Hipk1 (NM_001301306) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110062K04Rik; Myak
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231641 representing NM_001301306 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCACAGCTGCAGGTGTTTTCGCCCCCATCAGTGTCGTCGAGTGCCTTCTGCAGTGCAAAGAAAC TGAAAATAGAGCCCTCTGGCTGGGATGTTTCAGGACAGAGCAGCAACGACAAATACTATACCCACAGCAA AACCCTCCCAGCTACACAAGGGCAAGCCAGCTCCTCTCACCAGGTAGCAAATTTCAATCTTCCTGCTTAC GACCAGGGCCTCCTTCTCCCAGCTCCTGCCGTGGAGCATATTGTGGTAACAGCTGCTGATAGCTCAGGCA GCGCCGCTACAGCAACCTTCCAAAGCAGCCAGACCCTGACTCACAGGAGCAACGTTTCTTTGCTTGAGCC ATATCAAAAATGTGGATTGAAGAGAAAGAGTGAGGAAGTGGAGAGCAACGGTAGCGTGCAGATCATAGAA GAACACCCCCCTCTCATGCTGCAGAACAGAACCGTGGTGGGTGCTGCTGCCACGACCACCACTGTGACCA CCAAGAGTAGCAGTTCCAGCGGAGAAGGGGATTACCAGCTGGTCCAGCATGAGATCCTTTGCTCTATGAC CAACAGCTATGAAGTCCTGGAGTTCCTAGGCCGGGGGACATTTGGACAGGTGGCAAAGTGCTGGAAGCGG AGCACCAAGGAAATTGTGGCCATTAAGATCTTGAAGAACCACCCCTCCTATGCCAGACAAGGACAGATTG AAGTGAGCATCCTTTCCCGCCTAAGCAGTGAAAATGCTGATGAGTATAACTTTGTCCGTTCTTATGAGTG TTTTCAGCACAAGAATCATACCTGCCTTGTGTTTGAGATGTTGGAGCAGAACTTGTACGATTTTCTAAAG CAGAACAAGTTTAGCCCACTGCCACTCAAGTACATAAGACCAATCTTGCAGCAGGTGGCCACAGCCCTGA TGAAGCTGAAGAGTCTTGGTCTGATTCATGCTGACCTTAAACCTGAAAACATAATGCTAGTCGATCCAGT TCGCCAACCCTACCGAGTGAAGGTCATTGACTTTGGTTCTGCTAGTCATGTTTCCAAAGCCGTGTGTTCA ACCTACCTGCAATCACGCTACTACAGAGCTCCTGAAATTATCCTTGGATTACCATTCTGTGAAGCTATTG ACATGTGGTCACTGGGCTGTGTAATAGCTGAGCTGTTCCTGGGATGGCCTCTTTATCCTGGTGCTTCAGA ATACGATCAGATTCGCTATATTTCACAAACACAAGGCCTGCCAGCTGAGTATCTTCTCAGTGCCGGAACA AAAACAACCAGGTTTTTTAACAGAGATCCTAATTTGGGGTACCCACTGTGGAGGCTTAAGACACCTGAAG AACATGAATTGGAAACTGGAATAAAGTCAAAAGAAGCTCGGAAGTACATTTTTAACTGTTTAGATGACAT GGCTCAGGTAAATATGTCTACAGACTTAGAGGGGACAGATATGTTAGCAGAGAAAGCAGATCGGAGAGAG TATATTGATCTTCTAAAGAAAATGCTGACGATTGATGCAGATAAGAGAATCACGCCTCTGAAGACTCTTA ACCACCAATTTGTGACGATGAGTCACCTCCTGGACTTTCCTCACAGCAGCCACGTTAAGTCCTGTTTCCA GAACATGGAGATCTGCAAGCGGAGGGTTCACATGTATGACACAGTGAGTCAGATCAAGAGTCCCTTCACT ACACATGTCGCTCCAAATACAAGCACAAATCTAACCATGAGCTTCAGCAACCAGCTCAACACAGTGCACA ATCAGGCCAGTGTTCTAGCTTCCAGCTCTACTGCAGCAGCAGCTACCCTTTCTCTGGCTAATTCAGATGT CTCGCTGCTAAACTACCAATCGGCTTTGTACCCATCGTCGGCAGCGCCAGTTCCTGGAGTTGCCCAGCAG GGTGTTTCCTTACAACCTGGAACCACCCAGATCTGCACTCAGACAGATCCATTCCAGCAAACATTTATAG TATGCCCACCTGCTTTTCAGACTGGACTACAAGCAACAACAAAGCATTCTGGATTCCCTGTGAGGATGGA TAATGCTGTGCCAATTGTACCCCAGGCGCCTGCTGCTCAGCCGCTGCAGATCCAGTCAGGAGTACTCACA CAGCAAGCCTGGCCTGGAGGAACCCAACAAATTCTCCTGCCTTCAGCCTGGCAGCAGCTGCCCGGGGTAG CTCTGCACAACTCTGTCCAGCCTGCTGCAGTGATTCCAGAGGCCATGGGGAGCAGCCAACAGCTAGCTGA CTGGAGGAATGCCCACTCTCATGGCAACCAGTACAGCACTATTATGCAGCAGCCATCTTTGCTGACCAAC CATGTGACCTTGGCCACTGCTCAGCCTCTGAATGTTGGTGTTGCCCATGTTGTCAGACAACAACAGTCTA GTTCCCTCCCTTCAAAGAAGAATAAGCAGTCTGCTCCAGTTTCATCCAAATCCTCTCTGGAAGTCCTGCC TTCTCAAGTTTATTCTCTGGTTGGGAGTAGTCCTCTTCGTACCACATCTTCTTATAATTCCCTAGTTCCT GTCCAAGACCAGCATCAGCCAATCATCATTCCAGATACCCCCAGCCCTCCTGTGAGTGTCATCACTATCC GTAGTGACACTGATGAAGAAGAGGACAACAAATACAAGCCCAATAGCTCGAGCCTGAAGGCGAGGTCTAA TGTCATCAGTTATGTCACTGTCAATGATTCTCCAGACTCTGACTCCTCCCTGAGCAGCCCACATCCCACA GACACTCTGAGTGCTCTGCGGGGCAACAGTGGGACCCTTCTGGAGGGACCTGGCAGACCTGCAGCAGATG GCATTGGCACCCGTACTATCATTGTGCCTCCTTTGAAAACACAGCTTGGCGACTGCACTGTAGCAACACA GGCCTCAGGTCTCCTTAGCAGTAAGACCAAGCCAGTGGCCTCAGTGAGTGGGCAGTCATCTGGATGCTGT ATCACTCCCACGGGGTACCGGGCTCAGCGAGGGGGAGCCAGCGCGGTGCAGCCACTCAACCTTAGCCAGA ACCAGCAGTCATCGTCAGCTTCAACCTCGCAGGAAAGAAGCAGCAACCCTGCTCCCCGCAGACAGCAGGC ATTTGTGGCCCCGCTCTCCCAAGCCCCCTACGCCTTCCAGCATGGCAGCCCACTGCACTCGACGGGGCAC CCACACTTGGCCCCAGCCCCTGCTCACCTGCCAAGCCAGCCTCACCTGTATACGTACGCTGCCCCCACTT CTGCTGCTGCATTGGGCTCCACCAGTTCCATTGCTCATCTGTTCTCCCCCCAGGGTTCCTCAAGGCATGC TGCAGCTTATACCACACACCCTAGCACTCTGGTGCATCAGGTTCCTGTCAGTGTCGGGCCCAGCCTCCTC ACTTCTGCCAGTGTGGCCCCTGCTCAGTACCAACACCAGTTTGCCACTCAGTCCTACATCGGGTCTTCCC GAGGCTCAACAATTTACACTGGATACCCGCTGAGTCCTACCAAGATCAGTCAGTATTCTTACTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231641 representing NM_001301306 Red=Cloning site Green=Tags(s) MASQLQVFSPPSVSSSAFCSAKKLKIEPSGWDVSGQSSNDKYYTHSKTLPATQGQASSSHQVANFNLPAY DQGLLLPAPAVEHIVVTAADSSGSAATATFQSSQTLTHRSNVSLLEPYQKCGLKRKSEEVESNGSVQIIE EHPPLMLQNRTVVGAAATTTTVTTKSSSSSGEGDYQLVQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKR STKEIVAIKILKNHPSYARQGQIEVSILSRLSSENADEYNFVRSYECFQHKNHTCLVFEMLEQNLYDFLK QNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKAVCS TYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGT KTTRFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCLDDMAQVNMSTDLEGTDMLAEKADRRE YIDLLKKMLTIDADKRITPLKTLNHQFVTMSHLLDFPHSSHVKSCFQNMEICKRRVHMYDTVSQIKSPFT THVAPNTSTNLTMSFSNQLNTVHNQASVLASSSTAAAATLSLANSDVSLLNYQSALYPSSAAPVPGVAQQ GVSLQPGTTQICTQTDPFQQTFIVCPPAFQTGLQATTKHSGFPVRMDNAVPIVPQAPAAQPLQIQSGVLT QQAWPGGTQQILLPSAWQQLPGVALHNSVQPAAVIPEAMGSSQQLADWRNAHSHGNQYSTIMQQPSLLTN HVTLATAQPLNVGVAHVVRQQQSSSLPSKKNKQSAPVSSKSSLEVLPSQVYSLVGSSPLRTTSSYNSLVP VQDQHQPIIIPDTPSPPVSVITIRSDTDEEEDNKYKPNSSSLKARSNVISYVTVNDSPDSDSSLSSPHPT DTLSALRGNSGTLLEGPGRPAADGIGTRTIIVPPLKTQLGDCTVATQASGLLSSKTKPVASVSGQSSGCC ITPTGYRAQRGGASAVQPLNLSQNQQSSSASTSQERSSNPAPRRQQAFVAPLSQAPYAFQHGSPLHSTGH PHLAPAPAHLPSQPHLYTYAAPTSAAALGSTSSIAHLFSPQGSSRHAAAYTTHPSTLVHQVPVSVGPSLL TSASVAPAQYQHQFATQSYIGSSRGSTIYTGYPLSPTKISQYSYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301306
ORF Size	3495 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301306.1, NP_001288235.1
RefSeq Size	7914 bp
RefSeq ORF	3498 bp
Locus ID	15257
UniProt ID	O88904
MW	126.6 kDa
Gene Summary	Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.