Cux1 (NM_001291239) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CDP; Cutl1; Cux; Cux-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231090 representing NM_001291239 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCGCGTCAGGTCCGCTCCGAGCTGCCTTCTCCAGCCCGGTTCTCGAAAGCAAAGCCCCGGGGG CCCCGCTGGACTGTAAGGAGACTGATTGTGGTTCCTCGTCGCCTCGAGGGGATGGTATCGGGGCTTCCCA ATCCGCGGCGGAGCTGCCTGCGTGCCCAGAGGATGCTATTCTGCCTGCCACCCCTCATCGGAACCAGCTG CTCGCCCTCGGGGTCCTCCCTGCTAGTGCTAGCTCCGCGTTCGCGGCGCCTGGACAGCCCCAGGATTCTG CCAGGTGGATGTTGTGCGTAGCCGGAGCCAAGTTGAAGAGAGAACTTGATGCCACCGCAACAGTATTGGC AAACAGGCAAGATGAGAGCGAACAGTCCAGAAAGCGGCTCATTGAGCAGAGCCGAGAATTCAAGAAGAAC ACTCCAGAGGATTTACGCAAGCAGGTAGCACCACTGCTAAAGAGCTTCCAAGGGGAGATTGATGCACTGA GTAAAAGAAGCAAAGAAGCAGAGGCAGCCTTCTTGACTGTGTACAAGAGACTAATTGATGTTCCAGATCC GGTACCAGCCCTGGACGTCGGGCAACAGCTGGAAATAAAAGTGCAGCGTCTACACGACATTGAAACAGAG AACCAGAAACTTAGGGAAACACTAGAAGAGTACAACAAGGAGTTTGCTGAAGTGAAAAATCAAGAGGTTA CGATAAAAGCACTTAAGGAGAAAATCCGAGAATACGAGCAGACCCTGAAGAGTCAGGCCGAGACCATTGC TCTGGAGAAAGAGCAGAAGCTACAAAATGATTTTGCAGAGAAGGAGAGAAAGCTGCAAGAGACACAGATG TCCACCACCTCAAAACTGGAGGAAGCTGAGCACAAACTCCAGACTCTGCAAACAGCCCTGGAAAAAACTC GAACAGAATTATTTGACCTGAAAACCAAATATGATGAAGAAACTACTGCAAAGGCCGATGAGATCGAGAT GATCATGACCGACCTTGAACGAGCCAACCAGAGGGCAGAGGTGGCACAGAGAGAAGCAGAGACTTTAAGG GAACAGCTCTCATCGGCCAACCACTCTCTCCAACTGGCCTCGCAGATCCAGAAGGCTCCAGATGTGGCCA TAGAGGTGCTGACCCGATCCAGCCTAGAAGTAGAGTTGGCTGCCAAAGAGCGGGAGATCGCCCAGCTGGT GGAAGATGTGCAGCGACTCCAGGCCAGCCTCACCAAGCTACGTGAGAATTCCGCCAGCCAGATCTCACAG CTGGAGCAGCAACTGAATGCCAAGAATAGCACACTCAAACAACTGGAAGAAAAACTCAAAGGCCAGGCTG ACTATGAAGAAGTGAAGAAAGAGCTGAACACCCTGAAGTCCATGGAGTTTGCACCATCGGAGGGAGCAGG GACACAGGACTCTACCAAGCCCCTGGAGGTTTTACTCCTGGAGAAGAACCGCTCGCTGCAGTCCGAGAAT GCCACGCTGCGCATCTCCAACAGTGACCTGAGCGGGCGCTGTGCGGAGCTGCAGATCCACCTCACTGAGG CCACAGCCAAGGCTGTTGAGCAGAAGGAGCTGATCGCTCGCTTGGAGCAGGACCTCAGCACCATCCAGTC CATCCAACGGCCTGATGCCGAGGGAGCTTCCGAGCAAGGCCTAGAGAAGATTCCAGAACCCATCAAGGAA GCTACAGCTCTGTTCTATGGACCCTCAATGTCATCCAGTGGGACCCTTCCAGAAGGCCAGGTGGACTCCC TGCTTTCCATCATCTCCAGCCAAAGGGAACGTTTCCGCACCCGGAACCAAGAGCTGGAAGCCGAGAGCCG CATGGCCCAGCACACCATCCAGGCCCTGCAGAGCGAGCTGGACAGCCTGCGCGCTGACAACATCAAACTC TTTGAGAAGATCAAGTTCCTGCAGAGTTACCCTGGCAGAGGTATCGGCAGTGACGACACGGAGCTGCGAT ACTCCTCCCAATACGAGGAACGCCTGGACCCTTTCTCCTCCTTCAGCAAGAGGGAGCGGCAGAGGAAGTA CCTGGGCCTGAGCCCCTGGGACAAGGCCACACTTGGCATGGGCCGTCTGATTCTCTCCAACAAGATGGCC CGCACCATCAGCTTCTTTTACACCTTGTTCCTGCACTGCCTGGTCTTTCTGGTGCTGTACAAGCTGGCAT GGAGTGAGAGTGTGGAGAGAGACTGTGCTGCCACCTGCGCCAAGAAGTTCGCCGATCATCTGCACAAATT CCACGAGAGTGACAACGGAGCAGCAGCTGGTGACTTATGGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231090 representing NM_001291239 Red=Cloning site Green=Tags(s) MERASGPLRAAFSSPVLESKAPGAPLDCKETDCGSSSPRGDGIGASQSAAELPACPEDAILPATPHRNQL LALGVLPASASSAFAAPGQPQDSARWMLCVAGAKLKRELDATATVLANRQDESEQSRKRLIEQSREFKKN TPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLTVYKRLIDVPDPVPALDVGQQLEIKVQRLHDIETE NQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKSQAETIALEKEQKLQNDFAEKERKLQETQM STTSKLEEAEHKLQTLQTALEKTRTELFDLKTKYDEETTAKADEIEMIMTDLERANQRAEVAQREAETLR EQLSSANHSLQLASQIQKAPDVAIEVLTRSSLEVELAAKEREIAQLVEDVQRLQASLTKLRENSASQISQ LEQQLNAKNSTLKQLEEKLKGQADYEEVKKELNTLKSMEFAPSEGAGTQDSTKPLEVLLLEKNRSLQSEN ATLRISNSDLSGRCAELQIHLTEATAKAVEQKELIARLEQDLSTIQSIQRPDAEGASEQGLEKIPEPIKE ATALFYGPSMSSSGTLPEGQVDSLLSIISSQRERFRTRNQELEAESRMAQHTIQALQSELDSLRADNIKL FEKIKFLQSYPGRGIGSDDTELRYSSQYEERLDPFSSFSKRERQRKYLGLSPWDKATLGMGRLILSNKMA RTISFFYTLFLHCLVFLVLYKLAWSESVERDCAATCAKKFADHLHKFHESDNGAAAGDLWQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291239
ORF Size	2283 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291239.1, NP_001278168.1
RefSeq Size	3363 bp
RefSeq ORF	2286 bp
Locus ID	13047
MW	85.7 kDa
Gene Summary	Transcription factor involved in the control of neuronal differentiation in the brain. Regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III (PubMed:20510857). Also involved in the control of synaptogenesis (Probable). In addition, it has probably a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator.[UniProtKB/Swiss-Prot Function]

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