQVEKYGMCRVIPPPDWRPECKLNDEMRFVTQIQHIHKLGRRWGPNVQRLACIK KHLRSQGITMDELPLIGGCELDLACFFRLINEMGGMQQVTDLKKWNKLADMLRIPKTAQDRLAKLQEAYC QYLLSYDSLSPEEHRRLEKEVLMEKEILEKRKGPLEGHTESDHHKFHSLPRFEPKNGLVHGVTPRNGFRS KLKEVGRAPLKTGRRRLFAQEKEVVKEEEEDKGVLNDFHKCIYKGRSVSLTTFYRTARNIMNMCFSKEPA PAEIEQEYWRLVEEKDCHVAVHCGKVDTNTHGSGFPVGKSEPFSRHGWNLTVLPNNTGSILRHLGAVPGV TIPWLNIGMVFSTSCWSRDQNHLPYIDYLHTGADCIWYCIPAEEENKLEDVVHTLLQGNGTPGLQMLESN VMISPEVLCKKGIKVHRTVQQSGQFVVCFPGSFVSKVCCGYNVSETVHFATTQWTSMGFETAKEMKRRHI AKPFSMEKLLYQIAQAEAKKENGPTLSTISALLDELRDTELRQRRLLFEAGLHSSARYGSHDGNSTVADG KKKPRKWLQLETSERRCQICQHLCYLSMVVQENENVVFCLECALRHVEKQKSCRGLKLMYRYDEEQIISL VNQICGKVSGKHGGIENCLNKPTPKRGPRKRATVDVPPSRLPSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205043
ORF Size	3702 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205043.1, NP_001191972.1
RefSeq Size	5997 bp
RefSeq ORF	3705 bp
Locus ID	16468
UniProt ID	Q62315
MW	137.4 kDa
Gene Summary	Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.[UniProtKB/Swiss-Prot Function]

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