Synj2 (NM_001290698) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI481647; mKIAA0348; SJ2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231596 representing NM_001290698 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAAGTTGCTCTGGGCTTCTTGCCACGCGGGTGACACACCTATGATAAATTTTGACTTCCATCAGT TTGCCAAAGGTAGGAAGCTAGAGAAATTGGAGAACCTGTTGAGACCTCAGTTACAGCTACACTGGGAAGA CTTCGGCGTGTTTGCGAAGGGCGAGAATGTAAGTCCACGGTTTCAGAAAGGCACTCTGCGGATGAACTGT CTCGACTGTCTGGATAGAACCAACACTGTGCAGTGCTTCATTGCTCTTGAGGTCCTTCATCTGCAGCTTG AGAGCTTGGGGCTAAATTCAAAGCCCATCATTGACCGTTTTGTGGAGTCCTTCAAAGCCATGTGGTCTCT GAATGGGCACAGCCTGAGCAAGGTGTTCACAGGGAGCAGGGCCCTGGAAGGAAAGGCCAAGGTGGGAAAG CTGAAGGATGGGGCCCGATCCATGTCTCGCACCATCCAGTCCAACTTCTTCGACGGGGTGAAGCAGGAGG CCATCAAGCTACTGCTAGTCGGAGATGTCTACAATGAAGAGTCTACAGACAAAGGACGGATGCTGCTGGA CAACACGGCCCTTCTGGCGACCCCCAGGATCTTGAAGGCCATGACAGAACGCCAGTCGGAATTCACGAAT TTCAAGCGCATCCAGATTGCTGTGGGGACCTGGAATGTGAACGGAGGAAAGCAATTCCGTAGCAATCTCC TGGGGACGGCTGAGCTCACGGACTGGCTCCTAGATGCTCCTCAGCTGTCAGGAGCAGTGGACTCCCAGGA TGATGGCAGTCCTGCTGACGTATTTGCCATCGGGTTTGAGGAGATGGTGGAACTGAGTGCGGGAAATATT GTCAATGCCAGCACCACCAACAGGAAGATGTGGGGCGAGCAGCTTCAGAAAGCCATCTCCCGCTCCCATC GGTACATCCTCTTGACCTCCGCACAGCTGGTGGGCGTCTGTCTTTACATCTTTGTACGTCCGTACCACGT CCCGTTCATCAGAGACGTGGCCATCGACACCGTGAAGACCGGCATGGGGGGAAAGGCGGGGAATAAGGGT GCTGTGGGCATCCGCTTCCAGCTCCACAGCACCAGTTTCTGCTTCGTCTGTAGCCACCTGACGGCTGGGC AGTCTCAGGTGAAGGAGAGGAATGAAGACTACCGGGAGATCACGCACAAACTCTCCTTCCCTTCGGGGAG AAACATATTTTCACATGATTACGTGTTTTGGTGTGGCGATTTCAACTACCGTATTGATCTTACTTACGAA GAAGTCTTCTATTTTGTTAAACGCCAAGACTGGAAGAAACTTATGGAATTCGATCAGTTACAGTTACAGA AATCAAGTGGAAAAATTTTTAAAGACTTTCATGAAGGAGCCGTTAACTTCGGACCCACCTACAAGTATGA CGTTGGATCAGCTGCCTACGACACAAGTGACAAGTGCCGTACCCCAGCCTGGACAGACAGGGTGCTGTGG TGGAGGAAGAAGCATCCATATGATAAAACAGCTGGTGAACTCAACCTTCTAGACAGCGATCTAGACGGCG ATCCCCAAATCAGACACACCTGGTCTCCAGGCACTCTGAAATACTACGGCCGTGCAGAGCTGCAGGCGTC TGATCACAGACCTGTGCTGGCCATTGTGGAGGTGGAGGTTCAAGAGGTGGATGTAGGAGCCCGGGAGAGG GTCTTCCAGGAAGTGTCCTCTGTCCAAGGCCCGCTGGATGCCACCGTTGTTGTAAACCTCCAGTCTCCAA CCCTAGAAGAGAAAAATGAATTTCCAGAGGACCTGCGCACAGAACTTATGCAGACTTTGGGGAATTATGG GACGATTATTCTAGTCAGGATCAACCAAGGGCAGATGCTGGTGACGTTTGCAGACAGCCACTCGGCTCTC AGTGTGCTGGATGTGGATGGTATGAAGGTGAAAGGCAGGGCCGTGAAGATTCGACCAAAGACCAAAGATT GGCTAGAAGGCCTGAGAGAGGAGCTTCTCCGGAAGCGAGACAGCATGGCCCCTGTGTCTCCCACCGCCAA CTCCTGCTTGTTGGAGGAGAACTTTGACTTCTCGAGTCTGGACTATGAGTCCGAAGGGGATGTTCTTGAA GAGGATGAAGACTATTTAGTGGATGGGTTTGGCCAGCCTGTAGTCTCAGACAGTGAGCTCGGTGGAGACA ACTCTTCCGACACCATGAGCTCCTTGACACCCGCCAGCAAGTCTCCCGCCCTGGCTAAAAAGAAGCAACA TCCAACATACAAAGCTGGCTTAATGGTGAAGAAGTCAGCCTCAGACGCGTCCATCTCTTCTGGCACTCAT GGACAATATTCCATCTTGCAGACAGCGAAACTTCTCCCAGGAGCACCCCAGCAACCACCCAAGGCTAGAA CTGGAATAAGTAAACCTTACAACGTCAAACAGATCAAAACCACCAACGCTCAGGAGGCAGAAGCAGCTAT CCGGTGTCTCCTGGAAGCTAGCGGAGGGGTCCCGGAATCAGCCCCAGGTGCCATACCCCTGAGAAACCAA GGGTCTTCTAAGCCAGAGGCCACCCTGGGGCCCCCAGCCCTGCCCCGCCGGCCTGCTCCAAGGGTTCCCA CTATGAAGAAACCAACTTTGAGGAGGACAGGAAAGCCCATGTTACCAGAAGAAAACTTTGAGCCACAGCC TGTCCATTTTACAATGGCTTCCCAGGAAATGAACCTTGAGACCCCTCCTCCAATAACAGCTACTCCCATC CCGCCTGTTCCCAAACCAAGAACACTTCAGCCTGGGAAAGGTGTTGAGGGGAGGCCAAGCAGCGGTAAGC CAGAACCAGATGAAGCTCCTTCCGTGACAGGCACTGTGGAGTCACCACCTCCAGAGGCCCAGGAGGCCCC GTCTCTGGCTCCCAAGGTGCCTCCGAGGAGGAAGAAGTCTGCACCAGCGGCCTTCCACCTGCAGGTCCTG CAGAACAACAGCCAAGTTCTCCAGGGCCTCACGTGCTCCAGCAGTTCTCCACCCTCACTGAAGCCCGACA CCCACCCGCTCTGTCTACAAGTGGCACTTGGCACTTCATCTGCCAGAAGCCCTGAAACCCATGGCCCCAG AGTGACAGAGCCCGAGGCGGCCTCTTTTCATGGCAACTATCCAGATCCCTTCTGGAGCCTTCTCCACCAC CCTAAGCTGTTGAACAATACCTGGCTGTCCAAGAGCTCCGAGCCTTTAGACGTGGGGTCCAGGAACCCTG AGAGGACACACACAGAGCCGGCACAGGTCAATGCATCGTTGGCTGAGAGGGGGCTTCCACCAGACCATGG GGGAAAAGACTTAAGTCACTGGGTGACAGCTAGTAACAAAGACAAGAGGACAACATTAGGTGTT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231596 representing NM_001290698 Red=Cloning site Green=Tags(s) MQKLLWASCHAGDTPMINFDFHQFAKGRKLEKLENLLRPQLQLHWEDFGVFAKGENVSPRFQKGTLRMNC LDCLDRTNTVQCFIALEVLHLQLESLGLNSKPIIDRFVESFKAMWSLNGHSLSKVFTGSRALEGKAKVGK LKDGARSMSRTIQSNFFDGVKQEAIKLLLVGDVYNEESTDKGRMLLDNTALLATPRILKAMTERQSEFTN FKRIQIAVGTWNVNGGKQFRSNLLGTAELTDWLLDAPQLSGAVDSQDDGSPADVFAIGFEEMVELSAGNI VNASTTNRKMWGEQLQKAISRSHRYILLTSAQLVGVCLYIFVRPYHVPFIRDVAIDTVKTGMGGKAGNKG AVGIRFQLHSTSFCFVCSHLTAGQSQVKERNEDYREITHKLSFPSGRNIFSHDYVFWCGDFNYRIDLTYE EVFYFVKRQDWKKLMEFDQLQLQKSSGKIFKDFHEGAVNFGPTYKYDVGSAAYDTSDKCRTPAWTDRVLW WRKKHPYDKTAGELNLLDSDLDGDPQIRHTWSPGTLKYYGRAELQASDHRPVLAIVEVEVQEVDVGARER VFQEVSSVQGPLDATVVVNLQSPTLEEKNEFPEDLRTELMQTLGNYGTIILVRINQGQMLVTFADSHSAL SVLDVDGMKVKGRAVKIRPKTKDWLEGLREELLRKRDSMAPVSPTANSCLLEENFDFSSLDYESEGDVLE EDEDYLVDGFGQPVVSDSELGGDNSSDTMSSLTPASKSPALAKKKQHPTYKAGLMVKKSASDASISSGTH GQYSILQTAKLLPGAPQQPPKARTGISKPYNVKQIKTTNAQEAEAAIRCLLEASGGVPESAPGAIPLRNQ GSSKPEATLGPPALPRRPAPRVPTMKKPTLRRTGKPMLPEENFEPQPVHFTMASQEMNLETPPPITATPI PPVPKPRTLQPGKGVEGRPSSGKPEPDEAPSVTGTVESPPPEAQEAPSLAPKVPPRRKKSAPAAFHLQVL QNNSQVLQGLTCSSSSPPSLKPDTHPLCLQVALGTSSARSPETHGPRVTEPEAASFHGNYPDPFWSLLHH PKLLNNTWLSKSSEPLDVGSRNPERTHTEPAQVNASLAERGLPPDHGGKDLSHWVTASNKDKRTTLGV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290698
ORF Size	3354 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290698.1, NP_001277627.1
RefSeq Size	4779 bp
RefSeq ORF	3357 bp
Locus ID	20975
UniProt ID	Q9D2G5
MW	124 kDa
Gene Summary	Inositol 5-phosphatase which may be involved in distinct membrane trafficking and signal transduction pathways. May mediate the inhibitory effect of Rac1 on endocytosis (By similarity).[UniProtKB/Swiss-Prot Function]

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