Atg9a (NM_001288612) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Apg9l1; Atg9; Atg9l1; AU019532
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231237 representing NM_001288612 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAGTTTGACACTGAATACCAGCGCCTAGAGGCCTCCTATAGCGATTCGCCCCCCGGGGAAGAGG ACCTGTTGGTGCATGTGGCCGAGGGGAGCAAATCACCTTGGCACCACATTGAAAACCTTGACCTCTTCTT CTCTCGAGTTTATAATCTCCACCAGAAGAATGGCTTCACATGTATGCTCATTGGAGAGATGTTTGAACTC ATGCAGTTCCTCTTTGTGGTTGCCTTCACCACCTTCCTGGTTAGCTGTGTGGACTATGACATCCTCTTTG CCAACAAGATGGTGAACCACAGTCTCCATCCTACTGAGCCTGTCAAGGTCACTCTGCCAGATGCCTTTTT GCCTGCCCAAGTCTGTAGTGCCAGGATTCAGGAAAATGGCTCTCTTATCACCATCCTGGTCATTGCTGGT GTCTTCTGGATCCACCGGCTTATCAAGTTTATCTATAACATTTGCTGCTATTGGGAGATCCACTCCTTCT ACCTACATGCTCTTCGGATCCCAATGTCTGCCCTTCCATACTGCACATGGCAGGAAGTTCAGGCCCGGAT TGTGCAGACGCAGAAAGAGCATCAGATCTGCATCCACAAGCGTGAGCTGACAGAGTTGGACATCTACCAT CGCATCCTACGTTTCCAGAACTACATGGTGGCCCTGGTGAACAAATCCCTCCTGCCTCTGCGCTTCCGTC TGCCCGGCCTCGGAGAGGTTGTCTTCTTCACCCGTGGCCTCAAGTACAACTTTGAGCTCATCCTCTTCTG GGGACCCGGCTCTCTGTTTCTCAATGAGTGGAGCCTCAAGGCCGAGTACAAACGTGGAGGGCAACGGCTA GAGCTGGCCCAGCGTCTCAGCAACCGCATCTTGTGGATTGGCATCGCCAACTTCCTGCTGTGTCCCCTCA TCCTCATCTGGCAGATCCTCTATGCCTTCTTCAGCTATGCCGAGGTGCTGAAGAGAGAGCCGGGGGCCCT GGGAGCGCGTTGCTGGTCACTCTATGGCCGTTGTTACCTCCGCCACTTCAATGAGCTGGAGCATGAGCTG CAGTCCCGCCTCAACCGAGGCTACAAGCCCGCCTCCAAGTACATGAATTGCTTCTTGTCACCGCTGCTGA CTCTGCTGGCCAAGAATGGTGCCTTCTTCGCTGGCTCTATCCTGGCTGTGCTTATTGCCCTCACCATCTA TGATGAAGATGTGTTAGCTGTGGAACACGTCCTCACCACGGTCACCCTCCTGGGAGTCACGGTGACTGTG TGCAGGTCCTTCATCCCAGACCAGCACATGGTGTTCTGCCCCGAGCAGCTGCTCCGAGTGATTCTTGCAC ACATCCACTACATGCCTGACCACTGGCAGGGTAATGCCCACCGCTCGCAGACCCGGGACGAGTTTGCCCA GCTCTTCCAGTACAAGGCAGTGTTCATCTTGGAGGAGTTGCTGAGTCCCATCGTCACACCCCTCATTCTC ATCTTCTGCCTCCGCCCTCGGGCCCTGGAGATCATAGACTTCTTCCGCAACTTTACGGTCGAGGTCGTGG GTGTGGGAGACACCTGCTCCTTTGCTCAGATGGACGTTCGCCAGCATGGCCATCCTCAGTGGCTGTCTGG AGGGCAGACAGAGGCCTCAGTGTACCAGCAAGCCGAGGACGGGAAGACCGAGTTGTCGCTCATGCACTTT GCCATCACCAATCCCGGCTGGCAGCCCCCTCGTGAGAGCACAGCTTTCCTGGGCTTCCTCAAGGAGCAGG TGCAGCGAGATGGAGCAGCTGCTGGCCTGGCCCAGGGTGGCCTGCTCCCCGAGAATGCCCTCTTCACATC CATCCAGTCCTTACAGTCTGAGTCCGAGCCACTGAGCCTTATTGCAAATGTGGTAGCAGGCTCATCCTGC CGAGGACCCTCACTGTCCAGAGACCTGCAGGGCTCCAGGCACAGGGCTGATGTTGCTTCTGCCCTTCGAT CCTTCTCCCCTCTGCAGCCTGGAGCGGCCCCTCAAGGCCGGGTTCCCAGTACCATGACAGGCTCTGGAGT GGATGCCAGGACAGCCAGCTCTGGGAGTAGCGTGTGGGAAGGACAGCTGCAGAGCCTGGTGCTGTCCGAA TACGCGTCCACCGAGATGAGCCTGCACGCCCTGTACATGCACCAGCTCCACAAGCAGCAGACCCAGGCTG AGCCCGAGCGGCATGTGTGGCACCGCCGGGAGAGTGATGAGAGTGGAGAGAGTGCCCCTGAAGAGGGGGG AGAGGGTGCCCGGGCCCCCCAACCCATCCCCCGCTCGGCCAGCTATCCCTGTGCTACACCCCGGCCTGGA GCACCTGAGACCACCGCCCTGCATGGGGGCTTCCAGAGGCGCTACGGGGGCATCACAGATCCTGGCACAG TGCCCCGTGGCCCCTCTCACTTCTCCAGGCTGCCCCTGGGAGGATGGGCAGAAGATGGCCAGCCAGCATC AAGGCACCCAGAGCCGGTGCCAGAGGAGGGCTCAGAGGATGAACTCCCCCCTCAAGTGCACAAGGTA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231237 representing NM_001288612 Red=Cloning site Green=Tags(s) MAQFDTEYQRLEASYSDSPPGEEDLLVHVAEGSKSPWHHIENLDLFFSRVYNLHQKNGFTCMLIGEMFEL MQFLFVVAFTTFLVSCVDYDILFANKMVNHSLHPTEPVKVTLPDAFLPAQVCSARIQENGSLITILVIAG VFWIHRLIKFIYNICCYWEIHSFYLHALRIPMSALPYCTWQEVQARIVQTQKEHQICIHKRELTELDIYH RILRFQNYMVALVNKSLLPLRFRLPGLGEVVFFTRGLKYNFELILFWGPGSLFLNEWSLKAEYKRGGQRL ELAQRLSNRILWIGIANFLLCPLILIWQILYAFFSYAEVLKREPGALGARCWSLYGRCYLRHFNELEHEL QSRLNRGYKPASKYMNCFLSPLLTLLAKNGAFFAGSILAVLIALTIYDEDVLAVEHVLTTVTLLGVTVTV CRSFIPDQHMVFCPEQLLRVILAHIHYMPDHWQGNAHRSQTRDEFAQLFQYKAVFILEELLSPIVTPLIL IFCLRPRALEIIDFFRNFTVEVVGVGDTCSFAQMDVRQHGHPQWLSGGQTEASVYQQAEDGKTELSLMHF AITNPGWQPPRESTAFLGFLKEQVQRDGAAAGLAQGGLLPENALFTSIQSLQSESEPLSLIANVVAGSSC RGPSLSRDLQGSRHRADVASALRSFSPLQPGAAPQGRVPSTMTGSGVDARTASSGSSVWEGQLQSLVLSE YASTEMSLHALYMHQLHKQQTQAEPERHVWHRRESDESGESAPEEGGEGARAPQPIPRSASYPCATPRPG APETTALHGGFQRRYGGITDPGTVPRGPSHFSRLPLGGWAEDGQPASRHPEPVPEEGSEDELPPQVHKV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288612
ORF Size	2517 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288612.1, NP_001275541.1
RefSeq Size	3859 bp
RefSeq ORF	2520 bp
Locus ID	245860
UniProt ID	Q68FE2
MW	94.4 kDa
Gene Summary	Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H (By similarity). Required for carbonyl cyanide m-chlorophenylhydrazone (CCCP)-induced ATG8 family proteins lipidation, a key autophagy step.[UniProtKB/Swiss-Prot Function]

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